scholarly journals Potassium-Solubilizing Activity of Bacillus aryabhattai SK1-7 and Its Growth-Promoting Effect on Populus alba L.

Forests ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1348
Author(s):  
Yifan Chen ◽  
Jianren Ye ◽  
Qingqing Kong

In the present study, the potassium-solubilizing characteristics of Bacillus aryabhattai SK1-7 and its growth-promoting effect on plants were evaluated to determine the biotechnological potential of this bacterium in alleviating soil potassium deficiency. The potassium-solubilizing activity of SK1-7 was determined by fermentation. Additionally, the fermentation broth was determined by flame spectrophotometry. The aluminum and silicon ion contents in SK1-7 fermentation broth were determined by inductively coupled plasma-atomic emission spectroscopy (ICP-AES) after digestion with nitric acid hydrogen peroxide hydrofluoric acid. Scanning electron microscopy (SEM)-based observations were performed to assess the morphological changes in potassium feldspar surfaces digested by potassium-solubilizing bacteria. In addition, the effects of SK1-7 on plant growth and soil physical and chemical properties were analyzed. After incubation for 7 days in a potassium-solubilizing medium, the concentration of potassium dissolved reached 10.8 μg/mL and the percentage of potassium released was 32.6%. The pH rapidly decreased from 7.2 to 4.321 within the first day and then further decreased to 3.90 after 7 days. After 7 days, the concentrations of aluminum and silicon in the fermentation broth were 1.01 and 24.19 μg/mL, respectively. The growth promotion assay results showed that SK1-7 has good growth-promoting effects on poplar and can effectively improve the available potassium content in poplar rhizosphere soil. The SK1-7 strain can effectively dissolve insoluble potassium to release soluble potassium ions and clearly promotes the growth of poplar after being applied to soil. Thus, the SK1-7 strain is a potassium-solubilizing microorganism with good application prospects.

1973 ◽  
Vol 56 (2) ◽  
pp. 235-243 ◽  
Author(s):  
M. WALLIS ◽  
JENNIFER A. DEW

SUMMARY Pituitary growth hormone has a dose-dependent growth promoting effect in pituitary dwarf mice (Snell's strain), and this effect can be used as the basis of a bioassay for the hormone. Prolactin and thyroxine also promote growth in these animals, and the effects of these hormones in combination with growth hormone were studied, in order to see whether their presence might enhance the precision or sensitivity of the growth hormone assay. When prolactin and/or thyroxine were administered with growth hormone, the growth response observed was no greater than the sum of the effects of the hormones given separately; in some cases it was less. Neither prolactin nor thyroxine increase the sensitivity or precision of the growth hormone bioassay. The implications of these results for theories about the mechanisms of growth promotion by these hormones are considered.


Author(s):  
Zhao L ◽  
◽  
Zhao W ◽  
Deng H ◽  
◽  
...  

Some fungi may promote plant growth by production of siderophores, Indole Acetic Acid (IAA) and phosphorus dissolving capability. In this study, eight fungi were isolated from the mushroom substrate, and their siderophores production, IAA production and phosphorus dissolving traits were determined. Although there was no significant difference in IAA production among the eight fungi, but the strain M13026-2 was a fungus with strong growth promoting traits compared with other seven fungi. In order to study the correlation between the growth promoting effect of cucumber pot culture and the above three traits, five fungi with different strength of traits were tested in pot. As a result, M13026- 2 which was identified as Talaromyces purpureogenus could significantly improve the growth parameters of cucumber seedlings, and could colonize in the rhizosphere soil and the tissue of cucumber stably. All the results suggested that the most relevant to their ability to promote plant growth is the trait of phosphorus dissolving, followed by siderophores production. The results of this study will provide scientific basis for the efficient selection and identification of a large number of fungi resources with the function of promoting plant growth, and reveal the good application potential of T. purpureogenus in agriculture fields.


2021 ◽  
Vol 12 ◽  
Author(s):  
Dominique Comeau ◽  
Carole Balthazar ◽  
Amy Novinscak ◽  
Nadia Bouhamdani ◽  
David L. Joly ◽  
...  

Plant growth-promoting rhizobacteria (PGPR) deploy several mechanisms to improve plant health, growth and yield. The aim of this study was to evaluate the efficacy of two Pseudomonas spp. strains and three Bacillus spp. strains used as single treatments and in consortia to improve the yield of Cannabis sativa and characterize the impact of these treatments on the diversity, structure and functions of the rhizosphere microbiome. Herein, we demonstrate a significant C. sativa yield increase up to 70% when inoculated with three different Pseudomonas spp./Bacillus spp. consortia but not with single inoculation treatments. This growth-promoting effect was observed in two different commercial soil substrates commonly used to grow cannabis: Promix and Canna coco. Marker-based genomic analysis highlighted Bacillus spp. as the main modulator of the rhizosphere microbiome diversity and Pseudomonas spp. as being strongly associated with plant growth promotion. We describe an increase abundance of predicted PGPR metabolic pathways linked with growth-promoting interactions in C. sativa.


2019 ◽  
Vol 116 (31) ◽  
pp. 15735-15744 ◽  
Author(s):  
Anna Koprivova ◽  
Stefan Schuck ◽  
Richard P. Jacoby ◽  
Irene Klinkhammer ◽  
Bastian Welter ◽  
...  

Plants in their natural ecosystems interact with numerous microorganisms, but how they influence their microbiota is still elusive. We observed that sulfatase activity in soil, which can be used as a measure of rhizosphere microbial activity, is differently affected by Arabidopsis accessions. Following a genome-wide association analysis of the variation in sulfatase activity we identified a candidate gene encoding an uncharacterized cytochrome P450, CYP71A27. Loss of this gene resulted in 2 different and independent microbiota-specific phenotypes: A lower sulfatase activity in the rhizosphere and a loss of plant growth-promoting effect by Pseudomonas sp. CH267. On the other hand, tolerance to leaf pathogens was not affected, which agreed with prevalent expression of CYP71A27 in the root vasculature. The phenotypes of cyp71A27 mutant were similar to those of cyp71A12 and cyp71A13, known mutants in synthesis of camalexin, a sulfur-containing indolic defense compound. Indeed, the cyp71A27 mutant accumulated less camalexin in the roots upon elicitation with silver nitrate or flagellin. Importantly, addition of camalexin complemented both the sulfatase activity and the loss of plant growth promotion by Pseudomonas sp. CH267. Two alleles of CYP71A27 were identified among Arabidopsis accessions, differing by a substitution of Glu373 by Gln, which correlated with the ability to induce camalexin synthesis and to gain fresh weight in response to Pseudomonas sp. CH267. Thus, CYP71A27 is an additional component in the camalexin synthesis pathway, contributing specifically to the control of plant microbe interactions in the root.


2017 ◽  
Vol 57 (2) ◽  
pp. 144-151 ◽  
Author(s):  
Arun Karnwal

AbstractThe use of plant growth promoting rhizobacteria is increasing in agriculture and gives an appealing manner to replace chemical fertilizers, pesticides, and dietary supplements. The objective of our research was to access the plant growth promotion traits ofPseudomonas aeruginosa,P. fluorescensandBacillus subtilisisolated from the maize (Zea maysL.) rhizosphere.In vitrostudies showed that isolates have the potential to produce indole acetic acid (IAA), hydrogen cyanide, phosphate solubilisation, and siderophore. RNA analysis revealed that two isolates were 97% identical toP. aeruginosastrain DSM 50071 andP. aeruginosastrain NBRC 12689 (AK20 and AK31), while two others were 98% identical toP. fluorescensstrain ATCC 13525,P. fluorescensstrain IAM 12022 (AK18 and AK45) and one other was 99% identical toB. subtilisstrain NCDO 1769 (AK38). Our gnotobiotic study showed significant differences in plant growth variables under control and inoculated conditions. In the present research, it was observed that the isolated strains had good plant growth promoting effects on rice.


2019 ◽  
Author(s):  
G. Praveen Kumar ◽  
Suseelendra Desai ◽  
Bruno M. Moerschbacher ◽  
Nour Eddine-El Gueddari

AbstractInoculation of crop plants with PGPR has in a large number of investigations resulted in increased plant growth and yield both in the greenhouse and in the field. This plant growth promoting effect of bacteria could be due to net result of synergistic effect of various pgpr traits that they exert in the rhizosphere region of the plant. Four (04) bacterial strain of fluorescent Pseudomonas spp. viz. P1, P17, P22 and P28 were identified previously for their plant growth promoting nature and abiotic stress tolerance and selected further to assess their chitinolytic activity and growth promotion on sorghum in combination with chitosans of low and high degree of acetylation. It was found that P1 has no chitin degrading nature and rest of the three strains have this property. When studied for their ability to grow in presence of chitosans of DA 1.6, 11, 35 and 56% all the strains showed growth in presence of chitosans. Seed bacterization of sorghum seeds with 04 bacterial strains in the presence and absence of chitosans (both low and high DA) and assessment of plant growth promotion after 15 days of sowing showed that P17+DA 56% chitosan combination showed higher growth of seedlings in plant growth chamber with highest root length of 25.9 cm, highest shoot length of 32.1 cm and dry mass of 132.7 mg/ plant. In P17+DA 56% chitosan treated seedlings various defence enzymes and PR-proteins were found to be present in highest quantities as compared to P1 and un-inoculated controls. Since this strain showed highest growth promotion of sorghum seedlings chitin-chitosan modifying enzyme (CCME) of this strain was partially characterized using different proteomic tools and techniques. CCME of P17 had one active polypeptide with a Pi in the range of 3.0-4.0. The digestion pattern of acetylated and deacetylated chitosans showed that P17 enzyme has endochitinase activity. Substrate specificity assay showed that the enzyme had more specificity towards highly acetylated chitosans. Two dimensional PAGE and MS analysis of the protein revealed similarities of this enzyme with protein of Pseudomonas aeruginosa chitinase PA01 strain of GenBank. In conclusion, the study established the option of opening new possibilities for developing bacterial-chitosan (P17+DA 56% chitosan) product for plant growth promotion and induced systemic resistance in sorghum.


2021 ◽  
Author(s):  
Ding Changhong ◽  
Shouyu Wang ◽  
Jiabin Li ◽  
Zhenyue Wang

Abstract Rumex gmelinii Turcz. (RGT) is a medicinal plant of Rumex in Polygonaceae. Our research group isolated an endophytic fungus plectosaphaerella cucumerina (Strain J-G) from RGT, which could promote the growth of the host significantly when was co-cultured with the host. In this study, we mainly used transcriptomic analysis to explore the molecular mechanism of the growth promoting effect. It was found that during the process of co-culture with Strain J-G, metabolism of amino acids, the synthesis and metabolism of carbohydrates in RGT tissue culture seedlings were all promoted, which provided sufficient material and energy basis for the growth of plants. In addition, response to hormones such as auxin and cytokinin in RGT tissue culture seedlings was improved significantly, which promoted the growth and development of plants. The repairing ability of DNA in RGT tissue culture seedlings was also enhanced to provide a good guarantee for the growth of plants. Meanwhile, defense system of RGT tissue culture seedlings was also mobilized by the existence of Strain J-G, therefore more secondary metabolites and stress resistant substances were produced to ensure the normal growth and metabolism of plants.


2020 ◽  
Vol 10 (5) ◽  
pp. 368-375
Author(s):  
Manu Bharti ◽  
Alankar Shrivastav ◽  
M. Abid ◽  
Najam Ali Khan

This review presents an overview on plants identified to possess hair growth activity in various ethno-botanical studies and surveys of tradition medicinal plants. There are various causes for hair loss and the phenomenon is still not fully understood. The treatments offered include both natural or synthetic products to treat the condition of hair loss (alopecia), nonetheless natural products are continuously gaining popularity mainly due to their fewer side effects and better formulation strategies for natural product extracts. Plants have been widely used for hair growth promotion since ancient times as reported in Ayurveda, Chinese and Unani systems of medicine. This review covers information about different herbs and herbal formulation that are believed to be able to reduce the rate of hair loss and at the same time stimulate new hair growth. A focus is placed on their mechanism of action and the review also covers various isolated phytoconstituents possessing hair growth promoting effect. Keywords: Alopecia, ayurveda, hair, herbal formulation.


2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Xingjie Lan ◽  
Jing Zhang ◽  
Zhaofeng Zong ◽  
Qing Ma ◽  
Yang Wang

A fungus with broad spectrum antifungal activity was isolated from the soil in Qinling Mountain, Shaanxi Province, in China. The fungus was identified as Purpureocillium lilacinum based on ITS rDNA gene analysis. The strain, coded as QLP12, showed high inhibition activity on fungal mycelium growth in vitro, especially to Mucor piriformis, Trichothecium roseum, Rhizoctonia solani, and Verticillium dahliae, and its potential for biocontrol efficacy of eggplant. Verticillium wilt disease caused by Verticillium dahliae among 10 fungal species tested was explored. In greenhouse experiments, QLP12 showed an excellent growth-promoting effect on eggplant seed germination (76.7%), bud growth (79.4%), chlorophyll content (47.83%), root activity (182.02%), and so on. QLP12 can colonize the eggplant interior and also develop in rhizosphere soil. In greenhouse, the incidence of Verticillium wilt decreased by 83.82% with pretreated QLP12 fermentation broth in the soil. In the field, QLP12 showed prominent biocontrol effects on Verticillium wilt by reducing the disease index over the whole growth period, a decline of 40.1%. This study showed that the strain QLP12 is not only an effective biocontrol agent for controlling Verticillium wilt of eggplant, but also a plant growth-promoting fungus that deserves to be further developed.


1990 ◽  
Vol 80 (1) ◽  
pp. 109-113 ◽  
Author(s):  
Ester P. Lorences ◽  
Gordon J. McDougall ◽  
Stephen C. Fry

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