scholarly journals Selenium Biofortification Impacts the Nutritive Value, Polyphenolic Content, and Bioactive Constitution of Variable Microgreens Genotypes

Antioxidants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 272 ◽  
Author(s):  
Antonio Pannico ◽  
Christophe El-Nakhel ◽  
Giulia Graziani ◽  
Marios C. Kyriacou ◽  
Maria Giordano ◽  
...  

Selenium (Se) is considered essential for human nutrition as it is involved in the metabolic pathway of selenoproteins and relevant biological functions. Microgreens, defined as tender immature greens, constitute an emerging functional food characterized by overall higher levels of phytonutrients than their mature counterparts. The nutraceutical value of microgreens can be further improved through Se biofortification, delivering Se-enriched foods and potentially an enhanced content of bioactive compounds. The current study defined the effect of sodium selenate applications at three concentrations (0, 8, and 16 μM Se) on the bioactive compounds and mineral content of coriander, green basil, purple basil, and tatsoi microgreens grown in soilless cultivation. Analytical emphasis was dedicated to the identification and quantification of polyphenols by UHPLC-Q-Orbitrap-HRMS, major carotenoids by HPLC-DAD, and macro micro-minerals by ICP-OES. Twenty-seven phenolic compounds were quantified, of which the most abundant were: Chlorogenic acid and rutin in coriander, caffeic acid hexoside and kaempferol-3-O(caffeoyl) sophoroside-7-O-glucoside in tatsoi, and cichoric acid and rosmarinic acid in both green and purple basil. In coriander and tatsoi microgreens, the application of 16 μM Se increased the total phenols content by 21% and 95%, respectively; moreover, it improved the yield by 44% and 18%, respectively. At the same Se dose, the bioactive value of coriander and tatsoi was enhanced by a significant increase in rutin (33%) and kaempferol-3-O(feruloyl)sophoroside-7-O-glucoside (157%), respectively, compared to the control. In green and purple basil microgreens, the 8 μM Se application enhanced the lutein concentration by 7% and 19%, respectively. The same application rate also increased the overall macroelements content by 35% and total polyphenols concentration by 32% but only in the green cultivar. The latter actually had a tripled chicoric acid content compared to the untreated control. All microgreen genotypes exhibited an increase in the Se content in response to the biofortification treatments, thereby satisfying the recommended daily allowance for Se (RDA-Se) from 20% to 133%. The optimal Se dose that guarantees the effectiveness of Se biofortification and improves the content of bioactive compounds was 16 μM in coriander and tatsoi, and 8 μM in green and purple basil.

2021 ◽  
Vol 13 (4) ◽  
pp. 2087
Author(s):  
Maria Isabella Sifola ◽  
Linda Carrino ◽  
Eugenio Cozzolino ◽  
Luisa del Piano ◽  
Giulia Graziani ◽  
...  

Tobacco cultivation is characterized by high amounts of waste biomasses whose disposal frequently represents a complex and expensive problem. A study was conducted to evaluate thepotential of pre-harvest light air-cured (Burley) and dark fire-cured (Kentucky) tobacco waste biomasses as a source of bioactive compounds (nutraceutical ingredients) such as polyphenols. Pre-harvest waste materials (topping fresh materials and residual stalks at final harvest) were collected to determine dry matter, total polyphenols content (TPC; Folin assay), and DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,20-azino-bis(3-ethylbenzothiazoline-60-sulfonic acid) diammonium salt) antioxidant capacity. Polyphenols quali-quantitative profiles obtained by Orbitrap Q Exactive of both tobacco types were also determined. Total pre-harvest waste biomass amounted to 3956.9 and 1304.4 kg d.w. ha−1 in light air-cured (Burley) and dark fire-cured (Kentucky) tobacco types, respectively. Polyphenols content, expressed as g kg−1 dry weight (d.w.), ranged between 4.6 and 15.7 g kg−1 d.w. and was generally greater in leaves than in stalks. Considering both leaves and stalks, the light air-cured (Burley) tobacco crop yielded 22.1 kg ha−1 of polyphenols, while the dark fire-cured (Kentucky) tobacco yielded 12.0 kg ha−1. DPPH and ABTS were significantly greater in leaves than in stalks waste biomass in both types of tobacco. The most abundant components were quinic and chlorogenic acids, rutin, and luteolin rutinoside.


2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 26-27
Author(s):  
Caroline Chappell ◽  
Landon Marks ◽  
Katie Mason ◽  
Mary K Mullenix ◽  
Sandra L Dillard ◽  
...  

Abstract A 2-yr study was conducted at Black Belt Research and Extension Center in Marion Junction, AL, to evaluate the effect of nitrogen (N) fertilizer application rate on forage production characteristics, nutritive value, and animal performance of beef heifers grazing a mixture of native warm-season grasses (NWSG) including big bluestem, little bluestem, and indiangrass. Six, two-hectare plots were randomly assigned to one of two treatments (0 or 67 kg N ha-1 applied in early April; n = 3 replications per treatment). Paddocks were continuously stocked with four weaned Angus × Simmental beef heifers (initial BW 288 ± 7 kg) from late May/early June through mid-to-late August during 2018 (73 grazing d) and 2019 (70 grazing d), respectively. Put-and-take cattle were used to manage forage to a target of 38 cm. Forage mass and canopy heights were collected every two weeks during the trial. Visual ground cover ratings, canopy light interception, and botanical composition were measured at the beginning and end of the trial in each year. Hand-plucked samples were collected every two weeks during the grazing trial to determine forage nutritional value. Data were analyzed using the PROC MIXED procedure in SAS 9.4, and differences were declared significant when P ≤ 0.05. Nitrogen fertilized NWSG had greater crude protein (P < 0.0001), sward heights (P = 0.0003), and canopy light interception at the beginning of the season (P = 0.0049) compared to non-fertilized paddocks. However, there were no differences (P ≥ 0.05) among N-fertility treatments for mean forage mass, heifer ADG, or BCS across the 2-yr study. Botanical composition data indicated that indiangrass decreased from 64% to 61% (P = 0.0022) and weed pressure increased from 11% to 15% (P = 0.0064) across the summer grazing season. Canopy light interception decreased by 51% from early June to August in fertilized NWSG and 26% in unfertilized paddocks, respectively. These data illustrate that NWSG systems may provide a viable grazing system in the summer months under reduced N inputs.


1966 ◽  
Vol 25 (1) ◽  
pp. 52-57 ◽  
Author(s):  
K. M. Barth ◽  
G. W. Vander Noot ◽  
W. S. MacGeath ◽  
E. T. Koenegay

2020 ◽  
Vol 10 (10) ◽  
pp. 3468 ◽  
Author(s):  
Ewelina Hallmann ◽  
Piotr Sabała

The aim of this work was to determine the bioactive compounds concentration in herbs from organic and conventional production. In 2017 and 2018, herbs of four species, including basil (Ocimum basilicum L.), bear’s garlic (Allium ursinum L.), marjoram (Origanum majorana L.), and oregano (Origanum vulgare L.), were examined. The concentrations of polyphenols, phenolic acids, flavonoids, carotenoids, and chlorophylls were measured. Next, separation and identification of the individual bioactive compounds were completed. The obtained results show that organic herbs contained significantly higher concentrations of total polyphenols, flavonoids, and phenolic acids compared to conventional herbs in both investigation years. On the other hand, conventional herbs contained significantly higher concentrations of chlorophylls and carotenoids, including beta-carotene.


2007 ◽  
Vol 58 (9) ◽  
pp. 884 ◽  
Author(s):  
J. L. Jacobs ◽  
G. N. Ward

Dairy farms in southern Australia generally use a 2-pond system to manage dairy shed effluent. This system consists of a deep anaerobic first pond and a shallow aerobic second pond. The liquid in the second pond contains a range of nutrients that may have agronomic benefits for forages. The effect of applying second-pond dairy effluent to a summer turnip (Brassica rapa L.) crop over 3 consecutive summer periods was measured. Effluent was applied at 6 rates, 0, 15, 30, 45, 60, and 75 mm, approximately 6–8 weeks after turnips were sown each year. Turnips were assessed for dry matter (DM) accumulation, nutritive characteristics, and mineral content. In addition, total annual production for years 1 and 2 was calculated by including the DM accumulation from annual ryegrass grown from autumn to spring each year. Concentrations of nutrients within the effluent as an average over the 3 years were 31, 454, 20, and 149 kg/ML for phosphorus (P), potassium (K), sulfur (S), and nitrogen (N), respectively. In addition, effluent also contained 152 kg/ML of calcium (Ca), 225 kg/ML of magnesium (Mg), and 529 kg/ML of sodium (Na). Soil pH was generally unaffected with effluent application, while soil EC and total soluble salt (TSS) content increased with effluent addition. In the first year, application of effluent at 15 mm and higher resulted in increases in available K; however, in subsequent years, rates of 45 mm and higher led to an increase in available K, while for the control and lower effluent rates there was a marked decline in K status. In all years there was a linear increase (P < 0.05) in leaf, root, and total DM yields with applied effluent. For leaf, responses were 19, 50, and 26 kg DM per mm applied effluent and for roots, 10, 39, and 25 kg DM per mm applied effluent for years 1, 2, and 3, respectively. In years 2 and 3, turnip leaf crude protein (CP) content increased (P < 0.05) in a linear manner at rates of 0.046 and 0.044% per mm applied effluent, respectively. There was also a linear increase (P < 0.05) in turnip root CP in years 2 and 3 of 0.033 and 0.021% per mm applied effluent, respectively. In all years there was a linear increase (P < 0.05) in leaf K content, while for root K there was a quadratic trend (P < 0.05) for year 1 and a linear increase (P < 0.05) for years 2 and 3. The results from this study indicate that the use of dairy effluent can increase DM yield and improve the nutritive value of turnips through an increase in CP content. The data also indicate that this effect can be maintained over consecutive years, which in turn may provide greater flexibility for returning effluent to farm land. While results appear to indicate that the primary responses are due to N, further work is required to determine the effects of water and other nutrients within dairy effluent.


2001 ◽  
Vol 41 (2) ◽  
pp. 169 ◽  
Author(s):  
Y. J. Ru ◽  
J. A. Fortune

The nutritive value of 26 cultivars of dry, mature subterranean clover was evaluated at Shenton Park, Perth, Western Australia. The cultivars were divided into 3 maturity groups according to flowering time and each cultivar was sown in blocks comprising 4 replicates. The plots were grazed by sheep at 2-week intervals during the growing season. Dry mature plant material and soil were sampled in summer to examine the effect of grazing and cultivar on seed yield and nutritive value of feed residues. Cultivars heavily grazed in spring had a low herbage mass. There was no difference in seed yield and seed weight between heavily and lightly grazed cultivars. Dry matter digestibility and mineral content of dry residues was inconsistent for the 2 grazing treatments. The dry matter digestibility of dry, mature subterranean clover ranged from 40 to 56%, with a wide range of crude fibre, nitrogen and mineral content for the 26 cultivars. While most minerals in the dry residues were above the requirement for sheep, 7 cultivars had a zinc content less than the maintenance requirement for sheep. There was an imbalance for all cultivars in calcium: phosphorus with a range of 4–10: 1. Concurrent estimates on the yield and composition of seed indicated that seed can be resource of minerals for grazing animals in summer. Most cultivars had a seed yield over 100 g/m2 with that of 9 cultivars being over 130 g/m2. Seed was rich in nitrogen, sodium, phosphorus, potassium, magnesium, zinc and copper, and poor in sodium, calcium and manganese. However, there were no cultivars with an appropriate ratio of calcium and phosphorus. The imbalance in nitrogen and sulfur was a result of high nitrogen content with the ratio ranging from 19: 1 to 29: 1.


2020 ◽  
Vol 75 (1) ◽  
pp. 111-126 ◽  
Author(s):  
Maarit Termonen ◽  
Panu Korhonen ◽  
Sanna Kykkänen ◽  
Anna Kärkönen ◽  
Minna Toivakka ◽  
...  

Agronomy ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 860 ◽  
Author(s):  
Anna Grobelna ◽  
Stanisław Kalisz ◽  
Marek Kieliszek

In this study, we aimed to investigate the effect of processing methods and storage time on selected parameters of quality in the purees of blue honeysuckle berries. We investigated the content of bioactive compounds such as anthocyanins, L-ascorbic acid, and total polyphenols (TPs). We tested four processing methods and two varieties of blue honeysuckle berry (Lonicera caerulea L.var. Sineglaska and Volshebnica). The purees were analyzed immediately after production, and after two and four months of storage at 20 °C without exposure to light. According to our results, thermal treatment of the fruits for 5 min resulted in obtaining purees with a higher content of anthocyanins and TPs, but lower content of L-ascorbic acid. However, sieving fruits that were not thermally treated resulted in a higher content of L-ascorbic acid. Furthermore, purees prepared from the fruits of the Volshebnica cultivar were richer in bioactive compounds than that of purees prepared from the Sineglaska cultivar. In addition, the content of anthocyanins, L-ascorbic acid, and TPs decreased with storage time.


2000 ◽  
Vol 51 (8) ◽  
pp. 1047 ◽  
Author(s):  
Y. J. Ru ◽  
J. A. Fortune

With the decline in pasture quality in southern Australia, the development of management strategies to improve nutrient supply for grazing animals is essential and requires a clear understanding of the interaction between animals and plants. The impact of grazing intensity on the morphology of subterranean clover was previously examined. This paper reports the effect of grazing intensity on the nutritive value of subterranean clover, and the variation in quality of cultivars during the growing season. Grazing intensity influenced nutritive value and interacted with cultivar maturity. Heavy grazing depressed dry matter digestibility (DMD) by 5 percentage units in October for early maturity cultivars but increased DMD by 3 percentage units in September for mid maturity cultivars. The influence of grazing intensity on nitrogen content was small. Heavy grazing did not affect acid detergent fibre for the early maturity group, but depressed it for the mid maturity group throughout the season. Acid detergent lignin remained comparable for all cultivars during the season. Mineral content of subterranean clover showed variable response to grazing treatments. Nutritive value varied among cultivars within each maturity group. DMD ranged over 53–64%, 44–62%, and 45–53% for early, mid, and late maturity groups, respectively, at the end of the growing season. The cultivar rank in all nutritional parameters changed with the progress of the season. The large ranges in the decline rate of DMD within each maturity group during the last 8 weeks of growth gave an indication of the potential quality of the cultivars during late spring and early summer. Despite the variation in mineral content there were no cultivars in which the concentration of minerals was below the minimum requirements of sheep. These results indicate that there is a potential for the selection of high quality cultivars within a breeding program, and that indicative targets of grazing intensity need to be further developed with a focus on pasture quality.


1930 ◽  
Vol 20 (4) ◽  
pp. 587-617 ◽  
Author(s):  
H. E. Woodman ◽  
R. E. Evans

A Notable contribution to our knowledge of the factors which influence the nutritive value of grass has been made by Dr J. B. Orr in a recent publication dealing with minerals in pastures and their relation to animal nutrition (l). The treatise was primarily the outcome of the deliberations of a sub-committee appointed in 1926 by the Civil Research Committee of the Cabinet to consider and report on the relationship between the mineral content of pastures and their nutritive value. From the initial enquiries instituted by this sub-committee, it was evident that malnutrition in cattle and sheep arising from deficiency of minerals in grass was widespread in the pastoral areas of the Empire, and that the subject, which was of great economic significance, warranted close and systematic investigation. A report to this effect was duly forwarded to the Civil Research Committee, on whose further recommendation grants were made by the Empire Marketing Board in aid of a comprehensive scheme of investigations into the mineral aspects of pastures within the Empire. A two-fold scheme of work was adopted, actual investigations in selected grassland areas being supplemented by a detailed search of the literature dealing with every phase of the subject.


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