scholarly journals Co-Infections of Tilapia Lake Virus, Aeromonas hydrophila and Streptococcus agalactiae in Farmed Red Hybrid Tilapia

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2141
Author(s):  
Lukman Basri ◽  
Roslindawani Md. Nor ◽  
Annas Salleh ◽  
Ina Salwany Md. Yasin ◽  
Mohd Zamri Saad ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Resistance Index ◽  
Inflammatory Cells ◽  
Sequencing Analysis ◽  
Phylogenetic Tree Analysis ◽  
High Death Rate ◽  
High Death ◽  
Intracytoplasmic Inclusion Bodies ◽  
Loss Of Appetite

A high death rate among red hybrid tilapias was observed in a farm in Selangor, Malaysia, in January 2020. The affected fish appeared lethargic, isolated from schooling group, showed loss of appetite, red and haemorrhagic skin, exophthalmia and enlarged gall bladders. Histopathological assessment revealed deformation of kidney tubules, and severe congestion with infiltrations of inflammatory cells in the brains and kidneys. Syncytial cells and intracytoplasmic inclusion bodies were occasionally observed in the liver and brain sections. Tilapia Lake Virus (TiLV), Aeromonas hydrophila and Streptococcus agalactiae were identified in the affected fish, either through isolation or through PCR and sequencing analysis. The phylogenetic tree analysis revealed that the TiLV strain in this study was closely related to the previously reported Malaysian strain that was isolated in 2019. On the other hand, A. hydrophila and S. agalactiae were closer to Algerian and Brazilian strains, respectively. The multiple antibiotic resistance index for A. hydrophila and S. agalactiae was 0.50 and 0.25, respectively. Co-infections of virus and bacteria in cultured tilapia is a new threat for the tilapia industry.

scholarly journals Extract from phyllosphere bacteria with antibiofilm and quorum quenching activity to control several fish pathogenic bacteria

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Olivia Nathalia ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Quorum Quenching ◽  
Indicator Bacteria ◽  
Antibiofilm Activity ◽  
Fish Pathogen ◽  
Phyllosphere Bacteria

Abstract Objective The objective of this research were to screen quorum quenching activity compound from phyllosphere bacteria as well as antibiofilm activity against several fish pathogen bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi. Results We found eight phyllosphere bacteria isolates with potential quorum quenching activity to inhibit Chromobacterium violaceum as indicator bacteria. Crude extracts (20 mg/mL) showed various antibiofilm activity against fish pathogenic bacteria used in this study. Isolate JB 17B showed the highest activity to inhibit biofilm formation of A. hydrophila and V. harveyi, meanwhile isolate JB 3B showed the highest activity to inhibit biofilm of S. agalactiae. From destruction assay, isolate JB 8F showed the highest activity to disrupt biofilm of A. hydrophila isolate JB 20B showed the highest activity to disrupt biofilm of V. harveyi, isolate JB 17B also showed the highest activity to disrupt biofilm of S. agalactiae.

scholarly journals Ablation of Liver X receptors α and β leads to spontaneous peripheral squamous cell lung cancer in mice

2016 ◽  
Vol 113 (27) ◽  
pp. 7614-7619 ◽  
Author(s):  
Yu-bing Dai ◽  
Yi-fei Miao ◽  
Wan-fu Wu ◽  
Yu Li ◽  
Francesca D'Errico ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Squamous Cell ◽  
Cell Lung Cancer ◽  
Squamous Metaplasia ◽  
Inflammatory Cells ◽  
Squamous Cell Lung Cancer ◽  
Alveolar Wall ◽  
Sequencing Analysis ◽  
Thyroid Transcription Factor 1 ◽  
M1 Macrophage

The etiology of peripheral squamous cell lung cancer (PSCCa) remains unknown. Here, we show that this condition spontaneously develops in mice in which the genes for two oxysterol receptors, Liver X Receptor (LXR) α (Nr1h3) and β (Nr1h2), are inactivated. By 1 y of age, most of these mice have to be euthanized because of severe dyspnea. Starting at 3 mo, the lungs of LXRα,βDko mice, but not of LXRα or LXRβ single knockout mice, progressively accumulate foam cells, so that by 1 y, the lungs are covered by a “golden coat.” There is infiltration of inflammatory cells and progressive accumulation of lipid in the alveolar wall, type 2 pneumocytes, and macrophages. By 14 mo, there are three histological lesions: one resembling adenomatous hyperplasia, one squamous metaplasia, and one squamous cell carcinoma characterized by expression of transformation-related protein (p63), sex determining region Y-box 2 (Sox2), cytokeratin 14 (CK14), and cytokeratin 13 (CK13) and absence of thyroid transcription factor 1 (TTF1), and prosurfactant protein C (pro-SPC). RNA sequencing analysis at 12 mo confirmed a massive increase in markers of M1 macrophages and lymphocytes. The data suggest a previously unidentified etiology of PSCCa: cholesterol dysregulation and M1 macrophage-predominant lung inflammation combined with damage to, and aberrant repair of, lung tissue, particularly the peripheral parenchyma. The results raise the possibility that components of the LXR signaling may be useful targets in the treatment of PSCCa.

scholarly journals MicroRNAs Which Can Prognosticate Aggressiveness of Bladder Cancer

Cancers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1551 ◽  
Author(s):  
Edyta Marta Borkowska ◽  
Tomasz Konecki ◽  
Michał Pietrusiński ◽  
Maciej Borowiec ◽  
Zbigniew Jabłonowski
Keyword(s):  
Bladder Cancer ◽  
Area Under The Curve ◽  
Roc Curves ◽  
Control Group ◽  
Low Grade ◽  
Operating Characteristics ◽  
High Death Rate ◽  
Biological Potential ◽  
High Death ◽  
Muscle Invasive

Bladder cancer (BC) is still characterized by a very high death rate in patients with this disease. One of the reasons for this is the lack of adequate markers which could help determine the biological potential of the tumor to develop into its invasive stage. It has been found that some microRNAs (miRNAs) correlate with disease progression. The purpose of this study was to identify which miRNAs can accurately predict the presence of BC and can differentiate low grade (LG) tumors from high grade (HG) tumors. The study included 55 patients with diagnosed bladder cancer and 30 persons belonging to the control group. The expression of seven selected miRNAs was estimated with the real-time PCR technique according to miR-103-5p (for the normalization of the results). Receiver operating characteristics (ROC) curves and the area under the curve (AUC) were used to evaluate the feasibility of using selected markers as biomarkers for detecting BC and discriminating non-muscle invasive BC (NMIBC) from muscle invasive BC (MIBC). For HG tumors, the relevant classifiers are miR-205-5p and miR-20a-5p, whereas miR-205-5p and miR-182-5p are for LG (AUC = 0.964 and AUC = 0.992, respectively). NMIBC patients with LG disease are characterized by significantly higher miR-130b-3p expression values compared to patients in HG tumors.

scholarly journals Causes of spontaneous death and euthanasia in dogs: A background study in Bahia, Brazil

2021 ◽  
Vol 66 (No. 4) ◽  
pp. 156-166
Author(s):  
JL Freitas ◽  
TS Andrade ◽  
ES Martins Filho ◽  
CS D’Soares ◽  
ACSN Souza ◽  
...  
Keyword(s):  
Death Rate ◽  
Northeast Brazil ◽  
Disease Category ◽  
Pathology Laboratory ◽  
High Death Rate ◽  
High Death ◽  
Veterinary Pathology ◽  
Diagnostic Capabilities ◽  
Health Programmes ◽  
Epidemiological Information

Understanding the causes of death in dogs enhances the diagnostic capabilities of veterinarians, as well as reduces animal mortality. Studies on this topic assist in the control and prophylaxis of epidemics and in structuring public health programmes. In total, 1 355 necropsy reports of dogs filed at the Veterinary Pathology Laboratory of the Federal University of Bahia, Northeast, Brazil (2005–2017) were analysed. The epidemiological information and anatomopathological diagnoses were obtained. The diagnosed diseases were classified into 10 categories. The frequency of the zoonoses, the overall age of the death (AD) of the dogs, and the AD of the dogs for each disease category were determined. Infectious and parasitic diseases were the most frequent primary causes of death in puppies (44.62%) and adult dogs (26.52%). In elderly dogs, most deaths occurred due to neoplasms (42.37%). Distemper (10.46%) was the most prevalent condition. A high frequency of zoonoses (6.12%) and a high death rate caused by diseases that could have been prevented (15.06%), such as distemper, parvovirus and canine visceral leishmaniasis, were reported. The AD in the population studied was eight years. The results confirmed the hypothesis of a high death rate in dogs in the state of Bahia, Northeast, Brazil, because of preventable infectious diseases.

scholarly journals DECLARATION OF EPIDEMIC PUBLIC HEALTH WITH NOVEL CORONAVIRUS

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Rafid A. Abdulkareem
Keyword(s):  
Public Health ◽  
Early Detection ◽  
Diagnostic Tests ◽  
Death Rate ◽  
Rna Viruses ◽  
Virus Disease ◽  
Respiratory Problems ◽  
High Death Rate ◽  
High Death ◽  
Novel Coronavirus

Coronaviruses (CoV) are broad enveloped RNA viruses in humans and animals are mostly correlated with enteric and respiratory problems. Within the last decades, incidents of high death rate triggered by the spread of CoV from animals to humans have occurred. Certain pathogen city of CoV is known species-dependent as with the extent of infection; Complications typically appear in closely associated human hosts. Epidemiologists assume that the SARS virus arose in bats (natural reservoirs hosts) that were transferred to persons in Wuhan, China. Presently, the available diagnostic tests are aimed at the traditional early detection of the causes of pneumonia, promote disease prevention efforts and collaborate with research laboratories that can conduct pan coronavirus detection or controlled sequencing. No vaccine is sufficient to protect toward coronaviruses. Also there is no clear treatment for corona virus disease. Patients take comprehensive medication in clinics and typically heal on their own after several times. A vaccine can take up to 45 months to develop. Let’s stay safe during this period of time. Keywords: Coronaviruses, Spreading, bats, diagnostic, vaccine

scholarly journals CST: A Multitask Learning Framework for Colorectal Cancer Region Mining Based on Transformer

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Dong Sui ◽  
Kang Zhang ◽  
Weifeng Liu ◽  
Jing Chen ◽  
Xiaoxuan Ma ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Detection Methods ◽  
Detection Accuracy ◽  
Cancer Screen ◽  
Region Detection ◽  
High Death Rate ◽  
High Death ◽  
Colorectal Cancer Screen ◽  
Segmentation Task ◽  
Transformer Model

Colorectal cancer is a high death rate cancer until now; from the clinical view, the diagnosis of the tumour region is critical for the doctors. But with data accumulation, this task takes lots of time and labor with large variances between different doctors. With the development of computer vision, detection and segmentation of the colorectal cancer region from CT or MRI image series are a great challenge in the past decades, and there still have great demands on automatic diagnosis. In this paper, we proposed a novel transfer learning protocol, called CST, that is, a union framework for colorectal cancer region detection and segmentation task based on the transformer model, which effectively constructs the cancer region detection and its segmentation jointly. To make a higher detection accuracy, we incorporate an autoencoder-based image-level decision approach that leverages the image-level decision of a cancer slice. We also compared our framework with one-stage and two-stage object detection methods; the results show that our proposed method achieves better results on detection and segmentation tasks. And this proposed framework will give another pathway for colorectal cancer screen by way of artificial intelligence.

scholarly journals Autonomous robotic fire detection and extinguishing system

2021 ◽  
Vol 2107 (1) ◽  
pp. 012060
Author(s):  
Nivennesh A/L Sathiabalan ◽  
Anis Farihin Mohamed Lokimi ◽  
Ong Zi Jin ◽  
Nur Syazwani Mohd Hasrin ◽  
Aini Syuhada Md Zain ◽  
...  
Keyword(s):  
Human Error ◽  
Average Length ◽  
Fire Detection ◽  
Water Tank ◽  
Water Pump ◽  
Robot System ◽  
Fire Extinguishing ◽  
High Death Rate ◽  
High Death ◽  
Robot Body

Abstract Firefighting is a dangerous job with a high death rate. Robotics is the new way to protect the environment and human lives. This work proposes an autonomous robot system that can inevitably discover fire using the flame sensor and extinguish it. This project includes Arduino UNO, flame sensor, servo motor, motor driver, relay module, Bluetooth HC-06 module, and water pump. Besides, using the push Bluetooth app at the transmitting end, commands are sent to the receiver to control the robot’s movement. The motors are connected to the microcontroller and used to move the robot and sprinkle water on the fire. A water tank and a water pump are mounted on the robot body and automatically detected by the infrared. An ATMEGA328 series microcontroller controls the flame sensor and the whole operation. A motor driver IC, L298N, is interfaced to the microcontroller through which the controller drives the motors. As a result, the robot can detect fire from a distance. The average length for detecting flame is approximately 5.11cm, and Bluetooth transmission is about 300cm. It has the potential to reduce human error and limitations associated with fire extinguishing tasks.

Mapping of PubMed Literature on Early Trends of 2019 Novel Coronavirus (COVID-19)

2020 ◽  
Author(s):  
Kutty Kumar
Keyword(s):  
Data Science ◽  
Time Frame ◽  
Research Organizations ◽  
High Death Rate ◽  
Pubmed Database ◽  
Examination Time ◽  
Production Distribution ◽  
High Death ◽  
Novel Coronavirus ◽  
Distribution Type

Abstract 2019 novel corona virus infection (COVID-19) causes extreme viral pneumonia in people, known to have a high death rate and a similitude in clinical indications with Severe Acute Respiratory Syndrome coronavirus. This investigation intended to study the attributes of distributions on early COVID-19 research through bibliometric analysis. PubMed database was looked on 07, February, 2020 for COVID-19 distributions published during 01st December 2019 to 06th February 2020. Investigation parameters incorporate year of production, distribution type, examples of universal coordinated effort, and research organizations. An aggregate of 62 COVID-19 research distributions were distributed during the examination time frame. The exploration works were distributed from 13 nations, demonstrating the global noteworthiness on coronavirus episode. USA was the biggest supporter, with 24 articles distributed over a range of 2months and 6 days, trailed by England (11 articles). Aftereffects of the investigation will bear some significance with understudies, specialists, curators and data science experts, and will fill in as a pattern for resulting examinations.

Inquiry found surgeon had high death rate for operation

BMJ ◽  
1998 ◽  
Vol 316 (7144) ◽  
pp. 1553-1553 ◽  
Author(s):  
C. Dyer
Keyword(s):  
Death Rate ◽  
High Death Rate ◽  
High Death

Portraits in Stone

2017 ◽  
Author(s):  
Jennifer Van Horn
Keyword(s):  
South Carolina ◽  
Social Network ◽  
Death Rate ◽  
High Death Rate ◽  
High Death ◽  
Stone Carvers

Elite residents of Charleston, South Carolina, sought a unique means of memorializing their dead: gravestones embellished with bust-length depictions of the deceased. Commissioned from stone carvers in Boston, these portrait gravestones reimagined the small, ivory form of the portrait miniature at a public scale suitable for the cemetery. This chapter examines why Charlestonians patronized this type of memorial, tying the gravestones to residents’ horror at the savagery unleashed upon corpses by putrefaction and to their desire to preserve bodies’ former politeness. Considering portrait gravestones along with mourning rituals and coffin construction illuminates the stones’ role as protective containers that kept savagery at bay, an important function given Charleston’s high death rate and steamy climate. Recognizing the memorials’ similarity to boundary markers, such as those erected to mark the Mason Dixon line, illuminates how the gravestones demarcated a space of colonial control. By erecting stone portraits of civil persons, Charlestonians created a social network with incredible permanence.

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