scholarly journals Overexpression of a Poplar RING-H2 Zinc Finger, Ptxerico, Confers Enhanced Drought Tolerance via Reduced Water Loss and Ion Leakage in Populus

2020 ◽  
Vol 21 (24) ◽  
pp. 9454
Author(s):  
Min-Ha Kim ◽  
Jin-Seong Cho ◽  
Eung-Jun Park ◽  
Hyoshin Lee ◽  
Young-Im Choi ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Zinc Finger ◽  
Water Loss ◽  
Focal Point ◽  
Global Climate ◽  
Populus Trichocarpa ◽  
Ion Leakage ◽  
Woody Perennials ◽  
Transpirational Water Loss

Drought stress is one of the major environmental problems in the growth of crops and woody perennials, but it is getting worse due to the global climate crisis. XERICO, a RING (Really Interesting New Gene) zinc-finger E3 ubiquitin ligase, has been shown to be a positive regulator of drought tolerance in plants through the control of abscisic acid (ABA) homeostasis. We characterized a poplar (Populus trichocarpa) RING protein family and identified the closest homolog of XERICO called PtXERICO. Expression of PtXERICO is induced by both salt and drought stress, and by ABA treatment in poplars. Overexpression of PtXERICO in Arabidopsis confers salt and ABA hypersensitivity in young seedlings, and enhances drought tolerance by decreasing transpirational water loss. Consistently, transgenic hybrid poplars overexpressing PtXERICO demonstrate enhanced drought tolerance with reduced transpirational water loss and ion leakage. Subsequent upregulation of genes involved in the ABA homeostasis and drought response was confirmed in both transgenic Arabidopsis and poplars. Taken together, our results suggest that PtXERICO will serve as a focal point to improve drought tolerance of woody perennials.

Zinc-finger protein MdBBX7/MdCOL9, a target of MdMIEL1 E3 ligase, confers drought tolerance in apple

2021 ◽  
Author(s):  
Pengxiang Chen ◽  
Fang Zhi ◽  
Xuewei Li ◽  
Wenyun Shen ◽  
Mingjia Yan ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
E3 Ligase ◽  
26S Proteasome ◽  
Negative Regulator ◽  
Binding Motif ◽  
Ethylene Response Factor ◽  
Finger Protein

Abstract Water deficit is one of the main challenges for apple (Malus × domestica) growth and productivity. Breeding drought-tolerant cultivars depends on a thorough understanding of the drought responses of apple trees. Here, we identified the zinc-finger protein B-BOX 7/CONSTANS-LIKE 9 (MdBBX7/MdCOL9), which plays a positive role in apple drought tolerance. The overexpression of MdBBX7 enhanced drought tolerance, whereas knocking down MdBBX7 expression reduced it. Chromatin immunoprecipitation-sequencing (ChIP-seq) analysis identified one cis-element of MdBBX7, CCTTG, as well as its known binding motif, the T/G box. ChIP-seq and RNA-seq identified 1,197 direct targets of MdBBX7, including ETHYLENE RESPONSE FACTOR (ERF1), EARLY RESPONSIVE TO DEHYDRATION 15 (ERD15), and GOLDEN2-LIKE 1 (GLK1) and these were further verified by ChIP-qPCR and electronic mobility shift assays. Yeast two-hybrid screen identified an interacting protein of MdBBX7, RING-type E3 ligase MYB30-INTERACTING E3 LIGASE 1 (MIEL1). Further examination revealed that MdMIEL1 could mediate the ubiquitination and degradation of MdBBX7 by the 26S proteasome pathway. Genetic interaction analysis suggested that MdMIEL1 acts as an upstream factor of MdBBX7. In addition, MdMIEL1 was a negative regulator of the apple drought stress response. Taken together, our results illustrate the molecular mechanisms by which the MdMIEL1–MdBBX7 module influences the response of apple to drought stress.

scholarly journals The Barley Stripe Mosaic Virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

2019 ◽  
Author(s):  
Mario Houde ◽  
Arnaud Cheuk ◽  
François Ouellet
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Mosaic Virus ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Expression System ◽  
Functional Characterization ◽  
Barley Stripe Mosaic Virus ◽  
Finger Protein

Abstract Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization.Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected.Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

scholarly journals Variability in Water Loss Patterns of New Guinea Impatiens Cultivars and Breeding Selections

1995 ◽  
Vol 120 (3) ◽  
pp. 527-531
Author(s):  
Mark S. Strefeler ◽  
Robert-Jan W. Quené
Keyword(s):  
Drought Tolerance ◽  
Water Loss ◽  
New Guinea ◽  
Drought Tolerant ◽  
Commercial Cultivars ◽  
Transpirational Water Loss ◽  
Impatiens Hawkeri ◽  
New Guinea Impatiens ◽  
Breeding Selections ◽  
Selection Of

Six commercial cultivars (Anna, Aurore, Danhill, Danlight, Melanie, and Thelca), one drought tolerant cultivar (Orangeade), nine breeding selections, and one check genotype of Impatiens hawkeri Bull were evaluated for differences in drought tolerance based on water loss and time to wilt. The six commercially available cultivars had significantly higher mean water loss than the breeding selections and `Orangeade'. These cultivars wilted in 5.11 vs. 7.33 days for `Orangeade' and 9.10 days for the breeding selections. These results suggest that sufficient variability exists in New Guinea impatiens germplasm for the reduction of water loss to improve drought tolerance. Regression analysis revealed that total transpirational water loss 96 hours after withholding water was an excellent predictor of the time to wilting (a simple measure of drought tolerance) after water was withheld (R2 = 0.95). Thus, a simple, efficient and objective method for selection of drought tolerant genotypes has been developed for New Guinea impatiens. A comparison of offspring to parental genotypes showed that after only one cycle of selection, water loss was significantly reduced by >30%. These results suggest that there is sufficient genetic variability present for the development of more drought tolerant cultivars.

scholarly journals The Barley Stripe Mosaic Virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

2020 ◽  
Author(s):  
Arnaud Cheuk ◽  
François Ouellet ◽  
Mario Houde
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Mosaic Virus ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Expression System ◽  
Functional Characterization ◽  
Barley Stripe Mosaic Virus ◽  
Finger Protein

Abstract Background Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization. Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected. Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

Water loss prevention plays a greater role than ROS scavenging in dehydration tolerance of Kalanchoe tubiflora epiphyllous buds

2015 ◽  
Vol 62 (3) ◽  
pp. 153-159 ◽  
Author(s):  
Yin Ling Luo ◽  
Zhi Long Su ◽  
Xian Liang Cui ◽  
Qin Ying Lan
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Water Loss ◽  
Seedling Survival ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Ros Scavenging ◽  
Superoxide Anion Production ◽  
Kalanchoe Tubiflora ◽  
Epiphyllous Buds

Different plants and plant organs have various strategies to cope with drought stress.Kalanchoe tubifloraplants have a strong ability to prevent water loss and to scavenge reactive oxygen species. The epiphyllous bud of the plant serves as a reproduction unit and is able to generate plantlets even under conditions of extreme drought. The aim of this project was to study the antioxidant-stress response and osmoregulation ofK. tubifloraepiphyllous buds to dehydration and to compare the drought-tolerance mechanisms of the plant body and the epiphyllous buds. With the progression of bud dehydration, relative water content, seedling survival rate and dry weight per seedling decreased, and electrolyte leakage, hydrogen peroxide and malondialdehyde content and superoxide anion production rate increased. The activities of the antioxidant enzymes superoxide dismutase, catalase, ascorbate peroxidase, dehydroascorbate reductase and glutathione reductase decreased under dehydration. The content of proline, soluble sugars and soluble protein increased in dehydrated buds proportionally with the extent of water loss. These data indicate that the drought-tolerance mechanism of theK. tubiflorabud was different from that of its mother plant. The buds invest more energy to prevent water loss during drought stress, and their antioxidant defense weakens.

scholarly journals Present Status and Future Prospects of Drought Tolerance in Rice

2021 ◽  
Author(s):  
Veerendra Jaldhani ◽  
Ponnuvel Senguttuvel ◽  
Bathula Srikanth ◽  
Puskur Raghuveer Rao ◽  
Desiraju Subrahmanyam ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Global Climate ◽  
Growth And Yield ◽  
Rice Varieties ◽  
Drought Tolerant ◽  
Area Of Concern ◽  
Genomic Regions ◽  
Upland Conditions ◽  
Good Number

Rice is an important staple food crop across the world. It is mainly cultivated under irrigated lowland and also rain-fed upland conditions where drought stress is often noticed. Global climate change predicts an intensification of drought stress in future due to uneven rainfall which was witnessed for the last few years. Confronting drought stress can deliver fruitful crop returns in rice and scope for research extents. Drought stress affects the overall plant growth and yield. A prominent improvement has been made during last two decades in our understanding of the mechanisms involved in adaptation and tolerance to drought stress in rice. In order to achieve the marked crop returns from rainfed areas, there is a requisite of drought tolerant rice varieties, and genetic improvement for drought tolerance should be a prime area of concern in the future. A huge rice germplasm is available and good number of the germplasm possess drought tolerance and these genomic regions have been exploited in developing some drought tolerant rice varieties. The application of available genotyping methodologies, the identification of traits of interest, and key genetic regions associated with the drought tolerance have opened new prospects to successfully develop new drought tolerant varieties. This chapter deals with the importance of drought tolerance in rice crop followed by the evolution of molecular markers and breeding techniques in identifying drought tolerant QTL’s/genes and their utilization in the improvement of drought tolerant rice varieties.

scholarly journals Root and shoot variation in relation to potential intermittent drought adaptation of Mesoamerican wild common bean (Phaseolus vulgaris L.)

2018 ◽  
Vol 124 (6) ◽  
pp. 917-932 ◽  
Author(s):  
Jorge C Berny Mier y Teran ◽  
Enéas R Konzen ◽  
Viviana Medina ◽  
Antonia Palkovic ◽  
Andrea Ariani ◽  
...  
Keyword(s):  
Drought Stress ◽  
Phaseolus Vulgaris ◽  
Drought Tolerance ◽  
Common Bean ◽  
Global Climate ◽  
Crop Productivity ◽  
Climatic Conditions ◽  
Total Biomass ◽  
Drought Adaptation ◽  
Wild Beans

Abstract Background Wild crop relatives have been potentially subjected to stresses on an evolutionary time scale prior to domestication. Among these stresses, drought is one of the main factors limiting crop productivity and its impact is likely to increase under current scenarios of global climate change. We sought to determine to what extent wild common bean (Phaseolus vulgaris) exhibited adaptation to drought stress, whether this potential adaptation is dependent on the climatic conditions of the location of origin of individual populations, and to what extent domesticated common bean reflects potential drought adaptation. Methods An extensive and diverse set of wild beans from across Mesoamerica, along with a set of reference Mesoamerican domesticated cultivars, were evaluated for root and shoot traits related to drought adaptation. A water deficit experiment was conducted by growing each genotype in a long transparent tube in greenhouse conditions so that root growth, in addition to shoot growth, could be monitored. Results Phenotypic and landscape genomic analyses, based on single-nucleotide polymorphisms, suggested that beans originating from central and north-west Mexico and Oaxaca, in the driest parts of their distribution, produced more biomass and were deeper-rooted. Nevertheless, deeper rooting was correlated with less root biomass production relative to total biomass. Compared with wild types, domesticated types showed a stronger reduction and delay in growth and development in response to drought stress. Specific genomic regions were associated with root depth, biomass productivity and drought response, some of which showed signals of selection and were previously related to productivity and drought tolerance. Conclusions The drought tolerance of wild beans consists in its stronger ability, compared with domesticated types, to continue growth in spite of water-limited conditions. This study is the first to relate bean response to drought to environment of origin for a diverse selection of wild beans. It provides information that needs to be corroborated in crosses between wild and domesticated beans to make it applicable to breeding programmes.

scholarly journals The Barley Stripe Mosaic Virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

2020 ◽  
Author(s):  
Arnaud Cheuk ◽  
François Ouellet ◽  
Mario Houde
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Mosaic Virus ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Expression System ◽  
Functional Characterization ◽  
Barley Stripe Mosaic Virus ◽  
Finger Protein

Abstract Background Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization. Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected. Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

scholarly journals Ectopic expression of Medicago truncatula homeodomain finger protein, MtPHD6, enhances drought tolerance in Arabidopsis

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Wenli Quan ◽  
Xun Liu ◽  
Lihua Wang ◽  
Mingzhu Yin ◽  
Li Yang ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Drought Stress ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Zinc Finger ◽  
Medicago Truncatula ◽  
Ectopic Expression ◽  
Transcriptional Networks ◽  
Leaf Water Content ◽  
Antioxidant Enzyme Activities

Abstract Background The plant homeodomain (PHD) finger is a Cys4HisCys3-type zinc finger which promotes protein-protein interactions and binds to the cis-acting elements in the promoter regions of target genes. In Medicago truncatula, five PHD homologues with full-length sequence were identified. However, the detailed function of PHD genes was not fully addressed. Results In this study, we characterized the function of MtPHD6 during plant responses to drought stress. MtPHD6 was highly induced by drought stress. Ectopic expression of MtPHD6 in Arabidopsis enhanced tolerance to osmotic and drought stresses. MtPHD6 transgenic plants exhibited decreased water loss rate, MDA and ROS contents, and increased leaf water content and antioxidant enzyme activities under drought condition. Global transcriptomic analysis revealed that MtPHD6 reprogramed transcriptional networks in transgenic plants. Expression levels of ABA receptor PYR/PYLs, ZINC FINGER, AP2/EREBP and WRKY transcription factors were mainly up-regulated after transformation of MtPHD6. Interaction network analysis showed that ZINC FINGER, AP2/EREBP and WRKY interacted with each other and downstream stress induced proteins. Conclusions We proposed that ZINC FINGER, AP2/EREBP and WRKY transcription factors were activated through ABA dependent and independent pathways to increase drought tolerance of MtPHD6 transgenic plants.

scholarly journals iTRAQ-Based Proteomic Analysis Reveals Several Strategies to Cope with Drought Stress in Maize Seedlings

2019 ◽  
Vol 20 (23) ◽  
pp. 5956 ◽  
Author(s):  
Zhilei Jiang ◽  
Fengxue Jin ◽  
Xiaohui Shan ◽  
Yidan Li
Keyword(s):  
Protein Synthesis ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Membrane Trafficking ◽  
Water Loss ◽  
Drought Response ◽  
Osmotic Regulation ◽  
Maize Seedlings ◽  
Ros Scavenging ◽  
Drought Avoidance

Drought stress, especially during the seedling stage, seriously limits the growth of maize and reduces production in the northeast of China. To investigate the molecular mechanisms of drought response in maize seedlings, proteome changes were analyzed. Using an isotopic tagging relative quantitation (iTRAQ) based method, a total of 207 differentially accumulated protein species (DAPS) were identified under drought stress in maize seedlings. The DAPS were classified into ten essential groups and analyzed thoroughly, which involved in signaling, osmotic regulation, protein synthesis and turnover, reactive oxygen species (ROS) scavenging, membrane trafficking, transcription related, cell structure and cell cycle, fatty acid metabolism, carbohydrate and energy metabolism, as well as photosynthesis and photorespiration. The enhancements of ROS scavenging, osmotic regulation, protein turnover, membrane trafficking, and photosynthesis may play important roles in improving drought tolerance of maize seedlings. Besides, the inhibitions of some protein synthesis and slowdown of cell division could reduce the growth rate and avoid excessive water loss, which is possible to be the main reasons for enhancing drought avoidance of maize seedlings. The incongruence between protein and transcript levels was expectedly observed in the process of confirming iTRAQ data by quantitative real-time polymerase chain reaction (qRT-PCR) analysis, which further indicated that the multiplex post-transcriptional regulation and post-translational modification occurred in drought-stressed maize seedlings. Finally, a hypothetical strategy was proposed that maize seedlings coped with drought stress by improving drought tolerance (via. promoting osmotic adjustment and antioxidant capacity) and enhancing drought avoidance (via. reducing water loss). Our study provides valuable insight to mechanisms underlying drought response in maize seedlings.

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