scholarly journals Protein Ingredient in Saliva on Oral Dryness Patients Caused by Calcium Blocker

Geriatrics ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 70
Author(s):  
Fumi Mizuhashi ◽  
Takao Morita ◽  
Shuji Toya ◽  
Ritsuko Sato ◽  
Yuko Watarai ◽  
...  
Keyword(s):  
Flow Rate ◽  
Proteomic Analysis ◽  
Protein Concentration ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Type I ◽  
Protein Ingredient ◽  
Healthy Elderly ◽  
Calcium Blocker ◽  
Oral Dryness

Oral dryness as a side effect of certain drugs is increasing. The aim of this study was to examine the change of the protein ingredient in saliva of oral dryness patients caused by calcium blocker. Six patients taking calcium blocker and six healthy elderly were enrolled. Unstimulated salivary flow rate, protein concentration, and flow rate of protein were measured and compared between the patients taking calcium blocker and healthy elderly. iTRAQ (Isobaric Tag for Relative and Absolute Quantitation) proteomic analysis was performed to extract the salivary protein changed in patient taking calcium blocker, and the intensities of Western blotting products were quantified (unpaired t-test). Unstimulated salivary flow rate was significantly lower on patients taking calcium blocker (p < 0.01). Protein concentration tended to be higher and the flow rate of protein tended to be lower on patients. As the result of iTRAQ proteomic analysis, calmodulin-like protein 3, glutathione S-transferase P, and keratin type I cytoskeletal 13 increased characteristically in patient taking calcium blocker, and the expression in calmodulin-like protein 3 was significantly larger (p < 0.01). The results of this study indicated that calmodulin-like protein 3 increased in patients taking calcium blocker and could be a salivary biomarker for oral dryness caused by calcium blocker.

Dental Caries and Salivary Alterations in Type I Diabetes

2011 ◽  
Vol 36 (2) ◽  
pp. 181-184 ◽  
Author(s):  
Kavita Rai ◽  
Amitha Hegde ◽  
Ananth Kamath ◽  
Suchetha Shetty
Keyword(s):  
Dental Caries ◽  
Flow Rate ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Control Group ◽  
Diabetic Patients ◽  
Type I ◽  
Healthy Children ◽  
Salivary Ph ◽  
Anti Oxidant

Insulin dependent diabetes mellitus is a severe disease that raises blood glucose levels because of hyperglycemia and insulinopenia. Fluctuations in water and electrolyte levels may result in xerostomia and other changes in the salivary composition. Since diabetes has an influence on oral health, it is important for the dentist to be aware of newer advances in the field of diabetes and to recognize specific oral problems related to diabetes. Thus, the dentist becomes an important part of the health care team for the patients with diabetes. Aim: The present study correlated salivary flow rate, salivary pH and total salivary antioxidant levels and dental caries in type I diabetic patients. Method: A total of 200 children that included 100 known diabetic children (study group) and 100 healthy children (controls) of both the sexes and from similar socioeconomic backgrounds formed the part of this study. Dental caries was assessed using DMFT index. The salivary total anti-oxidant level was estimated using phospho molybdic acid using spectrophotometric method. The salivary flow rate was recorded using the Zunt method and the salivary pH using the pH indicating paper. The results were statistically analyzed using t-test. Conclusions: The analyzed parameters showed increase in salivary anti-oxidant levels, reduced salivary flow rate, increase incidence of dental caries, salivary pH was decreased when compared to the control group.

scholarly journals Correlation Between Clinical Oral Dryness Score and Salivary Flow Rates in Active and Passive Smokers

2021 ◽  
Vol 10 (02) ◽  
pp. 120-123
Author(s):  
Seeme Nigar ◽  
Sobia Hassan ◽  
Sidra Fahim ◽  
Ahmed Bin Khalid ◽  
Faisal Salim
Keyword(s):  
Flow Rate ◽  
Smoking Status ◽  
Saliva Sample ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Significant Difference ◽  
Oral Dryness ◽  
The Mean ◽  
Potential Factors ◽  
Oral Dryness Feeling

Objective: To evaluate the mean clinical oral dryness score (CODs) and salivary flow rate (SFR) in subjects exposed to active and passive smoking. The secondary aim was to determine the correlation between CODs and SFR and potential factors associated with CODs and SFR in our subjects. Study Design and Setting: The clinical observational study was conducted at the Outpatient department of oral diagnosis at Altamash Institute of Dental Medicine, Karachi from Jan 2019-Jul 2019. Methodology: A total of 217 participants of ages 15 – 80years coming for routine dental checkup of either gender were included. A thorough oral examination was performed for all the subjects. A non-stimulated saliva sample was collected from the oral cavity of subjects in a graduated container. The salivary flow rate was noted in ml/min for 5 minutes. Assessment of oral dryness/hypo-salivation was examined through CODs (clinical oral dryness score).The data were analyzed using SPSS software version 23. Results: The mean COD score and SFR were calculated as 1 and 0.42 ml/5min respectively. The Pearson’s correlation between COD score and SFR level was calculated as -0.281 (negative correlation) such as the SFR significantly decreased when the COD score increased (p<0.05). The age, gender, smoking status, betel quid and areca nut consumption showed statistically significant difference in mean COD score and SFR levels (p<0.05). Conclusion: Salivary flow rate was significantly decreased with increase in COD score thus having an implication on oral dryness feeling in these participants.

scholarly journals SALIVARY PROFILE AND IDENTIFICATION OF EXISTING CANDIDA sp OF AN INSTITUTIONALIZED ELDERLY POPULATION IN JAKARTA

2013 ◽  
Vol 17 (4) ◽  
pp. 334-338
Author(s):  
Febrina Rahmayanti ◽  
Yuniardini Septorini Wimardhani ◽  
Indriasti Wardhany
Keyword(s):  
Flow Rate ◽  
Elderly Population ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Normal Range ◽  
Institutionalized Elderly ◽  
Candida Sp ◽  
Salivary Ph ◽  
Oral Dryness ◽  
Oral Symptom

Saliva plays an important role to protect oral and dental health, in maintenance oral microflora balance. In elderly, disease, and medications usually influence the saliva. The purpose of this preliminary study was to investigate salivary profile, oral symptoms of dryness, and existing of Candida sp of an institutionalized elderly in Jakarta, Twenty seven (27) elderly (17 men and 10 women) were examined in a cross sectional study. Unstimulated whole saliva was collected for 5 minutes, according to published protocol, and then salivary flow rate and pH were measured. Candida sp were cultured in CHROM agar. The yeasts were counted and identified. Oral symptom or complaints of oral dryness, and burning or itching were recorded with an interview. Data of systemic conditions and medication the used were obtained from the medical record. Eighteen (66.7%) CHROM agar plates were Candida sp positive cultured, and about 61.1% subjects’ salivary pH were below normal range. Twenty one (77.8%) subjects had oral symptom and complain about oral dryness, even thought the salivary flow rate were in normal range. In this study, C.albicans is not the most frequently found colonized. About 72% were identified not C.albicans. This study suggests that, in elderly which salivary pH was below normal range have greater opportunistic infected by Candida sp. It can be concluded that, the result did not confirm a significant difference between subjects’ data and salivary profile concentration. The mycological finding Candida albicans is not the most frequently found colonized in this elderly population. Further study which lagger elderly population and might give deeper insights of salivary profile in this population.

scholarly journals Reliability of Screening Methods to Diagnose Oral Dryness and Evaluate Saliva Secretion

2020 ◽  
Vol 8 (3) ◽  
pp. 102
Author(s):  
Takaharu Goto ◽  
Takahiro Kishimoto ◽  
Yuki Iwawaki ◽  
Keiko Fujimoto ◽  
Yuichi Ishida ◽  
...  
Keyword(s):  
Flow Rate ◽  
Correlation Coefficients ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Screening Methods ◽  
Systematic Bias ◽  
Saliva Secretion ◽  
Whole Saliva ◽  
Oral Dryness ◽  
Unstimulated Whole Saliva

In this study, we evaluated the reliability and reproducibility of widely implemented salivary flow rate and oral dryness tests. In experiment 1, twenty young and healthy Japanese participants volunteered to participate. For each participant, the oral moisture (OM) level, unstimulated whole saliva volume (U-WSV), and stimulated whole saliva volume (S-WSV) were measured at the same time on two separate days. In experiment 2, twenty-seven patients who were over 65 years of age volunteered to participate. The OM level and U-WSV were measured at the same time on two separate days. In Experiment 1, the intra-class correlation coefficients (ICCs) corresponding to the S-WSV, U-WSV, and OM level were 0.23, 0.28, and 0.16, respectively, for the young participants. In Experiment 2, the ICCs corresponding to the U-WSV/spitting and OM level were 0.83 and 0.12, respectively, for the older participants. The results of Bland–Altman analysis confirmed the absence of systematic error, with the exception of the OM level results in Experiment 2, which indicated systematic bias. In conclusion, we believe that there is currently no consistent and reliable screening test for assessing salivary flow rate and oral dryness, although the spitting test was determined to be highly reliable.

scholarly journals Whey Protein Derived Mouthdrying Found to Relate Directly to Retention Post Consumption but Not to Induced Differences in Salivary Flow Rate

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 587
Author(s):  
Victoria Norton ◽  
Stella Lignou ◽  
Lisa Methven
Keyword(s):  
Whey Protein ◽  
Flow Rate ◽  
Protein Concentration ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Sensory Profile ◽  
Sensory Panel ◽  
Saliva Flow ◽  
Whey Beverages ◽  
Future Work

Whey protein is fortified into beverages to provide functional benefits, however, these beverages are considered mouthdrying. To date whey protein derived mouthdrying has not been quantified using a ‘physical measure’ in parallel with rated perception. Saliva flow could also relate to whey protein derived mouthdrying, however this has not been previously tested as an intervention. Accordingly, volunteers (n = 40) tested mouthdrying in different whey beverages and the sensory profile was evaluated by a trained sensory panel (n = 10). Volunteers also rated mouthdrying combined with collection of saliva samples post beverage consumption to measure retention to the oral cavity. To modulate saliva flow rate, volunteers both chewed on parafilm (to increase saliva flow) and used cotton wool (to remove saliva) before tasting beverages and rating mouthdrying. Both the volunteers and sensory panel rated whey protein beverages (WPB) as significantly more mouthdrying than the control beverage (whey permeate). The significantly higher rating of mouthdrying from the volunteers coincided with significantly higher protein concentration in saliva samples post WPB consumption, supporting mucoadhesion as the mechanism. Modulating saliva flow did not lead to any difference in rated mouthdrying and future work would be beneficial to evaluate further the influence of natural variation in salivary flow rate.

Psychological Stress and Its Influence on Salivary Flow Rate, Total Protein Concentration and IgA, IgG and IgM Titers

2010 ◽  
Vol 17 (6) ◽  
pp. 396-404 ◽  
Author(s):  
Nathália Matos-Gomes ◽  
Marilise Katsurayama ◽  
Fabiano Hiromichi Makimoto ◽  
Linda Luciana Oliveira Santana ◽  
Edijane Paredes-Garcia ◽  
...  
Keyword(s):  
Psychological Stress ◽  
Flow Rate ◽  
Total Protein ◽  
Protein Concentration ◽  
Salivary Flow ◽  
Salivary Flow Rate ◽  
Total Protein Concentration
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