Molecular and Biochemical Basis of Fluoroquinolones-Induced Phototoxicity—The Study of Antioxidant System in Human Melanocytes Exposed to UV-A Radiation

Phototoxicity of fluoroquinolones is connected with oxidative stress induction. Lomefloxacin (8-halogenated derivative) is considered the most phototoxic fluoroquinolone and moxifloxacin (8-methoxy derivative) the least. Melanin pigment may protect cells from oxidative damage. On the other hand, fluoroquinolone–melanin binding may lead to accumulation of drugs and increase their toxicity to skin. The study aimed to examine the antioxidant defense system status in normal melanocytes treated with lomefloxacin and moxifloxacin and exposed to UV-A radiation. The obtained results demonstrated that UV-A radiation enhanced only the lomefloxacin-induced cytotoxic effect in tested cells. It was found that fluoroquinolones alone and with UV-A radiation decreased superoxide dismutase (SOD) activity and SOD1 expression. UV-A radiation enhanced the impact of moxifloxacin on hydrogen peroxide-scavenging enzymes. In turn, lomefloxacin alone increased the activity and the expression of catalase (CAT) and glutathione peroxidase (GPx), whereas UV-A radiation significantly modified the effects of drugs on these enzymes. Taken together, both analyzed fluoroquinolones induced oxidative stress in melanocytes, however, the molecular and biochemical studies indicated the miscellaneous mechanisms for the tested drugs. The variability in phototoxic potential between lomefloxacin and moxifloxacin may result from different effects on the antioxidant enzymes.

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Oxidative Stress and Reproductive Function in the Aging Male

Biology ◽

10.3390/biology9090282 ◽

2020 ◽

Vol 9 (9) ◽

pp. 282

Author(s):

Paulina Nguyen-Powanda ◽

Bernard Robaire

Keyword(s):

Oxidative Stress ◽

Antioxidant Defense ◽

Structural Integrity ◽

Germ Line ◽

Reproductive Function ◽

Antioxidant Defense System ◽

Male Reproduction ◽

Paternal Age ◽

Theories Of Aging ◽

The Impact

With the delay of parenthood becoming more common, the age at which men father children is on the rise. While the effects of advanced maternal age have been well documented, only recently have studies started to focus on the impact of advanced paternal age (APA) in the context of male reproduction. As men age, the antioxidant defense system gradually becomes less efficient and elevated levels of reactive oxygen species (ROS) accumulate in spermatozoa; this can impair their functional and structural integrity. In this review, we present an overview of how oxidative stress is implicated in male reproductive aging by providing a summary of the sources and roles of ROS, the theories of aging, and the current animal and human studies that demonstrate the impacts of APA on the male germ line, the health of progeny and fertility, and how treatment with antioxidants may reverse these effects.

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Nickel Oxide Nanoparticles Induce Oxidative Stress and Morphological Changes on Marine Chlorella vulgaris

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.955-959.956 ◽

2014 ◽

Vol 955-959 ◽

pp. 956-960 ◽

Cited By ~ 2

Author(s):

Ning Gong ◽

Kui Shuang Shao ◽

Guang Yao Li ◽

Ye Qing Sun

Keyword(s):

Oxidative Stress ◽

Chlorella Vulgaris ◽

Antioxidant Defense ◽

Morphological Changes ◽

Antioxidant Defense System ◽

Ros Generation ◽

Nickel Oxide Nanoparticles ◽

Sod Activity ◽

Transmission Electron ◽

The Status

The status of oxidative stress of marineChlorella vulgariswas investigated via measuring the content of H2O2, MDA, SOD and CAT in cells after 72h NiO nanoparticles (nNiO) exposure. Morphological changes of algal cells were also determined by transmission electron microscopy (TEM). The results showed that nNiO induced the ROS generation and stimulated the antioxidant defense system of algae. Significant increases (p < 0.01) in MDA level and SOD activity were found after 72h 10 mg L-1nNiO treatment. H2O2content and CAT activity also increased under higher concentration treatments although non-significant. The disruption of plasma membrane and the disordered thylakoid lamella of algal cells were found under nNiO exposure, which indicated cell morphological changes. Our results implied that oxidative damage was one of toxic causes of nanoparticles on algae. It also indicates the potential impacts on aquatic biota by nanomaterials.

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Redox-dependent and independent mechanisms of selective pro- and anti-apoptotic activity of Georgian legumes crops extracts on Jurkat and MDCK cells

Functional Foods in Health and Disease ◽

10.31989/ffhd.v9i5.602 ◽

2019 ◽

Vol 9 (5) ◽

pp. 357

Author(s):

Tamar V. Sanikidze ◽

Tinatin L. Maminaishvili ◽

Nana V. Kipiani ◽

Maia G. Enukidze ◽

Marine G. Machavariani ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Defense ◽

Mdck Cells ◽

Antioxidant Defense System ◽

Stress Conditions ◽

Jurkat Cells ◽

Sod Activity ◽

Legume Crop ◽

Wide Range ◽

Apoptotic Activity

Background: Possibilities of pharmacological regulation of apoptosis play an important role in the treatment of different diseases. Polyphenol-rich plant extracts, as well as isolated polyphenols, can regulate cell apoptosis primarily through intrinsic and extrinsic mechanisms of action and are the most intriguing and studied class of compounds that can be therapeutics for a wide range of common diseases, including cancer. Polyphenols are well known as powerful antioxidants, their action is also associated with pro-apoptotic function in various types of tumor cells.The purpose of this study was to establish the anti-and pro-apoptotic activity of Georgian legume crops in model cellular systems.Methods: Legume crop extracts (LCEs) were prepared in water-alcohol solute. Polyphenols content in the extracts was determined by Folin–Ciocalteu method, antiradical activity (AA) - according the comparative time of the 50% neutralization of 2,2-diphenyl-1-picrylhydrazyl (DPPH) cleavage.Studies were carried out on human leukemic mature T (Jurkat) and normal epithelial MDCK cells lines. For modelling of oxidative stress, 30% hydrogen peroxide was used. LCEs were added to intact or incubated under oxidative stress conditions Jurkat and MDCK cells. Cells’ viability was determined by 3-(4,5-dimethyltiazol-2)-2,5-diphenyl-tetrazolium-bromide (MTT) test. Catalase and Superoxidedismutase (SOD) activity in cellular supernatant was measured by spectrophotometry.Results: LCEs revealed selective pro- and antiapoptotic activity on the intact and incubated under oxidative stress conditions Jurkat and MDCK cells. The cytotoxic effect of LCEs on intact Jurkat and MDCK cells was independent of their AA and activity of enzymatic cellular antioxidant defense system.The cytoprotective effect of LCEs on MDCK cells was realized through redox-dependent mechanisms and is associated both with the own AA of the extracts and with the stimulating effect of the extracts on the activity of enzymatic cellular antioxidant defense system, where catalase played a leading role. LCEs didn’t protect Jurkat cells from oxidative stress-induced apoptosis.Conclusion: The obtained results allow us to conclude that in cancer chemotherapy the legume extracts might be combined with prooxidant drugs, in order to protect normal cells, but not the malignant ones, from their apoptosis-inducing effect. On the other hand, these extracts may protect non-malignant tissues/organs from various apoptosis-related disorders.

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Skeletal muscle uncoupling-induced longevity in mice is linked to increased substrate metabolism and induction of the endogenous antioxidant defense system

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00518.2012 ◽

2013 ◽

Vol 304 (5) ◽

pp. E495-E506 ◽

Cited By ~ 26

Author(s):

S. Keipert ◽

M. Ost ◽

A. Chadt ◽

A. Voigt ◽

V. Ayala ◽

...

Keyword(s):

Oxidative Stress ◽

Skeletal Muscle ◽

Antioxidant Defense ◽

Antioxidant Defense System ◽

Mapk Signaling ◽

Redox Signaling ◽

Defense System ◽

High Fat ◽

Substrate Metabolism ◽

Endogenous Antioxidant

Ectopic expression of uncoupling protein 1 (UCP1) in skeletal muscle (SM) mitochondria increases lifespan considerably in high-fat diet-fed UCP1 Tg mice compared with wild types (WT). To clarify the underlying mechanisms, we investigated substrate metabolism as well as oxidative stress damage and antioxidant defense in SM of low-fat- and high-fat-fed mice. Tg mice showed an increased protein expression of phosphorylated AMP-activated protein kinase, markers of lipid turnover (p-ACC, FAT/CD36), and an increased SM ex vivo fatty acid oxidation. Surprisingly, UCP1 Tg mice showed elevated lipid peroxidative protein modifications with no changes in glycoxidation or direct protein oxidation. This was paralleled by an induction of catalase and superoxide dismutase activity, an increased redox signaling (MAPK signaling pathway), and increased expression of stress-protective heat shock protein 25. We conclude that increased skeletal muscle mitochondrial uncoupling in vivo does not reduce the oxidative stress status in the muscle cell. Moreover, it increases lipid metabolism and reactive lipid-derived carbonyls. This stress induction in turn increases the endogenous antioxidant defense system and redox signaling. Altogether, our data argue for an adaptive role of reactive species as essential signaling molecules for health and longevity.

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Impact of seasonally frozen soil on germinability and antioxidant enzyme activity of Picea asperata seeds

Canadian Journal of Forest Research ◽

10.1139/x09-001 ◽

2009 ◽

Vol 39 (4) ◽

pp. 723-730 ◽

Cited By ~ 3

Author(s):

Jihong Qin ◽

Qing Liu

Keyword(s):

Oxidative Stress ◽

Antioxidant Defense ◽

Germination Rate ◽

Antioxidant Defense System ◽

Antioxidant Enzyme Activity ◽

Frozen Soil ◽

Defense System ◽

Frozen Ground ◽

Subalpine Zone ◽

Picea Asperata

In the subalpine zone of the Qinghai–Tibetan Plateau of China, Dragon spruce (Picea asperata Mast.) is commonly used for reforestation. The aim of the present work was to study the effects of seasonally frozen soil on the germination of P. asperata seeds and to investigate whether these effects were associated with resumption of the antioxidant defense system. The nonfrozen treatment resulted in near failure of germination (1%) and was associated with relatively high levels of hydrogen peroxide (H2O2) and low activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxide (APX). Germination of P. asperata seeds at 10 cm under the seasonally frozen soil was higher than that at 5 cm by 26%; this higher germination rate was associated with the recovery of SOD, CAT, and APX activities. The levels of malondialdehyde (MDA) in seeds from seasonally frozen treatments were higher than those in the nonfrozen treatment, implying greater lipid peroxidation and that frozen seeds might have suffered from oxidative stress. The results indicate that seasonally frozen soil facilitated the germination of P. asperata seeds and that germination was closely related to the resumption of antioxidant enzymes activity. Overall, these findings suggest that the disappearance of seasonally frozen ground caused by global warming might result in failure of regeneration of P. asperata.

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Enriched environment prevents oxidative stress in zebrafish submitted to unpredictable chronic stress

PeerJ ◽

10.7717/peerj.5136 ◽

2018 ◽

Vol 6 ◽

pp. e5136 ◽

Cited By ~ 8

Author(s):

Matheus Marcon ◽

Ricieri Mocelin ◽

Adrieli Sachett ◽

Anna M. Siebel ◽

Ana P. Herrmann ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Chronic Stress ◽

Brain Homogenate ◽

Enriched Environment ◽

Laboratory Animals ◽

Sod Activity ◽

Zebrafish Brain ◽

Positive Effects ◽

The Impact

Background The enriched environment (EE) is a laboratory housing model that emerged from efforts to minimize the impact of environmental conditions on laboratory animals. Recently, we showed that EE promoted positive effects on behavior and cortisol levels in zebrafish submitted to the unpredictable chronic stress (UCS) protocol. Here, we expanded the characterization of the effects of UCS protocol by assessing parameters of oxidative status in the zebrafish brain and reveal that EE protects against the oxidative stress induced by chronic stress. Methods Zebrafish were exposed to EE (21 or 28 days) or standard housing conditions and subjected to the UCS protocol for seven days. Oxidative stress parameters (lipid peroxidation (TBARS), reactive oxygen species (ROS) levels, non-protein thiol (NPSH) and total thiol (SH) levels, superoxide dismutase (SOD) and catalase (CAT) activities were measured in brain homogenate. Results Our results revealed that UCS increased lipid peroxidation and ROS levels, while decreased NPSH levels and SOD activity, suggesting oxidative damage. EE for 28 days prevented all changes induced by the UCS protocol, and EE for 21 days prevented the alterations on NPSH levels, lipid peroxidation and ROS levels. Both EE for 21 or 28 days increased CAT activity. Discussion Our findings reinforce the idea that EE exerts neuromodulatory effects in the zebrafish brain. EE promoted positive effects as it helped maintain the redox homeostasis, which may reduce the susceptibility to stress and its oxidative impact.

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The role of the antioxidant: Selenopyran in the stability of the antioxidant system to toxic effect of cadmium chloride

Biotechnology in Animal Husbandry ◽

10.2298/bah0701181k ◽

2007 ◽

Vol 23 (5-6-1) ◽

pp. 181-191

Author(s):

U. Kravchenko ◽

G. Borjaev ◽

M. Nevitov ◽

A. Ostapchuk ◽

E. Kistanova

Keyword(s):

Oxidative Stress ◽

Antioxidant System ◽

Antioxidant Defense ◽

Age Groups ◽

Selenium Concentration ◽

Antioxidant Defense System ◽

The Stability ◽

Gpx Activity ◽

Preventive Role

The purpose of the present work was, under conditions of the model experiment on rats, to tap the information about the features of shortterm acclimatization of the antioxidant system in various organs to toxicity of cadmium at stages of an ontogenesis and about the preventive role of the antioxidant selenopyran in this process. The obtained results showed the ontogenetic differences in the adaptive reactivity of the enzymatic antioxidant defense system in the most important organs and tissues of rats under conditions of oxidative stress induced by cadmium. The ontogenetic differences of Se redistribution in a body under influence of cadmium administration were found. The discovered decrease of Se concentration in the liver of young animals and the increase of its concentration in the liver of old animals correlated positively with the changes of GPx activity. Preventive administration of selenopyran (9- phenyl-simmetrical octa-hydroselenoxanthene) to old animals reduced the oxidative stress intensity. Animals of all age groups showed higher selenium concentration in the tissues and the increase in the selenium-dependent GPx activity.

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