scholarly journals Ruminal In Vitro Protein Degradation and Apparent Digestibility of Energy and Nutrients in Sheep Fed Native or Ensiled + Toasted Pea (Pisum sativum) Grains

Animals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 401 ◽  
Author(s):  
Martin Bachmann ◽  
Christian Kuhnitzsch ◽  
Paul Okon ◽  
Siriwan D. Martens ◽  
Jörg M. Greef ◽  
...  
Keyword(s):  
Protein Solubility ◽  
Streptomyces Griseus ◽  
Metabolizable Energy ◽  
Net Energy ◽  
Apparent Digestibility ◽  
Throughput Rate ◽  
Nitrogen Free Extract ◽  
Ruminant Diets ◽  
Gross Energy

Pea grains may partially replace soybean or rapeseed meals and cereals in ruminant diets, but substitution by unprocessed peas is limited by high ruminal protein solubility. The effect of combined ensiling and toasting of peas using a mobile toaster (100 kg/h throughput rate, 180 to 190 °C supplied air temperature) on rumen-undegraded protein (RUP) was tested in vitro using the Streptomyces griseus protease test. The effects of ensiling plus toasting on apparent digestibility of organic matter (OM), gross energy (GE), and proximate nutrients were examined in a digestion trial. Concentrations of metabolizable energy (ME) and net energy lactation (NEL) were calculated. Native peas had 38 g RUP/kg dry matter (DM), which was 20% of crude protein (CP). Rumen-undegraded protein increased three-fold after ensiling plus toasting (p < 0.001). Acid detergent insoluble protein increased five-fold. Apparent digestibility was 0.94 (OM), 0.90 (CP), and above 0.99 (nitrogen-free extract, starch, and sugars) and was not altered by the treatment. The ME (13.9 MJ/kg DM) or the NEL (8.9 MJ/kg DM) concentration was similar in native and ensiled plus toasted peas. This technique can easily be applied on farms and may increase RUP. However, it needs to be clarified under which conditions pea protein will be damaged.

scholarly journals Estimating digestible energy values of feeds and diets and integrating those values into net energy systems

2018 ◽  
Vol 3 (3) ◽  
pp. 953-961 ◽  
Author(s):  
William P Weiss ◽  
Alexander W Tebbe
Keyword(s):  
Energy System ◽  
Metabolizable Energy ◽  
Neutral Detergent Fiber ◽  
Net Energy ◽  
Nutrient Analysis ◽  
Digestible Energy ◽  
Important Variation ◽  
Negative Effect ◽  
Gross Energy

Abstract The California Net Energy System (CNES) used a combination of measured and tabular metabolizable energy (ME) values and changes in body composition gain to determine net energy requirements for maintenance and gain and their corresponding dietary concentrations. The accuracy of the CNES depends on the accuracy of the feed ME values. Feed or diet ME values can be measured directly but are expensive and require specialized facilities; therefore, most ME values are estimated from digestible energy (DE) values, which are often estimated from the concentration of total digestible nutrients (TDN). Both DE and TDN values are often from tables and not based on actual nutrient analysis. The use of tabular values eliminates important within-feed variation in composition and digestibility. Furthermore, the use of TDN to estimate DE does not account for important variation in the gross energy value of feeds. A better approach would be to estimate DE concentration directly from nutrient composition or in vitro (or in situ) digestibility measurements. This approach incorporates within-feed variation into the energy system and eliminates the issues of using TDN. A widely used summative equation based on the commonly measured feed fractions (ash, crude protein, neutral detergent fiber, and fat) has been shown to accurately estimate DE concentrations of many diets for cattle; however, deficiencies in that equation have been identified and include an overestimation of DE provided by fat and an exaggerated negative effect of intake on digestibility. Replacing the nonfiber carbohydrate term (which included everything that was not measured) in the equation with measured starch concentration and residual organic matter (i.e., nonfiber carbohydrate minus starch) should improve accuracy by accounting for more variation in starch digestibility. More accurate estimates of DE will improve the accuracy of ME values, which will ultimately lead to more accurate NE values.

scholarly journals  Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods

2012 ◽  
Vol 57 (No. 10) ◽  
pp. 454-468 ◽  
Author(s):  
P. Homolka ◽  
V. Koukolová ◽  
M. Podsedníček ◽  
A. Hlaváčková
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Energy Levels ◽  
Red Clover ◽  
Metabolizable Energy ◽  
Net Energy ◽  
Gross Energy ◽  
Model Expression

The aim of this study was to determine the nutrient and energy levels of red clover and lucerne forage. Investigation of forage at different maturity stages of three growths was carried out by chemical analysis, in vitro and in vivo digestibility methods.&nbsp; Generally, maturation caused a significant increase in fibre fractions. With the increasing maturity of forage samples the in vivo, in vitro, and calculated in vivo (in vivo<sub>calcul</sub>) digestibilities of organic matter (OM) linearly decreased. The in vitro and in vivo<sub>calcul</sub> digestibilities of OM averaged 0.754 and 0.708 for red clover and 0.717 and 0.667 for lucerne, respectively. The in vivo OM digestibility averaged 0.710 for red clover and 0.666 for lucerne. Gross energy (GE), digestible energy (DE), metabolizable energy (ME), net energy for lactation (NEL), and net energy for growth (NEG) averaged 18.12, 12.41, 9.60, 5.67, 5.50 and 18.09, 11.56, 9.01, 5.26, 4.99 MJ/kg of dry matter for red clover and lucerne, respectively. The effect of a vegetative stage on energy values of both forages was diverged for various growth times. When data were pooled across the estimated season, seven cutting-specific equations for descriptions of GE, DE, ME, NEL, NEG, in vitro, and in vivo OM digestibilities were obtained for red clover and lucerne, separately. The red clover model expression gave similar prediction equations for lucerne. It was possible to predict cutting-specific equations with coefficients of determination R<sup>2</sup> &gt; 0.719 for red clover and R<sup>2</sup> &gt; 0.400 for lucerne of the variation in GE, DE, ME, NEL, and NEG. The in vitro and in vivo OM digestibility equations were predicted with R<sup>2 </sup>being 0.840 (in vitro) and 0.707 (in vivo) for red clover, and 0.979 (in vitro) and 0.937 (in vivo) for lucerne. The parameters of these specific equations were statistically preferable than the general model expression which included both forages together. &nbsp;

A note on the influence of micronization and polyethylene glycol on the nutritional value of brown sorghum for growing pigs

1980 ◽  
Vol 30 (1) ◽  
pp. 157-160 ◽  
Author(s):  
G. P. Savage ◽  
W. C. Smith ◽  
P. A. Briggs
Keyword(s):  
Polyethylene Glycol ◽  
Crude Protein ◽  
Nutritional Value ◽  
Energy Content ◽  
Nitrogen Retention ◽  
Growing Pigs ◽  
Metabolizable Energy ◽  
Apparent Digestibility ◽  
Gross Energy

ABSTRACTMicronization followed by grinding of brown-seeded sorghum increased starch availability in vitro but had no effect on proximate composition of the grain. In diets containing 70% of sorghum in ground or micronized and ground form, apparent digestibility of dry matter and nitrogen and nitrogen retention by growing pigs were improved (2·5, 5·0 and 12·2% respectively). Addition to the diet of 0*2 g polyethylene glycol per g crude protein in the sorghum fraction produced similar effects. In the ground cereal diet inclusion of polyethylene glycol increased apparent digestibility of gross energy (3·8%) and improved metabolizable energy content by 5·5%, but i n the micronized and ground cereal diet the additive had no effect.

Nutritional evaluation of wheat 1. Effects of preparation on digestibility of dry matter, energy and nitrogen in pigs

1974 ◽  
Vol 19 (3) ◽  
pp. 359-365 ◽  
Author(s):  
M. Ivan ◽  
L. R. Giles ◽  
A. R. Alimon ◽  
D. J. Farrell
Keyword(s):  
Dry Matter ◽  
Nitrogen Retention ◽  
Metabolizable Energy ◽  
Apparent Digestibility ◽  
Whole Grain ◽  
Whole Wheat ◽  
Gross Energy ◽  
Dietary Components ◽  
Matter Energy ◽  
Plot Design

SUMMARY1. A split-plot design was used to study apparent digestibility of dry matter, gross energy and nitrogen of a whole grain wheat diet and processed (hammermilled, rolled or hammermilled and then steam-pelleted) wheat diets by eight small (33·9 ± 0·1 kg) and eight large (70±1·7 kg) pigs. Metabolizable energy and nitrogen retention were also studied with the small pigs.2. The processed wheat diets were superior to the whole grain wheat diet in all the parameters measured.3. There were no significant differences between the performance of pigs given the differently processed wheat diets.4. Apparent digestibility of dietary components particularly in the whole wheat diet was significantly higher when diets were given to small pigs than when given to large pigs.

scholarly journals Methods for predicting the energy value of pet foods

2009 ◽  
Vol 38 (spe) ◽  
pp. 1-14 ◽  
Author(s):  
Carlos Castrillo ◽  
Marta Hervera ◽  
Maria Dolores Baucells
Keyword(s):  
Near Infrared ◽  
Metabolizable Energy ◽  
Apparent Digestibility ◽  
Crude Fibre ◽  
Near Infrared Reflectance ◽  
Energy Value ◽  
Close Relationship ◽  
Accuracy Of Prediction

The energy value of foods as well as energy requirements of dogs and cats is currently expressed in terms of metabolizable energy (ME). The determination of ME content of foods requires experimental animals and is too expensive and time consuming to be used routinely. Consequently, different indirect methods have been proposed in order to estimate as reliably an accurately as possible the ME content of pet food. This work analyses the main approaches proposed to date to estimate the ME content of foods for cats and dogs. The former method proposed by the NRC estimates the ME content of pet foods from proximal chemical analysis using the modified Atwater factors, assuming constant apparent digestibility coefficients for each analytical fraction. Modified Atwater factors systematically underestimate the ME content of low-fibre foods whereas they overestimate those that are high in fibre. Recently, different equations have been proposed for dogs and cats based in the estimation of apparent digestibility of energy by the crude fibre content, which improve the accuracy of prediction. In any case, whatever the method of analysis used, differences in energy digestibility related with food processing and fibre digestibility are unlikely to be accounted for. A simple in vitro enzymatic method has been recently proposed based in the close relationship that exist between energy digestibility and organic matter disappearance after two consecutive enzymatic (pepsin-pancreatin) incubation of food sample. Nutrient composition and energy value of pet foods can be also accurately and simultaneously predicted using near infrared reflectance spectroscopy (NIRS).

scholarly journals Energy values and protein digestibility of soybean milk by-product in pigs based on in vitro assays

2020 ◽  
Vol 34 (1) ◽  
pp. 73-81
Author(s):  
Jung Yeol Sung ◽  
Bokyung Hong ◽  
Youngeun Song ◽  
Beob Gyun Kim
Keyword(s):  
Protein Content ◽  
Crude Protein ◽  
Protein Digestibility ◽  
Metabolizable Energy ◽  
In Vitro Assays ◽  
Feed Ingredient ◽  
Soybean Milk ◽  
Gross Energy ◽  
Swine Diets

Background: Soybean milk by-product (SMBP) is a potential alternative feed ingredient in swine diets due to its high protein content. However, information on energy and nutritional values of SMBP used as swine feed ingredient is limited. Objective: To estimate energy values and protein digestibility of SMBP in pigs based on in vitro assays. Methods: Four SMBP samples were obtained from 3 soybean milk-producing facilities. In vitro total tract disappearance (IVTTD) and in vitro ileal disappearance (IVID) of dry matter (DM) in the SMBP samples were determined. In vitro ileal disappearance of crude protein was determined by analyzing crude protein content in undigested residues after determining IVID of DM. Digestible and metabolizable energy of SMBP were estimated using gross energy, IVTTD of DM, and prediction equations. Results: Sample 4 had greater IVTTD of DM than that of sample 3 (97.7 vs. 94.4%, p<0.05), whereas IVID of DM in sample 4 was lower compared with sample 1 (53.5 vs. 65.0%, p<0.05). In vitro ileal disappearance of crude protein in sample 2 was greater than that in sample 1 and 3 (92.6 vs. 90.6 and 90.1%; p<0.05). The estimated metabolizable energy of SMBP ranged from 4,311 to 4,619 kcal/kg as-is basis and the value of sample 3 was the least (p<0.05) among SMBP samples. Conclusion: Energy values and protein digestibility should be determined before using SMBP in swine diets.

The Apparent Digestibility of Nutrients and Energy Value to Pigs of Plantains (Musa Sapientum Var. Paradisiaca Linn)

2021 ◽  
Vol 1 (2) ◽  
pp. 184-191
Author(s):  
V. A. Oyenuga ◽  
B. L. Fetuga
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nitrogen Retention ◽  
Basal Diet ◽  
Metabolizable Energy ◽  
Apparent Digestibility ◽  
Crude Fibre ◽  
Large White ◽  
High Dry Matter ◽  
Nitrogen Free Extract

The proximate and mineral constituents of six plantain forms, raw green plantain (RGP), cooked green plantain (CGP), raw ripe plantain (RRP), cooked ripe plantain (CRP), green plantain meal (GPM) and ripe plantain meal (RPM) were determined by chemical analysis. 24 large white barrows, weighing initially, between 40-45 kg., were used to study the apparent digestibility of nutrients, digestible energy (DE), metabolizable energy (ME), metabolizable energy corrected for nitrogen retention (ME(n) and total digestible nutrients when the plantains served as the only source of nutrients. In a second experiment ME and ME(n) were measured for the plantain forms using 28 Large White barrows, with diets in which the plantains replaced 50% of maize in a basal diet. All the plantain forms had low fat, crude fibre and crude protein content but high in nitrogen-free extract, phosphorus, potassium and Iron. Identically high dry matter (D.M.) and nitrogen-free extract (N.F.E.) digestion coefficients were obtained for all the plantain forms except RPM, which had significantly lower values. Digestion coefficients for crude protein (46.9, 53.6, 51.8, 34.3,-32.7 %), crude fibre, (46.6, 49.5, 58.7, 62.6, 56.9, 39.6 %), ether extract (55.9, 56.6, 66.9, 69.2, 54.7 and 34.6 %) for RGP, CGP, RRP, CRP, GPM and RPM respectively were considerably lower than for D.M. and N.F.E. The DE, ME ME(n) and TDN values were comparable for all plantain forms except RPM, which had significantly lower values in most cases. The fresh green plantains and green plantain meals had slightly higher energy values than the ripe plantains, even though the nutrients in the ripe plantains were better digested than in the green forms. The results are discussed in relation to variations in the relative proportions of the simple and more complex carbohydrates during ripening.

scholarly journals Effect of replacing a commercial pelleted calf meal with lucerne leaf-meal on performance of neonatal and transitional Holstein heifer calves

2018 ◽  
Vol 58 (5) ◽  
pp. 834
Author(s):  
Joyce L. Marumo ◽  
Florence V. Nherera-Chokuda ◽  
Jones W. Ng'ambi ◽  
Mukengela C. Muya
Keyword(s):  
Crude Protein ◽  
Rumen Fluid ◽  
Net Energy ◽  
Rumen Degradation ◽  
Leaf Meal ◽  
Large Ruminant ◽  
Gross Energy ◽  
Dietary Treatments ◽  
Phosphorus Levels

A study was conducted to (i) determine in vitro ruminal dry matter (DM) fermentation kinetics, effective rumen degradation of DM (EDDM), (ii) estimate energy fractions supply of lucerne leaf-meal (LLM) and composite diets of LLM with commercial pelleted calf meal and also (iii) determine the effects of substituting commercial pelleted calf meal with LLM on the intake, % ruminal nitrogen balance (RNB) and growth of the neonates (21–42 days old) and transition (43–56 days old) Holstein heifer calves. Forty-eight Holstein heifer calves were randomly assigned to three different dietary treatments in a complete randomised design of: (a) pelleted concentrate (PEL), (b) 65% pelleted concentrate: 35% LLM (P65 L35); (c) and 50% pelleted concentrate: 50% LLM (P50 L50). The study comprised of two experiments: neonatal (Experiment 1) and transition (Experiment 2) phases. Lucerne leaf-meal had gross energy of 16.2 MJ/kg and 25% crude protein DM. PEL diet was high in starch and bound protein compared with other diets. Inclusion of LLM in diets increased calcium levels but tended to decrease phosphorus levels. Calves were weaned at the age of 56 days. The feeds were incubated for 0, 4, 10, 18, 24 and 48 h using a DaisyII incubator. Rumen fluid was obtained from calves <50 days old. Large Ruminant Nutrition System was used to predict %RNB and energy density of the diets during neonatal and transition phases. Higher mean EDDM levels were found with LLM inclusions whereas fractions a, a + b and c did not vary. Neonates on diet C had higher (P < 0.05) daily DM and crude protein intakes, %RNB, total digestible nutrients, net energy at maintenance and net energy at gain during neonatal phase. Performance of calves was similar during the transition phase. LLM should be considered as a concentrate replacement in diets of neonates and calves.

Determination of the yield characteristics and in vitro digestibility of barley forage harvested in different vegetation periods

2016 ◽  
Author(s):  
Mehtap Guney ◽  
Cagri Kale ◽  
Duran Bolat ◽  
Suphi Deniz
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Energy Content ◽  
Vegetation Period ◽  
Metabolizable Energy ◽  
Mature Stage ◽  
In Vitro Digestibility ◽  
Net Energy ◽  
Seed Formation

This study planned to determine the differences among nutrient composition, in vitro digestibility, energy content, digestible dry matter and organic matter yields of barley forage harvested at three different stages of maturity. Each vegetation period (heading stage, seed formation stage and mature stage) was randomly assigned to 5 replication from 1 square meter area and fifteen samples were harvested in total. DM, ADF (p<0.001), and NDF (p<0.05) contents were different in each stages of barley forage. In vitro dry matter (IVDMD), organic matter digestibility (IVOMD), metabolizable energy (ME), and net energy for lactation (NEL) values of samples were determined to be lower than the other two stages at the mature stage (p<0.05). Yield parameters of barley were significantly affected by vegetation period (p<0.001). It can be concluded that all three vegetation period had significantly higher digestibility. Digestible DM, OM and energy yields were higher when harvested at the mature stage of vegetation.

Efficiency of the utilization of the energy of food in piglets, after weaning

1973 ◽  
Vol 81 (2) ◽  
pp. 295-302 ◽  
Author(s):  
G. Burlacu ◽  
G. Băia ◽  
Dumitra Ionilă ◽  
Doina Moisa ◽  
V. Taṣcenco ◽  
...  
Keyword(s):  
G Protein ◽  
Energy Cost ◽  
Metabolizable Energy ◽  
Net Energy ◽  
Crude Fibre ◽  
Large White ◽  
Maintenance Requirement ◽  
Large White Breed ◽  
White Breed ◽  
Gross Energy

SummaryThe efficiency of utilization of the energy of food by weaned pigs of the Large White breed was measured. Three diets based on maize (40–60%) had a metabolizable energy of 75·8±1·2% of the gross energy, and 78·2 ±3·4% of the metabolizable energy was present as net energy.The maintenance requirement of metabolizable energy of pigs weighing 14·3 ± 2·1 kg was 143·6 kcal/kg°0·75/24 h (601 kJ/kg0·75/24 h).The energy cost per g protein and fat synthesized by piglets was estimated to be 7·43 and 12·05 kcal (31·1 and 50·4 kJ) metabolizable energy, respectively.Equations for calculation of metabolizable energy (Yl, kcal) and of the net energy (Y2, kcal), based on digested nutrients, were:Y1 = 4·64×1 + 9·12×2 + 4·25×3'CV = ± 1·2%,Y2 = 3·84×1 + 7·09×2 + 3·28×3'CV = ± 1·4%,where X1 = g protein digested, X2 = g fat digested, X3= g carbohydrate digested (crude fibre × N-free extracts).

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