scholarly journals In Vitro Bioaccessibility and Antioxidant Activity of Polyphenolic Compounds from Spent Coffee Grounds-Enriched Cookies

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1837
Author(s):  
Luigi Castaldo ◽  
Sonia Lombardi ◽  
Anna Gaspari ◽  
Mario Rubino ◽  
Luana Izzo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Resolution Mass Spectrometry ◽  
Biological Properties ◽  
Bioactive Molecules ◽  
Mass Spectrometry Analysis ◽  
Total Phenolic ◽  
Spectrometry Analysis ◽  
Spent Coffee Ground ◽  
In Vitro Bioaccessibility

Spent coffee ground (SCG) is a significant by-product generated by the coffee industry. It is considered a great source of bioactive molecules well-recognized for exerting biological properties. This study aimed to implement SCG in a baked foods, such as cookies (SCGc), to increase their bioactive potential. A comprehensive study of the polyphenolic fraction of the SCG and SCGc using a high-resolution mass spectrometry analysis was performed. Moreover, the polyphenol bioaccessibility and change in antioxidant activity during simulated gastrointestinal digestion (GiD) were assessed. Data showed that SCGc provided 780 mg of melanoidins, 16.2 mg of chlorogenic acid (CGA), 6.5 mg of caffeine, and 0.08 mg of phenolic acids per 100 g of sample. Moreover, the 5-caffeoylquinic acid was the most relevant CGA found in SCG (116.4 mg/100 g) and SCGc (8.2 mg/100 g) samples. The antioxidant activity evaluated through three spectrophotometric tests, and the total phenolic compounds of SCGc samples exhibited significantly higher values than the control samples. Furthermore, during simulated GiD, the highest bioaccessibility of SCGc polyphenols was observed after the colonic stage, suggesting their potential advantages for human health. Therefore, SCG with high content in bioactive molecules could represent an innovative ingredient intended to fortify baked food formulations.

scholarly journals Chemical Composition, In Vitro Bioaccessibility and Antioxidant Activity of Polyphenolic Compounds from Nutraceutical Fennel Waste Extract

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1968
Author(s):  
Luigi Castaldo ◽  
Luana Izzo ◽  
Stefania De Pascale ◽  
Alfonso Narváez ◽  
Yelko Rodriguez-Carrasco ◽  
...  
Keyword(s):  
High Resolution Mass Spectrometry ◽  
Bioactive Molecules ◽  
Total Phenolic ◽  
Gastrointestinal Digestion ◽  
Foeniculum Vulgare ◽  
Dietary Polyphenols ◽  
In Vitro Bioaccessibility ◽  
Orbitrap Analysis ◽  
Waste Extract

Fennel (Foeniculum vulgare Mill.) waste contains a broad range of bioactive molecules, including polyphenols, which have poor bioaccessibility during gastrointestinal digestion. This work aimed to investigate the bioaccessibility of total phenolic compounds and the antioxidant capacity during simulated gastrointestinal digestion using two nutraceutical formulations based on non-acid-resistant (NAR) and acid-resistant (AR) capsules containing aqueous-based extracts from fennel waste. Moreover, to obtain a comprehensive investigation of the polyphenolic constituents of the fennel waste extract, a high-resolution mass spectrometry (Q-Orbitrap) analysis was performed. Notably, chlorogenic acids, such as 4-caffeoylquinic acid and 3,4-dicaffeoylquinic acid, were the most detected compounds found in assayed samples (1.949 and 0.490 mg/g, respectively). After in vitro gastrointestinal digestion, the extract contained in AR capsules displayed higher bioaccessibility in both the duodenal and colonic stages (1.96 and 5.19 mg GAE/g, respectively) than NAR capsules (1.72 and 3.50 mg GAE/g, respectively), suggesting that the acidic gastric conditions negatively affected the polyphenol compounds released from the NAR capsules. Therefore, the aqueous extract of fennel waste could be proposed as an innovative and easily available source of dietary polyphenols. Furthermore, the use of an AR capsule could improve the polyphenol bioaccessibility and can be proposed as a nutraceutical formulation.

scholarly journals Chemical Characterization and Biological Properties of Royal Jelly Samples From the Mediterranean Area

2020 ◽  
Vol 15 (2) ◽  
pp. 1934578X2090808 ◽  
Author(s):  
Soukaïna El-Guendouz ◽  
Alexandra M. Machado ◽  
Smail Aazza ◽  
Badiaâ Lyoussi ◽  
Maria G. Miguel ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Total Protein ◽  
Royal Jelly ◽  
Chemical Characterization ◽  
Biological Properties ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Decenoic Acid ◽  
Spectrometry Analysis

Royal jelly (RJ) is a bee product that has high nutritional value and is beneficial for the human health, earning importance as a functional food. Thus, the characterization of its main biological properties is with high importance. In this work, 6 RJ samples obtained in Morocco, Portugal, and Spain were evaluated in terms of total phenol and flavone/flavonol contents; total protein; 10-hydroxy-2-decenoic acid (10-HDA); volatiles composition; antioxidant and anti-inflammatory properties; and inhibition of tyrosinase, xanthine oxidase (XO), and acetylcholinesterase (AChE) activities. Total phenolic content ranged from 3 to 9 mg gallic acid equivalent/g RJ, and flavone/flavonol content from 0.1 to 0.5 mg quercetin equivalent/g RJ. 10-Hydroxy-2-decenoic acid content varied from 0.9% to 1.2% and total protein from 5.5% to 29.7%. Gas chromatography-flame ionization detector and gas chromatography-mass spectrometry analysis showed RJ volatiles dominated by linolenic acid, 2-decenoic acid, and octanoic acid in variable amounts. The antioxidant activity was monitored through nitric oxide (NO) scavenging activity and hydrogen peroxide (H2O2) scavenging capacity, where the IC50 ranged from 2.3 to 3.4 and 0.2 to 1.5 mg/mL, respectively. Anti-AChE activity IC50 ranged from 0.7 to 4.6 mg/mL, while XO inhibition IC50 ranged from 3.3 to 11.9 mg/mL. The results showed that phenols and flavonoids highly contributed to the RJ biological properties in contrast to 10-HDA and proteins.

scholarly journals Morphological, Pathogenic and Toxigenic Variability in the Rice Sheath Rot Pathogen Sarocladium Oryzae

Toxins ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 109 ◽  
Author(s):  
Kaat José Peeters ◽  
Ashley Haeck ◽  
Lies Harinck ◽  
Oluwatoyin Oluwakemi Afolabi ◽  
Kristof Demeestere ◽  
...  
Keyword(s):  
Acid Production ◽  
High Resolution Mass Spectrometry ◽  
Toxin Production ◽  
Mass Spectrometry Analysis ◽  
In Planta ◽  
Spectrometry Analysis ◽  
Helvolic Acid ◽  
Phylogenetic Lineages ◽  
Sheath Rot

Sheath rot is an emerging rice disease that leads to considerable yield losses. The main causal agent is the fungus Sarocladium oryzae. This pathogen is known to produce the toxins cerulenin and helvolic acid, but their role in pathogenicity has not been clearly established. S. oryzea isolates from different rice-producing regions can be grouped into three phylogenetic lineages. When grown in vitro, isolates from these lineages differed in growth rate, colour and in the ability to form sectors. A diverse selection of isolates from Rwanda and Nigeria, representing these lineages, were used to further study their pathogenicity and toxin production. Liquid chromatography high-resolution mass spectrometry analysis was used to measure cerulenin and helvolic acid production in vitro and in planta. The three lineages clearly differed in pathogenicity on the japonica cultivar Kitaake. Isolates from the least pathogenic lineage produced the highest levels of cerulenin in vitro. Helvolic acid production was not correlated with the lineage. Sectorisation was observed in isolates from the two least pathogenic lineages and resulted in a loss of helvolic acid production. In planta, only the production of helvolic acid, but not of cerulenin, correlated strongly with disease severity. The most pathogenic isolates all belonged to one lineage. They were phenotypically stable, shown by the lack of sectorisation, and therefore maintained high helvolic acid production in planta.

scholarly journals A New Flavone Glycoside From Lumnitzera littorea with In Vitro α-Glucosidase Inhibitory Activity

2019 ◽  
Vol 14 (6) ◽  
pp. 1934578X1985136
Author(s):  
Nguyen T. L. Thuy ◽  
Pham T. Thuy ◽  
Bui T. Tung ◽  
Huynh T. Loc ◽  
Truong T. T. Dang ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
High Resolution Mass Spectrometry ◽  
Positive Control ◽  
Mass Spectrometry Analysis ◽  
Two Dimensional ◽  
Flavone Glycoside ◽  
One Dimensional ◽  
Spectrometry Analysis ◽  
Resolution Mass

A new flavone glycoside, lumnitzerone (1), was isolated from leaves of Lumnitzera littorea, together with 9 known flavonoids. Their structures were elucidated by spectroscopic (one-dimensional, two-dimensional nuclear magnetic resonance) and high-resolution mass spectrometry analysis, and comparison with literature data. Extracts and all isolated compounds were evaluated for α-glucosidase inhibitory activity; all the extracts and most of the isolated compounds exhibited better activities than the positive control acarbose.

In vitro antioxidant and enzyme inhibitory properties and phenolic contents of crude extracts and fractions from different organs of the halophyte Lycium europaeum L.

2021 ◽  
Vol 17 ◽  
Author(s):  
Houaria Bendjedou ◽  
Malika Bennaceur ◽  
Houari Benamar ◽  
Maria João Rodrigues ◽  
Catarina Pereira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biological Properties ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Ic50 Values ◽  
Reducing Activity ◽  
Ethanol Extracts ◽  
High Level ◽  
Β Carotene

Background: Lycium europaeum L. is a medicinal and edible Mediterranean halophyte spiny shrub. However, studies regarding its biological properties focused mainly on its aerial organs. Objective: The objective of the present work was to make a comparative evaluation of the in vitro antioxidant and inhibitory enzyme activities of ethanol extracts and fractions (chloroform, ethyl acetate, n-butanol and aqueous) from roots and leaves of L. europaeum, along with its total phenolic, flavonoid and tannin contents. Methods: The antioxidant activity was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), superoxide radical, β-carotene bleaching, cupric reducing, and the ferric reducing activity methods. Results: The n-butanol fraction from roots had the highest antioxidant activity in all the assays. It was also the most active against acetylcholinesterase, butyrylcholinesterase, and urease (IC50 values 92.63, 118.26, and 135.60 µgmL-1, respectively). This fraction showed a high level of total phenolic, flavonoid, and tannin contents. Conclusion: The results suggest L. europaeum, especially its roots, as a candidate to be further explored as a source of bioactive products.

scholarly journals Chemical Constituents from Albiziae Cortex and Their Ability to Ameliorate Steatosis and Promote Proliferation and Anti-oxidation In Vitro

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4041
Author(s):  
Shi ◽  
Li ◽  
Cai ◽  
Liu ◽  
Li ◽  
...  
Keyword(s):  
High Resolution Mass Spectrometry ◽  
Umbilical Vein ◽  
Chemical Constituents ◽  
Mass Spectrometry Analysis ◽  
Cell Counting ◽  
Spectrometry Analysis ◽  
Albizzia Julibrissin ◽  
Lignan Glycosides ◽  
Reported Data

This study describes the chemical constituents of Albiziae Cortex and their ability to ameliorate steatosis and promote proliferation and anti-oxidation in vitro. Together, five known lignan glycosides, (7S,8R)-erythro-syringylglycerol-β–O-4′-sinapyl ether 9-O-β-D-glucopyranoside (1), (+)-lyoniresinol-9′-O-gluco-side (2), (−)-lyoniresinol-9′-O-glucoside (3), picraquassioside C (4), and icariside E5 (5), were isolated from the Albiziae Cortex. Their structures were elucidated by extensive NMR and high-resolution mass spectrometry analysis and compared with reported data. Oil Red O staining results revealed that compounds 1, 2, and 3 attenuated lipid accumulation and lipid metabolic disorders in FFAs (oleate/palmitate, 2:1 ratio, 0.3 mM)-exposed HepG2 cells. The Cell Counting Kit 8 (CCK-8) assay results revealed that compounds 1 and 5 can significantly promote human umbilical vein endothelial cell (HUVEC) proliferation; meanwhile, these compounds did not exhibit significant cytotoxicity against HUVECs. In addition, 2‘,7‘-dichlorofluorescein diacetate (DCFH-DA) staining results revealed that high glucose (HG)-induced reactive oxygen species (ROS) production was abolished by compounds 1, 2, and 3. This is the first report of the isolation of lignan skeletons from the genus Albizzia julibrissin with the ability to ameliorate steatosis and promote proliferation and anti-oxidation activities.

scholarly journals Assessment of antioxidant and antibacterial activity of Phoenix dactylifera L. seed extracts: Perspective for the development of new foods

2020 ◽  
Vol 4 (08) ◽  
pp. 298-308
Author(s):  
Lidia Ait Ouahioune ◽  
Fatma Bara ◽  
Karim Bariz ◽  
Karim Houali ◽  
Djamel Djenane
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Colorimetric Method ◽  
Phoenix Dactylifera ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phoenix Dactylifera L ◽  
Dpph Method

Background: Conventional food additives were associated with potential harm. Palm seeds are an interesting source of high-added value bioactive compounds, regarding their potential use in food industries. Aim: Date seeds extract (DSE) of Algerian Phoenix dactyliefra L. was studied for its phytochemical, antioxidant, and antibacterial activity and to identify its potential uses in foods. Material and methods: A total soluble solid of DSE (°Brix) was determined by refractometry. The total phenolic contents (TPCs) were determined according to the Folin-Cioclateu method and the total flavonoid and flavonols were also estimated with the colorimetric method. The DPPH method was used to determine the antioxidant activity of DSE. The IC50 values were also calculated and the antioxidant activity index (AAI) was determined. The agar well diffusion method was employed for the antibacterial activity of the aqueous DSE against various pathogens. Results: Moisture, ash, and fat contents (%) determined were 11.06, 1.33, and 7.06, respectively. The physicochemical analysis of the extract showed a pH value of 4.67, titratable acidity value of 0.64 citric acid/100 g, °Brix value = 3%, sugars values of 0.12, 0.47, and 0.35 g/L for reducing sugar, total sugar, and sucrose, respectively. The determination of phenolic compounds was carried out on three groups, namely total phenols = 229.67 mg GAEq/g, flavonoids = 201.12 mg QEq/g, and flavonols = 173.03 mg QEq/g, respectively. The DSE demonstrates to be effective as an antioxidant “in vitro” and showed a strong antibacterial activity with different inhibition levels, depending on the bacterial strains. Conclusion: The current investigations present knowledge about the possible role of palm industry by-products as a novel perspective for the development of new foods. We suggest that date seeds can be exploited in some food applications utilizing their high levels of bioactive molecules. Keywords: Phoenix dactylifera L., Degla-Baïda, seeds extract, bioactive molecules, biological activity, in vitro, novel perspective.

scholarly journals In Vitro Assessment of Organic and Residual Fractions of Nematicidal Culture Filtrates from Thirteen Tropical Trichoderma Strains and Metabolic Profiles of Most-Active

2022 ◽  
Vol 8 (1) ◽  
pp. 82
Author(s):  
Felicia Amalia Moo-Koh ◽  
Jairo Cristóbal-Alejo ◽  
María Fé Andrés ◽  
Jesús Martín ◽  
Fernando Reyes ◽  
...  
Keyword(s):  
High Resolution Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Array Detector ◽  
Diode Array Detector ◽  
Trichoderma Species ◽  
Potato Dextrose Broth ◽  
Spectrometry Analysis ◽  
Second Stage ◽  
In Vitro Assessment

The nematicidal properties of Trichoderma species have potential for developing safer biocontrol agents. In the present study, 13 native Trichoderma strains from T. citrinoviride, T. ghanense (2 strains), T. harzianum (4), T. koningiopsis, T. simmonsii, and T. virens (4) with nematicidal activity were selected and cultured in potato dextrose broth to obtain a culture filtrate (CF) for each. Each CF was partitioned with ethyl acetate to obtain organic (EA) and residual filtrate (RF) fractions, which were then tested on second-stage juveniles (J2s) of the nematodes Meloidogyne javanica and M. incognita in a microdilution assay. The most lethal strains were T. harzianum Th43-14, T. koningiopsis Th41-11, T. ghanense Th02-04, and T. virens Th32-09, which caused 51–100% mortality (%M) of J2s of both nematodes, mainly due to their RF fractions. Liquid chromatography–diode array detector-electrospray-high resolution mass spectrometry analysis of the most-active fractions revealed sesquiterpene and polyketide-like metabolites produced by the four active strains. These native Trichoderma strains have a high potential to develop safer natural products for the biocontrol of Meloidogyne species.

scholarly journals Assessment of antioxidant and antibacterial activity of Phoenix dactylifera L. seed extracts: Perspective for the development of new foods

2020 ◽  
Vol 4 (8) ◽  
pp. 298-308
Author(s):  
Lidia Ait Ouahioune ◽  
Fatma Bara ◽  
Karim Bariz ◽  
Karim Houali ◽  
Djamel Djenane
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Colorimetric Method ◽  
Phoenix Dactylifera ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phoenix Dactylifera L ◽  
Dpph Method

Background: Conventional food additives were associated with potential harm. Palm seeds are an interesting source of high-added value bioactive compounds, regarding their potential use in food industries. Aim: Date seeds extract (DSE) of Algerian Phoenix dactyliefra L. was studied for its phytochemical, antioxidant, and antibacterial activity and to identify its potential uses in foods. Material and methods: A total soluble solid of DSE (°Brix) was determined by refractometry. The total phenolic contents (TPCs) were determined according to the Folin-Cioclateu method and the total flavonoid and flavonols were also estimated with colorimetric method. The DPPH method was used to determine the antioxidant activity of DSE. The IC50 values were also calculated and the antioxidant activity index (AAI) was determined. The agar well diffusion method was employed for the antibacterial activity of the aqueous DSE against various pathogens. Results: Moisture, ash, and fat contents (%) determined were 11.06, 1.33, and 7.06, respectively. The physicochemical analysis of the extract showed a pH value of 4.67, titratable acidity value of 0.64 citric acid/100 g, °Brix value = 3%, sugars values of 0.12, 0.47, and 0.35 g/L for reducing sugar, total sugar, and sucrose, respectively. The determination of phenolic compounds was carried out on three groups, namely total phenols = 229.67 mg GAEq/g, flavonoids = 201.12 mg QEq/g, and flavonols = 173.03 mg QEq/g, respectively. The DSE demonstrates to be effective as an antioxidant “in vitro” and showed a strong antibacterial activity with different inhibition levels, depending on the bacterial strains. Conclusion: The current investigations present knowledge about the possible role of palm industry by-products as a novel perspective for the development of new foods. We suggest that date seeds can be exploited in some food applications utilizing their high levels of bioactive molecules. ​Keywords: Phoenix dactylifera L., Degla-Baïda, seeds extract, bioactive molecules, biological activity, in vitro, novel perspective.

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