scholarly journals Food Sensing: Detection of Bacillus cereus Spores in Dairy Products

Biosensors ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 15 ◽  
Author(s):  
Jasmina Vidic ◽  
Carole Chaix ◽  
Marisa Manzano ◽  
Marc Heyndrickx
Keyword(s):  
Bacillus Cereus ◽  
Dairy Products ◽  
Food Samples ◽  
Detection Methods ◽  
Economic Losses ◽  
Processing Plant ◽  
Advantages And Disadvantages ◽  
Microbiological Methods ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Milk is a source of essential nutrients for infants and adults, and its production has increased worldwide over the past years. Despite developments in the dairy industry, premature spoilage of milk due to the contamination by Bacillus cereus continues to be a problem and causes considerable economic losses. B. cereus is ubiquitously present in nature and can contaminate milk through a variety of means from the farm to the processing plant, during transport or distribution. There is a need to detect and quantify spores directly in food samples, because B. cereus might be present in food only in the sporulated form. Traditional microbiological detection methods used in dairy industries to detect spores show limits of time (they are time consuming), efficiency and sensitivity. The low level of B. cereus spores in milk implies that highly sensitive detection methods should be applied for dairy products screening for spore contamination. This review describes the advantages and disadvantages of classical microbiological methods used to detect B. cereus spores in milk and milk products, related to novel methods based on molecular biology, biosensors and nanotechnology.

scholarly journals The Potential Use of Isothermal Amplification Assays for In-Field Diagnostics of Plant Pathogens

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2424
Author(s):  
Aleksandr V. Ivanov ◽  
Irina V. Safenkova ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Molecular Diagnostics ◽  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Isothermal Amplification ◽  
Detection Methods ◽  
Nucleic Acid Detection ◽  
Advantages And Disadvantages ◽  
Fungal Phytopathogens ◽  
Highly Sensitive Detection ◽  
And Control

Rapid, sensitive, and timely diagnostics are essential for protecting plants from pathogens. Commonly, PCR techniques are used in laboratories for highly sensitive detection of DNA/RNA from viral, viroid, bacterial, and fungal pathogens of plants. However, using PCR-based methods for in-field diagnostics is a challenge and sometimes nearly impossible. With the advent of isothermal amplification methods, which provide amplification of nucleic acids at a certain temperature and do not require thermocyclic equipment, going beyond the laboratory has become a reality for molecular diagnostics. The amplification stage ceases to be limited by time and instruments. Challenges to solve involve finding suitable approaches for rapid and user-friendly plant preparation and detection of amplicons after amplification. Here, we summarize approaches for in-field diagnostics of phytopathogens based on different types of isothermal amplification and discuss their advantages and disadvantages. In this review, we consider a combination of isothermal amplification methods with extraction and detection methods compatible with in-field phytodiagnostics. Molecular diagnostics in out-of-lab conditions are of particular importance for protecting against viral, bacterial, and fungal phytopathogens in order to quickly prevent and control the spread of disease. We believe that the development of rapid, sensitive, and equipment-free nucleic acid detection methods is the future of phytodiagnostics, and its benefits are already visible.

A novel NIR fluorescent probe for highly sensitive detection of HNO and its application in bioimaging

2021 ◽  
Author(s):  
shenwei he ◽  
Jianming Zhu ◽  
peiyao xie ◽  
Jianfei Liu ◽  
Di Zhang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Reactive Nitrogen Species ◽  
Physiological Process ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Reactive Nitrogen ◽  
Nitrogen Species ◽  
Efficient Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Nitroxyl (HNO) as an important reactive nitrogen species (RNS), which plays an important role in multiple physiological process. Therefore, it is urgent to exploit reliable and efficient detection methods for...

scholarly journals Prospects for rapid bioluminescent detection methods in the food industry – a review

2011 ◽  
Vol 23 (No. 3) ◽  
pp. 85-92 ◽  
Author(s):  
P. Dostálek ◽  
T. Brányik
Keyword(s):  
Historical Development ◽  
Food Industry ◽  
Food Samples ◽  
Detection Methods ◽  
Rapid Methods ◽  
Detection Techniques ◽  
Atp Assay ◽  
Advantages And Disadvantages ◽  
Bacterial Bioluminescence ◽  
Fluorescent Method

This review surveys rapid bioluminescent detection techniques applied in food industry and discusses the historical development of the rapid methods. These techniques are divided into two groups: methods based on bioluminescent adenosine triphosphate (ATP) assay, and on bacterial bioluminescence. The advantages and disadvantages of these methods are described. The article provides the bibliography of fluorescent method applications in food samples.    

A Novel Immunoassay Test System for Detection of Modified Allergen Residues Present in Almond-, Cashew-, Coconut-, Hazelnut-, and Soy-Based Nondairy Beverages

2016 ◽  
Vol 79 (9) ◽  
pp. 1572-1582 ◽  
Author(s):  
JONGKIT MASIRI ◽  
LORA BENOIT ◽  
MAHZAD MESHGI ◽  
JEFFREY DAY ◽  
CESAR NADALA ◽  
...  
Keyword(s):  
Polyclonal Antibodies ◽  
Rapid Test ◽  
Test System ◽  
Food Samples ◽  
Cross Reactivity ◽  
Detection Methods ◽  
Robust Detection ◽  
Allergen Detection ◽  
Highly Sensitive ◽  
Milk Substitutes

ABSTRACT A growing number of plant-based milk substitutes have become commercially available, providing an array of options for consumers with dietary restrictions. Though several of these products rival cow's milk in terms of their nutritional profiles, beverages prepared with soy and tree nuts can be a significant concern to consumers because of potential contamination with food allergens. Adding to this concern is the fact that allergen residues from plant-based beverages are modified during manufacturing, thereby decreasing the sensitivity of antibody-based detection methods. Consequently, many commercially available allergen detection kits are less effective for allergens derived from nondairy milk substitutes. To address this limitation, we developed a panel of polyclonal antibodies directed against the modified proteins present in almond, cashew, coconut, hazelnut, and soy milks and incorporated them into rapid lateral flow immunoassay tests configured in both sandwich and competitive format. The tests had robust detection capabilities when used with a panel of various brand-name products, with a sensitivity of 1 ppm and selectivity values of 3 to 5 ppm in nondairy beverages. Minimal cross-reactivity to extracts prepared from common commodities was observed. The development of a highly sensitive and rapid test specifically designed to detect trace quantities of highly modified allergen residues in plant-based, dairy-free beverages will aid food manufacturers and regulatory agencies in monitoring products for these modified allergens when testing environmental and food samples.

Isothermal Target and Probe Amplification Assay for the Real-Time Rapid Detection of Staphylococcus aureus

2015 ◽  
Vol 78 (4) ◽  
pp. 723-727 ◽  
Author(s):  
HYEWON SHIN ◽  
MINHWAN KIM ◽  
EUNJU YOON ◽  
GYOUNGWON KANG ◽  
SEUNGYU KIM ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Rapid Detection ◽  
Food Poisoning ◽  
Detection Methods ◽  
Economic Losses ◽  
Fluorescence Detector ◽  
The Real ◽  
Robust Detection ◽  
Advantages And Disadvantages

Staphylococcus aureus, the species most commonly associated with staphylococcal food poisoning, is one of the most prevalent causes of foodborne disease in Korea and other parts of the world, with much damage inflicted to the health of individuals and economic losses estimated at $120 million. To reduce food poisoning outbreaks by implementing prevention methods, rapid detection of S. aureus in foods is essential. Various types of detection methods for S. aureus are available. Although each method has advantages and disadvantages, high levels of sensitivity and specificity are key aspects of a robust detection method. Here, we describe a novel real-time isothermal target and probe amplification (iTPA) method that allows the rapid and simultaneous amplification of target DNA (the S. aureus nuc gene) and a fluorescence resonance energy transfer–based signal probe under isothermal conditions at 61°C or detection of S. aureus in real time. The assay was able to specifically detect all 91 S. aureus strains tested without nonspecific detection of 51 non–S. aureus strains. The real-time iTPA assay detected S. aureus at an initial level of 101 CFU in overnight cultures of preenriched food samples (kiwi dressing, soybean milk, and custard cream). The advantage of this detection system is that it does not require a thermal cycler, reducing the cost of the real-time PCR and its footprint. Combined with a miniaturized fluorescence detector, this system can be developed into a simplified quantitative hand-held real-time device, which is often required. The iTPA assay was highly reliable and therefore may be used as a rapid and sensitive means of identifying S. aureus in foods.

scholarly journals Biosensors for Deoxynivalenol and Zearalenone Determination in Feed Quality Control

Toxins ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 499
Author(s):  
Krisztina Majer-Baranyi ◽  
Nóra Adányi ◽  
András Székács
Keyword(s):  
Sensitive Detection ◽  
Farm Animals ◽  
Detection Methods ◽  
Sample Treatment ◽  
Minimal Sample ◽  
Current Trends ◽  
Highly Sensitive ◽  
Feed Analysis ◽  
Highly Sensitive Detection ◽  
Analytical Tools

Mycotoxin contamination of cereals used for feed can cause intoxication, especially in farm animals; therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current trends in food/feed analysis are focusing on the application of biosensor technologies that offer fast and highly selective and sensitive detection with minimal sample treatment and reagents required. The article presents an overview of the recent progress of the development of biosensors for deoxynivalenol and zearalenone determination in cereals and feed. Novel biosensitive materials and highly sensitive detection methods applied for the sensors and the application of these sensors to food/feed products, the limit, and the time of detection are discussed.

scholarly journals Nanozyme-Participated Biosensing of Pesticides and Cholinesterases: A Critical Review

Biosensors ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 382
Author(s):  
Hengjia Zhu ◽  
Peng Liu ◽  
Lizhang Xu ◽  
Xin Li ◽  
Panwang Hu ◽  
...  
Keyword(s):  
Environmental Remediation ◽  
Low Cost ◽  
Catalytic Performance ◽  
Agricultural Products ◽  
Detection Methods ◽  
Highly Sensitive ◽  
Potential Applications ◽  
Highly Sensitive Detection ◽  
Excellent Stability

To improve the output and quality of agricultural products, pesticides are globally utilized as an efficient tool to protect crops from insects. However, given that most pesticides used are difficult to decompose, they inevitably remain in agricultural products and are further enriched into food chains and ecosystems, posing great threats to human health and the environment. Thus, developing efficient methods and tools to monitor pesticide residues and related biomarkers (acetylcholinesterase and butylcholinesterase) became quite significant. With the advantages of excellent stability, tailorable catalytic performance, low cost, and easy mass production, nanomaterials with enzyme-like properties (nanozymes) are extensively utilized in fields ranging from biomedicine to environmental remediation. Especially, with the catalytic nature to offer amplified signals for highly sensitive detection, nanozymes were finding potential applications in the sensing of various analytes, including pesticides and their biomarkers. To highlight the progress in this field, here the sensing principles of pesticides and cholinesterases based on nanozyme catalysis are definitively summarized, and emerging detection methods and technologies with the participation of nanozymes are critically discussed. Importantly, typical examples are introduced to reveal the promising use of nanozymes. Also, some challenges in the field and future trends are proposed, with the hope of inspiring more efforts to advance nanozyme-involved sensors for pesticides and cholinesterases.

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