scholarly journals RSM Optimization for the Recovery of Technofunctional Protein Extracts from Porcine Hearts

Foods ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1733
Author(s):  
Dolors Parés ◽  
Mònica Toldrà ◽  
Estel Camps ◽  
Juan Geli ◽  
Elena Saguer ◽  
...  
Keyword(s):  
Salt Content ◽  
Physicochemical Characteristics ◽  
Protein Solutions ◽  
Process Conditions ◽  
Foaming Properties ◽  
Blood Proteins ◽  
Molecular Weights ◽  
Extraction Buffer ◽  
High Salt Content ◽  
Rsm Optimization

Meat byproducts, such as the internal organs from slaughtered animals, are usually underutilized materials with low commercial value. The functional (emulsifying, gelling, and foaming) properties of soluble protein extracts derived from pork hearts were investigated, as well as their molecular weight distribution. A central composite design (CCD) for two process variables (pH and ionic strength of the extraction buffer) was used to foreknow the effects of the process conditions on the physicochemical characteristics and technofunctionality of the protein extracts by means of the response surface methodology (RSM). SDS-PAGE patterns of the heart protein solutions revealed multiple bands with molecular weights ranging from 15 to 220 kDa, mainly corresponding to sarcoplasmic, myofibrillar, as well as blood proteins. The best extraction conditions to obtain protein fractions with good foaming properties would correspond to acid pH (pH ≤ 5) and high salt content (2–4%). On the contrary, solutions recovered at pH > 5 with low NaCl contents were the ones showing better emulsifying properties. Regarding gelation ability, heat-induced gels were obtained from extracts at pH 6.5–8, which showed improved firmness with increasing NaCl content (2–4%). Satisfactory second-order polynomial models were obtained for all the studied response variables, which can be useful in guiding the development of functional ingredients tailored for specific uses to maximize applications.

Effect of Sodium Nitrite on Growth of Shigella flexneri

1991 ◽  
Vol 54 (6) ◽  
pp. 424-428 ◽  
Author(s):  
LAURA L. ZAIKA ◽  
ANNA H. KIM ◽  
LOUISE FORD
Keyword(s):  
Exponential Growth ◽  
Shigella Flexneri ◽  
Salt Content ◽  
Brain Heart Infusion ◽  
Growth Curves ◽  
Inhibitory Effect ◽  
Plate Count ◽  
Generation Times ◽  
High Salt Content ◽  
Lag Times

A partial factorial design study of the effect of NaNO2 (0, 100, 200, 1000 ppm) in combination with NaCl (0.5, 2.5, 4.0%), pH (7.5, 6.5, 5.5), and temperature (37, 28, 19°C) on growth of Shigella flexneri is reported. Experiments were done aerobically in brain-heart infusion medium, using an inoculum of 1 × 103 CFU/ml. Growth curves were fitted from plate count data by the Gompertz equation; exponential growth rates, lag times, generation times, and maximum populations were derived for all variable combinations. In the absence of nitrite, the organism grew well under all test conditions at 37 and 28°C but did not grow at 19°C at pH 5.5 nor at pH 7.5 with 4% NaCl. Nitrite did not affect growth in media of pH 7.5 at 37 and 28°C. At pH 6.5 growth was inhibited by 1000 ppm NaNO2. The organism failed to grow at 19°C at all nitrite levels in the presence of 2.5 or 4.0% NaCl. The inhibitory effect of nitrite was much greater in media of pH 5.5 and increased with increasing salt levels. More inhibition was apparent at 28 than at 37°C. While lack of growth was used as a paradigm of the effect of nitrite on S. flexneri, nitrite also increased the lag and generation times and decreased the exponential growth rate. Results indicated that NaNO2 in combinations with low temperature, low pH, and high salt content can effectively inhibit the growth of S. flexneri.

Resource-saving Technologies and Some Proposals for the Creation of Automated Reclamation Systems

2021 ◽  
Vol 25 (7) ◽  
pp. 8-12
Author(s):  
Z.G. Lamerdonov ◽  
T.Yu. Khashirova ◽  
S.A. Zhaboev ◽  
L.Zh. Nastueva ◽  
A.А. Shogenov ◽  
...  
Keyword(s):  
Drip Irrigation ◽  
Salt Content ◽  
Experimental Studies ◽  
Soil Surface ◽  
Growing Season ◽  
Resource Saving ◽  
Water Savings ◽  
Irrigation Method ◽  
High Salt Content ◽  
Reclamation System

The results of experimental studies of the local subsurface irrigation method in comparison with drip irrigation carried out in the laboratory, which showed water savings due to a decrease in evaporation from the soil surface by 10–15 percent are presented. The method of irrigation in closed greenhouse farms using water with a high salt content is described. The paper proposes new patented schematic solutions for protecting plants from frost and pests, describes a multifunctional engineering and reclamation system capable of performing various operations depending on the emerging problems during the growing season.

scholarly journals Filter made of cuprammonium regenerated cellulose for virus removal: a mini-review

Cellulose ◽  
2021 ◽  
Author(s):  
Shoichi Ide
Keyword(s):  
Membrane Filtration ◽  
Hollow Fiber Membrane ◽  
Membrane Filter ◽  
Three Dimensional ◽  
Protein Solutions ◽  
Regenerated Cellulose ◽  
Cellulose Solution ◽  
Virus Removal ◽  
Biological Products ◽  
Molecular Weights

AbstractIn 1989, Asahi Kasei commercialized a porous hollow fiber membrane filter (Planova™) made of cuprammonium regenerated cellulose, making it possible for the first time in the world to “remove viruses from protein solutions by membrane filtration”. Planova has demonstrated its usefulness in separating proteins and viruses. Filters that remove viruses from protein solutions, i.e., virus removal filters (VFs), have become one of the critical modern technologies to assure viral safety of biological products. It has also become an indispensable technology for the future. The performance characteristics of VFs can be summarized in two points: 1) the virus removal performance increases as the virus diameter increases, and 2) the recovery rate of proteins with molecular weights greater than 10,000 exceeds the practical level. This paper outlines the emergence of VF and its essential roles in the purification process of biological products, requirements for VF, phase separation studies for cuprammonium cellulose solution, comparison between Planova and other regenerated cellulose flat membranes made from other cellulose solutions, and the development of Planova. The superior properties of Planova can be attributed to its highly interconnected three-dimensional network structure. Furthermore, future trends in the VF field, the subject of this review, are discussed.

scholarly journals SUBSTANTIATION OF CHOICE OF VEGETABLE CROPS SUITABLE FOR GROWING ON SALTED SOILS

2020 ◽  
Vol 5 (4(73)) ◽  
pp. 12-18
Author(s):  
G.T. Bekmirzaev ◽  
I.A. Begmatov ◽  
D.B. Yulchiev
Keyword(s):  
Experimental Study ◽  
New Zealand ◽  
Salt Content ◽  
Vegetable Crops ◽  
Agricultural Systems ◽  
Salt Tolerant ◽  
As Species ◽  
High Salt Content ◽  
The University ◽  
Selection Of

The purpose of the experimental study was the selection of salt tolerant crops and the search for useful horticultural species for growing them on saline lands. The experimental study was conducted at the University of Algarve, Portugal, in a greenhouse. The following vegetable crops were selected for research: lettuce (Lactuca sativaL), New Zealand spinach (Tetragonia tetragonioides) and garden purslane (Portulaca oleracea). Experimental results showed that New Zealand spinach and garden purslane have high potential as species resistant to high salt content and are therefore recommended for cultivation in order to reduce soil salinity. The above crops, mainly New Zealandspinach, are good types of garden crops with high useful qualities and productivity. Therefore, it has been shown that this method is a clean and environmentally friendly tool to prevent salinization and maintain the sustainability of agricultural systems

scholarly journals Ham salting in plástic bags: A way to reduce salt use?

2018 ◽  
Vol 36 (2) ◽  
pp. 166-173
Author(s):  
Joel Girón ◽  
Raúl Grau ◽  
José Manuel Barat
Keyword(s):  
Sensory Analysis ◽  
Total Mass ◽  
Salt Content ◽  
Physicochemical Characteristics ◽  
Sensory Assessment ◽  
Salt Reduction ◽  
Plastic Bags ◽  
Dry Cured Ham ◽  
Plastic Bag

The objective of this work was to study salt reduction in the processing and final product of Iberian dry-cured ham manufacturing. For this purpose, high-barrier plastic bags were used in the salting stage, with salt doses of 2.5 and 3.2% at 10 and 15 d. The following aspects were evaluated: the production and salt content of brine, the analytical determinations of the hams after the salting stage, the changes in the total mass of the hams during processing and the analytical and sensory assessment of the dry-cured product. The experiment results showed that the plastic bag salting minimized the production of brine by 61 and 63% and reduced the salt content in the final product without altering the physicochemical characteristics or the hygienic quality of the dry-cured ham. In the sensory analysis, the samples salted with 2.5% doses were preferred by consumers.

The Effect of High Salinity Water to the Compressive Strength of Mine Backfill Material

2018 ◽  
Vol 777 ◽  
pp. 554-558
Author(s):  
Vu Hai Dang ◽  
Manoon Masniyom
Keyword(s):  
Compressive Strength ◽  
High Salinity ◽  
Saline Water ◽  
Salt Content ◽  
High Salt ◽  
Mine Backfill ◽  
High Salinity Water ◽  
Solid Ratio ◽  
High Salt Content ◽  
Salinity Water

The effect of the high salinity water on the compressive strength of mine backfill was studied. Two types of salinity water: saturated and unsaturated brines were employed to mix with mine backfill materials, and the results were compared. The one with saturated brine had high salt content of 400 g/l while the other had 200 g/l. The results showed that compressive strength decreased with increasing salt content. The mine backfill with high salt content (saturated brine) exhibited the poorest compressive strength in which its strength decreased to approximately 50-70 % of the original strength gained from the backfill samples based on water without salt. Additionally, the optimal saline water solid ratio was 0.2.

scholarly journals Agronomic characteristics of the Pennisetum glaucum submitted to water and saline stresses

2020 ◽  
Vol 43 ◽  
pp. e50468
Author(s):  
Mirna Clarissa Rodrigues de Almeida ◽  
Mauricio Luiz de Mello Vieira Leite ◽  
Luciana Sandra Bastos de Souza ◽  
Vicente José Laamon Pinto Simões ◽  
Luiz Guilherme Medeiros Pessoa ◽  
...  
Keyword(s):  
Arid Regions ◽  
Pennisetum Glaucum ◽  
Block Design ◽  
Salt Content ◽  
Morphological Characteristics ◽  
Saline Stress ◽  
Initial Growth ◽  
Randomized Block Design ◽  
High Salt Content ◽  
Four Levels

Agricultural production in semi-arid regions is limited due to water availability. In addition, the water quality available for irrigation is often compromised due to the high salt content present. Millet is a forage species considered tolerant to water deficit and moderately salt tolerant. In view of the above, the objective was to evaluate the growth of millet under water and saline stress associates. The experiment was carried out in a randomized block design, in a 4x3 factorial scheme, composed of four levels of water replacement, based on crop evapotranspiration (ETc): 25%.ETc, 50%.ETc, 75%.ETc and 100%.ETc and three levels of water salinity (0.03, 2.0 and 4.0 dS m-1). With 25%.ETc independent of salinity, all morphological characteristics of millet were affected, occurring death of plants in the initial growth phase. In the absence of salt and greater availability of water, greater plant growth occurred. With respect to salinity, there was a reduction in the increment of all variables evaluated, with the highest reduction at the highest saline level (4.0 dS m-1). Water and salt stresses, when associated, reduce the growth of millet, since concentrations above 2.0 dS m-1 and less than 50%.ETc compromise its full development, providing declines in yield.

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