scholarly journals Non-Specific Binding and Cross-Reaction of ELISA: A Case Study of Porcine Hemoglobin Detection

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1708
Author(s):  
Xingyi Jiang ◽  
Meng Wu ◽  
Jonathan Albo ◽  
Qinchun Rao
Keyword(s):  
Specific Binding ◽  
Sandwich Elisa ◽  
Cross Reaction ◽  
Different Types ◽  
Control Food ◽  
Secondary Antibodies ◽  
Food Safety And Quality ◽  
Porcine Hemoglobin ◽  
Selection Of

Different types of enzyme-linked immunosorbent assays (ELISA) have been widely used to control food safety and quality. To develop an accurate and reproducible ELISA, false immunodetection results caused by non-specific binding (NSB) and cross-reaction must be prevented. During the case study of sandwich ELISA development for the detection of porcine hemoglobin (PHb), several critical factors leading to NSB and cross-reaction were found. First, to reduce the NSB of the target analyte, the selection of microplate and blocker was discussed. Second, cross-reactions between enzyme-labeled secondary antibodies and sample proteins were demonstrated. In addition, the function of (3-aminopropyl)triethoxysilane (APTES) was evaluated. Overall, this study highlights the essence of both antibody and assay validation to minimize any false-positive/negative immunodetection results.

scholarly journals FORECASTING OF SPORTS FIELDS CONSTRUCTION COSTS AIDED BY ENSEMBLES OF NEURAL NETWORKS

2019 ◽  
Vol 25 (7) ◽  
pp. 715-729 ◽  
Author(s):  
Michał Juszczyk ◽  
Krzysztof Zima ◽  
Wojciech Lelek
Keyword(s):  
Neural Networks ◽  
Cost Analysis ◽  
Construction Projects ◽  
Practical Implementation ◽  
Ensemble Averaging ◽  
Construction Costs ◽  
Different Types ◽  
Selection Of ◽  
Sports Fields

The paper presents an original approach to construction cost analysis and development of predictive models based on ensembles of artificial neural networks. The research was focused on the application of two alternative approaches of ensemble averaging that allow for combining a number of multilayer perceptron neural networks and developing effective models for cost predictions. The models have been developed for the purpose of forecasting construction costs of sports fields as a specific type of construction objects. The research included simulation and selection of numerous neural networks that became the members of the ensembles. The ensembles included either the networks of different types in terms of their structure and activation functions or the networks of the same type. The research also included practical implementation of the developed models for cost analysis based on a sports field BIM model. This case study examined and confirmed all of the four models’ predictive capabilities and superiority over models based on single networks for the particular problem. Verification including testing and the case study enabled selection of the best ensemble-based model that combined ten networks of different types. The proposed approach is prospective for fast cost analyses and conceptual estimates in construction projects.

scholarly journals Planning procedures towards high climatic quality cities. Example referring to Lisbon

Finisterra ◽  
2012 ◽  
Vol 41 (82) ◽  
Author(s):  
Maria João Alcoforado
Keyword(s):  
Urban Climate ◽  
Urban Morphology ◽  
Information Analysis ◽  
Different Types ◽  
Urban Heat ◽  
Planning Strategies ◽  
Physical Features ◽  
The City ◽  
Selection Of

The different stages of research on high climatic quality city are presented in this paper: acquisition of appropriate urban climate information, analysis of different urban climate features and selection of planning strategies (accordingly with the climate zone). Then, with the aid of a GIS, systematic guidelines for planning can be given. As a result, different types of benefits for city dwellers are to be expected (comfort, health, economic). Lisbon is presented as a case study. It is shown how the “translation” of the knowledge of Lisbon’s urban climate into simple guidelines for urban planning in order to mitigate the urban heat island, promote ventilation and increase air quality, was carried out. To this end, maps of the physical features of the city of Lisbon were drawn, synthesised into “urban homogeneous units” map. Climate guidelines are specified for each of the six groups of units (brought together according to urban morphology, topography and position in town.

Topik-Topik Komunikasi Dakwah Dalam Mesej Akidah Kepada Masyarakat Orang Asli di Selangor

Sains Insani ◽  
2018 ◽  
Vol 3 (2) ◽  
pp. 15-21
Author(s):  
Zulkefli Aini ◽  
Abdul Ghafar Don ◽  
Ahmad Irdha Mokhtar ◽  
Nur Uswah Ahmad Fauzi
Keyword(s):  
Qualitative Study ◽  
Study Design ◽  
Verbal Feedback ◽  
Structured Interviews ◽  
Orang Asli ◽  
Case Study Design ◽  
Beliefs And Practice ◽  
Islamic Faith ◽  
Selection Of

One of the factors that can affect a person's behavior is a communication message. In the context of the da`wah, preachers who involved actively in da`wah communication with the Orang Asli should be able to ensure that the message conveyed can be understood by the target group. In addition, the selection of the correct messages of da`wah based on the foremost priority simplify the process of sharing information between the preachers and the Orang Asli. Accordingly, this article aims to identify specific topics of Islamic faith (akidah) submitted by the Orang Asli in the process of da`wah communication and to identify verbal feedback given by the preachers to the Orang Asli of the topics. This qualitative study using case study design and data was collected through semi-structured interviews. Interviews involving nine preachers were active in da`wah activity to the Orang Asli community in Selangor. Data were analyzed thematically according to the specified objectives. The study found that the topics frequently raised by the Orang Asli is concerned about belief in Allah, belief in Malaikat, and belief in Qada' and Qadar. The topics may be found within the framework of worldview in their beliefs and practice of ancient traditions. Therefore, the preachers gave verbal feedback on these topics is based on a clear and precise sample corresponding to the level of their thinking. The emphasis on these topics is very important to strengthen and purify the faith of the community.Keywords: Communication; Message; Preacher; Indigenous community Abstrak: Elemen mesej dalam komunikasi merupakan salah satu faktor yang dapat memberi kesan terhadap perubahan tingkah laku seseorang. Dalam konteks dakwah, pendakwah yang terlibat dalam proses komunikasi dakwah dengan Orang Asli seharusnya berkebolehan memastikan kandungan mesej yang disampaikan boleh difahami oleh sasaran dakwahnya. Di samping itu, pemilihan mesej dakwah yang betul mengikut keutamaan memudahkan proses perkongsian maklumat antara pendakwah dengan Orang Asli. Sehubungan dengan itu, artikel ini bertujuan untuk mengenalpasti topik-topik tertentu dalam mesej akidah yang dikemukakan oleh masyarakat Orang Asli kepada pendakwah dan mengenalpasti maklum balas lisan yang diberikan oleh pendakwah kepada Orang Asli terhadap topik tersebut. Kajian kualitatif ini menggunakan reka bentuk kajian kes dengan pengumpulan data melalui temu bual separa struktur. Temu bual melibatkan sembilan orang pendakwah yang aktif dalam aktiviti dakwah masyarakat Orang Asli di Selangor. Data kajian dianalisis secara tematik mengikut objektif yang ditentukan. Kajian ini mendapati bahawa topik-topik yang sering dikemukan oleh Orang Asli kepada pendakwah dalam penyampaian mesej akidah ialah berkenaan tentang keimanan kepada Allah SWT, keimanan kepada malaikat, dan keimanan kepada qada’ dan qadar. Topik-topik berkenaan didapati berada dalam kerangka worldview kepercayaan dan amalan tradisi mereka. Sehubungan dengan itu, pendakwah memberikan maklum balas lisan terhadap topik-topik tersebut adalah berdasarkan keterangan yang jelas dan contoh yang tepat bersesuaian dengan tahap pemikiran mereka. Penekanan terhadap topik-topik tersebut merupakan perkara yang penting dalam rangka mengukuhkan dan memurnikan akidah masyarakat Orang Asli.Kata kunci: Komunikasi; Mesej; Pendakwah; Komuniti Orang Asli

Participatory Mapping in Browns Canyon National Monument, Colorado (USA)

2017 ◽  
Vol 1 (1) ◽  
pp. 1-16
Author(s):  
John Harner ◽  
Lee Cerveny ◽  
Rebecca Gronewold
Keyword(s):  
Land Management ◽  
Planning Process ◽  
Management Plan ◽  
Bureau Of Land Management ◽  
National Monument ◽  
Resource Managers ◽  
Different Types ◽  
Us Forest Service ◽  
Natural Resource Managers

Natural resource managers need up-to-date information about how people interact with public lands and the meanings these places hold for use in planning and decision-making. This case study explains the use of public participatory Geographic Information System (GIS) to generate and analyze spatial patterns of the uses and values people hold for the Browns Canyon National Monument in Colorado. Participants drew on maps and answered questions at both live community meetings and online sessions to develop a series of maps showing detailed responses to different types of resource uses and landscape values. Results can be disaggregated by interaction types, different meaningful values, respondent characteristics, seasonality, or frequency of visit. The study was a test for the Bureau of Land Management and US Forest Service, who jointly manage the monument as they prepare their land management plan. If the information generated is as helpful throughout the entire planning process as initial responses seem, this protocol could become a component of the Bureau’s planning tool kit.

Effective Altruism

2019 ◽  
Keyword(s):  
Animal Welfare ◽  
Poverty Alleviation ◽  
Future Generations ◽  
Global Poverty ◽  
Global Community ◽  
Helping Others ◽  
The World ◽  
Different Types ◽  
Effective Altruism

The effective altruism movement consists of a growing global community of people who organize significant parts of their lives around two key ideas, represented in its name. Altruism: If we use a significant portion of the resources in our possession—whether money, time, or talents—with a view to helping others, we can improve the world considerably. Effectiveness: When we do put such resources to altruistic use, it is crucial to focus on how much good this or that intervention is reasonably expected to do per unit of resource expended (for example, per dollar donated). While global poverty is a widely used case study in introducing and motivating effective altruism, if the ultimate aim is to do the most good one can with the resources expended, it is far from obvious that global poverty alleviation is highest priority cause area. In addition to ranking possible poverty-alleviation interventions against one another, we can also try to rank interventions aimed at very different types of outcome against one another. This includes, for example, interventions focusing on animal welfare or future generations. The scale and organization of the effective altruism movement encourage careful dialogue on questions that have perhaps long been there, throwing them into new and sharper relief, and giving rise to previously unnoticed questions. In the present volume, the first of its kind, a group of internationally recognized philosophers, economists, and political theorists contribute in-depth explorations of issues that arise once one takes seriously the twin ideas of altruistic commitment and effectiveness.

Transdiagnostic Approaches With Children

2018 ◽  
Author(s):  
Andrea B. Temkin ◽  
Mina Yadegar ◽  
Christine Cho ◽  
Brian C. Chu
Keyword(s):  
Mental Disorders ◽  
Externalizing Symptoms ◽  
Case Conceptualization ◽  
Common Elements ◽  
Transdiagnostic Treatment ◽  
Treatment Models ◽  
Different Types ◽  
Internalizing And Externalizing ◽  
Internalizing And Externalizing Symptoms

In recent years, the field of clinical psychology has seen a growing movement toward the research and development of transdiagnostic treatments. Transdiagnostic approaches have the potential to address numerous issues related to the development and treatment of mental disorders. Among these are the high rates of comorbidity across disorders, the increasing need for efficient protocols, and the call for treatments that can be more easily disseminated. This chapter provides a review of the current transdiagnostic treatment approaches for the treatment of youth mental disorders. Three different types of transdiagnostic protocols are examined: mechanism-based protocols, common elements treatments, and general treatment models that originated from single-disorder approaches to have broader reach. A case study illuminates how a mechanism-based approach would inform case conceptualization for a client presenting with internalizing and externalizing symptoms and how a transdiagnostic framework translates into practice.

scholarly journals Multi-Criteria Selection of Additives in Porous Asphalt Mixtures Using Mechanical, Hydraulic, Economic, and Environmental Indicators

2021 ◽  
Vol 13 (4) ◽  
pp. 2146
Author(s):  
Anik Gupta ◽  
Carlos J. Slebi-Acevedo ◽  
Esther Lizasoain-Arteaga ◽  
Jorge Rodriguez-Hernandez ◽  
Daniel Castro-Fresno
Keyword(s):  
Cellulose Fiber ◽  
Environmental Indicators ◽  
Asphalt Mixtures ◽  
Environmental Benefits ◽  
Modified Bitumen ◽  
Porous Asphalt ◽  
Different Types ◽  
Product Assessment ◽  
Polymer Modified Bitumen ◽  
Selection Of

Porous asphalt (PA) mixtures are more environmentally friendly but have lower durability than dense-graded mixtures. Additives can be incorporated into PA mixtures to enhance their mechanical strength; however, they may compromise the hydraulic characteristics, increase the total cost of pavement, and negatively affect the environment. In this paper, PA mixtures were produced with 5 different types of additives including 4 fibers and 1 filler. Their performances were compared with the reference mixtures containing virgin bitumen and polymer-modified bitumen. The performance of all mixes was assessed using: mechanical, hydraulic, economic, and environmental indicators. Then, the Delphi method was applied to compute the relative weights for the parameters in multi-criteria decision-making methods. Evaluation based on distance from average solution (EDAS), technique for order of the preference by similarity to ideal solution (TOPSIS), and weighted aggregated sum product assessment (WASPAS) were employed to rank the additives. According to the results obtained, aramid pulp displayed comparable and, for some parameters such as abrasion resistance, even better performance than polymer-modified bitumen, whereas cellulose fiber demonstrated the best performance regarding sustainability, due to economic and environmental benefits.

scholarly journals Methodology for Determining the Location of River Ports on a Modernized Waterway Based on Non-Cost Criteria: A Case Study of the Odra River Waterway

2021 ◽  
Vol 13 (6) ◽  
pp. 3571
Author(s):  
Bogusz Wiśnicki ◽  
Dorota Dybkowska-Stefek ◽  
Justyna Relisko-Rybak ◽  
Łukasz Kolanda
Keyword(s):  
Large Scale ◽  
Multicriteria Decision Making ◽  
Research Process ◽  
Optimal Selection ◽  
Investment Projects ◽  
Odra River ◽  
Research Problems ◽  
Selection Of ◽  
Construction Works

The paper responds to research problems related to the implementation of large-scale investment projects in waterways in Europe. As part of design and construction works, it is necessary to indicate river ports that play a major role within the European transport network as intermodal nodes. This entails a number of challenges, the cardinal one being the optimal selection of port locations, taking into account the new transport, economic, and geopolitical situation that will be brought about by modernized waterways. The aim of the paper was to present an original methodology for determining port locations for modernized waterways based on non-cost criteria, as an extended multicriteria decision-making method (MCDM) and employing GIS (Geographic Information System)-based tools for spatial analysis. The methodology was designed to be applicable to the varying conditions of a river’s hydroengineering structures (free-flowing river, canalized river, and canals) and adjustable to the requirements posed by intermodal supply chains. The method was applied to study the Odra River Waterway, which allowed the formulation of recommendations regarding the application of the method in the case of different river sections at every stage of the research process.

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