scholarly journals Alginate NiFe2O4 Nanoparticles Cryogel for Electrochemical Glucose Biosensor Development

Gels ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 272
Author(s):  
Amin Fatoni ◽  
Aziz Wijonarko ◽  
Mekar Dwi Anggraeni ◽  
Dadan Hermawan ◽  
Hartiwi Diastuti ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Linear Response ◽  
Regression Line ◽  
Glucose Biosensor ◽  
Limit Of Quantification ◽  
Alginate Solution ◽  
Operational Conditions ◽  
Glucose Determination ◽  
Nife2o4 Nanoparticles ◽  
Fabrication Parameters

Glucose biosensors based on porous material of alginate cryogel has been developed, and the cryogel provides a large surface area for enzyme immobilization. The alginate cryogel has been supplemented with NiFe2O4 nanoparticles to improve the electron transfer for electrochemical detection. The fabrication parameters and operational conditions for the biosensor have also been optimized. The results showed that the optimum addition of NiFe2O4 nanoparticles to the alginate solution was 0.03 g/mL. The optimum operational conditions for the electrochemical detection were a cyclic voltammetry scan rate of 0.11 V/s, buffer pH of 7.0, and buffer concentration of 150 mM. The fabricated alginate NiFe2O4 nanoparticles cryogel-based glucose biosensor showed a linear response for glucose determination with a regression line of y = 18.18x + 455.28 and R² = 0.98. Furthermore, the calculated detection limit was 0.32 mM and the limit of quantification was 1.06 mM.

Amperometric glucose biosensor based on NiFe2O4 nanoparticles and chitosan

2010 ◽  
Vol 145 (1) ◽  
pp. 293-298 ◽  
Author(s):  
Liqiang Luo ◽  
Qiuxia Li ◽  
Yanhong Xu ◽  
Yaping Ding ◽  
Xia Wang ◽  
...  
Keyword(s):  
Glucose Biosensor ◽  
Nife2o4 Nanoparticles

scholarly journals Glucose Biosensor Based on Disposable Activated Carbon Electrodes Modified with Platinum Nanoparticles Electrodeposited on Poly(Azure A)

Sensors ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 4489 ◽  
Author(s):  
Francisco Jiménez-Fiérrez ◽  
María Isabel González-Sánchez ◽  
Rebeca Jiménez-Pérez ◽  
Jesús Iniesta ◽  
Edelmira Valero
Keyword(s):  
Platinum Nanoparticles ◽  
High Performance ◽  
Electrochemical Biosensor ◽  
Limit Of Detection ◽  
Electrochemical Measurement ◽  
Glucose Biosensor ◽  
Carbon Electrodes ◽  
Real Samples ◽  
Glucose Determination ◽  
Azure A

Herein, a novel electrochemical glucose biosensor based on glucose oxidase (GOx) immobilized on a surface containing platinum nanoparticles (PtNPs) electrodeposited on poly(Azure A) (PAA) previously electropolymerized on activated screen-printed carbon electrodes (GOx-PtNPs-PAA-aSPCEs) is reported. The resulting electrochemical biosensor was validated towards glucose oxidation in real samples and further electrochemical measurement associated with the generated H2O2. The electrochemical biosensor showed an excellent sensitivity (42.7 μA mM−1 cm−2), limit of detection (7.6 μM), linear range (20 μM–2.3 mM), and good selectivity towards glucose determination. Furthermore, and most importantly, the detection of glucose was performed at a low potential (0.2 V vs. Ag). The high performance of the electrochemical biosensor was explained through surface exploration using field emission SEM, XPS, and impedance measurements. The electrochemical biosensor was successfully applied to glucose quantification in several real samples (commercial juices and a plant cell culture medium), exhibiting a high accuracy when compared with a classical spectrophotometric method. This electrochemical biosensor can be easily prepared and opens up a good alternative in the development of new sensitive glucose sensors.

scholarly journals Commercially Fabricated Printed Circuit Board Sensing Electrodes for Biomarker Electrochemical Detection: The Importance of Electrode Surface Characteristics in Sensor Performance

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 741 ◽  
Author(s):  
Gorachand Dutta ◽  
Anna Regoutz ◽  
Despina Moschou
Keyword(s):  
Electrode Surface ◽  
Linear Response ◽  
Printed Circuit Board ◽  
Limit Of Detection ◽  
Surface Characteristics ◽  
Glucose Biosensor ◽  
Circuit Board ◽  
Printed Circuit ◽  
Sensor Performance ◽  
Immobilized Glucose Oxidase

Here we report the first PCB-implemented electrochemical glucose biosensor usingcovalently immobilized glucose oxidase (GOx) on the commercially fabricated PCB electrodesurface, taking particular care on the electrode surface characteristics and their effect on sensorperformance. Based on the results, this assay exhibits a highly linear response from 500 μM to 20mM (R = 0.9961) and a lower limit of detection of 500 μM.

scholarly journals Quantification of Urinary Albumin by Using Protein Cleavage and LC-MS/MS

2009 ◽  
Vol 55 (6) ◽  
pp. 1100-1107 ◽  
Author(s):  
Jesse C Seegmiller ◽  
David R Barnidge ◽  
Bradley E Burns ◽  
Timothy S Larson ◽  
John C Lieske ◽  
...  
Keyword(s):  
Calibration Curve ◽  
Dynamic Range ◽  
Linear Regression Analysis ◽  
Regression Line ◽  
Method Comparison ◽  
Internal Standard ◽  
Urinary Albumin ◽  
Protein Cleavage ◽  
Limit Of Quantification ◽  
External Calibration Curve

Abstract Background: Urinary albumin excretion is a sensitive diagnostic and prognostic marker for renal disease. Therefore, measurement of urinary albumin must be accurate and precise. We have developed a method to quantify intact urinary albumin with a low limit of quantification (LOQ). Methods: We constructed an external calibration curve using purified human serum albumin (HSA) added to a charcoal-stripped urine matrix. We then added an internal standard, 15N-labeled recombinant HSA (15NrHSA), to the calibrators, controls, and patient urine samples. The samples were reduced, alkylated, and digested with trypsin. The concentration of albumin in each sample was determined by liquid chromatography–tandem mass spectrometry (LC-MS/MS) and linear regression analysis, in which the relative abundance area ratio of the tryptic peptides 42LVNEVTEFAK51 and 526QTALVELVK534 from albumin and 15NrHSA were referenced to the calibration curve. Results: The lower limit of quantification was 3.13 mg/L, and the linear dynamic range was 3.13–200 mg/L. Replicate digests from low, medium, and high controls (n = 5) gave intraassay imprecision CVs of 2.8%–11.0% for the peptide 42LVNEVTEFAK51, and 1.9%–12.3% for the 526QTALVELVK534 peptide. Interassay imprecision of the controls for a period of 10 consecutive days (n = 10) yielded CVs of 1.5%–14.8% for the 42LVNEVTEFAK51 peptide, and 6.4%–14.1% for the 526QTALVELVK534 peptide. For the 42LVNEVTEFAK51 peptide, a method comparison between LC-MS/MS and an immunoturbidometric method for 138 patient samples gave an R2 value of 0.97 and a regression line of y = 0.99x + 23.16. Conclusions: Urinary albumin can be quantified by a protein cleavage LC-MS/MS method using a 15NrHSA internal standard. This method provides improved analytical performance in the clinically relevant range compared to a commercially available immunoturbidometric assay.

scholarly journals Reduced Graphene Oxide and Polyaniline Nanofibers Nanocomposite for the Development of an Amperometric Glucose Biosensor

Sensors ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 948
Author(s):  
Anton Popov ◽  
Ruta Aukstakojyte ◽  
Justina Gaidukevic ◽  
Viktorija Lisyte ◽  
Asta Kausaite-Minkstimiene ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Thermal Reduction ◽  
Glucose Biosensor ◽  
Practical Application ◽  
X Ray Diffraction ◽  
Glucose Determination ◽  
Reduced Graphene ◽  
Graphite Rod

The control of glucose concentration is a crucial factor in clinical diagnosis and the food industry. Electrochemical biosensors based on reduced graphene oxide (rGO) and conducting polymers have a high potential for practical application. A novel thermal reduction protocol of graphene oxide (GO) in the presence of malonic acid was applied for the synthesis of rGO. The rGO was characterized by scanning electron microscopy, X-ray diffraction analysis, Fourier-transform infrared spectroscopy, and Raman spectroscopy. rGO in combination with polyaniline (PANI), Nafion, and glucose oxidase (GOx) was used to develop an amperometric glucose biosensor. A graphite rod (GR) electrode premodified with a dispersion of PANI nanostructures and rGO, Nafion, and GOx was proposed as the working electrode of the biosensor. The optimal ratio of PANI and rGO in the dispersion used as a matrix for GOx immobilization was equal to 1:10. The developed glucose biosensor was characterized by a wide linear range (from 0.5 to 50 mM), low limit of detection (0.089 mM), good selectivity, reproducibility, and stability. Therefore, the developed biosensor is suitable for glucose determination in human serum. The PANI nanostructure and rGO dispersion is a promising material for the construction of electrochemical glucose biosensors.

scholarly journals 3D Hydrogen Titanate Nanotubes on Ti Foil: A Carrier for Enzymatic Glucose Biosensor

Sensors ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 1024 ◽  
Author(s):  
Lulu Ma ◽  
Zhao Yue ◽  
Guona Huo ◽  
Shasha Zhang ◽  
Baolin Zhu ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Linear Response ◽  
High Selectivity ◽  
3D Structure ◽  
Glucose Biosensor ◽  
Titanate Nanotubes ◽  
Cross Linking ◽  
In Situ Fabrication ◽  
Hydrogen Titanate

Glucose oxidase (GOx) based biosensors are commercialized and marketed for the high selectivity of GOx. Incorporation nanomaterials with GOx can increase the sensitivity performance. In this work, an enzyme glucose biosensor based on nanotubes was fabricated. By using Ti foil as a carrier, hydrogen titanate nanotubes (HTNTs), which present fine 3D structure with vast pores, were fabricated in-situ by the hydrothermal treatment. The multilayer nanotubes are open-ended with a diameter of 10 nm. Then glucose oxidase (GOx) was loaded on the nanotubes by cross-linking to form an electrode of the amperometric glucose biosensor (GOx/HTNTs/Ti electrode). The fabricated GOx/HTNTs/Ti electrode had a linear response to 1–10 mM glucose, and the response time was 1.5 s. The sensitivity of the biosensor was 1.541 μA·mM-1·cm-2, and the detection limit (S/N = 3) was 59 μM. Obtained results indicate that the in-situ fabrication and unique 3D structure of GOx/HTNTs/Ti electrode are beneficial for its sensitivity.

scholarly journals Continuous Flow Electrometric Glucose Determination

1972 ◽  
Vol 9 (1-6) ◽  
pp. 47-50 ◽  
Author(s):  
C. E. Wilde ◽  
P. Sewell
Keyword(s):  
Continuous Flow ◽  
Linear Response ◽  
Flow System ◽  
High Sensitivity ◽  
Glucose Determination ◽  
Time Limits ◽  
Continuous Flow System ◽  
Electrode Response ◽  
Nitrogen Ratio

A continuous flow system for the determination of glucose in blood or plasma using glucose oxidase is described. Oxygen uptake is measured by a Clarke electrode linked through an oxygen monitor and pH meter to a recorder. The omission of a peroxidase-chromogen step increases the specificity of the assay which gives very good correlation with the hexokinase-NADPH method. The system has a high sensitivity which can be adjusted by changing the oxygen-nitrogen ratio of the segmenting gas. It is useful when measuring ‘true glucose’ in low concentration and gives a linear response within the range 0 to 200 mg/100 ml. The electrode response time limits the rate of analysis to 30 samples per hour if adequate precision is to be maintained. The advantages of a closed continuous flow system over manual electrometric methods are discussed.

Hydrothermal synthesis of NiFe2O4 nanoparticles as an efficient electrocatalyst for the electrochemical detection of bisphenol A

2020 ◽  
Vol 44 (19) ◽  
pp. 7698-7707 ◽  
Author(s):  
Ganesh Kesavan ◽  
Nandini Nataraj ◽  
Shen-Ming Chen ◽  
Li-Heng Lin
Keyword(s):  
Hydrothermal Synthesis ◽  
Bisphenol A ◽  
Electrochemical Detection ◽  
Selective Detection ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Nife2o4 Nanoparticles ◽  
Screen Printed

In this study, the sensitive and selective detection of bisphenol A (BPA) was achieved using a screen-printed carbon electrode (NFO/SPCE) modified with hydrothermally synthesized NiFe2O4 nanoparticles.

scholarly journals Glucose Biosensor Based on Dendritic Gold Nanostructures Electrodeposited on Graphite Electrode by Different Electrochemical Methods

Chemosensors ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 188
Author(s):  
Almira Ramanaviciene ◽  
Natalija German ◽  
Asta Kausaite-Minkstimiene ◽  
Arunas Ramanavicius
Keyword(s):  
Glucose Oxidase ◽  
Limit Of Detection ◽  
Differential Pulse ◽  
Glucose Biosensor ◽  
Electrochemical Methods ◽  
Gold Nanostructures ◽  
Glucose Determination ◽  
One Step ◽  
Constant Potential ◽  
Good Repeatability

In this research, we have demonstrated a one-step electrochemical deposition of dendritic gold nanostructures (DGNs) on a graphite rod (GR) electrode without any template, seeds, surfactants, or stabilizers. Three electrochemical methods, namely, constant potential amperometry (CPA), pulse amperometry, and differential pulse voltammetry, were used for DGN synthesis on GR electrode and further application in enzymatic glucose biosensors. Formed gold nanostructures, including DGNs, were characterized by a field emission scanning electron microscopy. The optimal concentration of HAuCl4 (6.0 mmol L−1), duration of DGNs synthesis (400 s), electrodeposition potential (−0.4 V), and the best electrochemical method (CPA) were determined experimentally. Then the enzyme, glucose oxidase, was adsorbed on the surface of DGNs and covalently cross-linked with glutaraldehyde vapor. The enzymatic glucose biosensor based on DGNs electrodeposited at optimal conditions and modified with glucose oxidase showed a quick response (less than 3 s), a high saturation current (291 μA), appropriate linear range (up to 9.97 mmol L−1 of glucose, R2 = 0.9994), good repeatability (RSD 2.4, 2.2 and 1.5% for 2, 30, 97 mmol L−1 of glucose), low limit of detection (0.059 mmol L−1, S/N = 3) and good stability. Additionally, this biosensor could be successfully applied for glucose determination in real samples with good accuracy. These results proved the principle of enzymatic glucose biosensor development based on DGNs as the basis for further investigations.

Sign in / Sign up

Export Citation Format

Share Document