scholarly journals Blood Biochemical Variables Found in Lidia Cattle after Intense Exercise

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2866
Author(s):  
Francisco Escalera-Valente ◽  
Marta E. Alonso ◽  
Juan M. Lomillos-Pérez ◽  
Vicente R. Gaudioso-Lacasa ◽  
Angel J. Alonso ◽  
...  
Keyword(s):  
Descriptive Analysis ◽  
Venous Blood ◽  
Physical Exertion ◽  
Published Data ◽  
Intense Exercise ◽  
Study Objective ◽  
Blood Biochemical ◽  
Biological Variables ◽  
Metabolic Variables ◽  
Bovine Species

There are limited published data in the bovine species on blood biological variables in response to intense work or after significant physical exertion. Lidia cattle, in addition to their exercise components, have some behavioral agonistic features that make them more susceptible to stress. The bullfight involves stress and exercise so intense that it causes significant changes in some metabolic variables. The study objective was to evaluate changes in blood biological variables in response to intense exercise and stress. After the fight in the arena, and once the bulls were dead (n = 438), blood samples were taken, and some biochemical and hormonal variables were determined in venous blood. A descriptive analysis was performed using the Statistica 8.0. computer program. The mean (±s.d.) results obtained were: total protein (85.8 ± 10.8 g/dL), albumin (3.74 ± 4.3 g/dL), triglycerides (39.65 ± 0.16 mg/dL), cholesterol (2.44 ± 0.03 mmol/L), glucose (22.2 ± 9.6 mmol/L), uric acid (340 ± 80 µmol/L), creatinine (236.9 ± 0.4 µmol/L), urea (5.93 ± 1.27 mmol/L), LDH (2828 ± 1975 IU/L), CK (6729 ± 10,931 IU/L), AST (495 ± 462 IU/L), ALP (90 ± 33 IU/L), GGT (50 ± 34 IU/L), ALT (59 ± 35 IU/L), cortisol (117.5 ± 46.6 nmol/L), and testosterone (20.2 ± 23.8 nmol/L). Most of the measured variables clearly increased; thus, we found severe hyperglycemia and increases in LDH, AST, GGT, and ALT enzymes, particularly in CK. The increases in all these variables are justified by the mobilization of energy sources, tissue/muscle damage, and dehydration due to continued stress and intense exercise.

Reference intervals for venous blood gas measurement in adults

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Kirsty L. Ress ◽  
Gus Koerbin ◽  
Ling Li ◽  
Douglas Chesher ◽  
Phillip Bwititi ◽  
...  
Keyword(s):  
Partial Pressure ◽  
Meta Analysis ◽  
Venous Blood ◽  
Reference Interval ◽  
Reference Intervals ◽  
Published Data ◽  
Blood Gas ◽  
Arterial Blood Gas ◽  
Arterial Blood ◽  
Venous Blood Gas

AbstractObjectivesVenous blood gas (VBG) analysis is becoming a popular alternative to arterial blood gas (ABG) analysis due to reduced risk of complications at phlebotomy and ease of draw. In lack of published data, this study aimed to establish reference intervals (RI) for correct interpretation of VBG results.MethodsOne hundred and 51 adult volunteers (101 females, 50 males 18–70 y), were enrolled after completion of a health questionnaire. Venous blood was drawn into safePICO syringes and analysed on ABL827 blood gas analyser (Radiometer Pacific Pty. Ltd.). A non-parametric approach was used to directly establish the VBG RI which was compared to a calculated VBG RI based on a meta-analysis of differences between ABG and VBGResultsAfter exclusions, 134 results were used to derive VBG RI: pH 7.30–7.43, partial pressure of carbon dioxide (pCO2) 38–58 mmHg, partial pressure of oxygen (pO2) 19–65 mmHg, bicarbonate (HCO3−) 22–30 mmol/L, sodium 135–143 mmol/L, potassium 3.6–4.5 mmol/L, chloride 101–110 mmol/L, ionised calcium 1.14–1.29 mmol/L, lactate 0.4–2.2 mmol/L, base excess (BE) −1.9–4.5 mmol/L, saturated oxygen (sO2) 23–93%, carboxyhaemoglobin 0.4–1.4% and methaemoglobin 0.3–0.9%. The meta-analysis revealed differences between ABG and VBG for pH, HCO3−, pCO2 and pO2 of 0.032, −1.0 mmol/L, −4.2 and 39.9 mmHg, respectively. Using this data along with established ABG RI, calculated VBG RI of pH 7.32–7.42, HCO3− 23 – 27 mmol/L, pCO2 36–49 mmHg (Female), pCO2 39–52 mmHg (Male) and pO2 43–68 mmHg were formulated and compared to the VBG RI of this study.ConclusionsAn adult reference interval has been established to assist interpretation of VBG results.

Agreement between i-STAT and YSI 2300 devices to determine lactate concentrations in dogs undergoing exercise

2016 ◽  
Vol 12 (2) ◽  
pp. 75-82 ◽  
Author(s):  
C. Berkman ◽  
M.C. Pereira ◽  
K.B. Nardi ◽  
G.T. Pereira ◽  
O.A.B. Soares ◽  
...  
Keyword(s):  
Venous Blood ◽  
Lactate Concentration ◽  
Intense Exercise ◽  
Blood Samples ◽  
Handheld Device ◽  
Clinical Evaluations ◽  
Small Constant ◽  
Constant Bias ◽  
Highly Correlated ◽  
The Relationship

Little information is available comparing the i-STAT and the YSI 2300 Stat Plus devices to determine the lactate concentration [Lac] in dogs undergoing intense exercise. The reproducibility of the YSI 2300 for quantifying the [Lac] in canine blood [Lac]b and plasma [Lac]p samples has been observed. In addition, the i-STAT handheld device was used to quantify [Lac] in dogs subjected to exercise, and the results were compared with that of YSI 2300. Venous blood samples of Beagle and American Pit Bull Terrier dogs were obtained during an intense exercise training on a treadmill. [Lac]p and [Lac]b were quantified using the YSI 2300 instrument to determine the reproducibility of the results. A total of 52 specimens were compared for both plasma and whole blood. For comparing the devices (YSI 2300 vs i-STAT), 96 samples were used. Ordinary least products regression, the correlation coefficient, and Bland-Altman plots were used to assess the agreement of using the i-STAT device. The relationship between duplicate measurements of both [Lac]b and [Lac]p by YSI 2300 was strong (r=0.99). A correlation between the data obtained using the i-STAT and YSI 2300 instruments was observed for both the [Lac]p (r=0.97) and [Lac]b (r=0.88). The i-STAT exhibited a small constant bias (-0.25 mmol/l) compared to YSI 2300 ([Lac]b). There were proportional biases of 0.89 mmol/l for [Lac]p and 1.22 mmol/l for [Lac]b when using YSI 2300 vs i-STAT. We confirmed the reproducibility of the YSI 2300 for canine lactate blood/plasma samples. The results obtained by the i-STAT and YSI 2300 analyser were highly correlated, but a small constant bias was observed between them. The i-STAT device can be used in clinical evaluations, and it is also adequate for designing and monitoring fitness programmes.

scholarly journals Comparison of capillary and venous blood for malaria detection using two PCR based assays in febrile patients in Sierra Leone.

2020 ◽  
Author(s):  
Tomasz Leski ◽  
Chris Rowe Taitt ◽  
Umaru Bangura ◽  
Joseph Lahai ◽  
Joseph M. Lamin ◽  
...  
Keyword(s):  
Sierra Leone ◽  
Venous Blood ◽  
Capillary Blood ◽  
Clinical Samples ◽  
Published Data ◽  
Health Control ◽  
Multiplexed Pcr ◽  
Parasite Detection ◽  
Diagnostic Technologies ◽  
Clinical Case Management

Abstract Background. Malaria parasites infect over 200 million people and cause more than 400,000 deaths each year. Rapid and sensitive diagnostics are critical tools for clinical case management and public health control efforts. Both capillary and venous blood are currently used for malaria detection and while diagnostic technologies may not be equally sensitive with both materials, the published data on this subject are scarce and not conclusive.Methods. Paired clinical samples of venous and capillary blood from 141 febrile individuals in Bo, Sierra Leone, were obtained between January and May 2019 and tested for the presence of Plasmodium parasites using two multiplexed PCR assays: the FilmArray-based Global Fever Panel (GFP) and the TaqMan-based Malaria Multiplex Sample Ready (MMSR) assay.Results. We observed no significant differences in Plasmodium parasite detection between capillary and venous blood for both assays. The GFP assay was more sensitive than MMSR for all markers that could be compared (Plasmodium spp. and P. falciparum) in both venous and capillary blood.Conclusions. No difference was found in malaria detection between venous and capillary blood using two different PCR-based detection assays. This data gives support for use of capillary blood, a material which can be obtained easier by less invasive methods, for PCR-based malaria diagnostics, independent of the platform.

Hemodynamics and metabolism of the in vivo vascularly isolated canine pancreas.

1979 ◽  
Vol 236 (6) ◽  
pp. E626
Author(s):  
R J Alteveer ◽  
M J Jaffe ◽  
J Van Dam
Keyword(s):  
Blood Flow ◽  
Glucose Uptake ◽  
Venous Blood ◽  
Fatty Acid Uptake ◽  
Acid Uptake ◽  
Anesthetized Dogs ◽  
Metabolic Variables ◽  
The Stability ◽  
First Time

Surgical procedures are detailed that have yielded for the first time an in vivo vascularly isolated, autoperfused preparation of the entire pancreas in anesthetized dogs. Previous studies had isolated only part of the pancreas or had resorted to blood-flow techniques not requiring pooled pancreatic venous blood, necessary for metabolic studies of the organ. Pancreatic blood flow (48 ml/min), O2 uptake (180 mumol/min), glucose uptake (51.0 mumol/min), lactate output (6.6 mumol/min), net free fatty acid uptake (2.23 mumol/min), all per 100 g tissue, and various other measured and calculated hemodynamic and metabolic variables were determined on the preparation during control conditions. The stability of the preparation was verified by serial determinations of these parameters and of blood alpha-amylase and beta-glucuronidase levels from 1 to 2.5 h postsurgery. Metabolic rate and glucose uptake were both found to be much higher than in intestinal tissues and approached values characteristic of liver tissue.

scholarly journals Arterial hypertension and aortic root dilatation: an unsolved mystery

2013 ◽  
pp. 6-11
Author(s):  
Alberto Milan ◽  
Francesco Tosello ◽  
Sara Abram ◽  
Ambra Fabbri ◽  
Alessandro Vairo ◽  
...  
Keyword(s):  
Arterial Hypertension ◽  
Thoracic Aorta ◽  
Aortic Root ◽  
Aortic Wall ◽  
Aortic Aneurysms ◽  
Published Data ◽  
Thoracic Aortic Aneurysms ◽  
Biological Variables ◽  
Aortic Root Dilatation ◽  
Active Debate

Introduction: Acute and chronic aortic syndromes are associated with substantial morbidity and mortality. Silent risk factors such as arterial hypertension and aortic root dilatation can increase the likelihood of aortic dissection or rupture. The relationship between arterial hypertension and the dimensions of the aortic root dimension is a topic of active debate. Materials and methods: We reviewed the literature on the physiopathology, diagnosis, natural history, and management of thoracic aortic aneurysms. Results: Biological variables influencing the size of the aorta include age, sex, body surface area, pressure values, and stroke volume. Pathologic enlargement of the thoracic aorta can be caused by genetic, degenerative, inflammatory, traumatic, or toxic factors. Studies investigating the correlation between aortic dimensions and arterial pressures (diastolic, systolic, or pulse) have produced discordant results. Discussion: Classically, emphasis has been placed on the importance of hypertension-related degeneration of the medial layer of the aortic wall, which leads to dilatation of the thoracic aorta, reduced aortic wall compliance, and increased pulse pressures. However, there are no published data that demonstrate unequivocally the existence of a pathogenetic correlation between arterial hypertension and aortic root dilatation. Furthermore, there is no evidence that antihypertensive therapy is effective in the management of nonsyndromic forms of aortic root dilatation. An interesting branch of research focuses on the importance of genetic predisposition in the pathogenesis of thoracic aortic aneurysms. Different genetic backgrounds could explain differences in the behaviour of aortic walls exposed to the same hemodynamic stress. Further study is needed to evaluate these focal physiopathological aspects.

Establishing reference intervals of 20 biochemical markers for children in Southwestern Fujian, China based on the UniCel DxC 800 system

2020 ◽  
Vol 57 (6) ◽  
pp. 435-443
Author(s):  
Jiming Li ◽  
Huifen Zhang ◽  
Haichen Chen ◽  
Yuebin Gan ◽  
Juan Li ◽  
...  
Keyword(s):  
Biochemical Markers ◽  
Venous Blood ◽  
Reference Intervals ◽  
Healthy Children ◽  
Gamma Glutamyl Transpeptidase ◽  
Indirect Bilirubin ◽  
Blood Biochemical ◽  
Industry Standard ◽  
Upper Limits ◽  
Calcium Magnesium

Background To date, China has no industry standard for reference intervals of paediatric blood biochemical markers. This study aimed to evaluate changes in biochemical markers in the venous blood of healthy children aged 29 days to 12 years, derived from the UniCel DxC 800 system, and establish appropriate reference intervals. Methods We analysed venous blood from 1980 healthy children for 20 biochemical markers. Reference intervals were established according to the Clinical and Laboratory Standards Institute C28-A3c guideline and compared with those of adults in China. Results All markers except for sodium and chlorine required partitioning by age, but not by sex. The reference intervals of total protein, albumin, globulin, carbon dioxide, urea nitrogen, creatinine and uric acid consistently increased with age in children, but were always lower than those of adults. Children aged 29 days to 12 years had a single combined RI for sodium and chloride, respectively; although the reference intervals in children were similar to those of adults, their upper limits were lower. The reference intervals of direct bilirubin, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, potassium, calcium, magnesium, and phosphorus continued to decline with age. The reference intervals of total bilirubin, indirect bilirubin, and gamma-glutamyl transpeptidase initially declined followed by a slight rebound. Conclusions While establishing reference intervals, most markers required partitioning by age (aged 29 days to 12 years); the partitioning scheme differed for each marker, and paediatric reference intervals differed from those for adults. It is therefore necessary to establish separate paediatric reference intervals.

Hematological Status of Male Runners in Relation to the Extent of Physical Training

1992 ◽  
Vol 2 (4) ◽  
pp. 366-375 ◽  
Author(s):  
John D. Robertson ◽  
Ronald J. Maughan ◽  
Ann C. Milne ◽  
Ronald J.L. Davidson
Keyword(s):  
Serum Ferritin ◽  
Iron Status ◽  
Normal Population ◽  
Venous Blood ◽  
Biological Significance ◽  
Iron Intake ◽  
Dietary Analysis ◽  
Control Subjects ◽  
Blood Biochemical ◽  
Biochemical Indices

Blood biochemical indices of iron status were measured in venous blood from 20 runners and 6 control subjects. All subjects were.male, ages 20 to 40 years, and stable with regard to body weight and degree of physical activity. Dietary analysis was undertaken using a 7-day weighed food intake. There was no evidence of iron deficiency: hemoglobin concentrations and serum femtin levels were within the normal population range for all individuals. However, serum ferritin was negatively correlated with the amount of training. Daily iron intake appeared to be adequate; iron intake was correlated with protein intake but not related to training or energy intake. Serum ferritin, an indicator of iron status, was significantly correlated with vitamin C intake but not iron intake. Serum transferrin concentration was higher in the group of athletes undertaking a high weekly training load compared with the control subjects, suggesting an alteration in iron metabolism although there was no evidence of increased erythropoiesis. The biological significance of this is unclear.

scholarly journals (A70) Traumatic Brain Injuries at a Rural Teaching Hospital: Pattern of Presentation and Documentation

2011 ◽  
Vol 26 (S1) ◽  
pp. s19-s20
Author(s):  
A. Agrawal ◽  
A. Kakani ◽  
N. Baisakhiya ◽  
S. Galwankar ◽  
S. Dwivedi
Keyword(s):  
Traffic Accidents ◽  
Brain Injuries ◽  
Developmental Stages ◽  
Road Traffic ◽  
Descriptive Analysis ◽  
Tertiary Hospital ◽  
Traumatic Brain Injuries ◽  
Published Data ◽  
The Road ◽  
Paper Form

Background and ObjectivesAnalyses of causes and trends of traumatic brain injuries help to define public health policy priorities. There are not much TBI registries, thus making documentation of injuries inadequate and accessing these data problematic. This study is aimed at identifying the characteristics of TBI and determining the efficiency of documentation of patients' records in a tertiary hospital.Patients and MethodsBased on WHO guidelines “Standards for Surveillance of Neurotrauma” we designed a proforma to collect data on traumatic brain injuries. A prospective data collection was done from January to June 2010. Data was collected on a paper form and then entered into the self-developed TBI registry database. Descriptive analysis was performed.ResultsData for a total 414 patients were collected. Mean age was 33.00 years (SD ± 16.725, range 1–85 years), and 81% male. Most of the accidents took place on highways (57.2%), commonest being the road traffic accidents (55.1%), brought by relatives (74%). The mean duration for hospital stay was 5.42 days (SD ± 8.312 days, range 1-79 days). 10% patients required resuscitation at the time of admission. Details of Glasgow coma scale were available; details regarding CT scan findings were available for 300 patients. Good recovery was seen in 68.4% and the mortality was in 7.2%. Further details on vital parameters and investigations included in the study were also collected.ConclusionsTBI related research in many developing countries is in the developmental stages with relatively few published data. Although early analysis of a TBI data can lead to useful information, there is further need for the development of a user-friendly secure web-based database system to continuously maintain and analyze the registry.

scholarly journals Seroprevalence of antibodies against SARS-Cov-2 in the high impacted sub-district in Jakarta, Indonesia

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261931
Author(s):  
Olivia Herlinda ◽  
Adrianna Bella ◽  
Gita Kusnadi ◽  
Dimitri Swasthika Nurshadrina ◽  
Mochamad Thoriq Akbar ◽  
...  
Keyword(s):  
Data Collection ◽  
Local Governments ◽  
Descriptive Analysis ◽  
Venous Blood ◽  
Epidemiological Surveillance ◽  
Age Group ◽  
Strategic Responses ◽  
Protective Behaviours ◽  
Actual Prevalence ◽  
Venous Blood Sampling

Background Understanding the actual prevalence of COVID-19 transmission in the community is vital for strategic responses to the pandemic. This study aims to estimate the actual infection of COVID-19 through a seroprevalence survey and to predict infection fatality rate (IFR) in Tanjung Priok, the hardest-hit sub-district by the COVID-19 in Jakarta, Indonesia. Methods We conducted a venous blood sampling (phlebotomy) to 3,196 individuals in Tanjung Priok between Nov 23, 2020, and Feb 19, 2021 to detect their antibodies against SARS-CoV-2. Using an enumerator-administered questionnaire, we collected data on the respondents’ demographic characteristics, COVID-19 test history, COVID-19 symptoms in the last 14 days, comorbidities, and protective behaviours during the last month. We employed descriptive analysis to estimate the seroprevalence and IFR. Findings The prevalence of Antibody against SARS-CoV-2 was 28.52% (95% CI 25.44–31.81%), with the result being higher in females than males (OR 1.20; 95% CI 1.02–1.42). By the end of the data collection (February 9, 2021), the cumulative cases of COVID-19 in Tanjung Priok were reported to be experienced by 9,861 people (2.4%). Those aged 45–65 were more likely to be seropositive than 15–19 years old (OR 1.42; 95% CI 1.05–1.92). Nearly one third (31%) of the subjects who developed at least one COVID-19 symptom in the last 14 days of the data collection were seropositive. The estimated IFR was 0.08% (95% CI 0.07–0.09), with a higher figure recorded in males (0.09; 95% CI 0.08–0.10) than females (0.07; 95% CI 0.06–0.08), and oldest age group (45–65) (0.21; 95% CI 0.18–0.23) than other younger groups. Conclusion An under-reporting issue was found between the estimated COVID-19 seroprevalence and the reported cumulative cases in Tanjung Priok. More efforts are required to amplify epidemiological surveillance by the provincial and local governments.

scholarly journals A descriptive analysis of the data availability statements accompanying medRxiv preprints and a comparison with their published counterparts

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0250887
Author(s):  
Luke A. McGuinness ◽  
Athena L. Sheppard
Keyword(s):  
Data Sharing ◽  
Descriptive Analysis ◽  
Open Data ◽  
System Change ◽  
Research Data ◽  
Data Availability ◽  
Published Data ◽  
Editorial Policies ◽  
Journal Editors ◽  
Closed Data

Objective To determine whether medRxiv data availability statements describe open or closed data—that is, whether the data used in the study is openly available without restriction—and to examine if this changes on publication based on journal data-sharing policy. Additionally, to examine whether data availability statements are sufficient to capture code availability declarations. Design Observational study, following a pre-registered protocol, of preprints posted on the medRxiv repository between 25th June 2019 and 1st May 2020 and their published counterparts. Main outcome measures Distribution of preprinted data availability statements across nine categories, determined by a prespecified classification system. Change in the percentage of data availability statements describing open data between the preprinted and published versions of the same record, stratified by journal sharing policy. Number of code availability declarations reported in the full-text preprint which were not captured in the corresponding data availability statement. Results 3938 medRxiv preprints with an applicable data availability statement were included in our sample, of which 911 (23.1%) were categorized as describing open data. 379 (9.6%) preprints were subsequently published, and of these published articles, only 155 contained an applicable data availability statement. Similar to the preprint stage, a minority (59 (38.1%)) of these published data availability statements described open data. Of the 151 records eligible for the comparison between preprinted and published stages, 57 (37.7%) were published in journals which mandated open data sharing. Data availability statements more frequently described open data on publication when the journal mandated data sharing (open at preprint: 33.3%, open at publication: 61.4%) compared to when the journal did not mandate data sharing (open at preprint: 20.2%, open at publication: 22.3%). Conclusion Requiring that authors submit a data availability statement is a good first step, but is insufficient to ensure data availability. Strict editorial policies that mandate data sharing (where appropriate) as a condition of publication appear to be effective in making research data available. We would strongly encourage all journal editors to examine whether their data availability policies are sufficiently stringent and consistently enforced.

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