Effect of a Decellularized Omentum Scaffold with Combination of Mesenchymal Stem Cells and Platelet-Rich Plasma on Healing of Critical-Sized Bone Defect: A Rat Model

The high costs and extensive time needed for the treatment of critical-sized bone defects are still major clinical concerns in orthopedic surgery; therefore, researchers continue to look for more cost and time-effective methods. This study aims to investigate the effects of a decellularized omentum scaffold with a combination of platelet-rich plasma (PRP) and mesenchymal stem cells on the healing of critical-sized bone defects. Wistar albino rats (n = 30) were investigated in five groups. Critical-sized bone defects were formed on bilateral radius shafts. No scaffold, decellularized omentum, omentum with PRP and omentum + mesenchymal stem cells was used in group 1 (control group), 2, 3 and 4, respectively. In addition, omentum with a combination of mesenchymal stem cells +PRP was used in group 5. After 6 weeks, both radiological and histological healing were evaluated comparatively among the groups. After the use of a decellularized omentum scaffold, vitality of new cells was maintained, and new bone formation occurred. When compared to the control group, radiological healing was significantly better (p = 0.047) in the omentum and omentum + PRP-treated groups. Furthermore, histological healing was better in the omentum and omentum + PRP-treated groups than the control group (p = 0.001). The use of a decellularized omentum scaffold is suitable in the healing of critical bone defects.

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Effect of a Decellularized Omentum Scaffold With Combination of Mesenchymal Stem Cells and Platelet Rich Plasma on Healing of Critical-sized Bone Defect

10.21203/rs.3.rs-806042/v1 ◽

2021 ◽

Author(s):

Abdulsamet Emet ◽

Erdi Ozdemir ◽

Duygu Uckan Cetinkaya ◽

Emine Kilic ◽

Ali Celalettin Sinan Yuruker ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Platelet Rich Plasma ◽

Bone Defects ◽

Control Group ◽

Albino Rats ◽

Group 5 ◽

Group 2 ◽

New Research ◽

Critical Bone Defects

Abstract Background: High-cost and the long time needed for the treatment of critical-sized bone defects is still major clinical concern in orthopedic surgery, therefore new research continues to identify more cost and time-effective methods. In this study, we aimed to investigate the effect of a decellularized omentum scaffold with a combination of platelet-rich plasma (PRP) and mesenchymal stem cells on the healing of critical- sized bone defects. Materlials and methods: Wistar albino rats (n=30) were investigated in five groups. Critical-sized bone defects were formed on bilateral radius shafts. To increase healing, no scaffold, decellularized omentum, omentum with with PRP and omentum mesenchymal stem cells was used in group 1 (control group) , 2, 3 and 4 respectively. In addition omentum with a combination of mesenchymal stem cells and PRP was used in group 5. After 6 weeks, both radiological and histological healing were evaluated comparatively among groups. Results: After use of decellularized omentum scaffold, vitality of new cells maintained, new bone formation occurred. When compared to control group, radiological healing was statistically significantly better in omentum and omentum with PRP-treated groups (p=0.047). Furthermore histological healing was better in the omentum and omentum with PRP-treated groups than both control and omentum with mesenchymal stem cell-treated groups according to Salkeld Scoring System (p=0.001).Conclusion: The use of decellularized omentum scaffold is suitable in the healing of critical bone defects.

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Osteoprogenitor cells can enhance early bone formation in critical bone defects in dogs

Ciência Rural ◽

10.1590/0103-8478cr20151109 ◽

2017 ◽

Vol 47 (7) ◽

Author(s):

Endrigo Gabellini Leonel Alves ◽

Rogéria Serakides ◽

Isabel Rodrigues Rosado ◽

Omar Leonardo Aristizabal Paez ◽

Jéssica Alejandra Castro Varon ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Bone Formation ◽

Bone Defects ◽

Autogenous Bone ◽

Control Group ◽

New Bone Formation ◽

Osteoprogenitor Cells ◽

Critical Bone Defects

ABSTRACT: The aim of this study was to evaluate the effect of osteoprogenitor cells derived from mesenchymal stem cells from adipose tissue (OC-AD-MSCs), and differentiated into osteoblasts, in the treatment of critical bone defects in dogs. Adipose tissue derived mesenchymal stem cells (AD-MSCs) were subjected to osteogenic differentiation for 21 days and used in the treatment of bone defects in dogs radius. Either three experimental groups were bone defects treated with OC-AD-MSCs (OC), defects filled with autogenous bone (Control- C +), or empty defects (Control- C -). Bone regeneration was assessed by radiology, densitometry, and histomorphometry. The area of new bone formation was higher in the OC group compared to the control group (C-) on postoperative day 15. Defects treated with OC-AD-MSCs showed greater neovascularization than the other two groups at 90 days. We concluded that treatment with OC-AD-MSCs increased the area of new bone formation 15 days after surgery; however, it didn’t complete the bone union in critical bone defects in the radius of dogs at 90 days.

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The Curative Effect of Berberine Nanoparticles and Cisplatin Combination Therapies Against Hepatocarcinogenesis-Induced By N-Nitroso-Diethylamine In Male Rat

JOURNAL OF ADVANCES IN BIOLOGY ◽

10.24297/jab.v11i1.7207 ◽

2018 ◽

Vol 11 (1) ◽

pp. 2180-2200

Author(s):

Nema Abdelhameed Mohamed ◽

Awatef Mohamed Ali ◽

Doaa Ahmed Ghareeb ◽

Adham Rashed Mohamed ◽

Yasmin Mohamed Elmokhtar

Keyword(s):

Gene Expression ◽

Male Rats ◽

Male Rat ◽

Control Group ◽

Albino Rats ◽

Group 4 ◽

Ccl4 Treatment ◽

Group 5 ◽

Group 2 ◽

Wistar Albino Rats

This study aimed to investigate whether berberine nanoparticles (BBR-NPs) and/or cisplatin supplementation could prevent hepatocarcinogenesis-induced by N-nitroso-diethylamine (DENA) in male rats. Male Wistar albino rats were divided into five groups; Group 1: Control; Group 2: DENA-CCl4; Group 3: DENA-CCl4+Cisplatin; Group 4: DENA-CCl4+BBR-NPs; Group 5: DENA-CCl4+Cisplatin+BBR-NPs. DENA-CCl4 significantly increase AST, ALT, ALP, LDH, GGT, AFP activities and total bilirubin, while, 5, NT, total protein and albumin decreased. DENA-CCl4 treatment caused increment in MDA levels and reduction in SOD, CAT, GPx and GSH in liver tissues. Moreover, DENA-CCl4 increase the gene expression of ADAM17 and TNF-Î± however, P53 was declined. In addition, DENA-CCl4 caused severe histopathological lesions in the liver tissue. Interestingly, administration of berberine nanoparticles alone or in combination with cisplatin improves the hepatocarcinogenesis induced by DENA-CCl4 on the physiological, biochemical, molecular and histological levels by decreasing oxidative stress and preserving gene expression of ADAM17, TNF-Î± and P53. The present findings suggest that BBR-NPs with cisplatin might offer a promising strategy for the prevention of liver cancer.

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Triazophos induced neuro-splenic toxicity and evaluation of antioxidative potential of aqueous Broccoli extract in Wistar albino rats

Journal of Applied and Natural Science ◽

10.31018/jans.v13i2.2644 ◽

2021 ◽

Vol 13 (2) ◽

pp. 616-626

Author(s):

Dharmender Sharma ◽

Gurinder Kaur Sangha

Keyword(s):

Histological Study ◽

Control Group ◽

Albino Rats ◽

White Pulp ◽

Protective Effects ◽

Glutathione S Transferase ◽

Group 4 ◽

Group 5 ◽

Group 2 ◽

Wistar Albino Rats

The present investigation was carried out to assess the antioxidative potential of Broccoli sprouts aqueous extract (BE) against triazophos (TZ) induced oxidative stress (OS) in brain and spleen. In the experimental setup, six groups of rats were formed; Control (group 1), BE (group 2), TZ (group 3), and also BE+TZ groups such as BE1 (group 4), BE2 (group 5) and BE3 (group 6) groups. Rats were orally intubated for 30 days as per experimental design. After sacrifice, OS biomarkers viz; catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and lipid peroxidation (LPO) levels were determined in brain and spleen. Acetylcholinesterase (AChE) activity was observed in plasma and brain samples. Histological study of the spleen in TZ rats showed increased thickness of capsule, congestion and hypocellularity in follicles of spleen’s white pulp and the histoarchitecture was restored in TZ+BE group rats. TZ caused degenerative changes in brain histology and rats showed mild congestion along with haemorrhage in the cerebral cortex. Results suggest that TZ exposure is associated with neural toxicity along with altered spleen stress biomarkers, which further corroborates with histopathological findings. It is inferred that BE exerts multi-mechanistic protective effects against TZ induced neuro-splenic toxicity which is attributable to its protective antioxidant actions.

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Effect of Transplantation of Bone Marrow Derived Mesenchymal Stem Cells and Platelets Rich Plasma on Experimental Model of Radiation Induced Oral Mucosal Injury in Albino Rats

International Journal of Dentistry ◽

10.1155/2017/8634540 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 5

Author(s):

Basma Elsaadany ◽

Samar El Kholy ◽

Dalia El Rouby ◽

Laila Rashed ◽

Tarek Shouman

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Platelet Rich Plasma ◽

Synergetic Effect ◽

Neck Region ◽

Control Group ◽

Albino Rats ◽

Histological Structure ◽

Apoptotic Activity

Normal tissue damage following radiotherapy is still a major problem in cancer treatment. Therefore, the current work aimed at exploring the possible role of systemically injected bone marrow derived mesenchymal stem cells (BM-MSCs) and/or locally injected platelet rich plasma (PRP) in ameliorating the side effects of ionizing radiation on the rat’s tongue. Twelve rats served as control group (N) and 48 rats received a single radiation dose of 13 Gy to the head and neck region; then, they were equally divided into 4 experimental groups: irradiated only (C), irradiated + MSCs (S), irradiated + (PRP) (P), and combined group (PS). Animal scarification occurred in 3 and 7 days after radiation. Then, tongues were dissected and examined histologically and for expression of bcl-2 by RT-PCR. Histological examination of the treated groups (S), (P), and (PS) revealed an obvious improvement in the histological structure of the tongue, compared to group (C), in addition to upregulated expression of bcl-2, indicating decreased apoptotic activity.Conclusion. BM-MSCs and PRP have shown positive effect in minimizing the epithelial atrophy of normal oral mucosa after regional radiotherapy, which was emphasized by decreasing apoptotic activity in these tissues. Nevertheless, combined use of BM-MSCs and PRP did not reveal the assumed synergetic effect in oral tissue protection.

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Enhanced regeneration of bone defects using sintered porous Ti6Al4V scaffolds incorporated with mesenchymal stem cells and platelet-rich plasma

RSC Advances ◽

10.1039/d0ra10215f ◽

2021 ◽

Vol 11 (9) ◽

pp. 5128-5138

Author(s):

Ji Li ◽

Ketao Wang ◽

Xiaowei Bai ◽

Qi Wang ◽

Ningyu Lv ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Regeneration ◽

Platelet Rich Plasma ◽

Bone Defects ◽

Regeneration Of Bone

Porous Ti6AI4V scaffolds incorporated with MSC and PRP are more effective in enhancing the bone regeneration.

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Effects of erdosteine on cyclosporin-A-induced nephrotoxicity

Human & Experimental Toxicology ◽

10.1177/0960327111417907 ◽

2011 ◽

Vol 31 (6) ◽

pp. 565-573 ◽

Cited By ~ 13

Author(s):

M Tutanc ◽

V Arica ◽

N Yılmaz ◽

A Nacar ◽

I Zararsiz ◽

...

Keyword(s):

Oxidative Stress ◽

Cyclosporin A ◽

Protective Role ◽

Control Group ◽

Albino Rats ◽

Protective Mechanism ◽

Antioxidant Parameters ◽

Group 2 ◽

Wistar Albino Rats

Aim: In cyclosporin-A (CsA)-induced toxicity, oxidative stress has been implicated as a potential responsible mechanism. Therefore, we aimed to investigate the protective role of erdosteine against CsA-induced nephrotoxicity in terms of tissue oxidant/antioxidant parameters and light microscopy in rats. Materials and methods: Wistar albino rats were randomly separated into four groups. Group 1 rats treated with sodium chloride served as the control, group 2 rats were treated with CsA, group 3 with CsA plus erdosteine, and group 4 with erdosteine alone. Animals were killed and blood samples were analyzed for blood urea nitrogen (BUN), serum creatinine (Cr), uric acid (UA), total protein (TP), and albumin (ALB) levels. Kidney sections were analyzed for malondialdehyde (MDA) and nitric oxide (NO) levels and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities, as well as for histopathological changes. Results: In the CsA group, MDA, GSH-Px, BUN, and Cr levels were increased. The TP and ALB levels were decreased. These changes had been improved by erdosteine administration. Other biochemical parameters did not show any significant change. Conclusion: These results indicate that erdosteine produces a protective mechanism against CsA-induced nephrotoxicity and suggest a role of oxidative stress in pathogenesis.

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The effect of mesenchymal stem cells, demineralized bone graft and platelet-rich plasma on osteogenesis in rat tibia defects

International Dental Research ◽

10.5577/intdentres.2021.vol11.suppl1.8 ◽

2021 ◽

Vol 11 (Suppl. 1) ◽

pp. 47-55

Author(s):

Zozan Erdoğmuş ◽

Belgin Gülsün

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Graft ◽

Platelet Rich Plasma ◽

Female Rats ◽

Control Group ◽

Osteoblastic Activity ◽

Group I ◽

Rat Tibia ◽

Demineralized Bone

Aim: Deformities of the jaw and face are often caused by infection, inflammation, and cystic and neoplastic pathological conditions. Defects with various aetiologies should be repaired promptly using the most appropriate approach to reconstruct the anatomical form. To treat defects, bone grafts with various combinations have been used. In particular, combinations including cellular products to enhance osteogenic properties have been implemented. In this study, we aimed to investigate the effects of different materials and cells on bone defects by using mesenchymal stem cells (MSCs), which are thought to have a positive effect on healing, demineralized bone graft (DMB) and platelet-rich plasma (PRP). Methodology: We used 55 female rats weighing between 200-250 g, four of which were used to obtain platelet-rich plasma. The remaining animals were divided into five groups. Group I (n = 6) was the operative control group, Group II (n = 24) was given DMB, Group III (n = 24) was given DMB+PRP, Group IV (n = 24) was given MSC+DBG and Group V (n = 24) was given DMB+PRP+MSC applied to rat tibial defects (10 mm x 3 mm x 2 mm). Results: Statistically significant differences were observed in bone osteoblastic activity in tibia defects among the groups (p<0.05). Conclusion: Bone regeneration was significantly improved in groups where MSCs were used in combination with DMB and PRP. How to cite this article: Erdoğmuş Z, Gülsün B. The effect of mesenchymal stem cells, demıneralızed bone graft and platelet-rıch plasma on osteogenesıs ın rat tıbıa defects. Int Dent Res 2021;11(Suppl.1):47-55. https://doi.org/10.5577/intdentres.2021.vol11.suppl1.8 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

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Can N-Acetylcysteine Preserve Peritoneal Function and Morphology in Encapsulating Peritoneal Sclerosis?

Peritoneal Dialysis International ◽

10.1177/089686080902902s41 ◽

2009 ◽

Vol 29 (2_suppl) ◽

pp. 202-205 ◽

Cited By ~ 8

Author(s):

Devrim Bozkurt ◽

Ender Hur ◽

Burcu Ulkuden ◽

Murat Sezak ◽

Hasim Nar ◽

...

Keyword(s):

Peritoneal Dialysis ◽

Cell Count ◽

Degradation Products ◽

Isotonic Saline ◽

Control Group ◽

Albino Rats ◽

Peritoneal Fibrosis ◽

Beneficial Effects ◽

Group 2 ◽

Wistar Albino Rats

Long-term use of the peritoneum as a dialysis membrane results in progressive irreversible dysfunction, described as peritoneal fibrosis. Oxidative stress during peritoneal dialysis has been established in many studies. Generation of reactive oxygen species (ROS) by conventional peritoneal dialysis solutions, regardless of whether produced by high glucose, angiotensin II, or glucose degradation products may be responsible for progressive membrane dysfunction. The well-known antioxidant molecule N-acetylcysteine (NAC) is capable of direct scavenging of ROS. The aim of the present study was to investigate the effect of NAC therapy on both progression and regression of encapsulating peritoneal sclerosis (EPS). We divided 49 nonuremic Wistar albino rats into four groups: Control group—2 mL isotonic saline intraperitoneally (IP) daily for 3 weeks; CG group—2 mL/200 g 0.1% chlorhexidine gluconate (CG) and 15% ethanol dissolved in saline injected IP daily for a total of 3 weeks; Resting group—CG (weeks 1 – 3), plus peritoneal resting (weeks 4 – 6); NAC-R group—CG (weeks 1 – 3), plus 2 g/L NAC (weeks 4 – 6). At the end of the experiment, all rats underwent a 1-hour peritoneal equilibration test with 25 mL 3.86% PD solution. Dialysate-to-plasma ratio (D/P) urea, dialysate white blood cell count (per cubic milliliter), ultrafiltration (UF) volume, and morphology changes of parietal peritoneum were examined. The CG group progressed to encapsulating peritoneal sclerosis, characterized by loss of UF, increased peritoneal thickness, inflammation, and ultimately, development of fibrosis. Resting produced advantages only in dialysate cell count; with regard to vascularity and dialysate cell count, NAC was more effective than was peritoneal rest. Interestingly, we observed no beneficial effects of NAC on fibrosis. That finding may be a result of our experimental severe peritoneal injury model. However, decreased inflammation and vascularity with NAC therapy were promising results in regard to membrane protection.

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A Preliminary Study Comparing Microfracture and Local Adherent Transplantation of Autologous Adipose-Derived Stem Cells Followed by Intraarticular Injection of Platelet-Rich Plasma for the Treatment of Chondral Defects in Rabbits

Cartilage ◽

10.1177/1947603517713816 ◽

2017 ◽

Vol 9 (4) ◽

pp. 410-416 ◽

Cited By ~ 3

Author(s):

Timea Spakova ◽

Judita Amrichova ◽

Jana Plsikova ◽

Denisa Harvanova ◽

Slavomir Hornak ◽

...

Keyword(s):

Stem Cells ◽

Cartilage Defect ◽

Platelet Rich Plasma ◽

Femoral Condyle ◽

Rabbit Model ◽

Intraarticular Injection ◽

Control Group ◽

Adipose Derived Stem Cells ◽

Group 2 ◽

Group 1

Objective This study aimed to compare microfracture and application of adipose-derived stem cells (ADSCs) by local adherent technique enhanced by platelet-rich plasma (PRP) to provide a new approach for the repair of cartilage defect. Design Full-thickness cylindrical defects were created in the medial femoral condyle in 9 New Zealand White rabbits (5 months old, 4.65 ± 0.20 kg). Two groups of rabbits ( n = 3) were either treated with ADSCs (Group 1) or the microfracture technique (Group 2) following intraarticular injection of PRP 3 times in weekly intervals. Rabbits in control group ( n = 3) remained untreated. The outcome was assessed macroscopically, histologically, and immunohistochemically. Results At the end of week 12, Group 1 showed better defect filling compared with Group 2. Specimens treated with the combination of ADSCs and PRP exhibited significant differences from the other groups in all criteria of International Cartilage Repair Society macroscopic scoring system. Conclusions Intraarticular injection of autologous PRP in combination with transplantation of autologous ADSCs by local adherent technique enhances the quality of cartilage defect repair with better results in comparison with microfracture surgery in a rabbit model.

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