scholarly journals The Physical Activity-Dependent Hematological and Biochemical Changes in School Horses in Comparison to Blood Profiles in Endurance and Race Horses

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1128
Author(s):  
Małgorzata Maśko ◽  
Małgorzata Domino ◽  
Tomasz Jasiński ◽  
Olga Witkowska-Piłaszewicz

Blood testing is one of the most important ways to improve performance, facilitate recovery and monitor the training of endurance and race horses. However, little is known about the physical activity-dependent changes of blood parameters in horses used for pleasure and in riding schools. This study aimed to perform routine blood tests for training monitoring of sport horses in three different horse types of use. Then the values of blood indicators were compared between school, endurance and race horses to find similarities in the physical activity-dependent profile. The study was carried out on 15 endurance, 15 race and 15 school healthy horses who underwent the typical effort for their disciplines. The hemogram parameters, creatine phosphokinase (CPK), aspartate aminotransferase (AST), blood lactate (LAC), and total serum protein (TSP) concentrations were measured using the same protocol and equipment. Measurements of main hematological and biochemical physical activity-dependent parameters were conducted before, immediately after and 30 min after training. In school horses, the physical activity-dependent increase of WBC (40.9%) and CPK (76.4%) was similar to endurance horses, whereas an increase of RBC (19.1%), HGB (18.6%) and HCT (19.4%) were more similar to race horses. The moderate effort-dependent increase of LAC concentration (2775%) was lower than in race horses (7526%) and higher than in endurance horses (390%). Limiting the training or work monitoring assessment of school horses to only the endurance or racing blood profile may result in the omission of significant changes in hematological and biochemical parameters.

2019 ◽  
Vol 11 (3) ◽  
pp. 465
Author(s):  
J. N. Ikpe ◽  
E. C. Oko ◽  
I. C. Vining-Ogu

Heaps of rice husk waste is currently increasing around rice mills in Nigeria. Production cost for broilers has risen tremendously due to high cost of feed ingredients, one of which is Brewer’s Dried Grain (BDG). Rice husk has been identified as a potential feed stuff and can replace BDG in broiler production. The research determined the effect of substituting BDG with bio-fermented rice husk meal on the blood profiles and nutrient retention of the broilers. Four treatment diets were formulated. T1 (control) contained 15% BDG while in T2, T3 and T4 bio-fermented rice husk meal replaced BDG at 33.33%, 66.67% and 100% to be 5%, 10% and 15% respectively. The diets were assigned to 120 broilers of 28 days old in a complete randomized design of 10 broilers replicated 3 times. The broilers were reared for 28 days after which blood samples were collected. Nutrient retention of the diets was also analyzed. There was a significant (P < 0.05) reduction in the WBC, MCH, MCHC and serum cholesterol level of broilers on the test ingredient. Nutrient retention showed a significant (P < 0.05) increase in ash while the control and T2 were similar (P > 0.05) in crude protein. The research discovered that though there were significant results in most the hematological and biochemical parameters, but the trend of the results was not suggestive that the difference was due to diet. It was concluded that bio-fermented rice husk meal may not have a negative effect on the blood profile of finisher broilers.


Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 231 ◽  
Author(s):  
Valentina Panettieri ◽  
Stavros Chatzifotis ◽  
Concetta Maria Messina ◽  
Ike Olivotto ◽  
Simona Manuguerra ◽  
...  

This research aimed to evaluate the effects of the inclusion of honey bee pollen (HBP) in meagre (Argyrosoumus regius) juveniles’ diets on growth performance, diet digestibility, intestinal morphology, and immunohistochemistry. Furthermore, stress-related molecular markers and biochemical blood profile of fish were evaluated, together with mineral trace and toxic element concentration in pollen and diets. Specimens of meagre (360) of 3.34 ± 0.14 g initial body weight, were randomly allocated to twelve 500 L circular tanks (30 fish per tank). Four diets were formulated: a control diet and three experimental diets with 1%, 2.5%, and 4% of HBP inclusion. All the growth parameters and crude protein and ether extract digestibility coefficients were negatively linearly affected by increased HBP inclusion (p < 0.0001). Histology of medium intestine showed slight signs of alterations in group HPB1 and HPB2.5 compared to control. Fish from HBP4 group showed severe alterations at the intestinal mucosa level. Immunohistochemical detection of TNF-α in the medium intestine showed the presence of TNF-α+ cells in the lamina propria, which resulted in accordance with the increased level of the TNF-α protein detected by immunoblotting in the liver. This stress situation was confirmed by the increased hepatic level of HSP70 (p < 0.05) in fish fed the HBP4 diet and by the linear decrease of total serum protein levels in HBP-containing diets (p < 0.0001). These negative effects can be related to the ultrastructure of the bee pollen grain walls, which make the bioactive substances unavailable and can irritate the intestine of a carnivorous fish such as meagre.


2003 ◽  
Vol 94 (2) ◽  
pp. 660-667 ◽  
Author(s):  
Gwendolyn F. Elphick ◽  
Benjamin N. Greenwood ◽  
Jay Campisi ◽  
Monika Fleshner

Moderate, habitual physical activity improves health, possibly because of beneficial changes in immune function. For example, physical activity can increase natural killer cell cytotoxicity, T cell proliferation, and macrophage function but has minimal impact on antigen-driven B-2-mediated immunoglobulin (Ig) responses. The following studies tested whether physical activity selectively impacts nonantigen-driven B-1-natural IgM (nIgM) but not antigen-driven B-2 Ig. Adult male, pathogen-free Sprague-Dawley rats in a barrier facility voluntarily ran in wheels from 7 to 56 days or were housed in an enriched environment for 56 days. Rats received either no antigen or keyhole limpet hemocyanin (KLH) to assess the B-2 response. Blood samples assessed serum nIgM, total IgG, total serum protein, anti-KLH IgM, and anti-KLH IgG. Physically active rats had higher serum nIgM after 7 days of running, and nIgM remained elevated over 56 days of running. In contrast, free-wheel running produced no changes in total IgG, total serum protein, anti-KLH IgM, and anti-KLH IgG. Environmental enrichment did not alter immune measures from controls. These results suggest that B-1, not B-2, cell responses are selectively impacted by physical activity. Because nIgM is important in multiple aspects of the immune response, an elevation in this innate humoral component could contribute to improved immunity in physically active organisms.


Animals ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 330 ◽  
Author(s):  
Olga Witkowska-Piłaszewicz ◽  
Piotr Bąska ◽  
Michał Czopowicz ◽  
Magdalena Żmigrodzka ◽  
Jarosław Szczepaniak ◽  
...  

Sport training leads to adaptation to physical effort that is reflected by the changes in blood parameters. In equine endurance athletes, blood testing is accepted as a support in training, however, only the changes before versus after exercise in creatine phosphokinase activity (CPK) and basic blood parameters are usually measured. This study is the first longitudinal investigation of the changes in routinely measured blood parameters and, additionally, serum amyloid A (SAA), during seven months, in Arabian horses introduced to endurance training and competing in events for young horses. It has been determined that CPK, aspartate aminotransferase (AST), packed cell volume (PCV), hemoglobin concentration, red blood cell count (RBC), and concentration of total serum protein (TSP) slightly increased after training sessions and competitions in similar manner. The increase in white blood cell (WBC) count was higher after competitions and SAA increased only after competitions. Total protein concentration was the only parameter that increased with training during a 7-month program. SAA indicated only in the case of heavy effort, and, it thus may be helpful in the monitoring of training in young horses. In an optimal program, its concentration should not increase after a training session but only after heavy effort, which should not be repeated too often.


Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2181
Author(s):  
Silvia Martínez-Miró ◽  
Josefa Madrid ◽  
Miguel José López ◽  
Juan Orengo ◽  
Cristian Jesús Sánchez ◽  
...  

A total of 192 Iberian × Duroc pigs kept under intensive conditions were used to investigate the effects of feeding crude glycerin on growth performance, nutrient digestibility, and blood parameters. Animals were blocked by initial body weight (96.0 ± 11.3 kg) and allotted to pens (16 pigs per pen). Pens were assigned randomly to one of three dietary treatments (four pens per treatment). Dietary treatments contained 0, 5, or 10% of crude glycerin proportionally substituting for wheat (G0, G5, and G10, respectively). Diets were formulated to be isoenergetic and isoaminoacidic. No significant effect of crude glycerin was observed on average daily gain, average feed intake, or feed conversion ratio. The apparent total tract digestibility of dry matter, organic matter, and crude protein was no different between treatments. Total serum protein, albumin, glucose, insulin, and IGF-1 were not affected by glycerin inclusion. In conclusion, crude glycerin up to 100 g/kg can be included in the diets of finishing Iberian crossbred pigs without any negative effect on growth performance, digestibility, or serum metabolic parameters.


2014 ◽  
Vol 38 (1) ◽  
pp. 83-91
Author(s):  
Rajiha A. Al- Naimi

Objectives of this study were to evaluate hematological and biochemical parameters in rats ingested toxic doses of CuSO4.5H2O. Thirty six rats divided into 3 equal groups. First group ingested orally distilled water / day for 3 months and considered as control group , second group was drenched 8 mg /kg B.W /day of CuSO4.5H2O for 3 months and considered as Treated group1 (T1). Third group was drenched 40 mg /kg B.W /day of CuSO4.5H2O for 3 months and considered as Treated group2 (T2). Blood collection was done by direct cardiac puncture every month for hematological and biochemical examinations. Hematological parameters showed that anemia caused by CuSO4.5H2O toxicity was of microcytic hypochromic type with significant increase of neutrophils and decrease of lymphocytes and blood platelets. There was significant increase in biochemical parameters , liver enzymes (GOT,GPT,ALP) and total serum protein . It concluded that CuSO4.5H2O poisoning in rats causes significant changes in blood picture and clinical enzyme


2020 ◽  
Vol 13 (10) ◽  
pp. 2197-2202
Author(s):  
Mohammad Abed Alhussen ◽  
Hamdan Naef ◽  
Yury Anatolyevich Vatnikov

Background and Aim: Respiratory diseases in young cattle are among the significant cattle pathologies that cause considerable economic damage globally. For the treatment of respiratory diseases, coformulated drugs that increase general nonspecific resistance, exhibit adaptogenic and anti-inflammatory properties, and normalize metabolic processes in animals are currently being used. The aim of our study was to investigate the therapeutic efficacy of the complex drug "gentaminoseleferon", used in the treatment of respiratory diseases in calves, especially in Mycoplasma dispar infection. Materials and Methods: The animals were divided into three groups. Calves with the first clinical signs of respiratory pathology were randomly divided into two groups. The first experimental group (n=5) was intramuscularly injected with sulfetrisan at a dose of 5-10 mL/animal once per day for 7 days. The second experimental group (n=5) was given gentaminoseleferon at a dose of 1 mL/10 kg of body weight once per day for 7 days. The drugs were not used in the control group, the "healthy animals (n=5)". Blood samples were taken 10 days before and after treatment and compared between the experimental and healthy calves. The changes in the hematological and biochemical parameters of blood and serum were evaluated. Results: During the recovery process of animals in the experimental groups, a normalization of the hematological and biochemical parameters of blood and serum was noted. Interestingly, in calves of the second experimental group, an increase in the total serum protein content by 2.2% (p<0.05) was recorded in comparison with the first group. The second group, furthermore, showed an increase in Vitamins A, E, and C concentrations by 13.5% (p<0.05), 11.9% (p<0.005), and 15.1% (p<0.0005), respectively, as well as in zinc and iron concentrations by 4.1% (p<0.05) and 9.3% (p<0.0001), respectively. These findings indicate a more pronounced decrease in the inflammatory process in the respiratory system and intensive restoration of metabolism, thereby establishing the high therapeutic efficacy of gentaminoseleferon. Conclusion: Gentaminoseleferon was proven highly effective in the treatment of calves with respiratory illnesses and in restoring homeostasis in the organisms of animals after treatment, as indicated by the normalization of morphological and biochemical blood parameters with a reduction in the recovery time.


Author(s):  
Roy Tushar Kanti ◽  
Saha Samir Kumar

In present study an attempt has been made to investigate the combined impact of sub lethal toxicity of Cypermethrin 25% EC and pH on certain blood parameters of Puntius javanicus. Adult fish were exposed to sublethal concentration (0.36 µg/L) of cypermethrin 25% EC in different pH water i.e. pH 5, pH 6, pH 7, pH 8.5 and pH 9.5 for 15 days. Significant changes in red blood cell (RBCs) count, total white blood cells (WBCs), haemoglobin % (Hb%), haematocrit value, total serum protein and blood glucose level were observed in fish exposed to sub-lethal concentration of cypermethrin. Significant decrease in red blood cell (RBCs) count, haemoglobin % (Hb %), haematocrit value and total serum protein content were found in fish exposed to sub-lethal concentration of cypermethrin in pH 5, pH 6 and pH 9.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. A significant increase in these parameters were documented in fish exposed to sub-lethal concentration of cypermethrin in pH 8.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. On the other hand a significant increase of total white blood cells (WBCs) and blood glucose level were recorded in fish subjected to sub-lethal concentration of cypermethrin in pH 5, pH 6 and pH 9.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. Values of these parameters were decreased significantly in fish exposed to sub-lethal concentration of cypermethrin in pH 8.5, compared to fish exposed to sub-lethal concentration of cypermethrin in pH 7. The values of all studied blood parameters in fish subjected to sub-lethal concentration of cypermethrin in pH 8.5 were almost found to retain the values of control group (pH 7, without toxicant).


2012 ◽  
Vol 36 (2) ◽  
pp. 195-198
Author(s):  
Sabreen Majeed Mohammed Ali

This study was conducted to find out the effect of folic acid at different doses (5 g and 10g/animal daily) on blood parameters in adult male rabbits. The animals were divided into 3 equal groups each group contained five animals. One of them as control and other as treated groups T1 and T2. The experiment lasted four weeks. GroupT1 got folic acid at dose 5 g/daily/animal and GT2 10g/animal. To study the hematological parameters such as [total red blood cell (RBC), total white blood cell (WBC), packed cell volume PCV, hemoglobin concentration (Hb) and using serum to detect total serum protein. The folic acid was causing significant increasing in most of these parameters due to the stimulating effect of it and anti-oxidant properties that protect the cell membrane from free radical. Therefore, the administration of folic acid is useful especially for children, pregnant women and aging people.


2022 ◽  
Vol 21 (1) ◽  
pp. 1-13
Author(s):  
A.SH. SULTAN

 Electrophoretic patterns of serum protein in 12 Arabian race horses acutely infected with Babesia equi revealed a significant decrease in albumin (P<0.01) and beta globulins (P<0.05) where, alpha globulins fractions significantly (P<0.01) increased. No significant (P>0.05) changes were recorded in gamma globulins fractions and total serum protein.


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