scholarly journals Enhanced Antibacterial Potential of Amoxicillin against Helicobacter pylori Mediated by Lactobionic Acid Coated Zn-MOFs

Antibiotics ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1071
Author(s):  
Haseena ◽  
Adnan Khan ◽  
Fariha Aslam ◽  
Tasmina Kanwal ◽  
Muhammad Raza Shah ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Drug Candidate ◽  
Bacterial Cells ◽  
X Ray Diffraction ◽  
Lactobionic Acid ◽  
Atomic Force ◽  
Stomach Adenocarcinoma ◽  
New Perspective ◽  
H Pylori ◽  
Antibacterial Potential

H. pylori (Helicobacter pylori) causes a common chronic infectious disease and infects around 4.4 billion people worldwide. H. pylori was classified as a member of the primary class of stomach cancer (stomach adenocarcinoma). Hence, this study was conducted to design a novel lactobionic acid (LBA)-coated Zn-MOFs to enhance bactericidal activity of Amoxicillin (AMX) against H. pylori. The synthesized Zn-MOFs were characterized by various techniques which included Dynamic Light Scattering (DLS), Fourier Transform Infrared (FT-IR) Spectroscopy, Powder X-ray diffraction, scanning electron microscope, and atomic force microscope. They were capable of encapsulating an increased amount of AMX and investigated for their efficacy to enhance the antibacterial potential of their loaded drug candidate. Interestingly, it was found that LBA-coated Zn-MOFs significantly reduced the IC50, MIC, and MBIC values of AMX against H. pylori. Morphological investigation of treated bacterial cells further authenticated the above results as LBA-coated Zn-MOFs-treated cells underwent complete distortion compared with non-coated AMX loaded Zn-MOFs. Based on the results of the study, it can be suggested that LBA-coated Zn-MOFs may be an effective alternate candidate to provide new perspective for the treatment of H. pylori infections.

scholarly journals Metalloantibiotics: Synthesis, characterization and antimicrobial evaluation of bismuth-fluoroquinolone complexes against Helicobacter pylori

2009 ◽  
Vol 59 (3) ◽  
pp. 259-271 ◽  
Author(s):  
Anwar Shaikh ◽  
Rajani Giridhar ◽  
Francis Megraud ◽  
Mange Yadav
Keyword(s):  
Helicobacter Pylori ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Organometallic Compounds ◽  
Moderate Activity ◽  
Scanning Calorimetry ◽  
Strong Activity ◽  
Antimicrobial Evaluation ◽  
H Pylori ◽  
Antibacterial Potential

Metalloantibiotics: Synthesis, characterization and antimicrobial evaluation of bismuth-fluoroquinolone complexes againstHelicobacter pyloriNovel organometallic compounds have been prepared by complexing the fluoroquinolones, norfloxacin, ofloxacin, ciprofloxacin, sparfloxacin, lomefloxacin, pefloxacin and gatifloxacin, with bismuth. The complexes were characterized by UV, IR, atomic absorption spectroscopy, elemental analysis, differential scanning calorimetry, thermogravimetric analysis and mass spectrometry. Their antibacterial potential againstHelicobacter pyloriand other microorganisms was investigated. These compounds were found to possess strong activity againstHelicobacter pyloriwith a minimum inhibitory concentration of 0.5 mg L-1. They also exhibited moderate activity againstEscherichia coli, Staphylococcus aureus, Bacillus pumilusandStaphylococcus epidermidis.These bismuth-fluoroquinolone complexes have the potential to be developed as drugs againstH. pylorirelated ailments.

Helicobacter pylori-induced Gastritis in Experimentally Infected Conventional Piglets

2001 ◽  
Vol 38 (6) ◽  
pp. 667-678 ◽  
Author(s):  
T. Poutahidis ◽  
T. Tsangaris ◽  
G. Kanakoudis ◽  
I. Vlemmas ◽  
N. Iliadis ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Animal Model ◽  
Helicobacter Pylori ◽  
Human Disease ◽  
Light And Electron Microscopy ◽  
Large Majority ◽  
Bacterial Cells ◽  
New Model ◽  
Pathologic Features ◽  
H Pylori

A conventional nonmutant animal that could be experimentally infected with Helicobacter pylori isolates would be a useful animal model for human H. pylori-associated gastritis. Gnotobiotic and barrier-born pigs are susceptible to H. pylori infection, but attempts to infect conventional pigs with this bacterium have been unsuccessful. In the present study, a litter of eight 20-day-old crossbreed piglets were purchased from a commercial farm. Six of them were orally challenged two to five times at different ages, between 29 and 49 days, with doses of H. pylori inoculum containing approximately 109 bacterial cells. Two animals served as controls. The inoculation program began 2 days postweaning when the piglets were 29 days of age. Prior to every inoculation, the piglets were fasted and pretreated with cimetidine, and prior to the first and second inoculation each piglet also was pretreated with dexamethasone. The challenged piglets were euthanasized between 36 and 76 days of age. H. pylori colonized all six inoculated piglets. The pathology of the experimentally induced gastritis was examined macroscopically and by light and electron microscopy. H. pylori induced a severe lymphocytic gastritis in the conventional piglets and reproduced the large majority of the pathologic features of the human disease. Therefore, the conventional piglet represents a promising new model for study of the various pathogenic mechanisms involved in the development of lesions of the human H. pyloriassociated gastritis.

scholarly journals pH-Regulated Gene Expression of the Gastric Pathogen Helicobacter pylori

2003 ◽  
Vol 71 (6) ◽  
pp. 3529-3539 ◽  
Author(s):  
D. Scott Merrell ◽  
Maria L. Goodrich ◽  
Glen Otto ◽  
Lucy S. Tompkins ◽  
Stanley Falkow
Keyword(s):  
Helicobacter Pylori ◽  
Transcriptional Repression ◽  
Bacterial Genome ◽  
Low Ph ◽  
Acid Exposure ◽  
Protein Accumulation ◽  
Bacterial Cells ◽  
Gastric Pathogen ◽  
H Pylori ◽  
Regulated Gene Expression

ABSTRACT Colonization by the gastric pathogen Helicobacter pylori has been shown to be intricately linked to the development of gastritis, ulcers, and gastric malignancy. Little is known about mechanisms employed by the bacterium that help it adapt to the hostile environment of the human stomach. In an effort to extend our knowledge of these mechanisms, we utilized spotted-DNA microarrays to characterize the response of H. pylori to low pH. Expression of approximately 7% of the bacterial genome was reproducibly altered by shift to low pH. Analysis of the differentially expressed genes led to the discovery that acid exposure leads to profound changes in motility of H. pylori, as a larger percentage of acid-exposed bacterial cells displayed motility and moved at significantly higher speeds. In contrast to previous publications, we found that expression of the bacterial virulence gene cagA was strongly repressed by acid exposure. Furthermore, this transcriptional repression was reflected at the level of protein accumulation in the H. pylori cell.

scholarly journals Helicobacter pylori and Complex Gangliosides

2004 ◽  
Vol 72 (3) ◽  
pp. 1519-1529 ◽  
Author(s):  
Niamh Roche ◽  
Jonas Ångström ◽  
Marina Hurtig ◽  
Thomas Larsson ◽  
Thomas Borén ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Sialic Acid ◽  
Mutant Strain ◽  
Carbohydrate Chain ◽  
Proton Nuclear Magnetic Resonance ◽  
Bacterial Cells ◽  
Wild Type ◽  
Knockout Mutant ◽  
Factors Affecting ◽  
H Pylori

ABSTRACT Recognition of sialic acid-containing glycoconjugates by the human gastric pathogen Helicobacter pylori has been repeatedly demonstrated. To investigate the structural requirements for H. pylori binding to complex gangliosides, a large number of gangliosides were isolated and characterized by mass spectrometry and proton nuclear magnetic resonance. Ganglioside binding of sialic acid-recognizing H. pylori strains (strains J99 and CCUG 17874) and knockout mutant strains with the sialic acid binding adhesin SabA or the NeuAcα3Galβ4GlcNAcβ3Galβ4GlcNAcβ-binding neutrophil-activating protein HPNAP deleted was investigated using the thin-layer chromatogram binding assay. The wild-type bacteria bound to N-acetyllactosamine-based gangliosides with terminal α3-linked NeuAc, while gangliosides with terminal NeuGcα3, NeuAcα6, or NeuAcα8NeuAcα3 were not recognized. The factors affecting binding affinity were identified as (i) the length of the N-acetyllactosamine carbohydrate chain, (ii) the branches of the carbohydrate chain, and (iii) fucose substitution of the N-acetyllactosamine core chain. While the J99/NAP− mutant strain displayed a ganglioside binding pattern identical to that of the parent J99 wild-type strain, no ganglioside binding was obtained with the J99/SabA− mutant strain, demonstrating that the SabA adhesin is the sole factor responsible for the binding of H. pylori bacterial cells to gangliosides.

scholarly journals Galectin-3 Plays an Important Role in Innate Immunity to Gastric Infection by Helicobacter pylori

2016 ◽  
Vol 84 (4) ◽  
pp. 1184-1193 ◽  
Author(s):  
Ah-Mee Park ◽  
Satoru Hagiwara ◽  
Daniel K. Hsu ◽  
Fu-Tong Liu ◽  
Osamu Yoshie
Keyword(s):  
Innate Immunity ◽  
Helicobacter Pylori ◽  
Bacterial Cells ◽  
Mucus Layer ◽  
Iodide Uptake ◽  
Gastric Glands ◽  
Content Type ◽  
Galectin 3 ◽  
H Pylori ◽  
Deficient Mice

We studied the role of galectin-3 (Gal3) in gastric infection byHelicobacter pylori. We first demonstrated that Gal3 was selectively expressed by gastric surface epithelial cells and abundantly secreted into the surface mucus layer. We next inoculatedH. pyloriSydney strain 1 into wild-type (WT) and Gal3-deficient mice using a stomach tube. At 2 weeks postinoculation, the bacterial cells were mostly trapped within the surface mucus layer in WT mice. In sharp contrast, they infiltrated deep into the gastric glands in Gal3-deficient mice. Bacterial loads in the gastric tissues were also much higher in Gal3-deficient mice than in WT mice. At 6 months postinoculation,H. pylorihad successfully colonized within the gastric glands of both WT and Gal3-deficient mice, although the bacterial loads were still higher in the latter. Furthermore, large lymphoid clusters mostly consisting of B cells were frequently observed in the gastric submucosa of Gal3-deficient mice.In vitro, peritoneal macrophages from Gal3-deficient mice were inefficient in killing engulfedH. pylori. Furthermore, recombinant Gal3 not only induced rapid aggregation ofH. pyloribut also exerted a potent bactericidal effect onH. pylorias revealed by propidium iodide uptake and a morphological shift from spiral to coccoid form. However, a minor fraction of bacterial cells, probably transient phase variants of Gal3-binding sugar moieties, escaped killing by Gal3. Collectively, our data demonstrate that Gal3 plays an important role in innate immunity to infection and colonization ofH. pylori.

scholarly journals Antimicrobial susceptibility of H. pylori isolated from suspected patient using stool sample at Malali Hospital and Maternity Clinic Kaduna, Kaduna State – Nigeria

2021 ◽  
Vol 14 (3) ◽  
pp. 122-128
Author(s):  
Rabilu Sani
Keyword(s):  
Helicobacter Pylori ◽  
Stool Sample ◽  
Antimicrobial Susceptibility ◽  
Atmospheric Oxygen ◽  
Bacterial Cells ◽  
Selective Media ◽  
H Pylori ◽  
Phosphate Buffered Saline ◽  
Maternity Clinic ◽  
Environment Condition

The antimicrobial susceptibility of Helicobacter pylori isolated from suspected patient using stool sample at Beijing greatest college of health sciences and technology, Kaduna, demonstration clinic. The analyses were carried out to further understand the intestinal environment condition of the sample sites. As it has been noted that: it is difficult of growing Helicobacter pylori in a broth, hence, no specific enrichment has been proposed. Note: As everything present in the stomach would be found in the stools, hence there is no doubt that Helicobacter pylori can be eliminated via this route and successful culturing of Helicobacter pylori from the stool sample had been achieved. [4]. The stool was diluted to a 20% w/v solution of in phosphate buffered saline (PBS) and the suspension was sieved through a 250 um strainer before plating onto the selective media. The sample was passed through a series of dilution techniques and centrifugation before finally plating on to the deoxycholate citrate agar (selective) and incubates at 37oC under the microacrobic atmospheric oxygen concentration of 7 – 12% (O2) for four (4) days. Across the cultured, the cultured plate there was marked abundant of bacterial cells with some colonies having the characteristics of Helicobacter pylori which later sub-cultured and isolated for antimicrobial test.

scholarly journals Function of Non-coding RNA in Helicobacter pylori-Infected Gastric Cancer

2021 ◽  
Vol 8 ◽  
Author(s):  
Chao Wang ◽  
Yiyang Hu ◽  
Huan Yang ◽  
Sumin Wang ◽  
Bo Zhou ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Tumor Progression ◽  
Digestive System ◽  
Non Coding Rna ◽  
Gastric Cancer Treatment ◽  
Multiple Levels ◽  
New Perspective ◽  
H Pylori ◽  
The Relationship

Gastric cancer is a common malignant tumor of the digestive system. Its occurrence and development are the result of a combination of genetic, environmental, and microbial factors. Helicobacter pylori infection is a chronic infection that is closely related to the occurrence of gastric tumorigenesis. Non-coding RNA has been demonstrated to play a very important role in the organism, exerting a prominent role in the carcinogenesis, proliferation, apoptosis, invasion, metastasis, and chemoresistance of tumor progression. H. pylori infection affects the expression of non-coding RNA at multiple levels such as genetic polymorphisms and signaling pathways, thereby promoting or inhibiting tumor progression or chemoresistance. This paper mainly introduces the relationship between H. pylori-infected gastric cancer and non-coding RNA, providing a new perspective for gastric cancer treatment.

scholarly journals Kinetics and Mechanisms of Extracellular Protein Release by Helicobacter pylori

1999 ◽  
Vol 67 (10) ◽  
pp. 5247-5252 ◽  
Author(s):  
Wayne Schraw ◽  
Mark S. McClain ◽  
Timothy L. Cover
Keyword(s):  
Helicobacter Pylori ◽  
Early Time ◽  
Medium Composition ◽  
Protein Release ◽  
Extracellular Protein ◽  
Broth Culture ◽  
Bacterial Cells ◽  
Gastric Mucus ◽  
Extracellular Release ◽  
H Pylori

ABSTRACT To investigate the kinetics and mechanisms of extracellular protein release by Helicobacter pylori, we analyzed the entry of metabolically radiolabeled bacterial proteins into broth culture supernatant. At early time points, vacuolating cytotoxin (VacA) constituted a major extracellular protein. Subsequently, culture supernatants accumulated many proteins that were components of intact bacterial cells. This nonselective release of proteins was associated with a decreasing turbidity of cultures and loss of bacterial viability, indicative of an autolytic process. The rates of VacA secretion and autolysis were each influenced by medium composition, and therefore these may be regulated phenomena. Extracellular release of proteins by H. pylori may be an important adaptation that facilitates the persistence of H. pylori in the human gastric mucus layer. Moreover, entry of proinflammatory proteins into the gastric mucosa may contribute to the induction of a mucosal inflammatory response.

Biofilm formation and modulation of luxS and rpoD expression by Helicobacter pylori

Biofilms ◽  
2005 ◽  
Vol 2 (2) ◽  
pp. 119-127 ◽  
Author(s):  
L. Cellini ◽  
R. Grande ◽  
T. Traini ◽  
E. Di Campli ◽  
S. Di Bartolomeo ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Environmental Stress ◽  
Biofilm Formation ◽  
Single Cells ◽  
Bacterial Cells ◽  
Dominant Form ◽  
Multicellular Aggregate ◽  
Spherical Cells ◽  
H Pylori ◽  
Biofilm Structure

Bacteria often choose a sessile biofilm lifestyle as a strategy to overcome environmental stress. In this study, we describe biofilm formation by Helicobacter pylori on a polystyrene surface, evaluating the viability and the morphological dynamics of bacterial cells during multicellular aggregate development. Moreover, we investigate expression of the luxS and rpoD genes, which are involved in biofilm formation.Two clinically susceptible and resistant strains of H. pylori were analyzed, as well as H. pylori ATCC 43629 for reference. The dominant form of expression, clustered bacterial cells arranged in an abundant matrix, was recorded after 2 days of incubation without shaking. Coccoid (spherical) cells with a “wrinkled” aspect presented the prevalent morphology (59.26%) among cells in the biofilm structure as observed by scanning electron microscopy. In aged H. pylori cultures, death occurred in single cells or cells grouped into microcolonies in which degenerated bacteria were localized inside the aggregates. The expression of luxS and rpoD genes among the sessile bacterial population reached a maximum after 2 days, with a significant reduction at subsequent time-points. No differences in gene expression and biofilm formation were recorded in the three evaluated strains.The morphological fickleness expressed in the life cycle by H. pylori strains emphasizes the bacterium's ability to overcome environmental stress, balancing its spread both outside and inside the host.

scholarly journals Assignment of Absolute Configurations of Two Promising Anti-Helicobacter pylori Agents from the Marine Sponge-Derived Fungus Aspergillus niger L14

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 5061
Author(s):  
Jia Liu ◽  
Ronglu Yu ◽  
Jia Jia ◽  
Wen Gu ◽  
Huawei Zhang
Keyword(s):  
Helicobacter Pylori ◽  
Aspergillus Niger ◽  
Absolute Configuration ◽  
Marine Sponge ◽  
Positive Control ◽  
Human Pathogens ◽  
X Ray Diffraction ◽  
Inhibitory Effects ◽  
X Ray ◽  
H Pylori

A chemical investigation into endozoic fungus Aspergillus niger L14 derived from the marine sponge of Reniera japonica collected off Xinghai Bay (China) resulted in the isolation of two dimeric naphtho-γ-pyrones, fonsecinone A (1) and isoaurasperone A (2). Through a combination of ECD spectra and X-ray diffraction analysis, the chiral axes of compounds 1 and 2 were unambiguously determined as Rα-configurations. Bioassay results indicated that these substances exhibited remarkably inhibitory effects on human pathogens Helicobacter pylori G27 and 159 with MIC values of ≤4 μg/mL, which are similar to those of the positive control, ampicillin sodium. To the best of our knowledge, this is the first report on absolute configuration of 1 and crystallographic data of 2, as well as their potent anti-H. pylori activities.

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