scholarly journals Transfer of Macronutrients, Micronutrients, and Toxic Elements from Soil to Grapes to White Wines in Uncontaminated Vineyards

2021 ◽  
Vol 18 (24) ◽  
pp. 13271
Author(s):  
Justin B. Richardson ◽  
Jahziel K. Chase
Keyword(s):  
New York ◽  
Toxic Elements ◽  
Toxic Metal ◽  
Dilution Effect ◽  
Substantial Effect ◽  
Grape Berry ◽  
Human Consumption ◽  
White Wines ◽  
Grape Berries ◽  
Dietary Requirements

Wine is a popular beverage and may be a source of nutrient and toxic elements during human consumption. Here, we explored the variation in nutrient and toxic elements from soils to grape berries and commercial white wines (Chardonnay) at five USA vineyards (New York, Vermont, California, Virginia) with strongly contrasting geology, soils, and climates. Samples were analyzed for macronutrients (Ca, K, and Mg), micronutrients (Mn, Cu, and Zn), and toxic elements (As, Cd, and Pb). Our study showed contrasting macronutrient, micronutrient, and toxic element concentrations in soils and in vines, leaves, and grapes. However, plant tissue concentrations did not correspond with total soil concentrations, suggesting a disconnect governing their accumulation. Bioconcentration factors for soil to grape berry transfer suggest the accumulation of Ca, K and Mg in berries while Fe, Mn, Cu, Zn, and Pb were generally not accumulated in our study or in previous studies. Wines from the five vineyards studied had comparable nutrient, micronutrient, and toxic metal concentrations as wines from Germany, Italy, Portugal, Spain, Croatia, Czech Republic, and Japan. The transfer of nutrients and toxic elements from grape berries to wine indicated that only Ca, K, and Mg were added or retained while concentrations of all other micronutrients and toxic elements were somewhat to extensively diminished. Thus, there appears to be a substantial effect on the geochemistry of the wine from the grape from either the fermentation process (i.e., flocculation), or a dilution effect. We conclude that soils, geology, and climate do not appear to generate a unique geochemical terroir as the transfer and concentration of inorganic nutrients appear to be comparable across strongly contrasting vineyards. This has several implications for human health. Nutrients in wine have potential impacts for human nutrition, as wine can meet or exceed the recommended dietary requirements of Ca, K, Mg, and Fe, and toxic metals As and Pb concentrations were also non-trivial.

scholarly journals The impact of various grape stock cultivars on the As, Cu, Co and Zn content of the grape berry (must, seed)

2013 ◽  
pp. 39-44
Author(s):  
István Fekete ◽  
Nándor Rakonczás ◽  
Dávid Andrási ◽  
Éva Bódi ◽  
Szilárd Szilágyi ◽  
...  
Keyword(s):  
Toxic Elements ◽  
Grape Berry ◽  
Potentially Toxic Elements ◽  
Grape Berries ◽  
Zn Content ◽  
Abnormal Increase ◽  
Set Up ◽  
Arsenic Copper ◽  
The Impact ◽  
Cu And Zn

Scientific research from the last decades showed that the inappropriate industrial and agricultural production caused an abnormal increase of the potentially toxic elements in the soil. Unfortunately the acidification of the soil is an increasing problem in Hungary. According to Várallyay et al. (2008) 13% of the Hungarian soils are highly acid. Accumulation of toxic elements differs in the genetically diverse plant species. The root of the plant constitutes a filter so that the rootstock is also kind of a filter system, which may prevent that the scion part (such as berry) accumulate high levels of various potentially toxic elements from the soil. The aim of research was to determine how different grape rootstocks influence the As, Co, Cu and Zn content of the musts and seeds. Thus, specifying which of the grape rootstocks takes up the lowest level of these 4 elements (As, Co, Cu and Zn), and accumulates in berries, so could reduce the potentially toxic element load of the grape berries. The grape rootstock collection of the University of Debrecen was set up in 2003 in 3x1 m spacing on immune sandy soil. Grafting of ‘Cserszegi fűszeres’ was started in 2010. We could evaluate yields harvested from 12 rootstock varieties of the experiment in October 2011. We obtained valuable differences in the arsenic, copper, cobalt and zinc concentrations of musts and seeds of ‘Cserszegi fűszeres’ grafted into different rootstocks. The results obtained from the 2011 harvest support the statement that the choice of rootstock might be an important factor to increase food safety. The differences in concentration of the four elements observed in case of the rootstock may have been caused on one hand by the rootstock effect, and on the other hand, the vintage effect has a very significant impact on the vines element uptake. Several years of experimental results will be needed to answer these questions.

scholarly journals Characterization and Action Mechanism Analysis of VvmiR156b/c/d-VvSPL9 Module Responding to Multiple-Hormone Signals in the Modulation of Grape Berry Color Formation

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 896
Author(s):  
Ziwen Su ◽  
Xicheng Wang ◽  
Xuxian Xuan ◽  
Zilu Sheng ◽  
Haoran Jia ◽  
...  
Keyword(s):  
Color Change ◽  
Grape Berry ◽  
Anthocyanin Synthesis ◽  
Structural Genes ◽  
Expression Levels ◽  
Color Formation ◽  
Grape Berries ◽  
Ripening Stages ◽  
Color Conversion ◽  
Hormone Signals

In recent years, more and more reports have shown that the miR156-SPL module can participate in the regulation of anthocyanin synthesis in plants. However, little is known about how this module responds to hormonal signals manipulating this process in grapes. In this study, exogenous GA, ABA, MeJA, and NAA were used to treat the ‘Wink’ grape berries before color conversion, anthocyanin and other related quality physiological indexes (such as sugar, aroma) were determined, and spatio-temporal expression patterns of related genes were analyzed. The results showed that the expression levels of VvmiR156b/c/d showed a gradually rising trend with the ripening and color formation of grape berries, and the highest expression levels were detected at day 28 after treatment, while the expression level of VvSPL9 exhibited an opposite trend as a whole, which further verifies that VvmiR156b/c/d can negatively regulate VvSPL9. Besides, VvmiR156b/c/d was positively correlated with anthocyanin content and related genes levels, while the expression pattern of VvSPL9 showed a negative correlation. Analysis of promoter cis-elements and GUS staining showed that VvmiR156b/c/d contained a large number of hormone response cis-elements (ABA, GA, SA, MeJA, and NAA) and were involved in hormone regulation. Exogenous ABA and MeJA treatments significantly upregulated the expression levels of VvmiR156b/c/d and anthocyanin structural genes in the early stage of color conversion and made grape berries quickly colored. Interestingly, GA treatment downregulated the expression levels of VvmiR156b/c/d and anthocyanin structural genes in the early color-change period, but significantly upregulated in the middle color-change and ripening stages, therefore GA mainly modulated grape berry coloring in the middle- and late-ripening stages. Furthermore, NAA treatment downregulated the expression levels of VvmiR156b/c/d and anthocyanin structural genes and delayed the peak expression of genes. Meanwhile, to further recognize the potential functions of VvmiR156b/c/d, the mature tomato transient trangenetic system was utilized in this work. Results showed that transient overexpression of VvmiR156b/c/d in tomato promoted fruit coloring and overexpression of VvSPL9 inhibited fruit coloration. Finally, a regulatory network of the VvmiR156b/c/d-VvSPL9 module responsive to hormones modulating anthocyanin synthesis was developed. In conclusion, VvmiR156b/c/d-mediated VvSPL9 participated in the formation of grape color in response to multi-hormone signals.

scholarly journals Insights in genetic diversity of German and Italian grape berry moth (Eupoecilia ambiguella) populations using novel microsatellite markers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Annette Reineke ◽  
Alberto Pozzebon ◽  
Olivia Herczynski ◽  
Carlo Duso
Keyword(s):  
Population Genetics ◽  
Microsatellite Markers ◽  
Isolation By Distance ◽  
Resistance Management ◽  
Grape Berry ◽  
Grape Berries ◽  
Eupoecilia Ambiguella ◽  
Significant Damage ◽  
Significant Divergence ◽  
Lepidopteran Pest

AbstractThe grape berry moth Eupoecilia ambiguella (Lepidoptera: Tortricidae) is causing significant damage to grape berries, however, little is known on population genetics of this lepidopteran pest insect, hindered so far by the lack of suitable molecular markers. Here we report on the development of ten microsatellite markers of which six were used to characterise 21 E. ambiguella populations obtained from two viticultural regions in Germany and Italy. Moths were sampled during two subsequent generations (flights) in the same vineyard as well as in vineyards surrounded by different landscape types. German and Italian populations were genetically differentiated and a significant isolation by distance was evident. No significant divergence was observed among the populations from first or second flight moths, however, inbreeding was higher in first than in second flight populations. Moreover, inbreeding was influenced by habitat composition and complexity of landscape around vineyards, being positively associated with the percentage of area covered by grapevine. Population genetics of E. ambiguella could thus be affected by the presence of alternative host plants in viticultural landscapes, which is important in the light of both insecticide resistance management and sustainable pest management.

scholarly journals EFFECTS OF CALCIUM HYDROGEN PHOSPHATE ON LEAD UPTAKE BY COWPEA (VIGNA UNGUICULATA)

2020 ◽  
Vol 4 (3) ◽  
pp. 36-42
Author(s):  
M. S. Dagari ◽  
Mohammed Salisu Musa
Keyword(s):  
Vigna Unguiculata ◽  
Lead Nitrate ◽  
Atomic Absorption Spectrophotometry ◽  
Substantial Effect ◽  
Human Consumption ◽  
Hydrogen Phosphate ◽  
Lead Uptake ◽  
Cowpea Seeds ◽  
Phosphate Fertilization ◽  
Phosphate Treatment

The effects of calcium hydrogen phosphate on lead (Pb) uptake by cowpea (Vigna unguiculata) were investigated in a growth chamber experiment coupled with atomic absorption spectrophotometry (AAS) and colorimetry analyses. Cowpea seeds were planted and harvested in soil samples treated with Pb+2 added as lead nitrate at the rates of 0, 1000 and 3000 mg/kg followed by calcium hydrogen phosphate (CaHPO4) at 0, 10, 50 and 250 mg/kg. Increases in shoot yield brought about by phosphate fertilization were observed to oppress the shoot lead concentration as a result of growth dilution, where the actual amount of lead in shoots was unaffected by phosphate treatment. This work revealed that there is no substantial effect on lead uptake by cowpea, as a result of phosphate fertilization. Therefore, phosphate fertilization is a good means of cowpea production with minimal lead uptake and hence safe for human consumption

scholarly journals Increased Organic Fertilizer and Reduced Chemical Fertilizer Increased Fungal Diversity and the Abundance of Beneficial Fungi on the Grape Berry Surface in Arid Areas

2021 ◽  
Vol 12 ◽  
Author(s):  
Linnan Wu ◽  
Zhiqiang Li ◽  
Fengyun Zhao ◽  
Benzhou Zhao ◽  
Fesobi Olumide Phillip ◽  
...  
Keyword(s):  
Fungal Diversity ◽  
Organic Fertilizer ◽  
Grape Juice ◽  
Grape Berry ◽  
Chemical Fertilizer ◽  
Soluble Solids ◽  
Alpha And Beta Diversity ◽  
Grape Berries ◽  
Grape Quality ◽  
Beneficial Fungi

Fertilizer practices can significantly impact the fruit quality and microbial diversity of the orchards. The fungi on the surface of fruits are essential for fruit storability and safety. However, it is not clear whether fertilization affects the fungal diversity and community structure on the surface of grape berries. Here, grape quality and the fungal diversity on the surface of grapes harvested from three fertilizer treatments were analyzed shortly after grape picking (T0) and following 8 days of storage (T1). The study involved three treatments: (1) common chemical fertilizer for 2 years (CH); (2) increased organic fertilizer and reduced chemical fertilizer for 1 year (A.O); and (3) increased organic fertilizer and reduced chemical fertilizer for 2 years (B.O). The application of increased organic fertilizer and reduced chemical fertilizer increased the soluble solids content (SSC) of the grape berries and decreased the pH of the grape juice. A total of 827,947 high-quality fungal sequences were recovered and assigned to 527 operational taxonomic units. Members of the Ascomycota phylum were dominant in all samples and accounted for 94.41% of the total number of detected sequences, followed by the Basidiomycota (5.05%), and unidentified fungi (0.54%). Alpha and beta diversity analyses revealed significantly different fungal populations in the three fertilizer treatments over the two time periods. The fungal diversity and richness on the grape berry surface in the B.O and A.O treatments were higher than those in the CH treatment. Among the detected fungi, the B.O treatments were mainly Pichia, Aureobasidium, and Candida genera, while the CH treatments were Botrytis, Aspergillus, and Penicillium. Moreover, significant differences were revealed between the two assessment times (T0 and T1). The samples from the T0 timepoint had higher fungal richness and diversity than the samples from T1 timepoint. Increasing organic fertilizer usage in grape management could improve grape quality and went on to increase the fungal diversity, as well as the relative abundance (RA) of beneficial fungi on grape berry surfaces. The correlation analysis suggested that the pH of the grape juice was significantly negatively correlated with fungal diversity parameters.

scholarly journals Metrics for studying berry growth kinetics in seedless grape cultivars (Vitis vinifera L.)

OENO One ◽  
2021 ◽  
Vol 55 (1) ◽  
pp. 119-131
Author(s):  
George Letchov ◽  
Venelin Roychev ◽  
Neli Keranova
Keyword(s):  
Time Scale ◽  
Grape Berry ◽  
Rate Of Change ◽  
Lag Phase ◽  
Growth Phases ◽  
Growth Processes ◽  
Grape Berries ◽  
Growth Metrics ◽  
Seedless Grape ◽  
Sigmoid Curve

The process related to the changes in dimensions and mass of grape berry passes through two growth phases separated by a lag phase, and can be described by a double sigmoid curve. The onset of the growth phases and their duration are important factors for understanding the growth processes in grape berries. A new method for their quantitative determination was developed in the present study. In this method, the phase transition dates correspond to the times at which the rate of change of the curvature of the logistic (sigmoid) curve reaches an extreme value. The method was tested on three seedless grape varieties, Sultanina, Ruby Seedless and Rusalka 3, and the changes in grape berry dimensions and mass were tracked from anthesis to harvest. For each of the varieties, a double logistic model of change in berry length, width and mass from anthesis to harvest was developed and the metrics of growth - beginning, stabilisation and end of growth - for each of the two phases were determined. It was found that the metrics in mass and berry dimensions do not match and shift relative to each other over time. A comparison of growth metrics with phenological metrics, such as anthesis, veraison and ripening, showed that phenophases cannot be used as a time scale to record the acceleration of growth processes, as they shifted in time with growth metrics. An exception was veraison, which coincided with the beginning of the accelerated growth of grapes during the second growth phase, following the lag phase. The time scale presented in the current research is a new tool for monitoring growth processes and could help clarify the links between visible changes in the grape berries and the ongoing processes within them. The developed method can also be used for the analysis of various growth processes that follow the logistic law.

Cloning and expression of two plasma membrane aquaporins expressed during the ripening of grape berry

2003 ◽  
Vol 30 (6) ◽  
pp. 621 ◽  
Author(s):  
Sarah Picaud ◽  
Frédéric Becq ◽  
Fabienne Dédaldéchamp ◽  
Agnès Ageorges ◽  
Serge Delrot
Keyword(s):  
Plasma Membrane ◽  
Xenopus Oocytes ◽  
Potential Role ◽  
Grape Berry ◽  
Cloning And Expression ◽  
Vitis Vinifera L ◽  
Moderate Increase ◽  
Grape Berries ◽  
Urea Uptake

The ripening of grape (Vitis vinifera L.) berry is accompanied by dramatic accumulation of sugars and water. Two full-length clones and several partial clones encoding plasma membrane aquaporins (PIP) were cloned from grape berries collected at the beginning of ripening. Based on their sequences, on a phylogenetic analysis and on functional properties, both clones, called VvPIP1a and VvPIP1b were assigned to the PIP1 subfamily. RNA gel blot studies with berries at various stages of development indicated that VvPIP expression was highest at stages following veraison. Injection of Xenopus oocytes with VvPIP1a cRNA induced a moderate increase of water permeability and a large increase in glycerol permeability, whereas injection with VvPIP1b cRNA did not affect these permeabilities. Injection of VvPIP1a cRNA, but not VvPIP1b cRNA, inhibited urea uptake by the oocyte, and this inhibition was sensitive to HgCl2. The data are discussed in relation with the potential role of aquaporins in fruit physiology.

scholarly journals Microorganisms of Grape Berries

2017 ◽  
Vol 71 (6) ◽  
pp. 502-508 ◽  
Author(s):  
Attila Kántor ◽  
Ján Mareček ◽  
Eva Ivanišová ◽  
Margarita Terentjeva ◽  
Miroslava Kačániová
Keyword(s):  
Extraction Procedure ◽  
Health Condition ◽  
Grape Berry ◽  
Acid Extraction ◽  
Gram Negative Bacteria ◽  
Pesticide Application ◽  
Gram Positive ◽  
Gram Negative ◽  
Grape Berries ◽  
Gram Positive Bacteria

Abstract Grape surface is an unstable habitat that changes greatly according to the stage of grape ripening. Different bacteria and yeasts can colonise the surface of grape berry and the diversity of microorganisms depends on the stage of ripening, pesticide application and health condition. The aim of this study was to study the microflora of the surface of grape berries. Altogether, 19 grape samples from Slovakia were collected. The spread plate method was applied and a 100 μL inoculum of each dilution (10−2, 10−3) was plated on TSA, MEA, and MRS agar for isolation of microorganisms from grapes. Proteins were extracted from cells by ethanol/formic acid extraction procedure. MALDI-TOF Mass Spectrometry was used for identification of microorganisms. In total, 11 genera of Gram-negative bacteria, 11 of Gram-positive bacteria and nine of yeasts were identified. Among 200 isolates, Gram-negative, Gram-positive bacteria and yeasts represented 11%, 27% and 62% of the total number of isolates studied. The most common genera of isolated yeasts were Hanseniaspora (37%), Metschnikowia (31%), and Rhodotorula (10%). The most frequently isolated among Gram-negative bacteria were Acinetobacter (22%), Pseudomonas (22%) and Sphingomonas (13%). The most common genera of Gram-positive bacteria were Bacillus (20%), Lactobacillus (19%), Leuconostoc and Staphylococcus (11%), respectively.

The molecular regulation of stilbene phytoalexin biosynthesis in Vitis vinifera during grape berry development

2000 ◽  
Vol 27 (7) ◽  
pp. 723 ◽  
Author(s):  
Anthony J. Bais ◽  
Peter J. Murphy ◽  
Ian B. Dry
Keyword(s):  
Gene Expression ◽  
Vitis Vinifera ◽  
Stilbene Synthase ◽  
Grape Berry ◽  
Molecular Regulation ◽  
Vitis Vinifera L ◽  
Uv Treatment ◽  
Grape Berries ◽  
Uv Induction ◽  
Phytoalexin Biosynthesis

The molecular regulation of stilbene phytoalexin biosynthesis in developing Vitis vinifera L. grape berries was investigated using a UV induction system. Berries were collected at 1, 5, 10 and 16 weeks post-flowering from the cultivars Shiraz, Semillon, Cabernet Sauvignon and Chardonnay and the skins analysed for resveratrol production following irradiation with UV-C light. The rate and maximal level of resveratrol accumulation increased markedly in berries sampled from 1–5 weeks post-flowering and then dramatically declined in maturing berries sampled from 10–16 weeks post-flowering in all cultivars. In berries sampled at 1 and 5 weeks post-flowering, maximal levels of resveratrol accumulation were recorded at incubation periods of 24 and 48 h respectively whereas maximal resveratrol levels were not recorded in week 16 berry skins until 72 h after UV-treatment. Gene expression analysis indicated that stilbene synthase (STS) mRNA accumulated within 4–8 h of UV treatment in berries sampled at 1 and 5 weeks post-flowering, but did not increase in week 16 berries until 24–48 h following UV-irradiation. Furthermore, the overall level of STS gene expression declined in berries sampled 10–16 weeks post-flowering. The results demonstrate that inducible stilbene accumulation in ripening grape berries is highly regulated at the level of STS gene transcription. This decline in inducible STS gene expression may be a major factor contributing to the increased susceptibility of ripening grape berries to Botrytis cinerea infection.

Expression Patterns of Genes Encoding Sugar and Potassium Transport Proteins Are Simultaneously Upregulated or Downregulated When Carbon and Potassium Availability Is Modified in Shiraz (Vitis vinifera L.) Berries

2019 ◽  
Vol 60 (10) ◽  
pp. 2331-2342 ◽  
Author(s):  
Zelmari A Coetzee ◽  
Rob R Walker ◽  
Siyang Liao ◽  
Celia Barril ◽  
Alain J Deloire ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Phloem Transport ◽  
Potassium Transport ◽  
Grape Berry ◽  
Vitis Vinifera L ◽  
Grape Berries ◽  
Genes Encoding ◽  
Integral Role ◽  
The Relationship ◽  
And Storage

Abstract A link between the accumulation of sugar and potassium has previously been described for ripening grape berries. The functional basis of this link has, as of yet, not been elucidated but could potentially be associated with the integral role that potassium has in phloem transport. An experiment was conducted on Shiraz grapevines in a controlled environment. The accumulation of berry sugar was curtailed by reducing the leaf photoassimilation rate, and the availability of potassium was increased through soil fertilization. The study characterizes the relationship between the accumulation of sugar and potassium into the grape berry and describes how their accumulation patterns are related to the expression patterns of their transporter proteins. A strong connection was observed between the accumulation of sugar and potassium in the grape berry pericarp, irrespective of the treatment. The relative expression of proteins associated with sugar and potassium transport across the tonoplast and plasma membrane was closely correlated, suggesting transcriptional coregulation leading to the simultaneous translocation and storage of potassium and sugar in the grape berry cell.

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