scholarly journals Technological Potential of a Lupin Protein Concentrate as a Nutraceutical Delivery System in Baked Cookies

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1929
Author(s):  
Joana Mota ◽  
Ana Lima ◽  
Ricardo B. Ferreira ◽  
Anabela Raymundo
Keyword(s):  
Inhibitory Activity ◽  
Gastrointestinal Diseases ◽  
Strong Interactions ◽  
Protein Concentrate ◽  
Cancer Migration ◽  
Lupin Protein ◽  
Technological Potential ◽  
Efficient Delivery ◽  
Concomitant Reduction ◽  
Good Vehicle

Previous reports have shown that lupin protein extracts (LE) contain a polypeptide named deflamin with a potent matrix metalloproteinase (MMP)-9 inhibitory activity. The aim of our study was to develop an efficient delivery method for incorporating deflamin into cookies using different alternative flours. A lupin protein concentrate (10 g protein/100 g cookie dough) was added to gluten and gluten-free flours to produce savoury cookies, and its impacts on the physical properties of doughs and cookies, as well on the maintenance of deflamin’s anti-MMP-9 activity, were analysed. The results showed that the biochemical compositions of all cookies with LE presented higher protein and ash contents when compared to the control cookies. Rice, buckwheat and oat doughs were firmer than the others, whereas the addition of LE to kamut and buckwheat flours made cookies significantly firmer than the controls. Additionally, strong interactions between LE and several flours were observed, yielding different impacts on the MMP-9 bioactivity. Overall, the only flour that did not interfere with the desired nutraceutical activities was buckwheat, with 60% MMP-9 inhibitory activity and a concomitant reduction of colon cancer migration; hence, buckwheat flour was revealed to be a good vehicle to deliver bioactive deflamin, showing strong potential as a functional food to be used in preventive or curative approaches to gastrointestinal diseases.

Use of Different Proteases to Obtain Whey Protein Concentrate Hydrolysates with Inhibitory Activity toward Angiotensin-Converting Enzyme

2013 ◽  
Vol 38 (1) ◽  
pp. 102-109 ◽  
Author(s):  
Harriman A. Morais ◽  
Marialice P.C. Silvestre ◽  
Larissa L. Amorin ◽  
Viviane D.M. Silva ◽  
Mauro R. Silva ◽  
...  
Keyword(s):  
Angiotensin Converting Enzyme ◽  
Whey Protein ◽  
Inhibitory Activity ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Converting Enzyme

Identification and α-Glucosidase Inhibitory Activity of Meroterpenoids from Hericium erinaceus

Planta Medica ◽  
2020 ◽  
Vol 86 (08) ◽  
pp. 571-578
Author(s):  
Baosong Chen ◽  
Junjie Han ◽  
Li Bao ◽  
Wenzhao Wang ◽  
Ke Ma ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Inhibitory Activity ◽  
Gastrointestinal Diseases ◽  
Medicinal Mushroom ◽  
Chemical Derivatization ◽  
Eastern Asia ◽  
Hericium Erinaceus ◽  
Treatment Of Diabetes ◽  
Inhibitory Activities ◽  
New Compounds

Abstract Hericium erinaceus is a very popular edible and medicinal mushroom used for the treatment of enervation and gastrointestinal diseases in Eastern Asia. Chemical investigation on the fruiting body of Hericium erinaceus led to the isolation of 4 new (1 – 4) and 10 known meroterpenoids (5 – 14). The structures of new compounds were determined via analysis of NMR and MS data in combination with chemical derivatization. The inhibitory activities of 1 – 14 against α-glucosidase were evaluated using p-nitrophenyl-α-D-glucopyranoside, sucrose, or maltose as substrate. Compounds 6, 9, 11 – 13 were demonstrated to show the α-glucosidase inhibitory activities. This work confirms the potential of H. erinaceus in the treatment of diabetes.

scholarly journals Angiotensin Converting Enzyme Inhibitory Activity of Blue Lupin (Lupinus angustifolius) Proteins

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 492-492
Author(s):  
Chin Yee ◽  
Chew Yee ◽  
Amin Ismail ◽  
Toh Theng ◽  
Azrina Azlan
Keyword(s):  
Inhibitory Activity ◽  
Extraction Procedure ◽  
Protein Hydrolysates ◽  
Water Soluble ◽  
Lupin Protein ◽  
Significant Difference ◽  
Ic50 Values ◽  
Inhibitory Activities ◽  
Ace Activity ◽  
Ace Inhibitory

Abstract Objectives To evaluate the ACE inhibitory activity of protein hydrolysates derived from lupin Methods Methods used are spectrophotometer to assay the ACE activity, electrophoresis for estimation of protein molecular weight, and HPLC for determining products of ACE activity. Results The results revealed that the lupin flour was abundant in protein (43.3 g/100 g). Results from sequential Osborne extraction procedure showed that lupin protein comprised of 46% salt-soluble globulin, 27% water-soluble albumin, 18% alkaline-soluble glutelin and 7% alcohol-soluble prolamin fractions. Furthermore, lupin protein was rich in lysine but limiting in methionine. Hydrolysates prepared using Alcalase exhibited higher ACE inhibitory activities compared with those prepared using Flavourzyme, showing IC50 values ranging from 0.10 to 0.21 mg/mL. However, there was no significant difference in IC50 values between the hydrolysates prepared using Alcalase for 4, 10 and 16 h of hydrolysis times. Conclusions The results suggested that globulin was the major contributing protein fraction on ACE inhibitory effect in lupin protein. The lupin protein hydrolysates with potent ACE inhibitory activities can be incorporated into the daily diet to prevent hypertension. Funding Sources Ministry of Education Malaysia.

scholarly journals Synthesis and Evaluation of the Acetylcholinesterase Inhibitory Activities of Some Flavonoids Derived from Naringenin

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
The-Huan Tran ◽  
Thi-Thu-Hien Vo ◽  
Thi-Quynh-Nhi Vo ◽  
Thi-Cam-Nhung Cao ◽  
Thai-Son Tran
Keyword(s):  
Inhibitory Activity ◽  
Biological Activities ◽  
Developed Countries ◽  
Catalytic Triad ◽  
Peripheral Region ◽  
Strong Interactions ◽  
Research Approach ◽  
Aging Populations ◽  
Ache Inhibitory Activity ◽  
Long Time

Alzheimer’s disease (AD) is an irreversible neurodegenerative disease that affects many older people adversely. AD has been putting a huge socioeconomic burden on the healthcare systems of many developed countries with aging populations. The need for new therapies that can halt or reverse the progression of the disease is now extremely great. A research approach in the finding new treatment for AD that has attracted much interest from scientists for a long time is the reestablishment of cholinergic transmission through inhibition of acetylcholinesterase (AChE). Naringenin is a flavonoid with the potential inhibitory activity against AChE. From naringenin, many other flavonoid derivatives, such as flavanones and chalcones, can be synthesized. In this study, by applying the Williamson method, nine flavonoid derivatives were synthesized, including four flavanones and five chalcones. The evaluation of AChE inhibitory activity by the Ellman method showed that there were four substances (2, 4, 5, and 7) with relatively good biological activities (IC50 < 100 μM), and these biological activities were better than that of naringenin. The molecular docking revealed that strong interactions with amino acid residue Ser200 of the catalytic triad and those of the peripheral region of the enzyme were crucial for strong effects against AChE. Compound 7 had the strongest AChE inhibitory activity (IC50 13.0 ± 1.9 μM). This substance could be used for further studies.

200kv superconducting cryo electron microscope

1987 ◽  
Vol 45 ◽  
pp. 642-643
Author(s):  
M. Iwatsuki ◽  
Y. Kokubo ◽  
Y. Harada ◽  
J. Lehman
Keyword(s):  
Electron Microscope ◽  
Structure Analysis ◽  
Organic Materials ◽  
Strong Interactions ◽  
Specimen Preparation ◽  
Great Effort ◽  
Electron Microscopic ◽  
Crystal Fields ◽  
Special Skill ◽  
Long Time

In recent years, the electron microscope has been significantly improved in resolution and we can obtain routinely atomic-level high resolution images without any special skill. With this improvement, the structure analysis of organic materials has become one of the interesting targets in the biological and polymer crystal fields.Up to now, X-ray structure analysis has been mainly used for such materials. With this method, however, great effort and a long time are required for specimen preparation because of the need for larger crystals. This method can analyze average crystal structure but is insufficient for interpreting it on the atomic or molecular level. The electron microscopic method for organic materials has not only the advantage of specimen preparation but also the capability of providing various information from extremely small specimen regions, using strong interactions between electrons and the substance. On the other hand, however, this strong interaction has a big disadvantage in high radiation damage.

New form of Transmission Cross Coefficient for High-Resolution Imaging

1990 ◽  
Vol 48 (1) ◽  
pp. 60-61
Author(s):  
Kazuo Ishizuka
Keyword(s):  
Electron Microscope ◽  
High Resolution ◽  
Transmission Function ◽  
Incident Beam ◽  
Optical Microscope ◽  
Strong Interactions ◽  
Small Range ◽  
Specimen Thickness ◽  
Beam Direction ◽  
Diffracted Waves

It is well known that taking into account spacial and temporal coherency of illumination as well as the wave aberration is important to interpret an image of a high-resolution electron microscope (HREM). This occues, because coherency of incident electrons restricts transmission of image information. Due to its large spherical and chromatic aberrations, the electron microscope requires higher coherency than the optical microscope. On an application of HREM for a strong scattering object, we have to estimate the contribution of the interference between the diffracted waves on an image formation. The contribution of each pair of diffracted waves may be properly represented by the transmission cross coefficients (TCC) between these waves. In this report, we will show an improved form of the TCC including second order derivatives, and compare it with the first order TCC.In the electron microscope the specimen is illuminated by quasi monochromatic electrons having a small range of illumination directions. Thus, the image intensity for each energy and each incident direction should be summed to give an intensity to be observed. However, this is a time consuming process, if the ranges of incident energy and/or illumination direction are large. To avoid this difficulty, we can use the TCC by assuming that a transmission function of the specimen does not depend on the incident beam direction. This is not always true, because dynamical scattering is important owing to strong interactions of electrons with the specimen. However, in the case of HREM, both the specimen thickness and the illumination angle should be small. Therefore we may neglect the dependency of the transmission function on the incident beam direction.

Inhibitory effects of bioaccessible anthocyanins and procyanidins from apple, red grape, cinnamon on α-amylase, α-glucosidase and lipase

2020 ◽  
pp. 1-9
Author(s):  
Pınar Ercan ◽  
Sedef Nehir El
Keyword(s):  
Inhibitory Activity ◽  
Digestive Enzymes ◽  
Positive Control ◽  
Anthocyanin Content ◽  
Inhibitory Effects ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Before And After ◽  
Red Grape

Abstract. The goals of this study were to determine and evaluate the bioaccessibility of total anthocyanin and procyanidin in apple (Amasya, Malus communis), red grape (Papazkarası, Vitis vinifera) and cinnamon (Cassia, Cinnamomum) using an in vitro static digestion system based on human gastrointestinal physiologically relevant conditions. Also, in vitro inhibitory effects of these foods on lipid (lipase) and carbohydrate digestive enzymes (α-amylase and α-glucosidase) were performed with before and after digested samples using acarbose and methylumbelliferyl oleate (4MUO) as the positive control. While the highest total anthocyanin content was found in red grape (164 ± 2.51 mg/100 g), the highest procyanidin content was found in cinnamon (6432 ± 177.31 mg/100 g) (p < 0.05). The anthocyanin bioaccessibilities were found as 10.2 ± 1%, 8.23 ± 0.64%, and 8.73 ± 0.70% in apple, red grape, and cinnamon, respectively. The procyanidin bioaccessibilities of apple, red grape, and cinnamon were found as 17.57 ± 0.71%, 14.08 ± 0.74% and 18.75 ± 1.49%, respectively. The analyzed apple, red grape and cinnamon showed the inhibitory activity against α-glucosidase (IC50 544 ± 21.94, 445 ± 15.67, 1592 ± 17.58 μg/mL, respectively), α-amylase (IC50 38.4 ± 7.26, 56.1 ± 3.60, 3.54 ± 0.86 μg/mL, respectively), and lipase (IC50 52.7 ± 2.05, 581 ± 54.14, 49.6 ± 2.72 μg/mL), respectively. According to our results apple, red grape and cinnamon have potential to inhibit of lipase, α-amylase and α-glucosidase digestive enzymes.

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