scholarly journals Membrane Surface Features of Blastocystis Subtypes

Genes ◽  
2018 ◽  
Vol 9 (8) ◽  
pp. 417 ◽  
Author(s):  
John Yason ◽  
Kevin Tan

Blastocystis is a common intestinal protistan parasite with global distribution. Blastocystis is a species complex composed of several isolates with biological and morphological differences. The surface coats of Blastocystis from three different isolates representing three subtypes were analyzed using scanning electron microscopy. This structure contains carbohydrate components that are also present in surface glycoconjugates in other parasitic protozoa. Electron micrographs show variations in the surface coats from the three Blastocystis isolates. These differences could be associated with the differences in the pathogenic potential of Blastocystis subtypes. Apart from the surface coat, a plasma membrane-associated surface antigen has been described for Blastocystis ST7 and is associated with programmed cell death features of the parasite.

Author(s):  
Yan Yan ◽  
Benny K.K. Chan

Larvae of Chthamalus malayensis (Cirripedia: Thoracica) from Hong Kong were cultured in the laboratory. Larval development includes six naupliar stages and a non-feeding cypris stage. Larvae reached the cypris stage in 20 d at ∼21°C compared to 14 d at ∼28°C. Morphological features including the cephalic shield, frontal horns, labrum, abdominal process, antennules, antennae and mandibles in all nauplius and cypris stages were described and illustrated using a combination of light microscopy and scanning electron microscopy. Attempts were made to compare morphological differences between the nauplii and cyprid of C. malayensis with those of other Chthamalus species including C. stellatus, C. montagui, C. dentatus, C. fragilis, C. dalli, C. antennatus, C. fissus and C. challengeri. The present description of the nauplii of C. malayensis is not in agreement with the previous description of this species.


2015 ◽  
Vol 638 ◽  
pp. 27-30 ◽  
Author(s):  
Stefan Ioan Voicu ◽  
Alexandru Muhulet ◽  
Iulian Antoniac ◽  
Marijana Simina Corobea

This paper presents a comparative study of cellulose acetate membranes, respectively nitrocellulose membranes, synthesized under the same conditions for the retention of proteins from aqueous solutions. It has also been studied the hydrodynamic behavior of the membranes, measuring water, respectively ethanol flow rates, and the retention of proteins. The membranes have been characterized by scanning electron microscopy in order to study the morphological differences.


Zootaxa ◽  
2007 ◽  
Vol 1508 (1) ◽  
pp. 49-59 ◽  
Author(s):  
FERRAN PALERO ◽  
PERE ABELLÓ

The external morphology of the first phyllosoma larva of the spiny lobster Palinurus mauritanicus is described based on hatched larvae, and compared with those described in other Palinurids. This constitutes the first scanning electron microscopy description of a phyllosoma of the genus Palinurus and the first complete description of a phyllosoma stage of P. mauritanicus. The first phyllosoma larva of P. mauritanicus hatches in a very advanced stage, compared to other palinurid genera, which would allow it to stay in the plankton for a shorter period of time. We cannot point out any major morphological differences from the congeneric species Palinurus elephas, co-occurring in most of its distribution area. The first phyllosoma larva of P. mauritanicus can be differentiated from those described for the genus Panulirus as well as from those of Scyllarus and Scyllarides widely co-occurring in the distribution area of P. mauritanicus, by the pereiopods 4 and 5 being already present as small buds and by the long, non-setose exopod on pereiopod 3. The morphology and differential position of the chromatophores of the living animal are also described.


2020 ◽  
Vol 94 ◽  
Author(s):  
J.N. Caira ◽  
K. Jensen ◽  
C. Hayes ◽  
T.R. Ruhnke

Abstract Three new cestode species are described from the crocodile shark (Pseudocarcharias kamoharai) in Ecuador. All three were examined with light and scanning electron microscopy. The unique combination of morphological features in one of the new species prompted formal investigation of the non-monophyly of Paraorygmatobothrium relative to the morphologically similar genera Doliobothrium, Guidus, Marsupiobothrium, Nandocestus, Orectolobicestus, Ruhnkecestus and Scyphophyllidium. Sequence data generated for part of the 28S rDNA gene were subjected to maximum likelihood (ML) analysis. The resulting tree led to the synonymization of six of these seven genera with Scyphophyllidium, and transfer of their species to the latter genus. With the new species, the number of described members of Scyphophyllidium is now 45. The diagnosis of Scyphophyllidium is revised to accommodate these species. In addition, to expedite future descriptions, eight categories of Scyphophyllidium species are circumscribed, based largely on bothridial features. Scyphophyllidium timvickiorum n. sp. is a category 1 species. Beyond being the smallest category 1 species, it bears, rather than lacks, apical suckers and lacks, rather than bears, strobilar scutes. The two other new species are members of Clistobothrium. Clistobothrium amyae n. sp. differs from its congeners in bothridial shape, elongate cephalic peduncle and tiny size. Clistobothrium gabywalterorum n. sp. differs from the two of its congeners that also possess foliose bothridia in overall size and testis number. Despite their substantial morphological differences, the ML tree indicates they are sister taxa. Both are unique among their congeners in possessing cephalic peduncle spinitriches. The diagnosis of Clistobothrium is revised accordingly.


Zootaxa ◽  
2017 ◽  
Vol 4258 (5) ◽  
pp. 477 ◽  
Author(s):  
ANA CELIA MONTES DE OCA-AGUILAR ◽  
OSCAR MIKERY-PACHECO ◽  
ALFREDO CASTILLO ◽  
EDUARDO A. REBOLLAR-TÉLLEZ ◽  
PETER M. PIERMARINI ◽  
...  

The sand fly Lutzomyia cruciata has been associated with the transmission of Leishmania mexicana to humans in Mexico. This species has a wide distribution in Mexico occupying different microhabitats and environments. In this work comparisons of the egg exochorion of Lu. cruciata from different physiographic areas are presented. Study sites are from different states of southern Mexico. Exochorion analysis was carried out using scanning electron microscopy (SEM). Results show differences in the exochorionic pattern among samples from Veracruz (AVER), Yucatán (HOYU) and Chiapas (TACH). The morphotype “Chiapas” has a polygonal crest pattern, the morphotype “Veracruz” shows parallel and longitudinal crests with some or few connections, and the morphotype “Yucatán” has weak connections between crest ridges. These morphological differences could be the result of local adaptations or evidence of divergence within the nominal unit Lutzomyia cruciata.


Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1538
Author(s):  
Naji Kharouf ◽  
Ammar Eid ◽  
Louis Hardan ◽  
Rim Bourgi ◽  
Youri Arntz ◽  
...  

This study investigated the antibacterial activity, bond strength to dentin (SBS), and ultra-morphology of the polymer–dentin interface of experimental adhesive systems doped with pyrogallol (PY), which is a ubiquitous phenolic moiety that is present in flavonoids and polyphenols. A universal adhesive containing 4-META and 10-MDP was used in this study. PY behaves as an antioxidant and anti-cancerogenic agent and it was incorporated into the adhesive at different concentrations (0.5 and 1 wt.%). The antibacterial activity and SBS were analyzed and the results were statistically analyzed. The ultra-morphology of the polymer–dentin interface was assessed using scanning electron microscopy (SEM). At 24 h, a lower antibacterial activity was observed for the control adhesive compared to those with 0.5% and 1% PY. No difference was seen in SBS between the three groups at 24 h. After 6 months, the SBS of the 0.5% PY adhesive was significantly lower than the other tested adhesives. The specimens created with 1% PY adhesive presented a higher bond strength at six months compared with that found at 24 h. No morphological differences were found at the polymer–dentin interfaces of the tested adhesives. Pyrogallol may be incorporated into modern universal adhesive systems to preserve the polymer–dentin bonding interface and confer a certain degree of antibacterial activity.


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