scholarly journals Evaluation of some cellular and mitochondrial parameters in HT1080 cell line in response to the Ginger extract and one of its components: 6-shogaol

2020 ◽  
Author(s):  
Ludis Morales ◽  
Angie Romero
Keyword(s):  
Cell Line ◽  
Ht1080 Cell ◽  
Ginger Extract

HETEROGENOUS EXPRESSION OF PLASMINOGEN ACTIVATOR (PA) GENES IN THE HUMAN SARCOMA CELL LINE HT1080

1987 ◽  
Author(s):  
Walter E Laug
Keyword(s):  
Gene Expression ◽  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cell Line ◽  
Tissue Type ◽  
Gene Probe ◽  
Ht1080 Cell ◽  
Simultaneous Production ◽  
Tissue Invasion ◽  
Ht1080 Cells

Tumor cell derived PA activities are of crucial importance for tissue invasion and destruction by tumor cells. Therefore, we studied the expression of the PA genes in HT1080 cells using immunoenzymatic methods and specific PA gene probes.Immunenzymatic methods allowed only for the detection of urokinase like PA (u-PA) activities in HT1080 cells which was suppressed by treatment of the cells with dexamethasone (10-7 m). Despite the lack of u-PA activities, the cells still degraded extracellular tissue glycoproteins. Northern blot analysis with specific PA gene probe showed that HT1080 cells express both tissue type PA (t-PA) and u-PA. The enzymatic activities of t-PA were most likely masked by the simultaneous production of inhibitors of PA (PAI). Treatment of HT1080 cells with dexamethasone resulted in increased transcription of t-PA and decreased expression of the u-PA gene, explaining the unchanged tissue destruction by dexamethasone treated HT1080 cells.Cell clones secreting either large or small amounts of enzymatic PA activities were isolated from the parental HT1080 cell line using a fibrin agarose overlay technique.The expression of the u-PA gene was enhanced in high secreting PA clones compared to low secreting PA clones when analyzed on Northern blots. This heterogenous expression of the u-PA gene within the HT1080 cell line was confirmed by in situ hybridization with a specific u-PA gene probe.These findings demonstrate that PA gene expression can be missed with immunenzymatic methods due to simultaneous production of inhibitors of PA. In addition our results show that the expression of a given PA gene may be heterogenous on the cellular level within an established tumor cell line. These findings, therefore, suggest cellular variation of PA gene expression in tumor which may be of fundamental importance for tissue invasion and metastasis by cancer cells.

Radiosensitization and cell cycle phases distribution induced by paclitaxel in human fibrosarcoma HT1080 cell line in vitro

2008 ◽  
Vol 6 (9) ◽  
pp. 59
Author(s):  
R. Moleron ◽  
J. Romero ◽  
I. Zapata ◽  
A. De la Torre ◽  
M.J. Citores ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
Ht1080 Cell ◽  
Cell Cycle Phases

scholarly journals CORRELATION OF ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY OF AMLA AND GINGER

2018 ◽  
Vol 11 (8) ◽  
pp. 263
Author(s):  
Safeena Kulsum ◽  
Amritha Suresh ◽  
Alka Mehta
Keyword(s):  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Cell Line ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Antiproliferative Activity ◽  
Colony Formation ◽  
Inhibitory Effect ◽  
Resistant Cell ◽  
Ginger Extract

Objective: Our study focused on evaluating the anticancer property of ascorbic acid and aqueous extract of amla and ginger.Methods: Antioxidant capacity of ascorbic acid, aqueous extract of amla, and ginger was obtained by 2,2-diphenyl-1-picrylhydrazyl method and total antioxidant capacity (TAC). Vero cell line, PA-1, Cal-27, Cal-27 CisR, and DysMSCTR16 cell lines were treated with antioxidants to evaluate its antiproliferative property using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Colony and spheroid formation assays were also carried out in the presence of extracts to assess its role in anticancer stem cell activity.Results: Two volumes of amla 5 μl (~0.016 g) and 25 μl (~0.08 g) and ginger 5 μl (~0.02 g) and 25 μl (~0.1 g) showed TAC activity equivalent to 0.25 mM and 2 mM ascorbic acid, respectively. Amla and ascorbic acid showed significant antiproliferative property in normal (Vero) p=0.05, cancer (PA-1, Cal-27) p=0.005, and resistant (Cal-27 CisR) p=0.05 cell lines and ginger extract in Vero and Cal-27 cell lines (p=0.05). In PA-1 and Cal-27 CisR cell line, ginger extract showed proliferative activity (p=0.005). Antioxidants showed no antiproliferative activity in DysMSCTR16 cells. Amla extract and ascorbic acid showed significant inhibitory effect on spheroid (p=0.005) and colony formation capacity (p=0.0005) among dysplastic, cancer, and resistant cell lines. Ginger showed inhibitory effect (p=0.05) only in colony formation capacity.Conclusion: Overall, we found a strong correlation between antioxidant and antiproliferative activity of ascorbic acid, amla, and ginger. Amla and ascorbic acid proved to be effective in controlling cell proliferation and self-renewal properties of cancer cells. However, ginger was found to have selective and less antiproliferative effect in comparison to amla.

scholarly journals The Inhibitory Effect of Ginger Extract on Ovarian Cancer Cell Line; Application of Systems Biology

2017 ◽  
Vol 7 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Roghiyeh Pashaei-Asl ◽  
Fatima Pashaei-Asl ◽  
Parvin Mostafa Gharabaghi ◽  
Khodadad Khodadadi ◽  
Mansour Ebrahimi ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Systems Biology ◽  
Cell Line ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Ovarian Cancer Cell Line ◽  
Cancer Cell Line ◽  
Inhibitory Effect ◽  
Ginger Extract

Structural requirements for alpha 1 beta 1 and alpha 2 beta 1 integrin mediated cell adhesion to collagen V

1996 ◽  
Vol 109 (7) ◽  
pp. 1865-1874 ◽  
Author(s):  
F. Ruggiero ◽  
J. Comte ◽  
C. Cabanas ◽  
R. Garrone
Keyword(s):  
Cell Adhesion ◽  
Cell Line ◽  
Binding Sites ◽  
Triple Helix ◽  
Helical Conformation ◽  
Epithelial Cell Line ◽  
Ht1080 Cell ◽  
Native Collagen ◽  
Beta 1 Integrin ◽  
Hbl100 Cell

A large variety of cells adhere to and spread on specific regions within the triple helix of collagens, mainly via alpha 1 beta 1 and alpha 2 beta 1 integrins. Disruption of collagen triple helical integrity generally affects the efficiency of cell adhesion on different collagens including collagen V. This report addresses the question of the importance of the linear sequence of the constitutive alpha-chains versus the triple helical conformation in the recognition of collagen V binding sites. To investigate this question, in vitro renaturation of the isolated alpha 1 (V) and alpha 2 (V) chains was performed according to the annealing procedure and formation of the triple helix was monitored by rotary shadowing and by mild trypsin digestion followed by electrophoretic analysis. The results indicate that the alpha 1 (V) and alpha 2 (V) homotrimeric reassociation can occur up to a full-length triple helix but intermediate forms of 50–200 nm long rod-like segments are also observed. We have previously shown that alpha 1 beta 1 and alpha 2 beta 1 integrins, the major collagen receptors, are also involved in cell adhesion to native collagen V. Therefore we chose the following two different cell lines for this study: HT1080 (a human fibrosarcoma cell line) expressing alpha 2 beta 1 and HBL100 (a human mammary epithelial cell line) containing significant amounts of alpha 1 beta 1 and alpha 2 beta 1 integrins. We showed that both alpha 1 (V) and alpha 2(V) homotrimers induced cell adhesion but refolded alpha2(V) chains were more efficient and promoted cell adhesion as well as native collagen V. Thermal stability of refolded alpha-chains was monitored by adhesion promoting activity and showed that cell adhesion was dependent on triple helical conformation of the substrates. Adhesion in all cases was strongly Mg2+ and Mn(2+)-dependent and Ca2+ ions alone were ineffective. Antibodies against alpha 2 and beta 1 integrin subunits completely inhibited HT1080 cell adhesion to all substrates. Moreover, addition of cyclic RGD peptides, which had been shown to interact with alpha 2 beta 1, dramatically affected HT1080 cell adhesion to native collagen V and to the refolded alpha-chains. Antibody to beta 1 subunits abolished HBL100 cell adhesion to all substrates. A complete inhibition of HBL100 cell adhesion to native collagen V was achieved only by simultaneous addition of function-blocking specific monoclonal antibodies against alpha 1 and alpha 2 integrin subunits. However, only alpha 2 beta 1 was engaged obviously in HBL100 cell adhesion to refolded alpha-chains. These data indicate that triple helical conformation is particularly critical for alpha 2 beta 1- and alpha 1 beta 1-dependent adhesion and that the integrin alpha 2 beta 1 is a dominant functional receptor for refolded alpha-chains. We conclude that alpha 2 beta 1-dependent adhesion seems to involve multiple different conformational binding sites while alpha 1 beta 1-dependent adhesion is more restricted to the heterotrimeric native form of the molecule.

scholarly journals Effect of bioactive compounds in ginger extract on cancer cell lines In vitro.

2012 ◽  
Vol 6 (2) ◽  
pp. 34-41
Author(s):  
Mohammed S. Mohammed Al-Zobaidi ◽  
Muayad S. Shawkat ◽  
Abd Al-Amer N. Galoob
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Exposure Time ◽  
Crude Extract ◽  
Cytotoxic Effect ◽  
Ethanolic Extract ◽  
Malignant Cell ◽  
Mammary Adenocarcinoma ◽  
Ginger Extract

his study was designed to detect the active compounds found in ethanolic crude extract of Zingeber officinale rhizome. Chemical detection of extract appeared that the ethanolic extract contain flavonoid, saponin, glycosids, phenols, resins, volatile oils, tannins, terpins and steroids while alkaloids and cumarines gave negative results. Different concentrations were prepared from ethanolic extract starting from (1-1000) µg/ml to evaluate the cytotoxic effect of ethanolic extract on two malignant cell lines, human laryngeal carcinoma (HEP-2) cell line and murine mammary adenocarcinoma (AMN-3) cell line, exposure periods of cell lines were measured at (24, 48, 72)hr in a microtitration plate under complete sterile conditions. Results showed that, Ethanolic crude extract exhibited time-dependent cytotoxic effect of all concentrations after exposure for 24hr on cancer and transformed cells lines, and the 100 µg/ml gave the highest inhibition rate for AMN-3 86.2% after 24hr of exposure time and the cytotoxic effect of the extract started at lowest concentrations on HEP-2 cell line after 48hr of exposure reached which the best concentration 600 µg/ml gave the highest inhibition cell growth 73.4% after 48hr of exposure time.

Effect of Ginger Extract on 4T1 Breast Cancer cell line in Balb/c Mouse.

2021 ◽  
Vol 08 ◽  
Author(s):  
Alireza Pooyae Golizadeh ◽  
Asa Ebrahimi ◽  
Mahdi Rahaie ◽  
Farzaneh Samiee
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Day Treatment ◽  
Cancer Cell Line ◽  
Ginger Extract ◽  
Suppressor Genes ◽  
4T1 Breast Cancer

Background: By considering [6]-gingerol as an important polyphenol, ginger has been identified to be related to cancer. Objective: In this study, the evaluation of ginger extract on the expressions of four genes from different categories involved in breast cancer process in Balb/C mouse after 14 days was the main objective. Methods: In term of this research, ethanol extraction methods for extracting ginger rhizomes with the goal of increasing [6]-gingerol were used. In this regard, MTT test was accomplished for whole ginger extract to confirm its effect on 4T1 breast cancer cell line (murine mammary carcinoma cell). Afterward, the transplanted breast cancer Balb/C mouse was used to be treated by whole ginger extract (0.02 g/kg of mouse weight). The expressions of STAT3, β-catenin, P53, APC, genes were evaluated, after 14-day treatment. Results: The results showed that 70% ethanol and microwave extraction method can be considered as the best method for extracting ginger extract. The results of the expression of β-catenin has decreased in blood and liver of the treated group. Moreover, the expression of STAT3 oncogenes has significantly decreased in the liver tissue. In addition, the expressions of APC suppressor genes have increased in Blood and liver. Conclusion: In conclusion, treatment by 0.02 g/kg of mouse weight of ginger extract after 14 days increased the expressions of APC as suppressor genes and β-catenin oncogene in Blood and liver of the breast cancer group.

Ultrastructure of NPC/HK1 cells heterotransplanted in athymic nude mice

1982 ◽  
Vol 40 ◽  
pp. 236-237
Author(s):  
E.C. Chew ◽  
C.L. Li ◽  
D.P. Huang ◽  
H.C. Ho ◽  
L.S. Mak ◽  
...  
Keyword(s):  
Cell Line ◽  
Nude Mice ◽  
Biopsy Specimen ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Recurrent Tumour ◽  
Athymic Nude Mice ◽  
Cell Line Establishment ◽  
Fine Structures ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been established from a biopsy specimen of a recurrent tumour of the nasopharynx which was histologically diagnosed as a moderately to well differentiated squamous cell carcinoma. A definite decrease in the amount of tonofilaments and desmosomes in the NPC/HK1 cells during the cell line establishment was observed. The present communication reports on the fine structures of the NPC/HK1 cells heterotraneplanted in athymic nude mice.

Ultrastructure of Choriocarcinoma in Vitro

1969 ◽  
Vol 27 ◽  
pp. 362-363
Author(s):  
John C. Garancis ◽  
R. A. Pattillo
Keyword(s):  
Cell Line ◽  
Physiological Function ◽  
Cell System ◽  
Bewo Cell ◽  
Bewo Cells ◽  
Gestational Choriocarcinoma ◽  
Bewo Cell Line

Growth of cell system (BeWo-cell line) derived from human gestational choriocarcinoma has been established and continuously maintained in-vitro. Furthermore, it is evident from the previous studies that this cell line has retained the physiological function of the placental trophoblasts, namely the synthesis of human chorionic gonadotrophil(HCG).The BeWo cells were relatively small and possessed single nuclei, thus indicating that this cell line consists exclusively of cytotrophoblasts. In some instances cells appeared widely separated and their lateral surfaces were provided with numerous microvilli (Fig.1).

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