scholarly journals Nanoencapsulated Essential Oils with Enhanced Antifungal Activity for Potential Application on Agri-Food, Material and Environmental Fields

Antibiotics ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 31
Author(s):  
Magdaléna Kapustová ◽  
Giuseppe Granata ◽  
Edoardo Napoli ◽  
Andrea Puškárová ◽  
Mária Bučková ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Winning Strategy ◽  
Colloidal Suspensions ◽  
Fungal Species ◽  
Natural Environments ◽  
Food Material ◽  
Minimum Fungicidal Concentration ◽  
Fungal Strains

Nanotechnology is a new frontier of this century that finds applications in various fields of science with important effects on our life and on the environment. Nanoencapsulation of bioactive compounds is a promising topic of nanotechnology. The excessive use of synthetic compounds with antifungal activity has led to the selection of resistant fungal species. In this context, the use of plant essential oils (EOs) with antifungal activity encapsulated in ecofriendly nanosystems could be a new and winning strategy to overcome the problem. We prepared nanoencapsules containing the essential oils of Origanum vulgare (OV) and Thymus capitatus (TC) by the nanoprecipitation method. The colloidal suspensions were characterized for size, polydispersity index (PDI), zeta potential, efficiency of encapsulation (EE) and loading capacity (LC). Finally, the essential oil nanosuspensions were assayed against a panel of fourteen fungal strains belonging to the Ascomycota and Basidiomycota phyla. Our results show that the nanosystems containing thyme and oregano essential oils were active against various fungal strains from natural environments and materials. In particular, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values were two to four times lower than the pure essential oils. The aqueous, ecofriendly essential oil nanosuspensions with broad-spectrum antifungal activity could be a valid alternative to synthetic products, finding interesting applications in the agri-food and environmental fields.

scholarly journals Antifungal Activity of Rosmarinus Officinalis Essential Oil and Nystatin on Store Isolate of Candida species from HIV/AIDS Patients with Oral Candidiasis

2020 ◽  
Vol 32 (3) ◽  
pp. 167
Author(s):  
Bernadya Yogatri Anjuwita ◽  
Iskandar Zulkarnain ◽  
Muhammad Yulianto Listiawan ◽  
Evy Ervianti ◽  
Rahmadewi Rahmadewi ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Candida Species ◽  
Oral Candidiasis ◽  
Rosmarinus Officinalis ◽  
Minimum Fungicidal Concentration ◽  
Microdilution Method ◽  
Rosemary Essential Oil

Background: Oral candidiasis is caused by the mycotic activity of Candida albicans present in the oral cavity, and it is one of the most common opportunistic infections found in patients with Human Immunodeficiency Virus (HIV)/acquired immune deficiency syndrome (AIDS). The growing resistance and side effects to common antifungal drugs have promoted herbal essential oils as antifungal agents in recent years. In this study, essential oils (EO) of Rosmarinus officinalis (Lamiaceae) were examined for in vitro antifungal activ­ity against Candida species. Purpose: To evaluate the antifungal activity of essential oils of Rosmarinus officinalis (Lamiaceae) and nystatin using the microdilution technique by determining the minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) of Candida spesies. Methods: This was an experimental laboratory study with a post-test-only design conducted in Dr. Soetomo General Academic Teaching Hospital, Surabaya. Forty isolates consisted of twenty isolates of Candida albicans and twenty isolates of Candida non-albicans were collected. The isolates were tested for antifungal activity using the microdilution on 96-well plates. Result: There was a significant difference from the results of the MIC concentration of rosemary essential 100% to 6.25% microdilution method between nystatin and rosemary essential oil (p < 0.05). Conclusion: The antifungal activity of rosemary essential oil was better than nystatin that the lowest MIC value, which was 6.25%, has been obtained the microdilution method. The minimum fungicidal concentration of rosemary essential oil was 25%, while the minimum fungicidal concentration nystatin was higher than 100%.

scholarly journals Effect of seasonality on chemical profile and antifungal activity of essential oil isolated from leaves Psidium salutare (Kunth) O. Berg

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5476 ◽  
Author(s):  
Delmacia G. de Macêdo ◽  
Marta Maria A. Souza ◽  
Maria Flaviana B. Morais-Braga ◽  
Henrique Douglas M. Coutinho ◽  
Antonia Thassya L. dos Santos ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Fungal Infections ◽  
Fungal Resistance ◽  
Chemical Profile ◽  
Minimum Fungicidal Concentration ◽  
Broth Microdilution Method

Medicinal plants play a crucial role in the search for components that are capable of neutralizing the multiple mechanisms of fungal resistance. Psidium salutare (Kunth) O. Berg is a plant native to Brazil used as both food and traditional medicine to treat diseases and symptoms such as stomach ache and diarrhea, whose symptoms could be related to fungal infections from the genus Candida. The objective of this study was to investigate the influence of seasonal variability on the chemical composition of the Psidium salutare essential oil, its antifungal potential and its effect on the Candida albicans morphogenesis. The essential oils were collected in three different seasonal collection periods and isolated by the hydrodistillation process in a modified Clevenger apparatus with identification of the chemical composition determined by gas chromatography coupled to mass spectrometry (GC/MS). The antifungal assays were performed against Candida strains through the broth microdilution method to determine the minimum fungicidal concentration (MFC). Fungal growth was assessed by optical density reading and the Candida albicans dimorphic effect was evaluated by optical microscopy in microculture chambers. The chemical profile of the essential oils identified 40 substances in the different collection periods with γ-terpinene being the predominant constituent. The antifungal activity revealed an action against the C. albicans, C. krusei and C. tropicalis strains with an IC50 ranging from 345.5 to 2,754.2 µg/mL and a MFC higher than 1,024 µg/mL. When combined with essential oils at sub-inhibitory concentrations (MIC/16), fluconazole had its potentiated effect, i.e. a synergistic effect was observed in the combination of fluconazole with P.salutare oil against all Candida strains; however, for C. albicans, its effect was reinforced by the natural product in all the collection periods. The results show that the Psidium salutare oil affected the dimorphic transition capacity, significantly reducing the formation of hyphae and pseudohyphae in increasing concentrations. The results show that P. salutare oil exhibits a significant antifungal activity against three Candida species and that it can act in synergy with fluconazole. These results support the notion that this plant may have a potential use in pharmaceutical and preservative products.

scholarly journals ANTIFUNGAL POTENTIAL OF Lippia multiflora MOLD. AND Melaleuca leucadendron L. ESSENTIAL OILS AGAINST SOME ROOT BORNE FUNGI OF IPOMEA BATATAS (L) LAM. IN CÔTE D’IVOIRE

2020 ◽  
Vol 8 (5) ◽  
pp. 654-662
Author(s):  
Tia Vama Etienne ◽  
◽  
Cissé Mohamed ◽  
Allani Sophie Aka Christiane ◽  
◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Mycelial Growth ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Composition ◽  
Minimum Concentration ◽  
Minimum Fungicidal Concentration ◽  
Ipomea Batatas ◽  
Aspergillus Sp

The objective of this study was to evaluate the antifungal activity of Lippia multiflor and Melaleuca leucadendron essential oils against three phytopathogenic fungi (Rhizopus sp., Fusarim sp., and Aspergillus sp.) associated with Ipomea batatas tuberous. Essential oils had been extracted by steam distillation method from leaves of selected plants and their chemical composition was characterized by gas chromatography-mass spectrometry (GC-MS). Essential oil composition characterization was followed by the estimation of percentage mycelial growth inhibition, minimum inhibitory concentration, and minimum fungicidal concentration of the isolated essential oils against the tested pathogens by the Sabouraud dilution medium method. Antifungal activity of different concentrations of isolated essential oils was evaluated by using the microdilution method. The most abundant compounds identified in the M. leucadendron essential oil are 1,8-cineole (38.2%), viridiflorol (13.4%), α-Pinene (12.5%), α-terpineol(9.2%), and geranial (22.8%) while L. multiflora essential oil have neral (18.7%), 1,8-Cineole (11.4%), α-Phellandrene (6.8%), sabinene (3.5%) as active ingredients. The concentration of essential oils which completely inhibited the mycelial growth and spore germination of all the tested pathogen varies and it was reported 0.33µL/mL and 0.66µL/mL for L. multiflora respectively while it was reported 24 µL/mL for M. leucadendron, for both effect, seven days of incubation. The minimum concentration of fungicidal essential oil against Aspergillus sp. was 0.33µL/mL for L. multiflora. The findings of the current study confirm the fungicidal properties of the tested essential oils and their potential use in the management of economically important fungal pathogens of Ipomea batatas. Further, these essential oils can be used as possible alternatives to synthetic fungicides.

scholarly journals In vitro antifungal activity of Myrcia ovata essential oils and their major compounds against pathogens of citrus, sweet potato, and coconut

2019 ◽  
Vol 35 (6) ◽  
Author(s):  
Larissa Alves Secundo White ◽  
Arie Fitzgerald Blank ◽  
Paulo Roberto Gagliardi ◽  
Maria de Fátima Arrigoni-Blank ◽  
Daniela Aparecida de Castro Nizio ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Sweet Potato ◽  
Colletotrichum Acutatum ◽  
In Vitro Tests ◽  
Minimum Fungicidal Concentration ◽  
The Individual ◽  
Nerolic Acid

Myrcia ovata, an endemic species to the Brazilian Atlantic Forest, presents antifungal properties. The phytopathogens Colletotrichum acutatum, Plenodomus destruens, and Thielaviopsis paradoxa are responsible for the diseases citrus postbloom fruit drop, sweet potato foot rot, and coconut stem bleeding, respectively. The antifungal activity of the essential oils of five M. ovata chemotypes (MYRO-159, nerolic acid chemotype; MYRO-180, nerolic acid + linalool chemotype; MYRO-388, geraniol chemotype; MYRO-157, citral + (E)-nerolidol chemotype; and MYRO-174, isopulegol + linalool chemotype), four major compounds (nerolic acid, nerolic acid + linalool, geraniol, and citral + (E)-nerolidol), and three pure compounds (citral, (E)-nerolidol, and linalool) against the fungi C. acutatum, P. destruens, and T. paradoxa were evaluated. For this, in vitro tests were conducted in a completely randomized design with three replications, testing concentrations (v/v) ranging from 0.01 to 1.0 μL.mL-1. All treatments presented toxicity at different levels to the three fungi. For C. acutatum, the essential oil from the individual MYRO-180 (nerolic acid + linalool chemotype) and its major compound showed the lowest Minimal Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) of 0.03 and 0.1 µL.mL-1, respectively. For P. destruens, the essential oil from the individual MYRO-159 (nerolic acid chemotype) presented the lowest MIC of 0.05 μL.mL-1. The nerolic acid + linalool chemotype and its major compound presented an MFC of 0.07 μL.mL-1. For T. paradoxa, the major compound citral + (E)-nerolidol stood out with the lowest MIC and MFC of 0.03 and 0.2 µL.mL-1, respectively. Linalool presented the lowest toxicity to the three tested fungi.

scholarly journals Chemical composition and antifungal activity of essential oils from four Asteraceae plants grown in Egypt

2018 ◽  
Vol 73 (7-8) ◽  
pp. 313-318 ◽  
Author(s):  
Rose Vanessa Bandeira Reidel ◽  
Simona Nardoni ◽  
Francesca Mancianti ◽  
Claudia Anedda ◽  
Abd El-Nasser G. El Gendy ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Activity ◽  
First Time

Abstract The objective of the present paper was the assessment of the chemical composition of the essential oils from four Asteraceae species with a considerable food, medicinal, and agricultural value, collected in Egypt, together with their in vitro inhibitory activity against molds and yeasts. The essential oil of Launaea cornuta flowers was also evaluated for the first time, but because of its very low yield (<0.01%), no antifungal test was performed.

scholarly journals Chemical Composition and Antifungal Activity of the Essential Oils of Schinus weinmannifolius Collected in the Spring and Winter

2014 ◽  
Vol 9 (9) ◽  
pp. 1934578X1400900
Author(s):  
Camila Hernandes ◽  
Silvia H. Taleb-Contini ◽  
Ana Carolina D. Bartolomeu ◽  
Bianca W. Bertoni ◽  
Suzelei C. França ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Antimicrobial Action ◽  
Pharmacological Profile

Reports on the chemical and pharmacological profile of the essential oil of Schinus weinmannifolius do not exist, although other Schinus species have been widely investigated for their biological activities. This work aimed to evaluate the chemical composition and antimicrobial activity of the essential oil of S. weinmannifolius collected in the spring and winter. The essential oils were extracted by hydrodistillation, analyzed by GC/MS and submitted to microdilution tests, to determine the minimum inhibitory concentration. The oils displayed different chemical composition and antimicrobial action. Bicyclogermacrene and limonene predominated in the oils extracted in the winter and spring, respectively, whereas only the latter oil exhibited antifungal activity.

scholarly journals Effect of the Chemical Composition of Free-Terpene Hydrocarbons Essential Oils on Antifungal Activity

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3532
Author(s):  
Ben Salha ◽  
Herrera Díaz ◽  
Lengliz ◽  
Abderrabba ◽  
Labidi
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pharmaceutical Products ◽  
Gas Chromatography Mass Spectrometry ◽  
Reduced Pressure ◽  
Oxygenated Compounds ◽  
Food Spoilage Fungi

In this study, Carum carvi L. essential oil (CEO) and Origanum majorana L. essential oil (MEO) was steam-distillated under reduced pressure. We henceforth obtained three fractions for each essential oil: CF1, CF2, CF3, MF1, MF2, and MF3. Then, these fractions were characterized using the gas chromatography–mass spectrometry (GC-MS) technique. The results indicated that some fractions were rich in oxygenated compounds (i.e., CF2, CF3, MF2, and MF3) with concentrations ranging from 79.21% to 98.56%. Therefore, the influence of the chemical composition of the essential oils on their antifungal activity was studied. For this purpose, three food spoilage fungi were isolated, identified, and inoculated in vitro, in order to measure the antifungal activity of CEO, MEO, and their fractions. The results showed that stronger fungi growth inhibitions (FGI) (above 95%) were found in fractions with higher percentages of oxygenated compounds, especially with (−)-carvone and terpin-4-ol as the major components. Firstly, this work reveals that the free-terpenes hydrocarbons fractions obtained from MEO present higher antifungal activity than the raw essential oil against two families of fungi. Then, it suggests that the isolation of (−)-carvone (97.15 ± 5.97%) from CEO via vacuum distillation can be employed successfully to improve antifungal activity by killing fungi (FGI = 100%). This study highlights that separation under reduced pressure is a simple green method to obtain fractions or to isolate compounds with higher biological activity useful for pharmaceutical products or natural additives in formulations.

scholarly journals Antioxidant Activity and Chemical Composition of Essential Oils from Schinus Fasciculata (Griseb.) I.M. Johnst and S. Praecox (Griseb.) Speg

2008 ◽  
Vol 3 (9) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Ana P. Murray ◽  
Silvana A. Rodriguez ◽  
María G. Murray
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenger ◽  
Natural Environments ◽  
Dpph Assay ◽  
Reference Compound ◽  
Fresh Material ◽  
Phenological Phases

Schinus fasciculata and S. praecox (Anacardiaceae) can be found among the characteristic vegetation components of the phytogeographic province of the Espinal. The essential oils from these two species have been obtained from fresh material by hydrodistillation and analyzed by GC and GC-MS. We have compared the chemical composition of the oils obtained from samples collected at two different natural environments and at different phenological phases. The antioxidant activity of these oils was evaluated by the DPPH assay. S. fasciculata and S. praecox showed significant differences in their chemical composition as well as in their radical scavenger ability. The essential oil from samples of S. fasciculata attacked by plant-lice (Hemiptera, Psylloidea) was the more active one, with inhibition percentages higher than that of the reference compound.

scholarly journals Antifungal Activity of Essential Oil ofEucalyptus camaldulensisDehnh. against SelectedFusariumspp.

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Martin Muthee Gakuubi ◽  
Angeline W. Maina ◽  
John M. Wagacha
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Mycelial Growth ◽  
Inhibitory Concentration ◽  
Eucalyptus Camaldulensis ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Minimum Fungicidal Concentration ◽  
Potential Use

The objective of this study was to evaluate the antifungal activity of essential oil (EO) ofEucalyptus camaldulensisDehnh. against fiveFusariumspp. commonly associated with maize.The essential oil had been extracted by steam distillation in a modified Clevenger-type apparatus from leaves ofE. camaldulensisand their chemical composition characterized by gas chromatography mass spectrometry. Poisoned food technique was used to determine the percentage inhibition of mycelial growth, minimum inhibitory concentration, and minimum fungicidal concentration of the EO on the test pathogens. Antifungal activity of different concentrations of the EO was evaluated using disc diffusion method. The most abundant compounds identified in the EO were 1,8-cineole (16.2%),α-pinene (15.6%),α-phellandrene (10.0%), and p-cymene (8.1%). The EO produced complete mycelial growth inhibition in all the test pathogens at a concentration of 7-8 μL/mL after five days of incubation. The minimum inhibitory concentration and minimum fungicidal concentration of the EO on the test fungi were in the range of 7-8 μL/mL and 8–10 μL/mL, respectively. These findings confirm the fungicidal properties ofE. camaldulensisessential oils and their potential use in the management of economically importantFusariumspp. and as possible alternatives to synthetic fungicides.

scholarly journals Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species

2009 ◽  
Vol 58 (11) ◽  
pp. 1454-1462 ◽  
Author(s):  
Eugénia Pinto ◽  
Luís Vale-Silva ◽  
Carlos Cavaleiro ◽  
Lígia Salgueiro
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Cell Membrane ◽  
Fungal Infections ◽  
Yeast Cells ◽  
Syzygium Aromaticum ◽  
Clove Oil ◽  
Fungal Cell ◽  
Minimum Fungicidal Concentration ◽  
Clove Essential Oil

The composition and antifungal activity of clove essential oil (EO), obtained from Syzygium aromaticum, were studied. Clove oil was obtained commercially and analysed by GC and GC-MS. The EO analysed showed a high content of eugenol (85.3 %). MICs, determined according to Clinical and Laboratory Standards Institute protocols, and minimum fungicidal concentration were used to evaluate the antifungal activity of the clove oil and its main component, eugenol, against Candida, Aspergillus and dermatophyte clinical and American Type Culture Collection strains. The EO and eugenol showed inhibitory activity against all the tested strains. To clarify its mechanism of action on yeasts and filamentous fungi, flow cytometric and inhibition of ergosterol synthesis studies were performed. Propidium iodide rapidly penetrated the majority of the yeast cells when the cells were treated with concentrations just over the MICs, meaning that the fungicidal effect resulted from an extensive lesion of the cell membrane. Clove oil and eugenol also caused a considerable reduction in the quantity of ergosterol, a specific fungal cell membrane component. Germ tube formation by Candida albicans was completely or almost completely inhibited by oil and eugenol concentrations below the MIC values. The present study indicates that clove oil and eugenol have considerable antifungal activity against clinically relevant fungi, including fluconazole-resistant strains, deserving further investigation for clinical application in the treatment of fungal infections.

Sign in / Sign up

Export Citation Format

Share Document