Antimicrobial Activity of Se-Nanoparticles from Bacterial Biotransformation

Selenium nanoparticles (SeNPs) are gaining importance in the food and medical fields due to their antibacterial properties. The microbial inhibition of these kinds of particles has been tested in a wide range of Gram (+) and Gram (−) pathogenic bacteria. When SeNPs are synthesized by biological methods, they are called biogenic SeNPs, which have a negative charge caused by their interaction between surface and capping layer (bioorganic material), producing their high stability. This review is focused on SeNPs synthesis by bacteria and summarizes the main factors that influence their main characteristics: shape, size and surface charge, considering the bacteria growth conditions for their synthesis. The different mechanisms of antimicrobial activity are revised, and this review describes several biosynthesis hypotheses that have been proposed due to the fact that the biological mechanism of SeNP synthesis is not fully known.

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Antioxidant and Antimicrobial Activities of the Fresh aerial parts of Ammi visnaga L. from Algeria

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.6(2).p70-76 ◽

2016 ◽

Vol 6 (2) ◽

pp. 70-76

Author(s):

Soumia Keddari ◽

Narimen Benaoum ◽

Yasmina Mokhtaria Boufadi ◽

Mansouria Belhocine ◽

Ali Riazi

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Phenolic Compounds ◽

Biological Activities ◽

Antibacterial Properties ◽

Ethanolic Extract ◽

Diffusion Method ◽

E Coli ◽

Ammi Visnaga ◽

Wide Range

Medicinal plants have been used for countries as cures for human diseases because they contain components of therapeutic value. Among these medi-cinal plants, Ammi visnage which have an immense reservoir of potential compounds attributed to the secondary metabolites which have the advan-tage of being of great diversity of chemical structure and have a very wide range of biological activities. The objectives of the present work were to stu-dy the antioxidant and antimicrobial activity of phenolic compounds ex-tracted from A. visnaga L. Its extraction is performed by two methods, etha-nol extraction and water extraction. The results showed that A. visnaga L.. ethanolic extract contains a mixture of phytochemical classes as polyphenol, flavonoids and revealed that this plant has high antioxidant activity (IC50 0.069 mg/ml). Regarding the antimicrobial activity results expressed by the diameter of the inhibition zones by diffusion method AWDT, the most signifi-cant inhibition was observed against to Staphylococcus aureus (12 mm) to the ethanol extract at concentration of 100mg / ml. Thus the aqueous ex-tract had a significant inhibitory activity against on the strains Staphylococ-cus aureus (8 mm), E. coli ATCC 10536 (8 mm) to a concentration of 100 mg / ml. The results for the antibacterial properties have shown that Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and M. luteus.) were more sensitive than gram-negative (Pseudomonas aeruginosa, E. coli ATCC 10536) against from the action of phenolic compounds of the Ammi visnaga ethanolic extract.

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Screening, Identification and Efficacy Evaluation of Antagonistic Bacteria for Biocontrol of Soft Rot Disease Caused by Dickeya zeae

Microorganisms ◽

10.3390/microorganisms8050697 ◽

2020 ◽

Vol 8 (5) ◽

pp. 697 ◽

Cited By ~ 1

Author(s):

Jieling Li ◽

Ming Hu ◽

Yang Xue ◽

Xia Chen ◽

Guangtao Lu ◽

...

Keyword(s):

Antimicrobial Activity ◽

Prevention And Control ◽

Pathogenic Bacteria ◽

Soft Rot ◽

Antagonistic Bacteria ◽

Bacterial Soft Rot ◽

Soft Rot Disease ◽

Wide Range ◽

Rot Disease ◽

And Control

Dickeya zeae is the causal agent of bacterial soft rot disease, with a wide range of hosts all over the world. At present, chemical agents, especially agricultural antibiotics, are commonly used in the prevention and control of bacterial soft rot, causing the emergence of resistant pathogens and therefore increasing the difficulty of disease prevention and control. This study aims to provide a safer and more effective biocontrol method for soft rot disease caused by D. zeae. The spot-on-lawn assay was used to screen antagonistic bacteria, and three strains including SC3, SC11 and 3-10 revealed strong antagonistic effects and were identified as Pseudomonas fluorescens, P. parafulva and Bacillus velezensis, respectively, using multi-locus sequence analysis (MLSA) based on the sequences of 16S rRNA and other housekeeping genes. In vitro antimicrobial activity showed that two Pseudomonas strains SC3 and SC11 were only antagonistic to some pathogenic bacteria, while strain 3-10 had broad-spectrum antimicrobial activity on both pathogenic bacteria and fungi. Evaluation of control efficacy in greenhouse trials showed that they all restrained the occurrence and development of soft rot disease caused by D. zeae MS2 or EC1. Among them, strain SC3 had the most impressive biocontrol efficacy on alleviating the soft rot symptoms on both monocotyledonous and dicotyledonous hosts, and strain 3-10 additionally reduced the occurrence of banana wilt disease caused by Fusarium oxysporum f. sp. cubensis. This is the first report of P. fluorescens, P. parafulva and B. velezensis as potential bio-reagents on controlling soft rot disease caused by D. zeae.

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Synthesis and Antimicrobial Activity of Silver-Doped Hydroxyapatite Nanoparticles

BioMed Research International ◽

10.1155/2013/916218 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 72

Author(s):

Carmen Steluta Ciobanu ◽

Simona Liliana Iconaru ◽

Mariana Carmen Chifiriuc ◽

Adrian Costescu ◽

Philippe Le Coustumer ◽

...

Keyword(s):

Antimicrobial Activity ◽

Pathogenic Bacteria ◽

Antimicrobial Agents ◽

Antibacterial Properties ◽

Major Health Problem ◽

Major Health ◽

Wall Structures ◽

Nanocrystalline Hydroxyapatite ◽

Qualitative Assay ◽

Antibacterial And Antifungal

The synthesis of nanosized particles of Ag-doped hydroxyapatite with antibacterial properties is of great interest for the development of new biomedical applications. The aim of this study was the evaluation ofCa10−xAgx(PO4)6(OH)2nanoparticles (Ag:HAp-NPs) for their antibacterial and antifungal activity. Resistance to antimicrobial agents by pathogenic bacteria has emerged in the recent years and became a major health problem. Here, we report a method for synthesizing Ag doped nanocrystalline hydroxyapatite. A silver-doped nanocrystalline hydroxyapatite was synthesized at 100°C in deionised water. Also, in this paper Ag:HAp-NPs are evaluated for their antimicrobial activity against Gram-positive and Gram-negative bacteria and fungal strains. The specific antimicrobial activity revealed by the qualitative assay is demonstrating that our compounds are interacting differently with the microbial targets, probably due to the differences in the microbial wall structures.

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Antibacterial Effects of Oak Fruit, Jaft, and Jaftex Herbal Mouthwash: A Review

10.21203/rs.2.11341/v1 ◽

2019 ◽

Author(s):

Maria Cheraghi ◽

Fatemeh Babadi

Keyword(s):

Antimicrobial Activity ◽

Pathogenic Bacteria ◽

Web Of Science ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Antibiotic Sensitivity ◽

Antibacterial Activities ◽

Antibacterial Effects ◽

Digital Literature ◽

Review Study

Abstract Recently, natural products have been evaluated as a source of antimicrobial agent with efficacies against a variety of microorganisms. The antibacterial activities of the oak fruit,inner husk of oak fruit (Jaft), and Jaftex mouthwash have been studiedin several studies. Thisstudy aimedto review the studies ofthe effects of antibacterial properties of the oak fruit, oak fruit hull (Jaft), and Jaftex mouthwash. Materials and Methods In this review study, relevant articles related to the antimicrobial activity of the oak fruit, inner husk of oak fruit (Jaft), and 'Jaftex mouthwash' were searched from the current digital literature using electronic databases namely, SID, ScienceDirect, PubMed, Google Scholar, Magiran, Web of Science employing the same keywords from 1990 to 2019. Results Oakfruit, inner husk of oak fruit (Jaft), and Jaftex mouthwash have antimicrobial properties against many microorganisms. Discussion and Conclusion Antibacterial properties of oak fruit, inner husk ofoak fruit (Jaft) can be used to reverse the antibiotic sensitivity against pathogenic bacteria. Jaftex is recommended as an antibacterial and anti-plaque mouthwash.

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IN VITRO ANTIMICROBIAL ACTIVITY OF SOME ESSENTIAL OILS AGAINST BACTERIAL PATHOGENS CAUSING SKIN DISEASES IN VAPOR PHASE

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2019.v11s5.t0084 ◽

2019 ◽

pp. 113-115

Author(s):

PURIT PATTANAPANIT ◽

SUNISA MITHONGLANG ◽

SUNITA MITHONGLANG ◽

SURACHAI TECHAOEI

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Antibacterial Properties ◽

Diffusion Method ◽

Syzygium Aromaticum ◽

Aromatic Plants ◽

Volatile Oils

Objective: The objective of this study was to evaluate the antimicrobial activity of volatile oils from aromatic plants against pathogenic bacteria.Methods: Thai aromatic plants such as Pogostemon cablin (Blanco) Benth (Patchouli oil), Cymbopogon nardus Rendle (Citronella grass oil), Pelargoniumroseum (Geranium oil), Syzygium aromaticum (L.) Merrill and Perry (clove oil), Cinnamomum spp.(cinnamon oil), and Cymbopogon citratus (DC.) Stapf.(lemongrass oil) were selected. Essential oils were obtained by water distillation and were stored at 4°C until use. Five human pathogenic bacteria wereobtained from Thai traditional Medicine College, Rajamangala University of Technology, Staphylococcus epidermidis, Escherichia coli, Staphylococcusaureus, methicillin-resistant S. aureus (MRSA), and Pseudomonas aeruginosa. The antibacterial activity of volatile oils was determined by disc-diffusionassay. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each essential oil were determined.Results: Our study showed that 10% of essential oil from Cinnamomum spp. was the most potential against S. aureus, MRSA, and E. coli when assayedby disc-diffusion method with inhibition zones ranging from 37.66±0.57 to 45.33±1.15 mm and from 29.33±0.57 to 36.00±1.00 for lemongrass oilwith MIC and MBC of 1.25%.Conclusion: From this study, it can be concluded that some essential oils have potential antibacterial activity. The present investigation providessupport to the antibacterial properties of essential oils and will be applied to health-care product as aroma antibacterial products.

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DETERMINATION OF ANTIBACTERIAL ACTIVITY OF SOME IMPORTANT SPICES

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v3.i10.2015.2932 ◽

2015 ◽

Vol 3 (10) ◽

pp. 57-64

Author(s):

Ranganathan Kapilan

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Maximum Activity ◽

Gram Negative Bacteria ◽

Alcoholic Extract ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Wide Range

Wide range of plant extracts are used for medicinal purposes as they are very cheap, efficient, harmless and do not cause any side effects. Spices are parts of different plants and they add special aroma and taste to the food preparations. The aim of the study was to determine the antimicrobial activity of some important naturally grown spices against gram positive and gram negative pathogenic bacteria. Antibacterial activity of the spices was tested against gram positive bacteria Bacillus pumilus, Bacillus cereus and Staphylococcus aureus and gram negative bacteria Escherichia coli, Salmonella typhi and Pseudomonas aeruginosa using aqueous, ethanolic, methanolic and liquid nutrient extracts. Among all the extracts tested alcoholic extracts of Cardamom (Elettaria cardamom), clove (Eugenia caryophyllus) and lemongrass (Cymbopogoncitratus) showed maximum antimicrobial activity against gram negative bacteria while alcoholic extract of Cardamom (Elettaria cardamom) and lemongrass (Cymbopogoncitratus) showed maximum activity against gram positive bacteria. All the spices tested in this study proved that they have antibacterial activity and the maximum activity index (1.39) was exhibited by the ethanol extract of cardamom against E.coli.

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Improving Phenolic-Linked Antioxidant, Antihyperglycemic and Antibacterial Properties of Emmer and Conventional Wheat Using Beneficial Lactic Acid Bacteria

Applied Microbiology ◽

10.3390/applmicrobiol1020020 ◽

2021 ◽

Vol 1 (2) ◽

pp. 270-288

Author(s):

Ashish Christopher ◽

Dipayan Sarkar ◽

Kalidas Shetty

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Pathogenic Bacteria ◽

Phenolic Content ◽

Bifidobacterium Longum ◽

Antibacterial Properties ◽

Vitro Assay

Beneficial lactic acid bacteria (LAB)-based fermentation is an effective bioprocessing approach to improve human-health-targeted functional benefits of plant-based food substrates, such as cereal grains. Previously, we observed high phenolic bioactive-linked antioxidant and anti-hyperglycemic properties in whole grain Emmer (hulled). In this study, beneficial LAB (Lactiplantibacillus plantarum) was recruited to ferment (0–72 h) aqueous extracts (0.4 g/mL concentration) of previously optimized hulled Emmer wheat and conventional red spring wheat cv. Barlow. The fermented and unfermented (control) wheat extracts were analyzed for phenolic content, phenolic profile, antioxidant activity, and antihyperglycemic properties (α-amylase and α-glucosidase enzyme inhibitory activity) using in vitro assay models. Additionally, antimicrobial activity against pathogenic bacteria Helicobacter pylori, and potential prebiotic activity supporting the growth of beneficial Bifidobacterium longum were also investigated. Improvement in antioxidant activity and antihyperglycemic functional benefits were observed, while soluble phenolic content remained high after 72 h fermentation. Antimicrobial activity against H. pylori was also observed in 48 and 72 h fermented wheat extracts. This study provides an insight into the efficacy of LAB-based fermentation as a safe bioprocessing tool to design health-targeted functional foods and ingredients from underutilized whole grains like Emmer for targeting type 2 diabetes dietary benefits.

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Production optimization, stability and oil emulsifying potential of biosurfactants from selected bacteria isolated from oil-contaminated sites

Royal Society Open Science ◽

10.1098/rsos.211003 ◽

2021 ◽

Vol 8 (10) ◽

Author(s):

Ferdausi Ali ◽

Sharup Das ◽

Tanim Jabid Hossain ◽

Sumaiya Islam Chowdhury ◽

Subrina Akter Zedny ◽

...

Keyword(s):

Pathogenic Bacteria ◽

Oil Pollution ◽

Environmental Safety ◽

Growth Conditions ◽

Production Optimization ◽

Contaminated Sites ◽

Rrna Gene ◽

Emulsification Index ◽

As Species ◽

Wide Range

Oil pollution is of increasing concern for environmental safety and the use of microbial surfactants in oil remediation has become inevitable for their efficacy and ecofriendly nature. In this work, biosurfactants of bacteria isolated from oil-contaminated soil have been characterized. Four potent biosurfactant-producing strains (SD4, SD11, SD12 and SD13) were selected from 27 isolates based on drop collapse assay and emulsification index, and identified as species belonging to Bacillus , Burkholderia , Providencia and Klebsiella , revealed from their 16S rRNA gene-based analysis. Detailed morphological and biochemical characteristics of each selected isolate were determined. Their growth conditions for maximum biosurfactant production were optimized and found quite similar among the four isolates with a pH of 3.0 and temperature 37°C after 6 or 7 days of growth on kerosene. The biosurfactants of SD4, SD11 and SD12 appeared to be glycolipids and that of SD13 a lipopeptide. Emulsification activity of most of the biosurfactants was stable at low and high temperatures (4–100°C), a wide range of pH (2–10) and salt concentrations (2–7% NaCl). Each biosurfactant showed antimicrobial activity against two or more pathogenic bacteria. The biosurfactants were well-capable of emulsifying kerosene, diesel and soya bean, and could efficiently degrade diesel.

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Antimicrobial activity of endophytic fungus Fusarium sp. isolated from medicinal honeysuckle plant

Archives of Biological Sciences ◽

10.2298/abs140401004z ◽

2016 ◽

Vol 68 (1) ◽

pp. 25-30 ◽

Cited By ~ 7

Author(s):

Huiru Zhang ◽

Xinchen Sun ◽

Chunping Xu

Keyword(s):

Antimicrobial Activity ◽

Endophytic Fungus ◽

Pathogenic Bacteria ◽

New Drugs ◽

Bioactive Metabolites ◽

Gel Chromatography ◽

Physiological Characterization ◽

Wide Range ◽

Fusarium Sp

Endophytes of plants have a wide range of strains comprising important sources of various bioactive metabolites. An endophytic fungus was isolated from honeysuckle, an important Chinese medicinal plant. The phylogenetic and physiological characterization indicated that the isolated strain JY2corresponded to Fusarium sp. The culture filtrate of JY2displayed antagonism activity against some pathogenic bacteria owing to the existence of antimicrobial compounds. The filtrate revealed the strongest in vitro antimicrobial activity on Pseudomonas aeruginosa by increasing the permeability of cell membranes. The antibacterial extract was fractionated and purifiedusing silica gel chromatography. Five different bioactive compounds were isolated by bioactivity-guided fractionation from the culture extracts of JY2, and preliminarily identified by HPLC-MS spectral data. These results suggest that Fusarium sp. features a potentially remarkable antimicrobial activity and could be valuable to discover the new drugs or agents for antimicrobial purposes.

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Isolation of pathogenic microorganisms from burn patient and in vitro determination of antibacterial activity of honey against antibiotic resistance isolates

International Journal of Scientific Reports ◽

10.18203/issn.2454-2156.intjscirep20203114 ◽

2020 ◽

Vol 6 (8) ◽

pp. 310

Author(s):

Ifra Tun Nur ◽

Tahmina Jahan ◽

Sharmin Akter

Keyword(s):

Antimicrobial Activity ◽

Pathogenic Bacteria ◽

Diffusion Method ◽

Pathogenic Microorganisms ◽

Zone Of Inhibition ◽

Burn Wound ◽

College Hospital ◽

Wide Range

Background: Honey is a natural therapeutic agent which manifest antimicrobial activity against a wide range of bacteria. Therefore, the current study was designed to isolate pathogenic bacteria from burn wound and also to determine the anti-bacterial traits of natural and processed honey against infectious agents.Methods: Wound samples were collected from burn unit of Dhaka Medical College Hospital and conventional cultural methods were applied to identify pathogenic microorganisms. A total of six samples including three each of natural and processed honey were tested for the determination of antimicrobial activity by agar well diffusion method. Results: Among ten wound samples highest load of total viable bacteria was recorded up to 3.7×106 cfu/ml. The maximum load of Pseudomonas spp. and Staphylococcus spp. were found up to 1.6×104 cfu/ml and 8.7×104 cfu/ml respectively. Significant in vitro antimicrobial activity was found in all the samples. Natural honey showed a little bit more efficacy than processed honey. The samples exhibited antibacterial traits against Staphylococcus aureus with a wide zone of inhibition and moderate zone of inhibition against Pseudomonas spp. when they are subjected to 100% concentered honey. E. coli and Klebsiella spp. were remained to be unaffected at 75% and 50% concentrated honey, while S. aureus and Pseudomonas spp. were found to be sensitive at those concentrations.Conclusions: The in vitro efficacy of different types of honey tested against the bacteria dependent on the type of honey and the concentration at which it was administered. In our study 100% concentred honey was more efficient in inhibiting all the tested isolates.

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