Searching for the Role of the IFNγ rs2430561 Polymorphism in Inducible Inflammation: Contribution to Metabolic Syndrome in 45 to 60-Year-Old Women

Metabolic syndrome (MetS) is a cluster of conditions, increasing the risk of developing diseases that can lead to premature death. Interferon γ-inducible (the production of which is dependent on the IFNγ rs2430561 polymorphism) tryptophan-kynurenine inflammatory cascade helps to understand the increased association between inflammatory process and MetS, which is why we seek the relationship between the IFNγ gene polymorphisms and serum levels of markers of interferon-gamma (IFNγ)-inducible inflammatory cascade. The study sample consisted of 416 women, including 118 (28.4%) with MetS. The research procedure involved interview, anthropometric measurements, and blood collection. Kynurenine levels were significantly higher in the group of women with MetS. In the group with MetS, the A/T genotype of the IFNγ gene was accompanied by higher kynurenine levels. A direct relationship between the IFNγ gene polymorphisms and the rest of the markers of IFNγ-inducible inflammatory cascade was not confirmed with regard to MetS in 45 to 60-year-old women. A disparity in the kynurenine level, as well as the relationship between the presence of the A/T genotype of the IFNγ gene and a higher level of kynurenine in the group of women with MetS, may indicate an association between inflammation, metabolic disorders and tryptophan-kynurenine inflammatory cascade.

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Pathogenetic significance of C774T single nucleotide polymorphism of the endothelial NO synthase gene in the development of metabolic syndrome

Biomeditsinskaya Khimiya ◽

10.18097/pbmc20166204447 ◽

2016 ◽

Vol 62 (4) ◽

pp. 447-452 ◽

Cited By ~ 1

Author(s):

N.S. Fattakhov ◽

M.A. Vasilenko ◽

D.A. Skuratovskaia ◽

D.I. Kulikov ◽

E.V. Kirienkova ◽

...

Keyword(s):

Nitric Oxide ◽

Metabolic Syndrome ◽

Single Nucleotide Polymorphism ◽

Serum Levels ◽

Nucleotide Polymorphism ◽

Single Nucleotide ◽

Nos3 Gene ◽

Kaliningrad Region ◽

The Relationship

The relationship between nitric oxide production and metabolic disorders and the role of endothelial nitric oxide synthase (eNOS or NOS3) in metabolic syndrome (MS) remain poorly understood and need deeper investigation. In this context the role of the NOS3 gene in pathogenesis of MS is of special interest. The aim of the study was to investigate association of NOS3 single nucleotide polymorphism C774T with risk of MS in the Slavic population of the Kaliningrad region and the relationship of this polymorphic variant with some parameters of endothelial dysfunction. The study included 128 patients (48 men and 80 women aged from 36 to 52 years) with MS. The control group consisted of 126 healthy volunteers (60 men and 66 women aged from 30 to 40 years). Genotyping was performed by real-time PCR. Serum nitrite levels were determined spectrophotometrically by the Griess method. Serum levels of endothelin-1 and eNOS were evaluated by ELISA. The study has shown association of T allele (OR=2.06; p=0.0004; CI: 1.38-3.08) and CT genotype (OR=1.97; p=0.014; CI: 1.14-3.40 ) C774T polymorphism of the NOS3 gene with risk of MS in the Slavic population of the Kaliningrad region. Allele C (OR=0.48; p=0.0004; CI: 0.32-0.72) and homozygous CC genotype (OR=0.41; p=0.001; CI: 0.24-0,69) C774T polymorphism of the NOS3 gene were associated with reduced risk of the development of MS. Significant differences in serum levels of eNOS and endothelin-1 depended on the CT and TT genotypes of C774T polymorphism of the NOS3 gene in MS.

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Angiotensin-Converting Enzyme (ACE) D Allele as a Risk Factor for Increase Serum Interleukin-6 and Interleukin-8 in Psoriasis Patients

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2018.188 ◽

2018 ◽

Vol 6 (5) ◽

pp. 772-776 ◽

Cited By ~ 3

Author(s):

Ahmed I. Abd Elneam ◽

Mohammed Saleh Al-Dhubaibi ◽

Ali Ismaiel Ali Abd Alrheam

Keyword(s):

Risk Factor ◽

Healthy Subjects ◽

Serum Levels ◽

Converting Enzyme ◽

Inflammatory Skin Disease ◽

Ace Polymorphism ◽

Ace Gene ◽

Ace Gene Polymorphism ◽

The Relationship

BACKGROUND: Psoriasis is a chronic, recurrent inflammatory skin disease. It is characterised by autoimmune, environmental factors and complex genetic disorder.AIM: To explore the role of IL-6, IL-8, and ACE I/D polymorphism in the pathogenesis of Psoriasis and investigation of the relationship between ACE polymorphism and occurrence of psoriasis.PATIENTS AND METHODS: In this study, we took 73 psoriasis patients and 47 healthy patients as a control. These two groups subjected to analysis for ACE gene I/D polymorphism by PCR and biochemical methods.RESULTS: The serum levels of ACE, IL-8 and IL-6 were statistically significantly higher in psoriasis patients compared to healthy subjects (P < 0.001). ID and DD polymorphism were more common in psoriasis patients than healthy subjects. Also, D allele was significantly over-represented in patients compared to controls (52.7% Vs 35.1%).CONCLUSION: ACE gene polymorphism might grant susceptibility to develop psoriasis.

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Increased levels of circulating IL-10 in persons recovered from hepatitis C virus (HCV) infection compared with persons with active HCV infection

BMC Research Notes ◽

10.1186/s13104-020-05313-w ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Dorcas Ohui Owusu ◽

Richard Phillips ◽

Michael Owusu ◽

Fred Stephen Sarfo ◽

Margaret Frempong

Keyword(s):

Hepatitis C ◽

Spontaneous Recovery ◽

Predictive Marker ◽

Serum Levels ◽

Hcv Infection ◽

Tnf Alpha ◽

Hepatitis C Infection ◽

Ifn Gamma ◽

The Relationship

Abstract Objective Approximately 70% of all hepatitis C (HCV) infections develop chronic disease. Active or exacerbated chronic hepatitis C infection subsequently progress to liver disease. The role of T-cells secretions in achieving viral clearance is still not well understood. Thus, the current study was set to determine the relationship between the T cell cytokine profiles, biochemical parameters and persistent HCV infection or spontaneous recovery. Results Twenty-five percent (41/163) of the anti-HCV positive participants had recovered from HCV and had significantly higher concentration of IL-10 compared to those with active HCV infection (P < 0.012). Other circulating cytokines measured; IL-2, IFN gamma, TNF alpha, IL-5 and IL-17 were similar in both groups. Participants with active HCV infection had significantly higher aspartate transaminase (AST) (35 units) and alanine transaminase (46 units) compared to those in the recovered state (P < 0.001). Thus, serum levels of IL10 could be explored in larger prospective cohort study as a predictive marker of recovering from an active HCV infection.

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The relationship between tyramine levels and inflammation in metabolic syndrome

Hormone Molecular Biology and Clinical Investigation ◽

10.1515/hmbci-2019-0047 ◽

2019 ◽

Vol 40 (1) ◽

Cited By ~ 2

Author(s):

Ajay Patel ◽

Austin Thompson ◽

Lillian Abdelmalek ◽

Beverley Adams-Huet ◽

Ishwarlal Jialal

Keyword(s):

Metabolic Syndrome ◽

Early Morning ◽

National Institutes Of Health ◽

Atherosclerotic Cardiovascular Disease ◽

Multiple Biomarkers ◽

Early Biomarker ◽

Low Levels ◽

The Relationship

Abstract Background Metabolic syndrome (MetS) is an important contributor to both type 2 diabetes mellitus (T2DM) and atherosclerotic cardiovascular disease (ASCVD). Although MetS affects one third of American adults, its pathogenesis remains to be elucidated. Tyramine, a derivative of tyrosine, has been shown to act as a catecholamine releasing agent in the human body. The aim of this study is to investigate the role of tyramine as an early biomarker for nascent MetS without the confounding of T2DM, ASCVD or smoking. Patients and methods This was an exploratory study of 28 patients with nascent MetS and 20 matched controls carried out in 2018. Metabolites were evaluated from patient’s frozen early morning urine samples and were correlated with biomarkers of inflammation and adipokines. They were assayed by the National Institutes of Health (NIH) Western Metabolomics Center using liquid chromatography/mass spectrometry (LC/MS) and standardized to urinary creatinine. All patients had normal hepatic and renal function. Results Tyramine concentrations were significantly reduced in patients with MetS compared to controls, p = 0.0009. In addition, tyramine was significantly inversely correlated with multiple biomarkers of inflammation and cardiometabolic risk factors such as RBP4, monocyte TLR-4 abundance and P38MAPKinase activity, body mass index (BMI) and blood pressure (BP) (both systolic and diastolic). Conclusion In conclusion, low levels of tyramine could contribute to the proinflammatorty state of MetS.

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Role of interferon-γ gene polymorphisms in susceptibility to IgA nephropathy: a family-based association study

European Journal of Human Genetics ◽

10.1038/sj.ejhg.5201591 ◽

2006 ◽

Vol 14 (4) ◽

pp. 488-496 ◽

Cited By ~ 26

Author(s):

Francesco Paolo Schena ◽

◽

Giuseppina Cerullo ◽

Diletta Domenica Torres ◽

Francesco Scolari ◽

...

Keyword(s):

Association Study ◽

Iga Nephropathy ◽

Gene Polymorphisms ◽

Interferon Γ ◽

Family Based

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Association between Endothelial Nitric Oxide Synthase Polymorphisms and Risk of Metabolic Syndrome

Disease Markers ◽

10.1155/2013/584125 ◽

2013 ◽

Vol 34 (3) ◽

pp. 187-197 ◽

Cited By ~ 6

Author(s):

Chiu-Shong Liu ◽

Ru-Jiun Huang ◽

Fung-Chang Sung ◽

Cheng-Chieh Lin ◽

Chih-Ching Yeh

Keyword(s):

Metabolic Syndrome ◽

Endothelial Nitric Oxide Synthase ◽

Gene Polymorphisms ◽

Haplotype Analysis ◽

Variant Genotype ◽

Oxide Synthase ◽

Endothelial Nitric Oxide ◽

Self Administered Questionnaire ◽

Control Study

BACKGROUND: Previous studies inferring that theNOS3gene was associated with the pathogenesis of metabolic syndrome (MetS) had inconsistent findings. We investigated the role of threeNOS3polymorphisms (T-786C, intron 4b/a, and G894T) in the risk of MetS using a hospital-based case-control study.METHODS: We recruited 339 MetS cases and 783 non-MetS controls at a central Taiwanese hospital. Information on sociodemographic and lifestyle factors was obtained using a self-administered questionnaire. Genotypes ofNOS3polymorphisms were compared between cases and controls. Effects of interactions between gene polymorphisms and smoking and between gene polymorphisms and drinking on the risk of MetS were also determined.RESULTS: The T-786C TC+CC genotype was significantly associated with a decreased risk of MetS (odds ratio (OR), 0.63; 95% confidence interval (CI), 0.43–0.91), compared to the T-786C TT genotype, according to a logistic regression analysis. This beneficial effect was much greater for those who had ever smoked cigarettes (OR, 0.47; 95% CI, 0.26–0.87) or those who had not consumed alcohol (OR, 0.45; 95% CI, 0.26–0.77). In addition, the intron 4b/a variant genotype was marginally associated with a reduced risk of MetS (OR, 0.68; 95% CI, 0.47–1.00), compared to the intron 4b/a bb genotype, particularly for never alcohol consumers (OR, 0.56; 95% CI, 0.33–0.95). In the haplotype analysis, there was a 53% decrease in the MetS risk among C4bG haplotype carriers (OR, 0.47; 95% CI, 0.25–0.90), compared to those with the most common T4bG haplotype.CONCLUSIONS: Our results suggest that theNOS3T-786C and intron 4b/a polymorphisms may contribute to the risk of MetS. Further studies are needed to confirm the findings.

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Phloretin alleviates dinitrochlorobenzene-induced dermatitis in BALB/c mice

International Journal of Immunopathology and Pharmacology ◽

10.1177/2058738420929442 ◽

2020 ◽

Vol 34 ◽

pp. 205873842092944

Author(s):

Chieh-Shan Wu ◽

Shih-Chao Lin ◽

Shiming Li ◽

Yu-Chih Chiang ◽

Nicole Bracci ◽

...

Keyword(s):

Mouse Model ◽

Serum Levels ◽

Interferon Γ ◽

Chronic Inflammatory Disease ◽

Mitogen Activated Protein Kinase ◽

Normal Ranges ◽

Mitogen Activated Protein ◽

Ifn Γ ◽

Allergic Contact

Atopic dermatitis (AD) is a chronic inflammatory disease of the skin that substantially affects a patient’s quality of life. While steroids are the most common therapy used to temporally alleviate the symptoms of AD, effective and nontoxic alternatives are urgently needed. In this study, we utilized a natural, plant-derived phenolic compound, phloretin, to treat allergic contact dermatitis (ACD) on the dorsal skin of mice. In addition, the effectiveness of phloretin was evaluated using a mouse model of ACD triggered by 2,4-dinitrochlorobenzene (DNCB). In our experimental setting, phloretin was orally administered to BALB/c mice for 21 consecutive days, and then, the lesions were examined histologically. Our data revealed that phloretin reduced the process of epidermal thickening and decreased the infiltration of mast cells into the lesion regions, subsequently reducing the levels of histamine and the pro-inflammatory cytokines interleukin (IL)-6, IL-4, thymic stromal lymphopoietin (TSLP), interferon-γ (IFN-γ) and IL-17A in the serum. These changes were associated with lower serum levels after phloretin treatment. In addition, we observed that the mitogen-activated protein kinase (MAPK) and NF-κB pathways in the dermal tissues of the phloretin-treated rodents were suppressed compared to those in the AD-like skin regions. Furthermore, phloretin appeared to limit the overproliferation of splenocytes in response to DNCB stimulation, reducing the number of IFN-γ-, IL-4-, and IL-17A-producing CD4+ T cells in the spleen back to their normal ranges. Taken together, we discovered a new therapeutic role of phloretin using a mouse model of DNCB-induced ACD, as shown by the alleviated AD-like symptoms and the reversed immunopathological effects. Therefore, we believe that phloretin has the potential to be utilized as an alternative therapeutic agent for treating AD.

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The Relationship between Eicosanoid Levels and Serum Levels of Metabolic and Hormonal Parameters Depending on the Presence of Metabolic Syndrome in Patients with Benign Prostatic Hyperplasia

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16061006 ◽

2019 ◽

Vol 16 (6) ◽

pp. 1006

Author(s):

Katarzyna Grzesiak ◽

Aleksandra Rył ◽

Ewa Stachowska ◽

Marcin Słojewski ◽

Iwona Rotter ◽

...

Keyword(s):

Metabolic Syndrome ◽

Benign Prostatic Hyperplasia ◽

Free Testosterone ◽

Serum Levels ◽

Prostatic Hyperplasia ◽

Lipid Mediators ◽

Total Testosterone ◽

Blood Samples ◽

Mediators Of Inflammation ◽

The Relationship

Background: The purpose of our investigation was to analyze the relationship between the serum levels of inflammatory mediators (HETE, HODE) and the levels of selected metabolic and hormonal parameters in patients with benign prostatic hyperplasia (BPH) with regard to concomitant metabolic syndrome (MetS). Methods: The study involved 151 men with BPH. Blood samples were taken for laboratory analysis of the serum levels of metabolic and hormonal parameters. Gas chromatography was performed using an Agilent Technologies 7890A GC System. Results: We found that waist circumference was the only parameter related to the levels of fatty acids, namely: 13(S)-HODE, 9(S)-HODE, 15(S)-HETE, 12(S)-HETE, and 5-HETE. In the patients with BPH and MetS, triglycerides correlated with 9(S)-HODE, 15(S)-HETE, 12(S)-HETE, and 5-HETE, which was not observed in the patients without MetS. Similarly, total cholesterol correlated with 9(S)-HODE, and 15(S)-HETE in the patients with BPH and MetS, but not in those without MetS. In the group of BPH patients with MetS, total testosterone positively correlated with 13(S)-HODE, and free testosterone with 9(S)-HODE. Conclusions: Based on this study, it can be concluded that lipid mediators of inflammation can influence the levels of biochemical and hormonal parameters, depending on the presence of MetS in BPH patients.

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