scholarly journals TGF-β Activity Related to the use of Collagen Membranes: In Vitro Bioassays

2020 ◽  
Vol 21 (18) ◽  
pp. 6636 ◽  
Author(s):  
Layla Panahipour ◽  
Zahra Kargarpour ◽  
Bernadette Luza ◽  
Jung-Seok Lee ◽  
Reinhard Gruber
Keyword(s):  
Bone Regeneration ◽  
Nuclear Localization ◽  
Tissue Regeneration ◽  
Molecular Mechanisms ◽  
Transforming Growth Factor ◽  
Guided Bone Regeneration ◽  
Gingival Fibroblasts ◽  
Nadph Oxidase 4 ◽  
Collagen Membranes ◽  
Β Receptor

Collagen membranes commonly used in guided bone regeneration are supposed to actively influence tissue regeneration and are not exclusively serving as passive barriers shielding away the soft tissue. The molecular mechanisms by which collagen membranes might affect tissue regeneration might involve the activation of transforming growth factor beta (TGF-β) signaling pathways. Here, we determined the TGF-β activity of supernatants and proteolytic lysates of five commercially available collagen membranes. The expression of TGF-β target genes interleukin 11 (IL11), NADPH oxidase 4 (NOX4), and proteoglycan 4 (PRG4) was evaluated by reverse transcriptase polymerase chain reaction and IL11 immunoassay in gingival fibroblasts. TGF-β signaling activation was further assessed by blocking the TGF-β receptor I kinase, a TGF-β neutralizing antibody, and showing the nuclear localization of phosphorylated Smad3 and total Smad2/3. We could identify two collagen membranes whose supernatants and lysates caused a robust increase of TGF-β receptor I kinase-dependent expression of IL11 in gingival fibroblasts. Moreover, the supernatant of a particular one membrane caused the nuclear localization of phosphorylated Smad3 and Smad2/3 in the fibroblasts. These results strengthen the evidence that some collagen membranes possess an intrinsic TGF-β activity that might actively influence the process of guided bone regeneration.

scholarly journals Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jila Nasirzade ◽  
Zahra Kargarpour ◽  
Goran Mitulović ◽  
Franz Josef Strauss ◽  
Layla Panahipour ◽  
...  
Keyword(s):  
Cellular Response ◽  
Alveolar Bone ◽  
Quantitative Polymerase Chain Reaction ◽  
Genomic Analysis ◽  
Type I ◽  
Gingival Fibroblasts ◽  
Nadph Oxidase 4 ◽  
Major Response ◽  
Collagen Membranes ◽  
Β Receptor

AbstractParticulate autologous tooth roots are increasingly used for alveolar bone augmentation; however, the proteomic profile of acid dentin lysate and the respective cellular response have not been investigated. Here we show that TGF-β1 is among the 226 proteins of acid dentin lysate (ADL) prepared from porcine teeth. RNA sequencing identified 231 strongly regulated genes when gingival fibroblasts were exposed to ADL. Out of these genes, about one third required activation of the TGF-β receptor type I kinase including interleukin 11 (IL11) and NADPH oxidase 4 (NOX4). Reverse transcription-quantitative polymerase chain reaction and immunoassay confirmed the TGF-β-dependent expression of IL11 and NOX4. The activation of canonical TGF-β signaling by ADL was further confirmed by the phosphorylation of Smad3 and translocation of Smad2/3, using Western blot and immunofluorescence staining, respectively. Finally, we showed that TGF-β activity released from dentin by acid lysis adsorbs to titanium and collagen membranes. These findings suggest that dentin particles are a rich source of TGF-β causing a major response of gingival fibroblasts.

scholarly journals Selection of Collagen Membranes for Bone Regeneration: A Literature Review

Materials ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 786 ◽  
Author(s):  
Luca Sbricoli ◽  
Riccardo Guazzo ◽  
Marco Annunziata ◽  
Luca Gobbato ◽  
Eriberto Bressan ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Clinical Performance ◽  
Guided Bone Regeneration ◽  
Treatment Modalities ◽  
Barrier Membranes ◽  
Collagen Membranes ◽  
Collagen Versus ◽  
The Right ◽  
Grafting Materials ◽  
Selection Of

Several treatment modalities have been proposed to regenerate bone, including guided bone regeneration (GBR) where barrier membranes play an important role by isolating soft tissue and allowing bone to grow. Not all membranes biologically behave the same way, as they differ from their origin and structure, with reflections on their mechanical properties and on their clinical performance. Collagen membranes have been widely used in medicine and dentistry, because of their high biocompatibility and capability of promoting wound healing. Recently, collagen membranes have been applied in guided bone regeneration with comparable outcomes to non-resorbable membranes. Aim of this work is to provide a review on the main features, application, outcomes, and clinical employment of the different types of collagen membranes. Comparisons with non-resorbable membranes are clarified, characteristics of cross-linked collagen versus native collagen, use of different grafting materials and need for membrane fixation are explored in order to gain awareness of the indications and limits and to be able to choose the right membrane required by the clinical condition.

scholarly journals TGF-β receptor kinase inhibitor enhances growth and integrity of embryonic stem cell–derived endothelial cells

2003 ◽  
Vol 163 (6) ◽  
pp. 1303-1311 ◽  
Author(s):  
Tetsuro Watabe ◽  
Ayako Nishihara ◽  
Koichi Mishima ◽  
Jun Yamashita ◽  
Kiyoshi Shimizu ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Progenitor Cells ◽  
Molecular Mechanisms ◽  
Transforming Growth Factor ◽  
Kinase Inhibitor ◽  
Embryonic Stem ◽  
Vascular Growth ◽  
Vascular Progenitor Cells ◽  
Β Receptor ◽  
Synthetic Molecule

Recent findings have shown that embryonic vascular progenitor cells are capable of differentiating into mural and endothelial cells. However, the molecular mechanisms that regulate their differentiation, proliferation, and endothelial sheet formation remain to be elucidated. Here, we show that members of the transforming growth factor (TGF)-β superfamily play important roles during differentiation of vascular progenitor cells derived from mouse embryonic stem cells (ESCs) and from 8.5–days postcoitum embryos. TGF-β and activin inhibited proliferation and sheet formation of endothelial cells. Interestingly, SB-431542, a synthetic molecule that inhibits the kinases of receptors for TGF-β and activin, facilitated proliferation and sheet formation of ESC-derived endothelial cells. Moreover, SB-431542 up-regulated the expression of claudin-5, an endothelial specific component of tight junctions. These results suggest that endogenous TGF-β/activin signals play important roles in regulating vascular growth and permeability.

scholarly journals Bioresorbable Collagen Membranes for Guided Bone Regeneration

10.5772/34667 ◽  
2012 ◽  
Author(s):  
Haim Tal ◽  
Ofer Moses ◽  
Avital Kozlovsky ◽  
Carlos Nemcovsky
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Collagen Membranes

scholarly journals Evaluation of Bone Gain and Complication Rates after Guided Bone Regeneration with Titanium Foils: A Systematic Review

Materials ◽  
2020 ◽  
Vol 13 (23) ◽  
pp. 5346
Author(s):  
Elisabet Roca-Millan ◽  
Enric Jané-Salas ◽  
Albert Estrugo-Devesa ◽  
José López-López
Keyword(s):  
Systematic Review ◽  
Clinical Trials ◽  
Bone Regeneration ◽  
Randomized Clinical Trials ◽  
Guided Bone Regeneration ◽  
Cochrane Library ◽  
Complication Rates ◽  
Native Bone ◽  
Collagen Membranes ◽  
Bone Gain

Guided bone regeneration techniques are increasingly used to enable the subsequent placement of dental implants. This systematic review aims to analyze the success rate of these techniques in terms of bone gain and complications rate using titanium membranes as a barrier element. Electronic and hand searches were conducted in PubMed/Medline, Scielo, Scopus and Cochrane Library databases for case reports, case series, cohort studies and clinical trials in humans published up to and including 19 September 2020. Thirteen articles were included in the qualitative analysis. Bone gain both horizontally and vertically was comparable to that obtained with other types of membranes more commonly used. The postoperative complication rate was higher that of native collagen membranes and non-resorbable titanium-reinforced membranes, and similar that of crosslinked collagen membranes and titanium meshes. The survival rate of the implants was similar to that of implants placed in native bone. Due to the limited scientific literature published on this issue, more randomized clinical trials comparing occlusive titanium barriers and other types of membranes are necessary to reach more valid conclusions.

scholarly journals Evaluation of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Cross-Linked Collagen Membranes for Guided Bone Regeneration in Beagle Dogs

Materials ◽  
2020 ◽  
Vol 13 (20) ◽  
pp. 4599
Author(s):  
Jong-Ju Ahn ◽  
Hyung-Joon Kim ◽  
Eun-Bin Bae ◽  
Won-Tak Cho ◽  
YunJeong Choi ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Physical Properties ◽  
Bone Regeneration ◽  
Enzymatic Degradation ◽  
Test Group ◽  
Guided Bone Regeneration ◽  
Control Group ◽  
Collagen Membrane ◽  
Micro Computed Tomography ◽  
Collagen Membranes

The purpose of this study was to evaluate the bone regeneration efficacy of an 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked collagen membrane for guided bone regeneration (GBR). A non-cross-linked collagen membrane (Control group), and an EDC-cross-linked collagen membrane (Test group) were used in this study. In vitro, mechanical, and degradation testing and cell studies were performed. In the animal study, 36 artificial bone defects were formed in the mandibles of six beagles. Implants were inserted at the time of bone grafting, and membranes were assigned randomly. Eight weeks later, animals were sacrificed, micro-computed tomography was performed, and hematoxylin-eosin stained specimens were prepared. Physical properties (tensile strength and enzymatic degradation rate) were better in the Test group than in the Control group. No inflammation or membrane collapse was observed in either group, and bone volumes (%) in defects around implants were similar in the two groups (p > 0.05). The results of new bone areas (%) analysis also showed similar values in the two groups (p > 0.05). Therefore, it can be concluded that cross-linking the collagen membranes with EDC is the method of enhancing the physical properties (tensile strength and enzymatic degradation) of the collagen membranes without risk of toxicity.

scholarly journals Guided bone regeneration with subperiosteal implants of PTFE and hydroxyapatite physical barriers in rats

2003 ◽  
Vol 14 (2) ◽  
pp. 119-124 ◽  
Author(s):  
Nelson Luiz de Macedo ◽  
Luís Guilherme Scavone de Macedo ◽  
Fábio da Silva Matuda ◽  
Suzana Martins Ouchi ◽  
Adriana Socorro Ferreira Monteiro ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Bone Defects ◽  
Guided Bone Regeneration ◽  
Physical Barrier ◽  
Group Iii ◽  
Group I ◽  
Membrane Technique ◽  
Physical Barriers ◽  
Membrane Design

Regeneration of periodontal and alveolar ridge defects utilizing membranes is a well-established procedure in reconstructive surgery. Biomaterial characteristics and membrane design employed in guided tissue regeneration (GTR) techniques play an important role in good results. The purpose of this histologic experimental study in rats was to compare the use of two physical barriers in the osteopromotion by using GTR principles in bone defects created in tibias. Fifteen animals divided into 3groups were used: group I (non-porous polytetrafluoroethylene (PTFE) barrier), group II (coral hydroxyapatite (HA) blocks), and group III (defects that received no physical barrier). Histological examination showed varied amounts of newly formed bone beneath both types of barriers. The non-porous PTFE barrier showed better results than the HA group. The results of this study suggest that bone regeneration can be successfully enhanced by a submerged membrane technique.

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