scholarly journals Diversity of Phytophthora Communities across Different Types of Mediterranean Vegetation in a Nature Reserve Area

Forests ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 853
Author(s):  
Mario Riolo ◽  
Francesco Aloi ◽  
Federico La Spada ◽  
Saverio Sciandrello ◽  
Salvatore Moricca ◽  
...  
Keyword(s):  
Vegetation Type ◽  
Morphological Characteristics ◽  
Natura 2000 ◽  
Physicochemical Characteristics ◽  
Phytophthora Species ◽  
Nerium Oleander ◽  
Specific Objective ◽  
Different Types ◽  
Terrestrial Habitats ◽  
Sarcopoterium Spinosum

Research Highlights: Protected natural areas are a reservoir of Phytophthora species and represent the most suitable sites to study their ecology, being less disturbed by human activities than other environments. Background and Objectives: The specific objective of this study was to correlate the diversity and distribution of Phytophthora species with the vegetation in aquatic, riparian and terrestrial habitats within a protected area in Eastern Sicily, Southern Italy. Materials and Methods: Environmental samples (water and soil) were sourced from two streams running through the reserve and six different types of vegetation, including Platano-Salicetum pedicellatae, the Sarcopoterium spinosum community, Myrto communis-Pistacietum lentisci, Pistacio-Quercetum ilicis,Oleo-Quercetum virgilianae and a gallery forest dominated by Nerium oleander (Natura 2000 classification of habitats). Phytophthora species were recovered from samples using leaf baiting and were classified on the basis of morphological characteristics and sequencing of internal transcribed spacer (ITS) regions of ribosomal DNA (rDNA). Results: As many as 11 Phytophthora species, within five different ITS clades, were identified, including P. asparagi, P. bilorbang, P. cryptogea, P. gonapodyides, P. lacustris, P. multivora, P. nicotianae, P. oleae, P. parvispora, P. plurivora and P. syringae. No Phytophthora species were found in the Sarcopoterium spinosum comm. Phytophthora asparagi, P. lacustris and P. plurivora were the prevalent species in the other five plant communities, but only P. plurivora was present in all of them. Overall aquatic species from clade 6 (100 out of 228 isolates) were the most common; they were recovered from all five types of vegetation, streams and riparian habitats. Phytophthora populations found in the Platano-Salicetum pedicellatae and Oleo-Quercetum virgilianae show the highest diversity, while no correlation was found with the physicochemical characteristics of the soil. Conclusions: The vegetation type and the aquatic or terrestrial habitat were identified as major environmental factors correlated with the diversity of Phytophthora communities in this reserve.

Innovatory role of nanomaterials as bio-tools for treatment of cancer

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Khuram Shahzad Ahmad ◽  
Muntaha Talat ◽  
Shaan Bibi Jaffri ◽  
Neelofer Shaheen
Keyword(s):  
Cancer Treatment ◽  
Cancer Therapeutics ◽  
Physicochemical Characteristics ◽  
Global Scale ◽  
Current Review ◽  
Anti Cancer ◽  
Different Types ◽  
Cancerous Cells

AbstractConventional treatment modes like chemotherapy, thermal and radiations aimed at cancerous cells eradication are marked by destruction pointing the employment of nanomaterials as sustainable and auspicious materials for saving human lives. Cancer has been deemed as the second leading cause of death on a global scale. Nanomaterials employment in cancer treatment is based on the utilization of their inherent physicochemical characteristics in addition to their modification for using as nano-carriers and nano-vehicles eluted with anti-cancer drugs. Current work has reviewed the significant role of different types of nanomaterials in cancer therapeutics and diagnostics in a systematic way. Compilation of review has been done by analyzing voluminous investigations employing ERIC, MEDLINE, NHS Evidence and Web of Science databases. Search engines used were Google scholar, Jstore and PubMed. Current review is suggestive of the remarkable performance of nanomaterials making them candidates for cancer treatment for substitution of destructive treatment modes through investigation of their physicochemical characteristics, utilization outputs and long term impacts in patients.

scholarly journals Phytophthora Diversity in Pennsylvania Nurseries and Greenhouses Inferred from Clinical Samples Collected over Four Decades

2020 ◽  
Vol 8 (7) ◽  
pp. 1056
Author(s):  
Cody Molnar ◽  
Ekaterina Nikolaeva ◽  
Seonghwan Kim ◽  
Tracey Olson ◽  
Devin Bily ◽  
...  
Keyword(s):  
Plant Species ◽  
Morphological Characteristics ◽  
Phytophthora Species ◽  
Clinical Samples ◽  
Department Of Agriculture ◽  
Distinct Subgroup ◽  
Exotic Pathogens ◽  
Diverse Plant Species ◽  
Insight Into ◽  
Diverse Plant

The increasing movement of exotic pathogens calls for systematic surveillance so that newly introduced pathogens can be recognized and dealt with early. A resource crucial for recognizing such pathogens is knowledge about the spatial and temporal diversity of endemic pathogens. Here, we report an effort to build this resource for Pennsylvania (PA) by characterizing the identity and distribution of Phytophthora species isolated from diverse plant species in PA nurseries and greenhouses. We identified 1137 Phytophthora isolates cultured from clinical samples of >150 plant species submitted to the PA Department of Agriculture for diagnosis from 1975 to 2019 using sequences of one or more loci and morphological characteristics. The three most commonly received plants were Abies, Rhododendron, and Pseudotsuga. Thirty-six Phytophthora species identified represent all clades, except 3 and 10, and included a distinct subgroup of a known species and a prospective new species. Prominent pathogenic species such as P. cactorum, P. cinnamomi, P. nicotianae, P. drechsleri, P. pini, P. plurivora, and P. sp. kelmania have been found consistently since 1975. One isolate cultured from Juniperus horizontalis roots did not correspond to any known species, and several other isolates also show considerable genetic variation from any authentic species or isolate. Some species were isolated from never-before-documented plants, suggesting that their host range is larger than previously thought. This survey only provides a coarse picture of historical patterns of Phytophthora encounters in PA nurseries and greenhouses because the isolation of Phytophthora was not designed for a systematic survey. However, its extensive temporal and plant coverage offers a unique insight into the association of Phytophthora with diverse plants in nurseries and greenhouses.

scholarly journals Recent Advances in Enzymes for the Bioremediation of Pollutants

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Seyyed Mojtaba Mousavi ◽  
Seyyed Alireza Hashemi ◽  
Seyed Mohammad Iman Moezzi ◽  
Navid Ravan ◽  
Ahmad Gholami ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Azo Dyes ◽  
Membrane Filtration ◽  
Cost Effective ◽  
Physicochemical Characteristics ◽  
Pollutant Removal ◽  
Inorganic Pollutants ◽  
Environment Friendly ◽  
Different Types ◽  
Biological Methods

Nowadays, pollution of the environment is a huge problem for humans and other organisms’ health. Conventional methods of pollutant removal like membrane filtration or ion exchange are not efficient enough to lower the number of pollutants to standard levels. Biological methods, because of their higher efficiency and biocompatibility, are preferred for the remediation of pollutants. These cost-effective and environment-friendly methods of reducing pollutants are called bioremediation. In bioremediation methods, enzymes play the most crucial role. Enzymes can remedy different types of organic and inorganic pollutants, including PAHs, azo dyes, polymers, organocyanides, lead, chromium, and mercury. Different enzymes isolated from various species have been used for the bioremediation of pollutants. Discovering new enzymes and new subtypes with specific physicochemical characteristics would be a promising way to find more efficient and cost-effective tools for the remediation of pollutants.

scholarly journals On the diversity of the SE Indo-Pacific species ofTerebellides(Annelida; Trichobranchidae), with the description of a new species

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2313 ◽  
Author(s):  
Julio Parapar ◽  
Juan Moreira ◽  
Daniel Martin
Keyword(s):  
New Species ◽  
Morphological Characteristics ◽  
Relative Length ◽  
Andaman Sea ◽  
Species Discrimination ◽  
Pacific Waters ◽  
Routine Monitoring ◽  
Different Types ◽  
Pacific Species ◽  
A New Species

The study of material collected during routine monitoring surveys dealing with oil extraction and aquaculture in waters off Myanmar (North Andaman Sea) and in the Gulf of Thailand, respectively, allowed us to analyse the taxonomy and diversity of the polychaete genusTerebellides(Annelida). Three species were found, namelyTerebellidescf.woolawa,Terebellides hutchingsaespec. nov.(a new species fully described and illustrated), andTerebellidessp. (likely a new species, but with only one available specimen). The new species is characterised by the combination of some branchial (number, fusion and relative length of lobes and papillation of lamellae), and thoracic (lateral lobes and relative length of notopodia) characters and is compared with all species described or reported in the SW Indo-Pacific area, as well as with those sharing similar morphological characteristics all around the world. The taxonomic relevance of the relative length of branchial lobes and different types of ciliature in branchial lamellae for species discrimination in the genus is discussed. A key to allTerebellidesspecies described in SE Indo-Pacific waters is presented.

scholarly journals First Report of Phytophthora ramorum on Viburnum bodnantense in Belgium

Plant Disease ◽  
2003 ◽  
Vol 87 (2) ◽  
pp. 203-203 ◽  
Author(s):  
D. De Merlier ◽  
A. Chandelier ◽  
M. Cavelier
Keyword(s):  
The Netherlands ◽  
Plant Protection ◽  
Morphological Characteristics ◽  
Pcr Primers ◽  
Selective Medium ◽  
Phytophthora Species ◽  
Phytophthora Ramorum ◽  
Pathogenicity Test ◽  
First Report ◽  
Root Necrosis

In the past decade, a new Phytophthora species inducing shoot canker on Rhododendron and dieback of Viburnum has been observed in Europe, mainly in Germany and the Netherlands, and California. This new pathogen has been named Phytophthora ramorum (3). In May 2002, a diseased Viburnum plant (Viburnum bodnantense) from the Plant Protection Service (Ministry of Agriculture, Belgium) was submitted to our laboratory for diagnosis. Symptoms included wilting, leaves turning from green to brown, discolored vascular tissues, and root necrosis. The plant came from a Belgian ornamental nursery that obtained supplies of stock plants from the Netherlands. Pieces of necrotic root tissue were excised, surface-disinfected, and transferred aseptically to a Phytophthora selective medium. P. ramorum was identified based on morphological characteristics, including the production of numerous, thin-walled chlamydospores (25 to 70 µm in diameter, average 43 µm) and deciduous, semi-papillate sporangia arranged in clusters. Radial growth after 6 days at 20°C on V8 juice agar was 2.8 mm per day. Random amplified microsatellite markers (RAMS) (2) from the total genomic DNA of the Belgian strain (CBS 110901) were similar to those of P. ramorum reference strains (CBS 101330, CBS 101332, and CBS 101554). Using PCR primers specific for P. ramorum, the identification was confirmed by W. A. Man in't Veld (Plantenziektenkundige Dienst, Wageningen, the Netherlands) (1). A pathogenicity test was carried out on three sterile cuttings of Rhododendron catawbiense (3). Brown lesions were observed on the inoculated cuttings after 6 to 7 days. None of the three uninoculated cuttings showed symptoms of infection. P. ramorum was reisolated from lesion margins on the inoculated cuttings. To our knowledge, this is the first report of the fungus from Belgium. Since our initial observation, we have found P. ramorum in other Belgian nurseries on R. yakusimanum. References: (1) M. Garbelotto et al. US For. Ser. Gen. Tech. Rep. PSW-GRT. 184:765, 2002. (2) J. Hantula et al. Mycol. Res. 101:565, 1997. (3) S. Werres et al. Mycol. Res. 105:1155, 2001.

scholarly journals First Report of Phytophthora hydropathica Causing Wilting and Shoot Dieback on Viburnum in Italy

Plant Disease ◽  
2014 ◽  
Vol 98 (11) ◽  
pp. 1582-1582 ◽  
Author(s):  
S. Vitale ◽  
L. Luongo ◽  
M. Galli ◽  
A. Belisario
Keyword(s):  
Natural Infection ◽  
Morphological Characteristics ◽  
Leaf Tissue ◽  
The United States ◽  
Selective Medium ◽  
Phytophthora Species ◽  
Phytophthora Ramorum ◽  
Width Ratio ◽  
Morphological Identification ◽  
First Report

The genus Viburnum comprises over 150 species of shrubs and small trees such as Laurustinus (Viburnum tinus L.), which is one of the most widely used ornamental plants in private and public gardens. Furthermore, it commonly forms stands of natural woodland in the Mediterranean area. In autumn 2012, a survey was conducted to determine the presence of Phytophthora ramorum on Viburnum in commercial nurseries in the Latium region where wilting, dieback, and death of twigs were observed on 30% of the Laurustinus plants. A Phytophthora species was consistently recovered from soil rich in feeder roots from potted Laurustinus plants showing symptoms. Soil samples were baited with rhododendron leaves. Small pieces of leaf tissue cut from the margin of lesions were plated on P5ARPH selective medium (4). Pure cultures, obtained by single-hypha transfers on potato dextrose agar (PDA), were petaloid. Sporangia formation was induced on pepper seeds (3). Sporangia were almost spherical, ovoid or obpyriform, non-papillate and non-caducous, measuring 36.6 to 71.4 × 33.4 to 48.3 μm (average 53.3 × 37.4 μm) with a length/width ratio of 1.4. Chlamydospores were terminal and 25.2 to 37.9 μm in diameter. Isolates were considered heterothallic because they did not produce gametangia in culture or on the host. All isolates examined had 30 to 35°C as optimum temperatures. Based on these morphological characteristics, the isolates were identified as Phytophthora hydropathica (2). Morphological identification was confirmed by internal transcribed spacer (ITS), and mitochondrial partial cytochrome oxidase subunit 2 (CoxII) with BLAST analysis in the NCBI database revealing 99% identity with ITS and 100% identity with CoxII. The sequences of the three isolates AB234, AB235, and AB236 were deposited in European Nucleotide Archive (ENA) with the accession nos. HG934148, HG934149, and HG934150 for ITS and HG934151, HG934152, and HG934153 for CoxII, respectively. Pathogenicity tests were conducted in the greenhouse on a total of six 1-year-old shoots cut from V. tinus plants with two inoculation points each. Mycelial plugs cut from the margins of actively growing 8-day-old cultures on PDA were inserted through the epidermis into the phloem. Controls were treated as described above except that sterile PDA plugs replaced the inoculum. Shoots were incubated in test tubes with sterile water in the dark at 24 ± 2°C. After 2 weeks, lesions were evident at the inoculation points and symptoms were similar to those caused by natural infection. P. hydropathica was consistently re-isolated from the margin of lesions, while controls remained symptomless. In the United States in 2008, P. hydropathica was described as spreading from irrigation water to Rhododendron catawbiense and Kalmia latifolia (2). This pathogen can also attack several other horticultural crops (1), but to our knowledge, this is the first report of P. hydropathica causing wilting and shoot dieback on V. tinus. References: (1) C. X. Hong et al. Plant Dis. 92:1201, 2008. (2) C. X. Hong et al. Plant Pathol. 59:913, 2010. (3) E. Ilieva et al. Eur. J. Plant Path. 101:623, 1995. (4) S. N. Jeffers and S. B. Martin. Plant Dis. 70:1038, 1986.

Contribution of Composition, Physicochemical Characteristics and Polyphosphates to the Microbial Safety of Pasteurized Cheese Spreads

1994 ◽  
Vol 57 (4) ◽  
pp. 295-300 ◽  
Author(s):  
KARL F. ECKNER ◽  
WENDY A. DUSTMAN ◽  
ANNA A. RYŚ-RODRIGUEZ
Keyword(s):  
Clostridium Botulinum ◽  
Toxin Production ◽  
Physicochemical Characteristics ◽  
The Other ◽  
Microbial Safety ◽  
Processed Cheese ◽  
Moisture Contents ◽  
Glass Containers ◽  
Different Types ◽  
Process Cheese

Pasteurized process cheese spread was manufactured with moisture contents of 52, 54, 56 and 60%. Three different types of phosphate emulsifier were used, disodium ortho-phosphate and two commercially-available polyphosphates, S9 and S9H. Pasteurized, processed cheese spreads were inoculated with approximately 1 × 104 Clostridium botulinum spores/gram cheese in the cook kettle, held 3 min at 80°C, hot-filled into glass containers, and incubated at 30°C. Samples were analyzed over 30 weeks for growth of C. botulinum and toxigenesis. Toxin was first detected in 60% moisture cheese with disodium ortho-phosphate as the emulsifier at 8 weeks and in 60% moisture cheese with the test polyphosphates as the emulsifier when tested at 20 weeks. None of the other cheese formulations were toxic at 20 weeks. Toxin production correlated statistically to time, moisture, pH and phosphate type.

scholarly journals Morphological Characteristics and Clinical Significance of Different Types of Tumor Vessels in Patients with Stages I-IIA of Squamous Cervical Cancer

2020 ◽  
Vol 2020 ◽  
pp. 1-20 ◽  
Author(s):  
Marina A. Senchukova ◽  
Elena V. Makarova ◽  
Elena I. Shurygina ◽  
Nadezhda N. Volchenko
Keyword(s):  
Cervical Cancer ◽  
Clinical Significance ◽  
Lymphatic Vessels ◽  
Morphological Characteristics ◽  
Solid Component ◽  
Contact Type ◽  
Tumor Microvessels ◽  
Squamous Cervical Cancer ◽  
Different Types ◽  
Early Cervical Cancer

The determination of factors associated with progression of cervical cancer is important, both for a recurrence risk assessment and for determining optimal treatment tactics. Previously, we showed the prognostic value of different types of tumor microvessels (MVs) in gastric and breast cancer. The object of this research was to study the morphology and clinical significance of different tumor microvessels in early cervical cancer. A total of 65 archived paraffin blocks of patients with I-IIA stages of squamous cervical cancer were investigated. Samples were stained with Mayer hematoxylin and immunohistochemically using antibodies to CD34, podoplanin, HIF-1a, and Snail. The eight types of tumor MVs differed in morphology were identified. It was established that only the dilated capillaries (DСs) with weak expression of CD34, the contact type DCs, the capillaries in tumor solid component, and the lymphatic vessels in the lymphoid and polymorphic cell infiltrates of tumor stroma are associated with clinical and pathological characteristics of early cervical cancer. Preliminary results also suggest that a combination of fragmentation in tumor solid component and the contact type DCs may predict a recurrence of early cervical cancer. Given the small number of cervical cancer recurrences, the predictive significance of the described markers requires a more thorough examination.

scholarly journals A Quick Method for the Texture Mapping of Meshes Acquired by Laser Scanner

2012 ◽  
Vol 9 ◽  
pp. 17-26 ◽  
Author(s):  
Francesco Gabellone ◽  
Ivan Ferrari ◽  
Francesco Giuri
Keyword(s):  
Laser Scanner ◽  
Morphological Characteristics ◽  
Texture Mapping ◽  
Operational Effectiveness ◽  
Quick Method ◽  
Simple Method ◽  
Infra Red ◽  
Different Types ◽  
Infra Red Images ◽  
False Colour

The methodology described in this article was developed in connection with two different projects and entails texture mapping by time-of-flight laser scanner. In order to verify its operational effectiveness and applicability to other contexts, sites with extremely different morphological characteristics were studied. The basic rationale of this simple method derives from the need to obtain different types of mapping – including RGB real colour images, infra-red images, false colour images from georadar scans, etc. – from the same scanned surface. To resolve this problem, we felt that the most appropriate step was to obtain a UVW mapping based on the high resolution real colour images and then use the samecoordinates to rapidly map the false colour images as well. Thus we fitted a device to the camera to determine its trajectory (similar to a gunsight); when scanned by the laser scanner in the same context as the monument, it makes it possible to know the exact coordinates of the viewpoint.

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