scholarly journals Antioxidant and Antiapoptotic Effects of a Turraea fischeri Leaf Extract on Cryopreserved Goat Sperm

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2840
Author(s):  
Soha A. Hassan ◽  
Wael A. Khalil ◽  
Mahmoud A. E. Hassan ◽  
Ahmed I. Yousif ◽  
Omar M. Sabry ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Leaf Extract ◽  
Semen Quality ◽  
Soybean Lecithin ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Transmission Electron ◽  
Total Antioxidant ◽  
Apoptotic Activity ◽  
Apoptosis And Necrosis

This study evaluated the efficacy of Turraea fischeri leaf extract for maintaining the viability of cryopreserved goat sperm. Ejaculated semen was collected from 5 mature Baladi bucks (50–60 kg, 2–4 years of age) and those samples with mass motility ≥ 70% and sperm concentration ≥ 2.5 × 109/mL were selected, pooled, and divided into 4 aliquots. Each aliquot was diluted in Tris-citric-soybean lecithin extender containing a different concentration of T. fischeri leaf extract (0, 125, 250, or 375 µg/mL). Treated semen samples were cooled to 5 °C, transferred to 0.25-mL French straws, and stored in liquid nitrogen (LN2) at −196 °C. After thawing, membrane integrity was examined by transmission electron microscopy, apoptotic activity by Annexin/propidium iodide staining and flow cytometry, and both enzyme activities and antioxidant capacity by spectroscopic assays. The leaf extract at 375 µg/mL significantly improved semen quality as indicated by enhanced total antioxidant capacity, reduced H2O2 concentration, a greater proportion of structurally intact motile sperm, and concomitant reductions in apoptosis and necrosis. The extract also significantly increased the proportion of sperm with a contiguous plasma membrane and intact acrosome (p < 0.05). Furthermore, LC-MS revealed numerous secondary metabolites in the extract that may contribute to sperm cryopreservation.

P–112 Association between dietary total antioxidant capacity and semen quality parameters in male partners of couples attempting fertility

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
G Eslamian ◽  
S Rohani ◽  
N Shoaibinobarian
Keyword(s):  
Antioxidant Capacity ◽  
Dietary Pattern ◽  
Total Antioxidant Capacity ◽  
Semen Quality ◽  
Sperm Count ◽  
Quality Parameters ◽  
Cross Sectional ◽  
Abnormal Sperm ◽  
Total Antioxidant ◽  
Dietary Total Antioxidant Capacity

Abstract Study question Is there any association between dietary total antioxidant capacity (TAC) and semen quality parameters in male partners of couples attempting fertility? Summary answer Greater adherence to diets high in TAC was significantly associated with higher total sperm count, sperm concentration, and sperm motility. What is known already Among multiple factors that affect the etiology of poor semen quality and male infertility, dietary factors have an important contribution. Also, chronic oxidative stress negatively effects semen quality. Whether adherence to the diet rich in antioxidants is associated with better semen quality remains largely unexplored.The concentration of single antioxidant cannot show the total antioxidant power of the diet, therefore the concept of dietary total antioxidant capacity (TAC) was invented.Dietary scores are useful approach to evaluate the degree of adherence to specific dietary pattern and its benefits in regard to health Study design, size, duration This was a cross-sectional study of 350 men from couples attending a fertility center in Tehran, Iran, recruited between June 2015 and September 2019. Men aged 25–50 years with complete dietary data were analyzed. Participants/materials, setting, methods Diet was assessed via a reproducible and valid 168-item semi-quantitative food frequency questionnaire to determine the entire antioxidants of the usual diet in order to calculate dietary TAC. Semen quality was assessed according to World Health Organization 2010 guidelines. The association between dietary TAC and semen parameters were assessed adjusting for potential confounders through multiple logistic regression analysis. Main results and the role of chance The average age of study participants was 34.9 ± 7.6 years and their BMI was 28.5 ± 4.3 kg/m2. Participants in the lower tertile of the dietary TAC were lower educated (p &lt; 0.01), more physically active (p &lt; 0.05), and predominantly had abnormal sperm progressive motility (p &lt; 0.01). In the multivariable adjusted models, men in the lowest tertile of the dietary TAC Score had 2.9 times higher likelihood of having abnormal sperm motility, total sperm count and concentration, compared to men in the highest tertile of the TAC score (p &lt; 0.05). Limitations, reasons for caution The main limitation of our study was its cross-sectional design, limiting our ability to derive causal association. Wider implications of the findings: Our study suggests that dietary pattern comprising mainly of antioxidant nutrients may help improve semen quality. Our results are consistent with previous studies showing that plant-based diet contains higher levels of antioxidants are associated with better measures of semen quality. Trial registration number Not applicable

Relationship between semen quality and seminal plasma cholesterol, triacylglycerols and proteins in the domestic cat

2019 ◽  
Vol 22 (10) ◽  
pp. 882-889 ◽  
Author(s):  
María F García ◽  
Romina Nuñez Favre ◽  
María C Stornelli ◽  
Ramiro Rearte ◽  
María C García Mitacek ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Count ◽  
Sodium Dodecyl ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Domestic Cat ◽  
Sds Page ◽  
The Relationship

Objectives The current study aimed to evaluate the relationship between specific seminal plasma components – cholesterol (CHOL), triacylglycerols (TAG) and total protein (PROT) concentrations – and semen quality in cats. A further aim was to determine the relationship between specific seminal protein bands and semen quality. Methods Thirteen toms, 2–5 years of age, were included. Semen collection was performed by electroejaculation every 4 weeks. Fifty-eight ejaculates were assessed for motility, velocity, volume, sperm concentration, total sperm count, viability, acrosome integrity, plasma membrane integrity and sperm morphology. Samples were divided into two groups: good semen quality (GSQ) and poor semen quality (PSQ). After evaluation, seminal plasma was separated from the sperm by centrifugation and stored at −20°C. CHOL, TAG and PROT concentrations were then assessed and seminal plasma protein profile was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results Seminal plasma CHOL and TAG concentrations, motility, velocity, sperm concentration, total sperm count and sperm morphology were significantly higher in GSQ cats compared with PSQ cats ( P <0.01). Moreover, seminal plasma SDS-PAGE analysis showed an identifiable extra band exclusively in the GSQ group. Conclusions and relevance Data obtained in this study showed that seminal plasma CHOL and TAG concentrations and specific protein bands could be used to improve semen evaluation in toms. In this sense, the 14 kDa protein band could be a valuable marker for semen quality in the cat and should be further investigated. However, more studies are necessary to determine its relationship with fertility.

scholarly journals Effect of Macrophages in Semen on Sperm Quality

2020 ◽  
Author(s):  
Gangxin Chen ◽  
Beihong Zheng
Keyword(s):  
Cell Membrane ◽  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Significant Negative Correlation ◽  
High Group ◽  
Cross Sectional ◽  
Cell Membrane Integrity

Abstract Background: This was a cross-sectional study in China, we analyzed the levels of macrophages(Mφ) in semen. The study evaluated the influence of the levels of Mφ in semen on sperm quality.Methods: The subjects were 78 males between 25 and 35 years old. The samples were divided into a low group(Mφ<6×105/ml) and a high group (Mφ>6×105/ml). Evaluation included consideration of the influencing factors of male semen quality, macrophage concentration, sperm motility, morphology, membrane integrity DNA fragmentation index (DFI), anti-sperm antibodies (AsAb), IL-10, and IL-12 in semen.Results: There was no difference in the physical or chemical indices of the semen, sperm concentration, AsAb, IL-10, or IL-12 between the two groups (P>0.05). The percentage of sperm forward motility (PR%), the rate of normal sperm shape, and the integrity of cell membranes in the low group were higher than those in the high group (P<0.05), while the percentage of sperm inactivity (IM%), the rate of sperm head deformity, the rate of deformity in the neck and middle segment, the sperm malformation index (SDI), the abnormal sperm index (TZI), and the sperm DFI in the low group were lower than those in the high group (P<0.05). The concentration of Mφ in the semen was linearly correlated with sperm concentration, sperm PR%, IM%, sperm normal shape rate, head deformity rate, neck and middle deformity rate, SDI, TZI, sperm DFI, and sperm cell membrane integrity (P<0.05), but there was no linear correlation with IL-10 or IL-12 (P>0.05).Conclusions: The concentration of Mφ in semen had no significant correlation with semen volume or sperm concentration, but it did have a significant negative correlation with sperm motility, sperm morphology, cell membrane integrity, and DNA breakage rate. There was no significant correlation with the concentration of IL-10 or IL-12.

scholarly journals Effect of different fractions of Hibiscus rosa sinensis leaf extract on islets of Langerhans and antioxidant activity in non-obese diabetic (NOD) mouse

2011 ◽  
Vol 3 (2) ◽  
pp. 206-210 ◽  
Author(s):  
Fahmi S. Moqbel ◽  
Prakash R. Naik
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Islets Of Langerhans ◽  
Total Antioxidant Capacity ◽  
Leaf Extract ◽  
Nod Mice ◽  
Cell Number ◽  
Nod Mouse ◽  
Hibiscus Rosa Sinensis ◽  
Total Antioxidant

The present investigation was undertaken to study the effect of Hibiscus rosa sinensis leaf extract fractions on islets of Langerhans, total antioxidant activity in non-obese diabetic (NOD) mouse. Two sets of experiments were conducted. First experiment was designed to test the hypoglycemic properties of different isolated fractions. Five fractions were screened for hypoglycemic property. Fractions F3, F4 and F5 showed hypoglycemic in NOD mice. Second set of experiment was conducted to assess the effects of above fractions on the diameter of islets of Langerhans and on the â-cells number and total antioxidant capacity (TAC). All the fractions had a significant effect in the enhancement of diameter of islets of Langerhans and the â-cell number and antioxidant active (total antioxidant capacity) compared with control NOD mice.

18 EFFECTS OF SKIM MILK ON THE QUALITY AND FERTILITY OF BOAR SEMEN FOLLOWING LIQUID PRESERVATION AT 5°C AND 15°C

2011 ◽  
Vol 23 (1) ◽  
pp. 115 ◽  
Author(s):  
Z. Namula ◽  
R. Kodama ◽  
Y. Kaedei ◽  
F. Tanihara ◽  
V. L. Vien ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Storage Temperature ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Skim Milk ◽  
Control Group ◽  
Boar Semen ◽  
Boar Spermatozoa

Liquid preservation of semen can be an alternative to frozen–thawed semen for artificial insemination. The success of a selection of boar semen extenders has been studied over storage periods of 5 to 7 days. The objective of this study was to evaluate the effects of skim milk on the viability and in vitro fertility of boar spermatozoa preserved in Modena-based extenders at 5°C and 15°C for 2 weeks. A total of 7 ejaculates were collected from one boar. The sperm-rich fraction of each ejaculate was centrifuged and diluted in Modena extenders supplemented with 0 (control), 7.5, and 15 mg mL–1 of dry skim milk. The final sperm concentration was adjusted to 1 × 108 cells mL–1, and then the semen was stored at 5°C and 15°C for 2 weeks. In the first experiment, the motility, viability (live/dead fluorescence viability assay), plasma membrane integrity (hypoosmotic swelling test; HOST), and acrosome integrity (FITC-labelled peanut agglutinin staining) of semen stored for 2 weeks were assessed. In the second experiment, the fertilization of stored semen after 20 h of co-incubation with in vitro matured oocytes and their development were examined. Data were analysed using ANOVA. When the semen was stored at 5°C for 2 weeks, the mean total sperm motility of semen stored with 7.5 and 15 mg mL–1 of dry skim milk was significantly higher than that of semen in the control group (41.4% and 41.5% v. 17.4%; P < 0.05). However, the beneficial effects of skim milk on the sperm motility were not observed in the semen stored at 15°C. Moreover, there were no significant differences in the other parameters of semen quality among the groups in each storage temperature. Significantly higher penetration rates of semen stored with 7.5 and 15 mg mL–1 of dry skim milk were observed in the storage at 5°C (41.1% and 34.8% v. 19.8%; P < 0.05) but not at 15°C (38.9% and 26.0% v. 30.0%; P > 0.05) when compared with the control group. When the semen was stored at 5°C, the development rate to the blastocyst stage of oocytes fertilized with semen stored with 7.5 mg mL–1 of dry skim milk was significantly higher than that with control and 15 mg mL–1 of dry skim milk (15.4% v. 1.1% and 7.8%; P < 0.01). However, there were no significant differences in the development rates of oocytes fertilized with semen stored at 15°C among the groups (9.6–11.9%). In conclusion, our results indicate that the effect of skim milk on the viability and in vitro fertility of liquid-stored boar spermatozoa is dependent on the storage temperature. The addition of 7.5 mg mL–1 of dry skim milk may be effective for the improvement of viability and fertility of semen stored at 5°C but not at 15°C.

14 MORPHOLOGICAL AND FUNCTIONAL PARAMETERS OF ENDANGERED BERMEYA GOAT BREED SEMEN

2008 ◽  
Vol 20 (1) ◽  
pp. 87
Author(s):  
C. O. Hidalgo ◽  
A. Rodríguez ◽  
C. Díez ◽  
D. Martín ◽  
M. Carbajo ◽  
...  
Keyword(s):  
Semen Quality ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Analysis Data ◽  
Computer Assisted ◽  
Genetic Stock ◽  
Term Survival ◽  
Sperm Analysis ◽  
Functional Parameters ◽  
Fresh Semen

The Bermeya goats are an endangered autochthonous breed distributed in the north of Spain. To ensure their genetic diversity and long-term survival, morphological and functional parameters of the semen must be known in order to preserve the current genetic stock in a germplasm bank. The aim of this work was to establish basic characteristics and post-thaw survival of Bermeya goat's semen obtained by electro-ejaculation, that is not well described in the literature. The semen was collected by electro-ejaculation from 7 bucks, 1 to 7 years old, twice per week, for 9 weeks (n = 83). Fresh semen was evaluated for volume (V), concentration (C), motility, morphology, functional integrity of the sperm (spz) membranes (hypoosmotic swelling test; HOST), and acrosome integrity rate (NAR). Individual and progressive sperm motility were analyzed by means of a computer-assisted sperm analysis system (CASA: SCA 2002�, Microptic, Barcelona, Spain) immediately after dilution with the extender at 37�C, and after cooling to 4�C; five fields per sample (diluted to 204 � 106 spz mL–1) were evaluated under a phase contrast microscope (100�). The NAR and morphological abnormalities of sperm head, midpiece, tail, and cytoplasmic droplets were determined by counting 100 spz under 1000�. For freezing, ejaculates with at least 80% motile spz were diluted at 32�C with Krebs-Ringer solution containing 20% egg yolk and 14% glycerol to a final concentration of 400 � 106 spz mL–1, cooled to 4�C for 90 min, aspirated into 0.25-mL plastic straws (IMV�, L'Aigle, France), frozen at 7 cm above liquid nitrogen (LN2) phase for 10 min, and then plunged into the LN2. Straws were thawed in a water bath at 39�C for 30 s for post-thaw survival analysis. Data were analyzed by the GLM and FREQ procedures (SAS; SAS Institute, Inc., Cary, NC, USA) and expressed as means � standard error. Fresh semen characteristics were: V = 1.7 � 0.1 mL; C = 2619 � 106 � 153 spz mL–1; total and progressive motility were 89.0 � 2.1% and 66.9 � 2.1%, respectively. Percentages of head abnormalities were 4.8 � 0.5; midpiece: 3.8 � 0.7; tail: 4.7 � 1.0; cytoplasmic droplets: 8.3 � 0.7; intact acrosome: 91.8 � 0.6; and membrane integrity: 49.2 � 2.1. At 4�C, the % of total motile spz was 62.6 � 1.6, and the post-thaw survival rate was 46.3 � 1.5. There were only individual differences (P < 0.001) between bucks on sperm concentration, head abnormalities, and cytoplasmic droplets. In conclusion, our results indicate that semen quality is related to each individual animal and that electro-ejaculation allows collection of semen of satisfactory quality to use as fresh and for cryopreservation. However, the validity of our results for possible future sperm banking of endangered Bermeya goats semen must be confirmed by field trials.

scholarly journals Effects of dietary eucommia ulmoides leaf extract (ELE) on growth performance, expression of feeding-related genes, activities of digestive enzymes, antioxidant capacity, immunity and cytokines expression of large yellow croaker (Larimichthys crocea) larvae

2021 ◽  
pp. 1-29
Author(s):  
Wenxing Huang ◽  
Chuanwei Yao ◽  
Yongtao Liu ◽  
Ning Xu ◽  
Zhaoyang Yin ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Capacity ◽  
Growth Performance ◽  
Digestive Enzymes ◽  
Leaf Extract ◽  
Inflammatory Factors ◽  
Large Yellow Croaker ◽  
Oxide Content ◽  
Eucommia Ulmoides ◽  
Total Antioxidant

Abstract A 30-day feeding trial was conducted to investigate effects of dietary eucommia ulmoides leaf extract (ELE) on growth performance, activities of digestive enzymes, antioxidant capacity, immunity, expression of inflammatory factors and feeding-related genes of large yellow croaker larvae. Five micro-diets were formulated with supplementation of 0g Kg−1 (the control), 5g Kg−1 (0.5%), 10g Kg−1 (1.0%), and 20g Kg−1 (2.0%) of ELE, respectively. Results showed that the best growth performance was found in larvae fed the diet with 1.0% ELE. Furthermore, ELE supplementation significantly increased the npy expression at 1.0% dosage, while increased ghrelin in larvae at 0.5% dosages. The activity of leucine aminopeptidase in larvae fed the diet with 1.0% ELE was significantly higher than the control, while alkaline phosphatase was significantly up-regulated in larvae fed the diet with 2.0% ELE. A clear increase in total antioxidant capacity in larvae fed the diet with 1.0% ELE was observed, whereas catalase activity was significantly higher in 1.0% and 2.0% ELE supplementation compared to the control. Larvae fed the diet with 1.0% ELE had a significantly higher activities of lysozyme, total nitric oxide synthase and nitric oxide content than the control. Moreover, transcriptional levels of cox-2, il-1β and il-6 were remarkably down-regulated by supplementation of 0.5-1.0% ELE. This study demonstrated that the supplementation of 1.0% ELE in diet could increase the growth performance of large yellow croaker larvae probably by promoting expression of feeding-related genes, enhancing antioxidant capacity and immunity, and inhibiting expression of inflammatory factors.

scholarly journals Testosterone and semen seasonality for the sand tiger shark Carcharias taurus†

2019 ◽  
Vol 102 (4) ◽  
pp. 876-887 ◽  
Author(s):  
Jennifer T Wyffels ◽  
Robert George ◽  
Lance Adams ◽  
Cayman Adams ◽  
Tonya Clauss ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Tiger Shark ◽  
Motile Sperm ◽  
Testosterone Concentration ◽  
Plasma Membrane Integrity ◽  
Carcharias Taurus

Abstract Understanding the fundamental reproductive biology of a species is the first step toward identifying parameters that are critical for reproduction and for the development of assisted reproductive techniques. Ejaculates were collected from aquarium (n = 24) and in situ (n = 34) sand tiger sharks Carcharias taurus. Volume, pH, osmolarity, sperm concentration, motility, status, morphology, and plasma membrane integrity were assessed for each ejaculate. Semen with the highest proportion of motile sperm was collected between April and June for both in situ and aquarium sand tiger sharks indicating a seasonal reproductive cycle. Overall, 17 of 30 semen samples collected from aquarium sharks from April through June contained motile sperm compared to 29 of 29 of in situ sharks, demonstrating semen quality differences between aquarium and in situ sharks. Sperm motility, status, morphology, and plasma membrane integrity were significantly higher (P &lt; 0.05) for in situ compared to aquarium sand tiger sharks. Testosterone was measured by an enzyme immunoassay validated for the species. Testosterone concentration was seasonal for both aquarium and in situ sharks with highest concentrations measured in spring and lowest in summer. In situ sharks had higher (P &lt; 0.05) testosterone concentration in spring than aquarium sharks. This study demonstrated annual reproduction with spring seasonality for male sand tiger sharks through marked seasonal differences in testosterone and semen production. Lower testosterone and poorer semen quality was observed in aquarium sharks likely contributing to the species’ limited reproductive success in aquariums.

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