The Potential of Colonic Tumor Tissue Fusobacterium nucleatum to Predict Staging and Its Interplay with Oral Abundance in Colon Cancer Patients

Background. Intestinal microbiota dysbiosis may enhance the carcinogenicity of colon cancer (CC) by the proliferation and differentiation of epithelial cells. Oral Fusobacterium nucleatum (Fn) and Porphyromonas gingivalis (Pg) have the ability to invade the gut epithelium, promoting tumor progression. The aim of the study was to assess whether the abundance of these odontopathogenic bacteria was associated with colon cancer. We also investigated how lifestyle factors could influence the oral Fn and Pg abundance and CC. Methods. Thirty-six CC patients were included in the study to assess the Pg and Fn oral and colon tissue abundance by qPCR. Oral health data, food habits and lifestyles were also recorded. Results. Patients had a greater quantity of Fn in the oral cavity than matched CC and adjacent non-neoplastic mucosa (adj t) tissues (p = 0.004 and p < 0.001). Instead, Pg was not significantly detected in colonic tissues. There was an association between the Fn quantity in the oral and CC tissue and a statistically significant relation between the Fn abundance in adenocarcinoma (ADK) and staging (p = 0.016). The statistical analysis revealed a tendency towards a greater Fn quantity in CC (p = 0.073, η2p = 0.12) for high-meat consumers. Conclusion. In our study, Pg was absent in colon tissues but was correlated with the oral inflammation gingival and plaque indices. For the first time, there was evidence that the Fn oral concentration can influence colon tissue concentrations and predict CC prognosis.

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Cluster mir-17–92 is differentially expressed in colon cancer patients and embryonic colon tissue and may contribute to carcinogenesis through E2F1 expression

Journal of Clinical Oncology ◽

10.1200/jco.2007.25.18_suppl.10525 ◽

2007 ◽

Vol 25 (18_suppl) ◽

pp. 10525-10525

Author(s):

J. Garcia-Foncillas ◽

A. Navarro ◽

E. Bandres ◽

R. Artells ◽

I. Moreno ◽

...

Keyword(s):

Colon Cancer ◽

Embryonic Development ◽

Mirna Expression ◽

Target Genes ◽

Tumor Tissue ◽

Stage I ◽

Differentially Expressed ◽

Embryonic Tissue ◽

Colon Tissue ◽

E2f1 Expression

10525 Background: Mature microRNAs (miRNA) are small RNA molecules that act as negative regulators of gene expression, either inhibiting mRNA by blocking its translation into protein or destroying it by RNA interference. Many miRNAs participate in essential processes, including normal embryonic development and carcinogenesis. Methods: We have assessed 156 mature miRNAs in colon tissue from eleven 7–12-week human embryos and 44 colorectal human samples. Data were analyzed using TIGR Multiexperiment viewer. Two multivariate permutation test were performed. Potential target genes of differentially expressed miRNAs are evaluated by western blot. Results: 28 miRNAs were expressed in stage I tumor tissue but not in the corresponding normal tissue, and 13 of these 28 miRNAs (46%) were also expressed in embryonic tissue. Sixty-four miRNAs were differentially expressed in stage II tumor tissue, and 29 of these 64 miRNAs (45%) were also expressed in embryonic tissue. Some miRNAs that are active during embryogenesis, such as mir-17–92, miR-181a, miR-181b and miR-181c (linked to HOXA11), and miR-10a (linked to HOXB8)23, are also expressed during tumor growth. The analysis of 156 miRNAs by K-means support revealed two well-differentiated groups: the embryos of 7–8 weeks and those of 9–12 weeks. Lower miRNA expression was also observed in tumor tissue from stage I in comparison with stage II disease (P=0.014). Analysis of potential target genes revealed that cluster mir-17–92 is differentially expressed in colon cancer and embryonic tissue and may contribute to carcinogenesis through E2F1 expression. Conclusions: Our findings indicate that miRNAs expressed during the embryonic development of the human colon are also expressed in colon tumor tissue. During colon organogenesis, miRNA expression is at first high, while cells are still undifferentiated, but expression levels decrease as cells become more differentiated. In contrast, during the development of colorectal cancer, this process is reversed. No significant financial relationships to disclose.

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Adenokarsinoma Kolon: Laporan Kasus

e-CliniC ◽

10.35790/ecl.v8i2.30539 ◽

2020 ◽

Vol 8 (2) ◽

Author(s):

Mersy S. Padang ◽

Luciana Rotty

Keyword(s):

Colorectal Cancer ◽

Colon Cancer ◽

Cancer Colorectal ◽

Tumor Tissue ◽

Internal Hemorrhoid ◽

Pathological Examination ◽

Colon Tissue ◽

Extensive Resection ◽

Bowel Movements ◽

Total Cancer

Abstract: Colorectal cancer (CRC) is a malignancy originated from the colon tissue which starts in the large intestine and rectum. In 2018, colorectal cancer was ranked fourth of the total malignancy in Indonesia with the number of cases of 30,017 (8.6% of the total cancer cases in Indonesia). We reported a case of colon cancer (adenocarcinoma) in a female aged 34 years. Diagnosis was based on anamnesis of the presence of liquid bowel movements along with blood, intermittent abdominal pain, and change of defecation pattern. Physical examination obtained tenderness in epigastric and hypochondriac right button regions. Colonoscopy was performed and revealed a colonic tumor suspicious of adenocarcinoma, suspected chronic colitis, and internal hemorrhoid. Pathological examination of the tumor tissue resulted in colonic adenocarcinoma (moderate differentiation). The management of this patient was extensive resection with anastomosis and was planned for adjuvant therapy.Keywords: adenocarcinoma colon cancer, colorectal cancer Abstrak: Kanker kolorektal (KKR) adalah keganasan yang berasal dari jaringan usus besar, yang menyerang usus besar dan rektum. Di Indonesia pada tahun 2018, kanker kolorektal menduduki posisi keempat dari keseluruhan diagnosis kanker dengan jumlah kasus 30.017 (8,6% dari seluruh kasus kanker di Indonesia). Kami melaporkan sebuah kasus kanker kolon (adenokarsinoma) pada seorang perempuan usia 34 tahun. Diagnosis ditegakkan berdasarkan anamnesis adanya buang air besar cair diserta darah, nyeri perut hilang timbul, perubahan pola defekasi. Pada pemeriksaan fisik didapatkan nyeri tekan epigastrium dan nyeri tekan bagian hipocondria kanan. Pada pemeriksaan penunjang dilakukan kolonoskopi dan didapatkan hasil tumor kolon curiga adenocarcinoma, kolitis kronik curiga kolitis ulseratif, dan hemoroid interna. Hasil patologi anatomi dari kolonoskopi yaitu kolitis kronis dengan displasia kolon ascenden dan hasil patologi anatomi jaringan tumor berupa adenokarsinoma kolon (diferensiasi sedang). Tatalaksana yang diberikan untuk pada pasien ini berupa tindakan reseksi luas dengan anastomosis dan direncanakan untuk terapi adjuvan.Kata kunci: adenokarsinoma kolon, kanker kolorektal

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Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

Analytical Methods ◽

10.1039/d1ay00020a ◽

2021 ◽

Author(s):

Lijuan Liu ◽

Shengting Zhang ◽

Xiaodan Zheng ◽

Hongmei Li ◽

Qi Chen ◽

...

Keyword(s):

Carbon Dots ◽

Fusobacterium Nucleatum ◽

Living Cells ◽

First Time ◽

Fluorescent Carbon Dots ◽

Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

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The Assessment of IL-21 and IL-22 at the mRNA Level in Tumor Tissue and Protein Concentration in Serum and Peritoneal Fluid in Patients with Ovarian Cancer

Journal of Clinical Medicine ◽

10.3390/jcm10143058 ◽

2021 ◽

Vol 10 (14) ◽

pp. 3058

Author(s):

Aleksandra Mielczarek-Palacz ◽

Celina Kruszniewska-Rajs ◽

Marta Smycz-Kubańska ◽

Jarosław Strzelczyk ◽

Wojciech Szanecki ◽

...

Keyword(s):

Ovarian Cancer ◽

Peritoneal Fluid ◽

Tumor Tissue ◽

Mrna Level ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Expression Of Genes ◽

Genes Encoding ◽

First Time ◽

Ovarian Serous Cancer

The aim of the analysis was for the first time to assess the expression of genes encoding IL-21 and IL-22 at the mRNA level in ovarian tumor specimens and the concentration of these parameters in serum and peritoneal fluid in patients with ovarian serous cancer. The levels of IL-21 and IL-22 transcripts were evaluated with the use of the real-time RT-qPCR. Enzyme-linked immunosorbent assay (ELISA) was used to determine the concentration of proteins. Quantitative analysis of IL-21 gene mRNA in the tumor tissue showed the highest activity in the G1 degree of histopathological differentiation and was higher in G1 compared to the control group. The concentration of IL-21 and IL-22 in the serum and in the peritoneal fluid of women with ovarian cancer varied depending on the degree of histopathological differentiation of the cancer and showed statistical variability compared to controls. The conducted studies have shown that the local and systemic changes in the immune system involving IL-21 and IL-22 indicate the participation of these parameters in the pathogenesis of ovarian cancer, and modulation in the IL-21/IL-22 system may prove useful in the development of new diagnostic and therapeutic strategies used in patients, which require further research.

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Concurrent measurements of carcinoembryonic antigen, glucosephosphate isomerase, gamma-glutamyltransferase, and lactate dehydrogenase in malignant, normal adult, and fetal colon tissues.

Clinical Chemistry ◽

10.1093/clinchem/26.13.1809 ◽

1980 ◽

Vol 26 (13) ◽

pp. 1809-1812 ◽

Cited By ~ 10

Author(s):

D D Munjal

Keyword(s):

Colon Cancer ◽

Lactate Dehydrogenase ◽

Carcinoembryonic Antigen ◽

Tumor Tissue ◽

Human Colon ◽

Normal Adult ◽

Metastatic Colon Cancer ◽

Glucosephosphate Isomerase ◽

Adult Human ◽

Fetal Organs

Abstract Carcinoembryonic antigen and activities of glucosephosphate isomerase (EC 5.3.1.9), γ-glutamyltransferase (EC 2.3.2.2), and lactate dehydrogenase (EC 1.1.1.27) were measured in aqueous extracts of fetal, normal adult, and malignant human colon tissues. Fetal colon, as well as primary and metastatic colon tumor tissue, showed higher activities of these analytes than did normal adult human colon. Liver metastases of colon cancer gave the highest values, normal adult human colon the lowest. Statistically, these differences were more striking in the case of carcinoembryonic antigen and glucosephosphate isomerase than for γ-glutamyltransferase or lactate dehydrogenase. In contrast to the other markers, γ-glutamyltransferase activity was lower in fetal organs than in normal adult colon and colon tumors. These results are consistent with earlier observations that activities of these markers are significantly increased in the blood of patients with metastatic colon cancer.

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Fr568 FUSOBACTERIUM NUCLEATUM INFECTION IS FACILITATED IN COLON CANCER CELLS EXPRESSING GEL-FORMING MUCINS

Gastroenterology ◽

10.1016/s0016-5085(21)01589-4 ◽

2021 ◽

Vol 160 (6) ◽

pp. S-367

Author(s):

Koki Takeda ◽

Minoru Koi ◽

Jovan Pierre-Charles ◽

Chan Choi ◽

Eric Martens ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Fusobacterium Nucleatum ◽

Colon Cancer Cells

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Silencing long noncoding RNA colon cancer-associated transcript-1 upregulates microRNA-34a-5p to promote proliferation and differentiation of osteoblasts in osteoporosis

Cancer Gene Therapy ◽

10.1038/s41417-020-00264-7 ◽

2021 ◽

Author(s):

Fangke Hu ◽

Chengying Jiang ◽

Guoyun Bu ◽

Yiru Fu ◽

Yanfang Yu

Keyword(s):

Colon Cancer ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Proliferation And Differentiation

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Methylation profile of colon cancer genes in colorectal precursor lesions and tumor tissue: perspectives for screening

Scandinavian Journal of Gastroenterology ◽

10.1080/00365521.2021.1922744 ◽

2021 ◽

pp. 1-9

Author(s):

Thais Sobanski ◽

Lidia Maria Rebolho Batista Arantes ◽

Wellington dos Santos ◽

Marcus Matsushita ◽

Marco Antonio de Oliveira ◽

...

Keyword(s):

Colon Cancer ◽

Tumor Tissue ◽

Methylation Profile ◽

Precursor Lesions ◽

Cancer Genes

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Comments on the updated recommendations of the American College of Rheumatology (ACR) for the treatment of gout. Lifestyle (part 2)

Modern Rheumatology Journal ◽

10.14412/1996-7012-2021-2-83-88 ◽

2021 ◽

Vol 15 (2) ◽

pp. 83-88

Author(s):

M. S. Eliseev

Keyword(s):

Body Weight ◽

Food Habits ◽

The Other ◽

Obese Patients ◽

American College Of Rheumatology ◽

The Russian Federation ◽

The One ◽

Effective Drugs ◽

First Time

Updated ACR recommendations for the treatment of gout concerning lifestyle are discussed. Factors related to a lifestyle, above all food habits, for many years were of leading importance in the treatment of patients with gout, even after application of effective drugs. The authors of the updated ACR recommendations for the first time offered to reconsider the role of environmental factors in the genesis of gout and objectively assess the possibility of its non-drug treatment. On the one hand, regardless of the activity of the disease, the need for restrictions of the alcohol, purine-rich products and fructose-containing beverages, as well as the decrease of body weight in obese patients and vitamin C usage unviability are confirmed. On the other hand, these recommendations are conditional. Their new version of ACR recommendations is significantly different from both its previous version and other international and national recommendations, including recommendations on the diagnosis and treatment of gout used in the Russian Federation.

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