scholarly journals Single Dose of N-Acetylcysteine in Local Anesthesia Increases Expression of HIF1α, MAPK1, TGFβ1 and Growth Factors in Rat Wound Healing

2021 ◽  
Vol 22 (16) ◽  
pp. 8659
Author(s):  
Wiktor Paskal ◽  
Michał Kopka ◽  
Albert Stachura ◽  
Adriana M. Paskal ◽  
Piotr Pietruski ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Regenerative Processes ◽  
Wound Site ◽  
Post Surgery ◽  
Sprague Dawley Rats ◽  
Anesthetic Solution

In this study, we aimed to investigate the influence of N-acetylcysteine (NAC) on the gene expression profile, neoangiogenesis, neutrophils and macrophages in a rat model of incisional wounds. Before creating wounds on the backs of 24 Sprague–Dawley rats, intradermal injections were made. Lidocaine–epinephrin solutions were supplemented with 0.015%, 0.03% or 0.045% solutions of NAC, or nothing (control group). Scars were harvested on the 3rd, 7th, 14th and 60th day post-surgery. We performed immunohistochemical staining in order to visualize macrophages (anti-CD68), neutrophils (anti-MPO) and newly formed blood vessels (anti-CD31). Additionally, RT-qPCR was used to measure the relative expression of 88 genes involved in the wound healing process. On the 14th day, the number of cells stained with anti-CD68 and anti-CD31 antibodies was significantly larger in the tissues treated with 0.03% NAC compared with the control. Among the selected genes, 52 were upregulated and six were downregulated at different time points. Interestingly, NAC exerted a significant effect on the expression of 45 genes 60 days after its administration. In summation, a 0.03% NAC addition to the pre-incisional anesthetic solution improves neovasculature and increases the macrophages’ concentration at the wound site on the 14th day, as well as altering the expression of numerous genes that are responsible for the regenerative processes.

scholarly journals N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study

2021 ◽  
Vol 22 (14) ◽  
pp. 7549
Author(s):  
Wiktor Paskal ◽  
Adriana M. Paskal ◽  
Piotr Pietruski ◽  
Albert Stachura ◽  
Kacper Pełka ◽  
...  
Keyword(s):  
Wound Healing ◽  
Early Stage ◽  
Healing Process ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Time Points ◽  
Model Study ◽  
Sprague Dawley Rats ◽  
Animal Model Study ◽  
Anesthetic Solution

The aim of the study was to evaluate if a pre-incisional N-acetylcysteine (NAC) treatment altered the process of wound healing in a rat model. The dorsal skin of 24 Sprague-Dawley rats was incised in six locations. Before the incisions were made, skin was injected either with lidocaine and epinephrine (one side) or with these agents supplemented with 0.015%, 0.03%, or 0.045% NAC (contralaterally). Photographic documentation of the wound healing process was made at 11 time points. Rats were sacrificed 3, 7, 14, or 60 days after incision to excise scars for histological analysis. They included: Abramov scale scoring, histomorphometry analysis, and collagen fiber arrangement assessment. Skin pretreated with 0.03% NAC produced the shortest scars at all analyzed time points, though this result was statistically insignificant. At this NAC concentration the scars had smaller areas on the third day and were narrower on the day 4 compared with all the other groups (p < 0.05). On day 7, at the same concentration of NAC, the scars had a higher superficial concentration index (p = 0.03) and larger dermal proliferation area (p = 0.04). NAC addition to pre-incisional anesthetic solution decreased wound size and width at an early stage of scar formation at all concentrations; however, with optimal results at 0.03% concentration.

scholarly journals Efektivitas getah batang betadine (Jatropha multifida L.) terhadap penyembuhan luka pasca pencabutan gigi pada tikus Sprague-DawleyEffectiveness of betadine (Jatropha multifida L.) stem sap on the wound healing after tooth extraction in Sprague-Dawley rats

2021 ◽  
Vol 33 (2) ◽  
pp. 145
Author(s):  
Hendry Rusdy ◽  
Astri Suryani Pasaribu Saruksuk ◽  
Rahmi Syaflida Dalimunte ◽  
Gostry Aldica Dohude
Keyword(s):  
Wound Healing ◽  
Tranexamic Acid ◽  
Tooth Extraction ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Jatropha Multifida ◽  
Plant Sap

Pendahuluan: Pencabutan gigi merupakan prosedur yang sering dilakukan di kedokteran gigi. Setelah pencabutan gigi akan dihasilkan suatu perlukaan. Proses penyembuhan luka dapat dipercepat pada kondisi tertentu. Salah satu bahan alami yang dapat membantu proses penyembuhan luka adalah getah tanaman betadine (Jatropha multifida L.). Penelitian bertujuan untuk menganalisis efektivitas getah tanaman betadine terhadap penyembuhan luka dan terhadap tanda-tanda infeksi pasca pencabutan gigi Metode: Desain penelitian studi eksperimental laboratorium dengan rancangan penelitian post-test only control group design menggunakan 30 ekor tikus Sprague-Dawley. Teknik pengambilan sampel yaitu convenience sampling. Sampel dibagi menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol. Kelompok perlakuan diberikan getah tanaman betadine dan kelompok kontrol diberikan asam traneksamat secara oral menggunakan sonde lambung. Tunggu selama 4 jam setelah pemberian getah tanaman betadine dan asam traneksamat. Anestesi pada tikus menggunakan ketamin 50 mg/kg berat badan secara intramuskular kemudian dilakukan pencabutan pada gigi tikus. Pengamatan dilakukan dengan melihat kriteria klinis pada hari 1,3,7 dan diperhatikan sampai luka sembuh serta lihat tanda-tanda infeksi. Analisis data dilakukan dengan uji normalitasShapiro Wilik. Hasil penelitian menunjukkan bahwa data berdistribusi tidak normal. Analisis data dilanjutkan menggunakan uji statistik mann whitney. Hasil: Terdapat perbedaan signifikan penyembuhan luka soket pasca pencabutan gigi setelah diberikan getah betadine dan asam traneksamat dengan nilai p=0,037 (p<0,005). Simpulan: Pemberian getah tanaman betadine terbukti lebih efektif terhadap proses penyembuhan luka soket pasca pencabutan gigi dibandingkan dengan pemberian asam traneksamat. Kata kunci: tikus Sprague-Dawley; penyembuhan luka; pencabutan gigi; getah batang betadine ABSTRACTIntroduction: Tooth extraction is a procedure often performed in dentistry. Tooth extraction will always cause injuries. However, the wound healing process can be accelerated under certain conditions. One of the natural ingredients that can accelerate the wound healing process is betadine (Jatropha multifida L.) plant sap. The study was aimed to analyzed the effect of betadine plant sap on wound healing and signs of infection after tooth extraction. Methods: Experimental laboratory study design with post-test only control group design was conducted towards 30 Sprague-Dawley rats. The sampling technique was convenience sampling. The sample was divided into two groups, the treatment group and the control group. The treatment group was administered with betadine plant sap, and the control group was administered with tranexamic acid orally using a gastric probe, then waited 4 hours after. The anaesthesia was then performed using 50 mg/kg body weight of ketamine intramuscularly. The extraction was performed after. Observations was conducted at the clinical criteria on days 1, 3, and 7 and continue to be monitored until the wound heals. Then, the signs of infection were observed. Data analysis was carried out using the Shapiro Wilk normality test. The results showed that the data was not normally distributed. Thus, data analysis was continued using the Mann Whitney statistical test. Results: The results showed a significant difference in the healing of socket wounds after tooth extraction after being administered with betadine sap and tranexamic acid with a value of p=0.037 (p<0.005). Conclusions: Administration of betadine plant sap is proven to be more effective in accelerating the healing process of socket wounds after tooth extraction than tranexamic acid. Keywords: Sprague-Dawley rats; wound healing; pencabutan gigi; getah batang betadine 

scholarly journals Pengaruh Aplikasi Gel Ekstrak Kulit Citrus Sinensis terhadap Epitelisasi pada Penyembuhan Luka Gingiva Tikus Sprague Dawley

2015 ◽  
Vol 1 (1) ◽  
pp. 86
Author(s):  
Aqilla Tiara Kartiningtyas ◽  
Prayitno Prayitno ◽  
Sri Pramestri Lastianny
Keyword(s):  
Wound Healing ◽  
Sweet Orange ◽  
Healing Process ◽  
Orange Peel ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Orange Peel Extract ◽  
Peel Extract

Penyembuhan luka merupakan mekanisme pertahanan jaringan, dengan epitelisasi sebagai salah satu parameternya. Kulit jeruk manis memiliki potensi dalam penyembuhan luka karena mengandung nutrisi yang memiliki peran dalam mempercepat penyembuhan luka. Penelitian ini bertujuan untuk mengetahui efek aplikasi topikal gel ekstrak kulit jerukmanis terhadap epitelisasi pada proses penyembuhan luka gingiva labial tikus Sprague Dawley. Dua puluh tujuh ekor tikus Sprague Dawley, berumur 2-3 bulan, dibagi dalam 3 kelompok, kontrol positif, perlakuan, dan kontrol negatif. Perlukaan pada gingiva labial mandibula dibuat dengan punch biopsy berdiameter 2,5 mm. Luka pada kelompok kontrolpositif diaplikasikan Aloclair, kelompok perlakuan diaplikasikan gel ekstrak kulit jeruk manis 10%, dan kelompok kontrol negatif diaplikasikan CMC-Na, masing-masing 2 kali sehari selama 1 menit secara topikal. Tiga ekor tikus dari tiap kelompok didekapitasi masing-masing pada hari ke-3, 7, dan 14. Jaringan luka diambil dan dibuat sediaan histologis dengan pengecatan Hemaktosilin Eosin. Pengukuran ketebalan epitel dilakukan dengan menggunakan Optilab yang dipasang pada mikroskop cahaya. Data ketebalan epitel dianalisis dengan menggunakan ANAVA dua jalur. Hasil pengukuran ketebalan epitel menunjukkan perbedaan yang tidak signifikan pada kelompok kontrol positif dengan perlakuan (p>0,05), namun menunjukkan perbedaan yang signifikan pada kelompok perlakuan dengan kontrol negatif (p<0,05) pada hari 3, 7, dan 14 setelah perlukaan. Kesimpulan dari penelitian ini adalah aplikasi topikal gel ekstrak kulit jeruk manis mempercepat epitelisasi pada proses penyembuhan luka gingiva labial tikus Sprague Dawley. Effect of Topical Application of Sweet Orange (Citrus sinensis) Peel Extract Gel on Epithelialization of Labial Gingival Wound Healing: In Vivo Studies in Sprague Dawley Rats. Wound healing is a defense mechanism from complex biological phenomenon, in which epithelialization occurs as one of its parameters. Sweet orange peelcontains nutrients that have role in enhancing wound healing process. The objective of this research is to determine the effect of topical application of sweet orange peel extract gel on epithelialization of wound healing process in labial mandibular gingiva of Sprague Dawley rats. Twenty seven Sprague Dawley rats were divided into 3 groups: positivecontrol, treatment, and negative control. Labial mandibular gingival was wounded using 2.5 mm diameter punch biopsy. Topically, each wound of positive control group was administered Aloclair, treatment group was administered 10% sweet orange peel extract gel, and negative control group was administered CMC-Na, twice a day for 1 minute. Three rats from each group were sacrificed for histological evaluation at 3, 7, and 14 days and the specimens were stained with HE. The measurement of the epithelial thickness used Optilab installed on microscope. The data obtained from the measurement was analyzed using two-way ANOVA. There was insignificant difference between positive control group and treatment group (p>0.05) while there was significant difference between treatment group and negative control group (p<0.05) observed at 3, 7, and 14 days. The conclusion of this research is that the application of sweet orange peel extract gel accelerates epithelialization of wound healing process in labial mandibular gingiva of Sprague Dawley rats.

scholarly journals Effects of Albumin Infusion on Serum Levels of Albumin, Proinflammatory Cytokines (TNF-α, IL-1, and IL-6), CRP, and MMP-8; Tissue Expression of EGRF, ERK1, ERK2, TGF-β, Collagen, and MMP-8; and Wound Healing in Sprague Dawley Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Arie Utariani ◽  
Eddy Rahardjo ◽  
David S. Perdanakusuma
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Serum Levels ◽  
Tissue Expression ◽  
Wound Healing Process ◽  
Casein Diet ◽  
Sprague Dawley ◽  
Albumin Infusion ◽  
Sprague Dawley Rats ◽  
Protein Feed

In this study, we sought to determine the roles of albumin in wound healing, which is infused both pre- and postoperatively in malnourished patients presenting with hypoalbuminemia. For the purposes of the study, we used 25 male Sprague Dawley rats of predetermined weight and age, which were initially maintained in a standard environment and fed the same diet for 7 days prior to being segregated into one of the following five groups: A, control, normal protein feed (20% casein); B, hypoalbuminemia, 25% rat albumin infusion prior to surgery; C, hypoalbuminemia, normal protein feed (20% casein); D, hypoalbuminemia, 25% rat albumin infusion after surgery; and E, hypoalbuminemia, low-protein feed (casein 2%). The animals in all five groups were subjected to four deep incisions in their dorsal muscle fascia. On days 1, 3, 5, and 7 after surgery, ELISA was used to determine serum levels of TNF-α, IL-1, IL-6, CRP, and MMP-8, whereas immunohistochemistry was used to determine the tissue expression of EGFR, ERK1, ERK2, TGF-β, collagen, and MMP-8. Significant reductions in serum levels of TNF-α, IL-1, and CRP were detected in the groups receiving albumin infusion and the high-casein diet (P<0.05). The administration of albumin and a high-casein diet also increased the tissue expression of EGFR, ERK1, ERK2, TGF-β, and collagen and decreased that of MMP-8 relative to the hypoalbuminemia control (P<0.05). We propose that the administration of albumin promoted NF-κB signaling which, in turn, induced the transduction and transcription of factors involved in wound healing. Albumin infusion and dietary proteins play vital roles in accelerating the wound healing process, as they can contribute to correcting the hypoalbuminemic state. These findings provide insights that will contribute to our understanding of wound healing, particularly in malnourished patients.

scholarly journals The Effect Of Erythropoietin Administration In Experimental Burns Wound Healing: An Animal Study

2016 ◽  
Vol 3 (1) ◽  
pp. 1-8
Author(s):  
Afriyanti Sandhi ◽  
Aditya Wardhana
Keyword(s):  
Wound Healing ◽  
Animal Study ◽  
Low Dose ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
High Dose ◽  
Sprague Dawley ◽  
Surface Areas

Background: The hematopoietic growth factor erythropoietin (EPO) attracts attention due to its all-tissue-protective pleiotropic properties. The purpose of this study is to investigate the effect of EPO in experimental burn wounds healing. Methods: Fifteen healthy Sprague-Dawley, strain of Rattus Novergicus weighing 300-350 grams, were prepared to achieve deep dermal burns. Animals were randomized to receive either low-dose EPO injection (600 IU/mL), high-dose EPO injection (3000 IU/mL) or nothing (control group). After 14 days of observations, quantitative and qualitative assessments of wound healing was determined. Results: The size of the wound area and re-epithelialization rate percentage was determined on Day-0, Day-5, Day-10, and Day-14. The average of raw surface areas measurement (p value: 0.012 in day-5; 0.009 in day-10 and 0.000 in day-14) and healing percentage of the lesions (p value: 0.011 in day-5; 0.016 in day-10 and 0.010 in day-14) were significantly best in the low-dose EPO grup compared to the control group and high-dose EPO grup. The histopathology evaluation revealed that the highest score for for re-epithelialization, granulation tissue and neo-angiogenesis were achieved by the low-dose EPO injection group than in both control and high-dose EPO injection groups. Conclusion: In this animal study using Sprague-Dawley rats, Recombinant Human EPO (rHuEPO) injection administration prompted the evidences of improved re-epithelialization and wound healing process of the skin caused by deep dermal burns. These findings may lead to a new therapeutic approach to improve the clinical outcomes for the management of burns wound healing.

scholarly journals Development and In Vivo Characterization of Probiotic Lysate-Treated Chitosan Nanogel as a Novel Biocompatible Formulation for Wound Healing

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Yousef Ashoori ◽  
Milad Mohkam ◽  
Reza Heidari ◽  
Seyedeh Narjes Abootalebi ◽  
Seyyed Mojtaba Mousavi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Bacillus Subtilis ◽  
Lactobacillus Reuteri ◽  
Healing Process ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Valuable Compounds ◽  
In Vivo Characterization

Wound healing is a physiological reaction to tissue injuries which plays a crucial role in replacing the destroyed tissues. Probiotics produce valuable compounds that possess antibacterial and anti-inflammatory activities, immunomodulatory effects, and angiogenesis traits leading to the promotion of wound healing. Chitosan nanostructures have versatile properties making them quickly produced into gels, scaffolds, nanoparticles, beads, and sponge structures that can be incorporated into wound healing processes. In the current study, three formulations from nanogel consisting of probiotic supernatant (Lactobacillus reuteri, Lactobacillus fermentum, and Bacillus subtilis sp. natto)-loaded chitosan nanogels were prepared from the culture of corresponding cultures. The chitosan nanogels were previously characterized by Zetasizer, FTIR, and TEM. The prepared formulations’ effectiveness and dressing activity were assessed by evaluating wound closure and histological trials in Sprague-Dawley rats. The results indicated that all probiotic lysate formulations have advantages over the wound healing process. However, Bacillus subtilis natto has a better wound healing quality, which is well known in pathology examination. The favorable effects of probiotic lysate nanogels, including the reasonable wound closing rate, good wound appearance, and satisfactory histological observation via in vivo examination, suggest it as a promising nominee for wound healing purposes.

scholarly journals Effect of topical application of fibronectin in duodenal wound healing in rats

2003 ◽  
Vol 18 (2) ◽  
pp. 97-101
Author(s):  
Teresa Neuma de Souza Brito ◽  
Luiz Reginaldo Menezes da Rocha ◽  
Carlos André Nunes Jatobá ◽  
Maurício Pereira Sales ◽  
Aldo da Cunha Medeiros
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Test Group ◽  
Large Family ◽  
Repair Process ◽  
Tissue Level ◽  
Control Group ◽  
Wound Healing Process ◽  
Wound Site ◽  
Hematoxylin Eosin

Fibronectin (FN), a large family of plasma and extracellular matrix glycoproteins, plays an important role in wound healing. PURPOSE: To evaluate the effect of fibronectin on the healing of sutured duodenal wounds, correlating with the serum and tissue level of the substance. METHODS: An experimental study was done in 30 adult Wistar rats divided into two group. In the control group (n=15) a duodenal suture was treated with saline solution 0,9% and in the test group the duodenal wounds were treated with 1% FN. The duodenal wound healing process was studied in the 5th, 7tn and 10th postoperative days, by histological sections stained by hematoxylin-eosin, Masson trichromic and immunohistochemical reaction for FN. A digital histological grading system was used to obtain a score for each group and to observe the healing process. RESULTS: the FN was present in the several layers of the duodenum and the cellular and plasmatic FN increased with the evolution of healing. In the test group the FN enhanced the wound healing within 5, 7 and 10 days after injury, when compared with the control group. CONCLUSION: The topical use of FN in duodenal sutured wounds in rats enhances healing by stimulating the appearence of fibroblasts into the wound site and development of granulation tissue. This acceleration of the repair process may have an important application in the healing of duodenal wounds.

scholarly journals Expression of fibroblast growth factor-2 after application of the Queen's crepe-myrtle leaf (Lagerstroemia speciosa) and aloe vera extract gel in the wound healing process of hyperglycemic

2021 ◽  
Vol 33 (1) ◽  
pp. 48
Author(s):  
Fachrul Razi ◽  
Andri Hardianto ◽  
Lucky Riawan ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Aloe Vera ◽  
Simple Random Sampling ◽  
Fibroblast Growth Factor 2 ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Lagerstroemia Speciosa ◽  
Significant Difference

Introduction: Fibroblast Growth Factor-2 (FGF-2) have a role in stimulating the proliferation and migration of various types of cells in the wound healing process. Hyperglycemic conditions can disrupt the wound healing process. Lagerstroemia speciosa are known to have antyphyerglycemic and antioxidant effects. Aloe vera is a plant that has been used for long time in topical treatment of wounds.This study was aimed to analyse the FGF-2 expressions on the wound healing process of subjects with hyperglycemia after application of Queen's crepe-myrtle leaf (Lagerstroemia speciosa) and aloe vera extracts gel. Methods: A pure experimental study was conducted with simple random sampling. The sample was 27 experimental animals (CI 95%; power test 80%) of Sprague Dawley rat induced to have a hyperglycemic state using alloxan and had their palate injured. The immunohistochemical examination was performed to assess FGF-2 expression level. Samples were divided into three groups with a simple random sampling technique: nine of the rats received the Lagerstroemia speciosa extract gel application; nine rats received the aloe vera extract gel application on the injured palate; another nine rats in the control group. Examination of the FGF-2 expression level was performed on the third, seventh, and fourteenth observation days. Results: Overall, the control group had a significant difference with the Lagerstroemia speciosa and aloe vera extract gel group (p-value<0.05), however, the Lagerstroemia speciosa group did not have any significant difference with the aloe vera group (0.123>0.05). Conclusion: There was no difference in the amount of FGF-2 expressions on the wound healing process of the injured palatal tissue of Sprague-Dawley rats with hyperglycemic after application of the Lagerstroemia speciosa and aloe vera extract gel.

scholarly journals EFEK EKSTRAK TANAMAN ASHITABA PADA KADAR SERUM ALKALIN FOSFATASE (ALP) PADA TIKUS YANG TERPAPAR LUKA BAKAR

2021 ◽  
Vol 5 (1) ◽  
pp. 26-34
Author(s):  
Andrew Johan ◽  
Kusmiyati Tjahjono ◽  
Wenda Yoanda ◽  
Dwi Ngestiningsih ◽  
Lusiana Batubara
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Serum Alkaline Phosphatase ◽  
Healing Process ◽  
T Test ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Control Group Design

Serum alkaline phosphatase (ALP) levels increases in burn tissue damage. Several bioactive compounds found in Ashitaba, are expected to reduce serum ALP levels and enhance the process of wound healing. This study aims to prove that administration of oral Ashitaba extract can reduce serum ALP levels in rats exposed to burn trauma. This study uses true experimental post-test control group design with a total of 20 Sprague dawley rats as samples. All samples were inflicted with 2nd degree burn wound and divided into 2 groups, treatment group (Ashitaba extract 300 mg/KgBW) and control group. Blood serum were analyzed for ALP levels on the 2nd, 8th and 14th days. Kinetic-IFCC method was used to find serum ALP levels. Data was analyzed using Mann-Whitney Test, paired T-test amd independence T-test. Scalded wound size was measured macroscopically over the course of 21 days to find contracture rate. In conclusion, Ashitaba extract is not proven to significantly reduce the serum ALP levels, increase contracture rate and enhance burn wound healing process. However, there was slight increase in contracture rate in treatment group as compared to control group. In addition, there was a lower ALP levels in treatment group as compared to control group in day 8.

scholarly journals The efficacy taro leaf extract on wound healing contaminated with Staphylococcus aureus bacteria

2021 ◽  
Vol 33 (3) ◽  
pp. 199
Author(s):  
Endang Sjamsudin ◽  
Annisya Muharty ◽  
Lucky Riawan ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Treatment Group ◽  
Leaf Extract ◽  
Healing Process ◽  
Colocasia Esculenta ◽  
Control Group ◽  
Sprague Dawley ◽  
Group I ◽  
Sprague Dawley Rats

Introduction: taro leaves (Colocasia esculenta (L.) Schott) have been reported to possess wound healing, anti-inflammatory, antibacterial and antioxidant activities. One of the factors that affect wound healing is infection in the wound, wherein the wound is infected by  bacteria, especially Staphylococcus aureus that is mostly found in the oral cavity, the wound healing process will be hampered and become longer healed.The objective of this research was to analyze the efficacy of Colocasia esculenta (L.) Schott extract on the healing process of wounds contaminated with Staphylococcus aureus. Methods: This experimental study on Sprague dawley rats was carried out at the Teaching Veterinary Hospital, IPB . Twenty-four Sprague dawley rats were made with circular excision wounds with a diameter of 2 cm on the dorsum, and Staphylococcus aureus specimens were contaminated with a dose of 3.4x108 LAC. Rats were randomly divided into 2 groups. Group I was given a placebo, group II was given a 25% concentration of Colocasia esculenta (L.) Schott extract on the wound once a day. Four rats from each group were euthanized on days 3, 7, and 14. The number of neutrophils, macrophages, re-epithelialization and FGF-2 expression were measured by microscopic observation with visual field control, and comparative data were analyzed using the Mann-Whitney statistical test. Results: The number of neutrophils on day 7 of the Colocasia esculenta (L.) Schott leaf extract group was less than the control group (p=0.040). The number of macrophages on the 3rd day was more in the treatment group than the control group (p=0.032), and on the 14th day, the treatment group was less than the control group (p=0.040). Epithelial cells on the 14th day of the treatment group were more than the control group (p=.0.017). The level of FGF-2 expression on day 7 of the treatment group was higher than the control group (p=0.044). Conclusion: Application of extracts  Colocasia esculenta (L.) Schott leaf is proven to be efficacious on day 7 for wound healing contaminated with Staphylococcus aureus bacteria.

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