scholarly journals Photosensitizers Based on G-Quadruplex Ligand for Cancer Photodynamic Therapy

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1340
Author(s):  
Keiko Kawauchi ◽  
Ryoto Urano ◽  
Natsuki Kinoshita ◽  
Shin Kuwamoto ◽  
Takeru Torii ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Secondary Structure ◽  
Molecular Target ◽  
Untranslated Regions ◽  
Reactive Oxidative Species ◽  
Dna And Rna ◽  
Porphyrin Derivatives ◽  
G Quadruplex ◽  
Novel Strategy ◽  
Oxidative Species

G-quadruplex (G4) is the non-canonical secondary structure of DNA and RNA formed by guanine-rich sequences. G4-forming sequences are abundantly located in telomeric regions and in the promoter and untranslated regions (UTR) of cancer-related genes, such as RAS and MYC. Extensive research has suggested that G4 is a potential molecular target for cancer therapy. Here, we reviewed G4 ligands as photosensitizers for cancer photodynamic therapy (PDT), which is a minimally invasive therapeutic approach. The photosensitizers, such as porphyrins, were found to be highly toxic against cancer cells via the generation of reactive oxidative species (ROS) upon photo-irradiation. Several porphyrin derivatives and analogs, such as phthalocyanines, which can generate ROS upon photo-irradiation, have been reported to act as G4 ligands. Therefore, they have been implicated as promising photosensitizers that can selectively break down cancer-related DNA and RNA forming G4. In this review, we majorly focused on the potential application of G4 ligands as photosensitizers, which would provide a novel strategy for PDT, especially molecularly targeted PDT (mtPDT).

scholarly journals Silver sulfide nanoparticles for photodynamic therapy of human lymphoma cells via disruption of energy metabolism

RSC Advances ◽  
2019 ◽  
Vol 9 (51) ◽  
pp. 29936-29941 ◽  
Author(s):  
Ge Wang ◽  
Jing Liu ◽  
Lin Zhu ◽  
Yuming Guo ◽  
Lin Yang
Keyword(s):  
Photodynamic Therapy ◽  
Energy Metabolism ◽  
Silver Sulfide ◽  
Light Irradiation ◽  
Hybrid Nanoparticles ◽  
Reactive Oxidative Species ◽  
Lymphoma Cells ◽  
Human Lymphoma ◽  
Silver Sulfide Nanoparticles ◽  
Oxidative Species

Ag2S/BSA hybrid nanoparticles were prepared and studied for their ability to inhibit the growth of human lymphoma cells under light irradiation, via inducing the accumulation of intracellular reactive oxidative species to disrupt energy metabolism.

scholarly journals The i-Motif as a Molecular Target: More Than a Complementary DNA Secondary Structure

2021 ◽  
Vol 14 (2) ◽  
pp. 96
Author(s):  
Susie L. Brown ◽  
Samantha Kendrick
Keyword(s):  
Gene Expression ◽  
Secondary Structure ◽  
Biological Function ◽  
Molecular Target ◽  
Therapeutic Approaches ◽  
Promoter Regions ◽  
New Therapeutic Approaches ◽  
G Quadruplex ◽  
Dna Elements ◽  
Insight Into

Stretches of cytosine-rich DNA are capable of adopting a dynamic secondary structure, the i-motif. When within promoter regions, the i-motif has the potential to act as a molecular switch for controlling gene expression. However, i-motif structures in genomic areas of repetitive nucleotide sequences may play a role in facilitating or hindering expansion of these DNA elements. Despite research on the i-motif trailing behind the complementary G-quadruplex structure, recent discoveries including the identification of a specific i-motif antibody are pushing this field forward. This perspective reviews initial and current work characterizing the i-motif and providing insight into the biological function of this DNA structure, with a focus on how the i-motif can serve as a molecular target for developing new therapeutic approaches to modulate gene expression and extension of repetitive DNA.

scholarly journals Decoding the Epitranscriptional Landscape from Native RNA Sequences

2018 ◽  
Author(s):  
Thidathip Wongsurawat ◽  
Piroon Jenjaroenpun ◽  
Trudy M. Wassenaar ◽  
Taylor D Wadley ◽  
Visanu Wanchai ◽  
...  
Keyword(s):  
Growth Conditions ◽  
Untranslated Regions ◽  
Metabolic Adaptation ◽  
Rna Modifications ◽  
Rna Sequences ◽  
Rna Methylation ◽  
E Coli ◽  
Oxford Nanopore ◽  
G Quadruplex ◽  
Bioinformatic Tool

AbstractSequencing of native RNA and corresponding cDNA was performed using Oxford Nanopore Technology. The % Error of Specific Bases (%ESB) was higher for native RNA than for cDNA, which enabled detection of ribonucleotide modification sites. Based on %ESB differences of the two templates, a bioinformatic tool ELIGOS was developed and applied to rRNAs of E. coli, yeast and human cells. ELIGOS captured 91%, 95%, ∼75%, respectively, of the known variety of RNA methylation sites in these rRNAs. Yeast transcriptomes from different growth conditions were also compared, which identified an association between metabolic adaptation and inferred RNA modifications. ELIGOS was further applied to human transcriptome datasets, which identified the well-known DRACH motif containing N6-methyadenine being located close to 3’-untranslated regions of mRNA. Moreover, the RNA G-quadruplex motif was uncovered by ELIGOS. In summary, we have developed an experimental method coupled with bioinformatic software to uncover native RNA modifications and secondary-structures within transcripts.

scholarly journals G-Quadruplex-Based Drug Delivery Systems for Cancer Therapy

2021 ◽  
Vol 14 (7) ◽  
pp. 671
Author(s):  
Jéssica Lopes-Nunes ◽  
Paula Oliveira ◽  
Carla Cruz
Keyword(s):  
Drug Delivery ◽  
Small Molecules ◽  
Drug Delivery Systems ◽  
Delivery Systems ◽  
Nano Particles ◽  
Future Research ◽  
High Affinity ◽  
Dna And Rna ◽  
G Quadruplex ◽  
Gold Nano Particles

G-quadruplexes (G4s) are a class of nucleic acids (DNA and RNA) with single-stranded G-rich sequences. Owing to the selectivity of some G4s, they are emerging as targeting agents to overtake side effects of several potential anticancer drugs, and delivery systems of small molecules to malignant cells, through their high affinity or complementarity to specific targets. Moreover, different systems are being used to improve their potential, such as gold nano-particles or liposomes. Thus, the present review provides relevant data about the different studies with G4s as drug delivery systems and the challenges that must be overcome in the future research.

A look at photodynamic inactivation as a tool for pests and vector-borne diseases control

2022 ◽  
Vol 19 (2) ◽  
pp. 025601
Author(s):  
Alessandra R Lima ◽  
Lucas D Dias ◽  
Matheus Garbuio ◽  
Natalia M Inada ◽  
Vanderlei S Bagnato
Keyword(s):  
Public Health ◽  
Low Cost ◽  
Reactive Oxidative Species ◽  
Photodynamic Inactivation ◽  
Health Issues ◽  
Vector Borne Diseases ◽  
Control Techniques ◽  
The Past ◽  
Vector Borne ◽  
Oxidative Species

Abstract The control of pests and vector-borne diseases (VDBs) are considered public health issues Worldwide. Among the control techniques and pesticides used so far, photodynamic inactivation (PDI) has been shown as an eco-friendly, low cost, and efficient approach to eliminate pests and VDBs. PDI is characterized using a photosensitizing molecule, light and molecular oxygen (O2) resulting in production of reactive oxidative species which can promote the oxidation of biomolecules on pests and vectors. Herein, we review the past 51 years (1970–2021) regarding the use of photo pesticides, reporting the most important parameters for the protocol applied, the results obtained, and limitations. Moreover, we described the mechanism of action of the PDI, main classes of photopesticides used so far as well as the cell death mechanism resulting from the photodynamic action.

Abstract 14853: Acute Inhibition of Mitochondrial Reactive Oxidative Species Improves Endothelial SK Channel Function Following Cardioplegic Hypoxia/Reperfusion and Diabetes

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Yi Song ◽  
Zhiqi Zhang ◽  
Hang Xing ◽  
Yixin He ◽  
guangbin shi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Coronary Arteries ◽  
Ischemia Reperfusion ◽  
Mouse Heart ◽  
Reactive Oxidative Species ◽  
Sk Channels ◽  
Sk Channel ◽  
Relaxation Responses ◽  
Oxidative Species ◽  
Coronary Relaxation

Introduction: Cardioplegic-ischemia/reperfusion (CP-I/R) and diabetes mellitus (DM) are correlated with coronary endothelial dysfunction and inactivated small conductance calcium-activated-potassium (SK) channels. Increased reactive oxidative species (ROS), such as mitochondrial ROS (mROS) may contribute to oxidative injury. Thus, we hypothesized that inhibition of mROS may protect coronary SK channels and endothelial function against CP-I/R-induced injury. Objective: A cardioplegic hypoxia and reoxygenation (CP-H/R) model consisting of coronary endothelial cells and small coronary arteries with or without DM were employed for examining whether MT could protect against coronary endothelial and SK channel dysfunction. Methods: Small coronary arteries (<150μm) and endothelial cells (MHECs) were dissected from the mouse heart with non-diabetes (ND) and DM (n=6/group). The microvessels or MHECs were subjected to hypoxia with cardioplegia and re-oxygenated. The microvessels or MHECs were treated with or without MT (10 -5 M) 5-minutes before and during CP-hypoxia. Microvascular vasodilation function was assessed in vitro by administration of vasoconstrictor, then ADP or NS309,respectively. K + currents of MHECs were measured by whole-cell patch clamp. The levels of endothelial mROS was measured by MitoSox TM . Results: CP-H/R significantly attenuated endothelial SK channels activity and the coronary relaxation responses to ADP and NS309 in the ND and DM groups. Treatment with MT enhanced coronary relaxation responses to ADP or NS309 ( p <0.05, Fig 1. A, B), and similar findings were seen in endothelial SK channel currents in both ND and DM MHECs ( p <0.05, Fig 1. C-F). In addition, treating MHECs with MT reduced CP-H/R-induced mROS in ND and DM groups. Conclusions: Administration of MT improves endothelial SK channels activity which may contribute to its enhancement of endothelium-dependent vasorelaxation following CP-H/R.

Near-infrared-excited upconversion photodynamic therapy of extensively drug-resistant Acinetobacter baumannii based on lanthanide nanoparticles

Nanoscale ◽  
2020 ◽  
Vol 12 (26) ◽  
pp. 13948-13957
Author(s):  
Wenzhen Liu ◽  
Yuxiang Zhang ◽  
Wenwu You ◽  
Jianqiang Su ◽  
Shaohua Yu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Acinetobacter Baumannii ◽  
Near Infrared ◽  
Drug Resistant ◽  
Extensively Drug Resistant ◽  
Novel Strategy

This work reports a novel strategy to combat extensively drug-resistant Acinetobacter baumannii via NIR-responsive UCNPs-based PDT.

scholarly journals G-Quadruplexes in the Archaea Domain

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1349
Author(s):  
Václav Brázda ◽  
Yu Luo ◽  
Martin Bartas ◽  
Patrik Kaura ◽  
Otilia Porubiaková ◽  
...  
Keyword(s):  
Random Distribution ◽  
Dna Structures ◽  
Cellular Processes ◽  
Dna And Rna ◽  
Non Coding Rna ◽  
Archaeal Species ◽  
G Quadruplex ◽  
Potential Part ◽  
Functional Relevance ◽  
Eukaryotic Genomes

The importance of unusual DNA structures in the regulation of basic cellular processes is an emerging field of research. Amongst local non-B DNA structures, G-quadruplexes (G4s) have gained in popularity during the last decade, and their presence and functional relevance at the DNA and RNA level has been demonstrated in a number of viral, bacterial, and eukaryotic genomes, including humans. Here, we performed the first systematic search of G4-forming sequences in all archaeal genomes available in the NCBI database. In this article, we investigate the presence and locations of G-quadruplex forming sequences using the G4Hunter algorithm. G-quadruplex-prone sequences were identified in all archaeal species, with highly significant differences in frequency, from 0.037 to 15.31 potential quadruplex sequences per kb. While G4 forming sequences were extremely abundant in Hadesarchaea archeon (strikingly, more than 50% of the Hadesarchaea archaeon isolate WYZ-LMO6 genome is a potential part of a G4-motif), they were very rare in the Parvarchaeota phylum. The presence of G-quadruplex forming sequences does not follow a random distribution with an over-representation in non-coding RNA, suggesting possible roles for ncRNA regulation. These data illustrate the unique and non-random localization of G-quadruplexes in Archaea.

Sign in / Sign up

Export Citation Format

Share Document