scholarly journals Evaluation of Antibiotics Residues in Chicken Meat Samples in Lebanon

Antibiotics ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 69 ◽  
Author(s):  
Adla Jammoul ◽  
Nada El Darra
Keyword(s):  
Health Concern ◽  
Penicillin G ◽  
Consumer Health ◽  
Antibiotic Residues ◽  
The European Union ◽  
Beta Lactams ◽  
Chicken Muscles ◽  
Antibiotics Residues ◽  
Meat Samples ◽  
Antibiotic Residue

Antibiotic residue in chicken is a human health concern due to its harmful effects on consumer health. This study aims at screening the antibiotic residues from 80 chicken samples collected from farms located in different regions of Lebanon. An optimized multi-class method for identification and quantification of 30 antibiotics from four different chemical classes (sulfonamides, tetracyclines, quinolones, and beta-lactams) has been developed by using liquid chromatography–mass spectrometry. The evaluation of antibiotics residues in 80 chicken muscles samples has shown that 77.5% of samples were at least contaminated with antibiotics residues, out of which 53.75% were exposed to co-occurrence of multidrug residues. The screening of the four antibiotics families has shown that ciprofloxacin (quinolones) represents the highest occurrence percentage (32.5%), followed by amoxicillin (β-lactams) (22.5%) and then tetracyclines (17.5%). Means of sarafloxacin, amoxicillin, and penicillin G residues levels were above the Maximum Residue Limit (MRL) recommended limit according to the European Union EC. This study revealed that chicken samples collected from Lebanese farms contain antibiotic residues. Guidelines for prudent use of antimicrobials agents for chicken should be adopted to reduce the prevalence of resistant Salmonella in chicken.

Validation of Antibiotic Residue Tests for Dairy Goats

1998 ◽  
Vol 61 (3) ◽  
pp. 344-349 ◽  
Author(s):  
S. S. ZENG ◽  
S. HART ◽  
E. N. ESCOBAR ◽  
K. TESFAI
Keyword(s):  
High Performance ◽  
Goat Milk ◽  
High Sensitivity ◽  
Penicillin G ◽  
Antibiotic Residues ◽  
Test Results ◽  
Beta Lactam ◽  
Dairy Goats ◽  
Test Kit ◽  
Antibiotic Residue

The SNAP test, LacTek test (B-L and CEF), Charm Bacillus sterothermophilus var. calidolactis disk assay (BsDA), and Charm II Tablet Beta-lactam sequential test were validated using antibiotic-fortified and -incurred goat milk following the protocol for test kit validations of the U.S. Food and Drug Administration Center for Veterinary Medicine. SNAP, Charm BsDA, and Charm II Tablet Sequential tests were sensitive and reliable in detecting antibiotic residues in goat milk. All three assays showed greater than 90% sensitivity and specificity at tolerance and detection levels. However, caution should be taken in interpreting test results at detection levels. Because of the high sensitivity of these three tests, false-violative results could be obtained in goat milk containing antibiotic residues below the tolerance level. Goat milk testing positive by these tests must be confirmed using a more sophisticated methodology, such as high-performance liquid chromatography, before the milk is condemned. LacTek B-L test did not detect several antibiotics, including penicillin G, in goat milk at tolerance levels. However, LacTek CEF was excellent in detecting ceftiofur residue in goat milk.

scholarly journals Monitoring of Antibiotic Residues in Chicken Meat, Cow and Buffalo Milk Samples in Nepal

2020 ◽  
Vol 8 (3) ◽  
pp. 355-362
Author(s):  
Tulsi Ram Gompo ◽  
Ramchandra Sapkota ◽  
Manita Subedi ◽  
Pragya Koirala ◽  
Pragya Koirala ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Buffalo Milk ◽  
Chicken Meat ◽  
Antibiotic Residues ◽  
Maximum Residue Limit ◽  
Cross Sectional ◽  
Animal Products ◽  
Milk Samples ◽  
Meat Samples ◽  
Antibiotic Residue

In animal products, antibiotic residue is a serious concern for public health in the Himalayan nation, Nepal. A cross-sectional study on antibiotic residues in chicken meat, milk samples of cows, and buffaloes was conducted in selected districts of different provinces of Nepal. Altogether 126 chicken meat samples, 42 each from Kailali (Farwestern Province), Kaski (Gandaki Province), and Nuwakot (Bagmati Province) districts were tested for tetracycline, sulphonamides, penicillin, gentamicin, streptomycin residues by the ELISA method. At Kaski district approximately 57 % (24/42), 50 % (21/42) and 83% (35/42) of meat samples were tested positive for gentamicin (mean-ppb:11.5 μg/Kg/L), tetracycline (mean-ppb:1.44 μg/Kg/L), and fluoroquinolones (mean-ppb: 11.46 μg/ Kg/L) residues respectively. In Kailali and Nuwakot district, 48% (20/42) and 50 % (21/ 42) of meat samples deemed positive for sulphonamides (mean-ppb:15.9 μg/ Kg/L) and penicillin (mean-ppb: 0.39 μg/ Kg/L) residues, respectively. Of the total 168 tests performed in milk samples from Kavre district, 55% (93/168) of the milk samples were tested positive to antibiotic residues for gentamicin, streptomycin, and sulphonamides. The chicken meat samples had antibiotic residues within the national maximum residue limit (MRL); however, milk samples exceeded the national MRL for sulphonamides residues (mean-ppb: 26.44 μg/ Kg/L). The effective surveillance for antibiotic residues in animal products should be implemented strictly in Nepal. Int. J. Appl. Sci. Biotechnol. Vol 8(3): 355-362

Antibiotic Residues in Chickens and Farmers’ Knowledge of Their Use in Tay Ninh Province, Vietnam, in 2017

2020 ◽  
Vol 32 (2-3) ◽  
pp. 126-132
Author(s):  
Nguyen Thi Huong-Anh ◽  
Dang Van Chinh ◽  
Tran Thi Tuyet-Hanh
Keyword(s):  
Chicken Meat ◽  
Health Concern ◽  
Antibiotic Residues ◽  
Appropriate Use ◽  
Attitude And Practice ◽  
Kap Survey ◽  
Meat Samples ◽  
Harmful Effects ◽  
Knowledge Attitude And Practice ◽  
Drug Sellers

Antibiotic residues in chicken meat samples is a serious public health concern due to their harmful effects on consumers’ health. We conducted this research in 2017 to determine antibiotic residues in chicken meat samples and to describe the knowledge, attitude, and practice (KAP) of farmers on the antibiotic uses in poultry farms at Tay Ninh province. Farmers who raised chickens at their farmhouses were surveyed using a KAP survey questionnaire, and 106 chicken meat samples were analyzed for 29 kinds of antibiotic residues. The results showed that 27.4% samples were positive with 11 kinds of 4 antibiotic groups: fluoroquinolones, sulfonamides, tetracycline, and macrolides. A total of 50.9% farmers needed to improve their knowledge on the appropriate use of antibiotics. Although 54.7% famers had positive attitude, just 17% had good practices. It was recommended that the levels of antibiotic residues in chickens should be communicated to food safety and veterinary authorities, chicken farmers, and the general public. Enhancing the training and coaching for each group of farmers to improve their knowledge and practices on the appropriate use of antibiotics in raising chickens is needed. Local government should enhance the level of control and punishment for drug sellers and end users regarding the use of banned antibiotics in chickens.

scholarly journals Status of Antibiotic Residues in Poultry Meat of Nepal

2018 ◽  
Vol 35 ◽  
pp. 55-62 ◽  
Author(s):  
M. Prajapati ◽  
E. Ranjit ◽  
R. Shrestha ◽  
S.P. Shrestha ◽  
S.K. Adhikari ◽  
...  
Keyword(s):  
Health Sector ◽  
Poultry Meat ◽  
Enzyme Linked Immunosorbent Assay ◽  
Poultry Production ◽  
Antibiotic Residues ◽  
Animal Products ◽  
Consumer Safety ◽  
Livestock Products ◽  
Antibiotics Residues ◽  
Meat Samples

Antibiotic residues in livestock products are the burning issue in animal production as well as public health sector. Imprudent use of antibiotics in poultry production increases the risk of antibiotic resistance thus increasing risk in both animals and humans. To determine the trend of antibiotic residues presence in poultry meat, a study was conducted from June 2016 to May 2017 in Kathmandu, Kaski, and Chitwan districts of Nepal. A total of 92 breast samples of broilers ( Kathmandu-30, Kaski-40 and Chitwan-22) were collected randomly from different selling outlets of the study areas and tested for residues of commonly used antibiotic viz. Enrofloxacin (EX), Ciprofloxacin (CIP), Streptomycin (STR) and Chloramphenicol (CHL) using the Enzyme Linked Immunosorbent Assay (ELISA) technique. Out of 92 samples, 57 (62%) samples were found positive for antibiotics residue of which 38% samples were positive for STR residue, 15.2% for CIP and 8.7% for EX. However, none of the samples showed positivity for CHL residue. The level of STR was determined to be higher in all districts in comparison to other antibiotics. The meat samples from Kaski were found to contain higher level of STR (56.1%) while highest percentage of CIP (16.1%) was detected in Kathmandu. Also, presence of EX was found highest in Kathmandu compared to other districts. The study concluded that antibiotics residues have been abundantly found in poultry meat which can have serious effect in human health. Hence, to mitigate this, routine antibiotics residues monitoring and surveillance programmes in food animal products should be conducted to ensure the consumer safety.

Identification of antibiotics in meat. Flow cytometry capabilities

Vsyo o myase ◽  
2020 ◽  
pp. 56-60
Author(s):  
Zayko E.V. ◽  
◽  
Kuznetsova O.A. ◽  
Bataeva D.S. ◽  
Grudistova M.A. ◽  
...  
Keyword(s):  
Raw Materials ◽  
Animal Husbandry ◽  
Flow Cytometer ◽  
Poultry Meat ◽  
Meat Processing ◽  
Beta Lactams ◽  
Antimicrobial Substances ◽  
Chemotherapy Drugs ◽  
Processing Plants ◽  
Meat Samples

The problem of the uncontrolled use of antibiotics currently remains unresolved. Step-by-step monitoring of meat using modern methods will reduce the risk of using contaminated meat raw materials for food production. Qualitative monitoring will identify samples containing residual antimicrobial substances. The use of methods for identifying groups of antibiotics will help narrow the search for antibiotics by expensive chromatographic methods. A study was carried out of beef, pork and poultry meat, which is used in meat processing plants in the production of raw smoked sausages, using two methods. At the first stage, using a qualitative microbiological method, the raw meat was evaluated for the presence of antimicrobial substances, then their group was determined using a NovoCyte flow cytometer. According to the results of a study on a flow cytometer, it was found that out of 10 groups of antibiotics that can be determined by the tested method, the group of lincosamides was not found in all meat samples. The most common groups of chemotherapy drugs in pork were sulfonamides – 29.6 %, tetracycline group – 18.5 % and beta-lactams – 14.8 %, and in beef aminoglycosides – 36.7 %, phenicols – 30 % and beta-lactams – 13.3 %. In poultry meat samples, the most common were sulfonamides – 23.2 %, fenicols – 23.2 %, and beta-lactams – 16 %. Five groups of antibiotics were found in all studied types of meat: fenicols, β-lactams, macrolides, polypeptide antibiotics, and quinolones. This indicates their widespread use in animal husbandry and poultry farming.

scholarly journals Study on the hydrothermal liquefaction of antibiotic residues with molecular sieve catalysts in the ethanol–water system: focus on product distribution and characterization

RSC Advances ◽  
2021 ◽  
Vol 11 (43) ◽  
pp. 26763-26772
Author(s):  
Jian Yang ◽  
Chen Hong ◽  
Yi Xing ◽  
Zixuan Zheng ◽  
Zaixing Li ◽  
...  
Keyword(s):  
Molecular Sieve ◽  
Water System ◽  
Product Distribution ◽  
Hydrothermal Liquefaction ◽  
Raw Material ◽  
Antibiotic Residues ◽  
Bio Oil ◽  
Antibiotic Residue ◽  
System Focus

In this study, the antibiotic residue was used as a raw material to catalyze hydrothermal liquefaction (HTL) in an ethanol–water system to prepare bio-oil.

Antibiotic-Resistant Bacteria and Antibiotic Residue Contamination in Fresh Raw Foods Sold at Wholesale Markets in Thailand

2021 ◽  
Vol 104 (4) ◽  
pp. 654-662
Keyword(s):  
Bacterial Infection ◽  
Food Samples ◽  
Resistant Bacteria ◽  
Antibiotic Residues ◽  
Wholesale Market ◽  
Antibiotic Resistant Bacteria ◽  
Thai Population ◽  
Antibiotic Resistant ◽  
Raw Foods ◽  
Antibiotic Residue

Objective: To investigate the extent of antibiotic-resistant bacteria and antibiotic residue contamination in fresh raw foods sold at wholesale markets in Thailand, which may be the important drivers of antibiotic-resistant bacteria colonization and antibiotic-resistant bacterial infection in Thai population. Materials and Methods: Fresh raw foods, including food from animal products, seafoods, vegetables, fruits, and honey were purchased from two large wholesale markets in Thailand. Food samples were cultured for antibiotic-resistant bacteria and tested for the presence and amount of antibiotic residue. Results: Among 521 samples for bacterial culture, 86.9% grew at least one kind of bacteria. Enterobacteriaceae were commonly isolated and were commonly resistant to ampicillin (76.7% to 100%). ESBL-producers and ceftriaxone-resistant Enterobacteriaceae were prevalent in swine and duck samples (56.7% to 91.7%). Some isolates were resistant to co-amoxiclav (13.3% to 60.0%) and cefoxitin (5.0% to 30.0%). Colistin-resistant Enterobacteriaceae were observed in pork meat (1.4%) and chicken offal (7.0%). Ertapenem-resistant Enterobacteriaceae were detected in cha-om (26.7%). Among 501 samples for antibiotic residue testing, 37.1% contained at least one antibiotic residue. Enrofloxacin was the most prevalent antibiotic residue, followed by doxycycline and tilmicosin. Although most samples contained less antibiotics than the maximum residue limit (MRL), 7.0% contained an amount of at least one antibiotic above the MRL. Conclusion: Many fresh raw foods sold at wholesale markets in Thailand were contaminated with antibiotic-resistant bacteria, and some contained antibiotic residues. Therefore, Thai people are at risk of being colonized with antibiotic-resistant bacteria and developing antibiotic-resistant bacterial infection due to consuming foods contaminated with antibiotic-resistant bacteria or containing antibiotic residues. Keywords: antibiotic-resistant bacteria, antibiotic residue, fresh raw foods, wholesale market, Thailand

Protecting Consumer Health by Developing an Electrochemical Immunosensor for the Detection of Banned Antibiotic Residues in Honey

2021 ◽  
Vol MA2021-01 (55) ◽  
pp. 1366-1366
Author(s):  
Valerie Gaudin ◽  
Céline Hédou ◽  
Christophe Soumet ◽  
Eric Verdon
Keyword(s):  
Electrochemical Immunosensor ◽  
Consumer Health ◽  
Antibiotic Residues

scholarly journals Could Dampening Expression of theNeisseria gonorrhoeaemtrCDE-Encoded Efflux Pump Be a Strategy To Preserve Currently or Resurrect Formerly Used Antibiotics To Treat Gonorrhea?

mBio ◽  
2019 ◽  
Vol 10 (4) ◽  
Author(s):  
Shaochun Chen ◽  
Kristie L. Connolly ◽  
Corinne Rouquette-Loughlin ◽  
Alexander D’Andrea ◽  
Ann E. Jerse ◽  
...  
Keyword(s):  
Genital Tract ◽  
Efflux Pump ◽  
Health Concern ◽  
Third Generation ◽  
Global Public Health ◽  
Beta Lactam ◽  
Beta Lactams ◽  
Genital Tract Infection ◽  
Infected Female ◽  
Content Type

ABSTRACTNeisseria gonorrhoeaehas developed resistance to every antibiotic introduced for treatment of gonorrhea since 1938, and concern now exists that gonorrheal infections may become refractory to all available antibiotics approved for therapy. The current recommended dual antibiotic treatment regimen of ceftriaxone (CRO) and azithromycin (AZM) is threatened with the emergence of gonococcal strains displaying resistance to one or both of these antibiotics. Non-beta-lactamase resistance to penicillin and third-generation cephalosporins, as well as low-level AZM resistance expressed by gonococci, requires overexpression of themtrCDE-encoded efflux pump, which in wild-type (WT) strains is subject to transcriptional repression by MtrR. Since earlier studies showed that loss of MtrCDE renders gonococci hypersusceptible to beta-lactams and macrolides, we hypothesized that transcriptional dampening ofmtrCDEwould render an otherwise resistant strain susceptible to these antibiotics as assessed by antibiotic susceptibility testing and during experimental infection. In order to test this hypothesis, we ectopically expressed a WT copy of themtrRgene, which encodes the repressor of themtrCDEefflux pump operon, inN. gonorrhoeaestrain H041, the first reported gonococcal strain to cause a third-generation-cephalosporin-resistant infection. We now report that MtrR production can repress the expression ofmtrCDE, increase antimicrobial susceptibilityin vitro, and enhance beta-lactam efficacy in eliminating gonococci as assessed in a female mouse model of lower genital tract infection. We propose that strategies that target the MtrCDE efflux pump should be considered to counteract the increasing problem of antibiotic-resistant gonococci.IMPORTANCEThe emergence of gonococcal strains resistant to past or currently used antibiotics is a global public health concern, given the estimated 78 million infections that occur annually. The dearth of new antibiotics to treat gonorrhea demands that alternative curative strategies be considered to counteract antibiotic resistance expressed by gonococci. Herein, we show that decreased expression of a drug efflux pump that participates in gonococcal resistance to antibiotics can increase gonococcal susceptibility to beta-lactams and macrolides under laboratory conditions, as well as improve antibiotic-mediated clearance of gonococci from the genital tract of experimentally infected female mice.

scholarly journals Antibiotic resistance and residue in chicken, cattle, buffalo and goat meats in different southern districts of Bangladesh

2021 ◽  
Vol 5 (1) ◽  
pp. 19-26
Author(s):  
Bidyut Matubber ◽  
Farzana Islam Rume ◽  
Mohammad Enamul Hoque Kayesh ◽  
Mohammad Mahfuzur Rahman ◽  
Mohammad Rohul Amin ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Chicken Meat ◽  
Human Consumption ◽  
Antibiotic Residues ◽  
Salmonella Spp ◽  
Resistance Patterns ◽  
Cattle Meat ◽  
Meat Samples ◽  
Controlling Measures ◽  
Antibiotic Resistance Patterns

The presence of antibiotic residue in chicken and animal meats is a serious threat to human health due to its harmful effects. This study aimed at identifying the antibiotic resistance patterns of the isolates as well as antibiotic residues in chicken, cattle, buffalo and goat meats in different southern districts of Bangladesh. A total of 205 meat samples, including 70 chicken meat, 60 cattle meat, 50 buffalo meat and 25 goat meat were aseptically collected and analysed for the detection of antibiotic residues by thin layer chromatography and the isolates obtained from these samples were subjected to antibiogram study against 16 commonly used antibiotics. The isolates found in this study were Staphylococcus spp., Streptococcus spp., Escherichia coli, and Salmonella spp. and their prevalence were 37.5% (77/205), 22.1% (48/205), 29.7% (61/205), 8.7% (19/205), respectively. The isolates showed different degrees of sensitivity to the antibiotics used in the study. The most resistant phenotype was against cefradine, amoxicillin, penicillin, oxytetracycline, erythromycin, and enrofloxacin. 18.5% (38/205) meat samples were found to be positive for antibiotic residues and the highest prevalence was observed in chicken meat compared to other meat types. Overall, the findings of the study suggest that it is important to take controlling measures for the emergence of antibiotic resistance and also for ensuring healthy meats for human consumption. Asian Australas. J. Food Saf. Secur. 2021, 5 (1), 19-26

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