Live Fluorescence Imaging of F-Actin Organization in Chick Whole Embryo Cultures Using Sir-Actin
Keyword(s):
Morphogenesis is a continuous process of pattern formation so complex that it requires in vivo monitoring for better understanding. Changes in tissue shape are initiated at the cellular level, where dynamic intracellular F-actin networks determine the shape and motility of cells, influence differentiation and cytokinesis and mediate mechanical signaling. Here, we stain F-actin with the fluorogenic probe SiR-actin for live fluorescence imaging of whole chick embryos. We found that 50 nM SiR-actin in the culture medium is a safe and effective concentration for this purpose, as it provides high labeling density without inducing morphological malformations.
2016 ◽
Vol 117
(11)
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pp. 2533-2537
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1971 ◽
Vol 29
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pp. 548-549
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1994 ◽
Vol 52
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pp. 378-379
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2020 ◽
Vol 26
(22)
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pp. 2610-2619
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