Comparison of the Effects of Resveratrol and Its Derivatives on the Radiation Response of MCF-7 Breast Cancer Cells

Radiotherapy is among the most important methods for breast cancer treatment. However, this method’s effectiveness is limited by radioresistance. The aim of this study was to investigate whether the stilbene derivatives piceid, resveratrol, and piceatannol have a radiosensitising effect on breast cancer cells (MCF-7). The conducted research enabled us to determine which of the tested compounds has the greatest potential in sensitising cells to ionising radiation (IR). Among the stilbene derivatives, resveratrol significantly increased the effect of IR. Resveratrol and IR used in combination had a higher cytotoxic effect on MCF-7 cells than using piceatannol, piceid, or radiation alone. This was due to a significant decrease in the activity of antioxidant enzymes, which resulted in the accumulation of formed reactive oxygen species (ROS). The effect of resveratrol and IR enhanced the expression of apoptotic genes, such as Bax, p53, and caspase 8, leading to apoptosis.

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Cytotoxic effect of Drimia maritima bulb extract and induction of mitochondrial apoptotic signaling in human breast cancer cells, MCF-7 and MDA-MB-468

OncoTargets and Therapy ◽

10.2147/ott.s182786 ◽

2018 ◽

Vol Volume 11 ◽

pp. 7669-7677 ◽

Cited By ~ 1

Author(s):

Maryam Hamzeloo-Moghadam ◽

Mahmoud Aghaei ◽

Mohammad Hossein Abdolmohammadi ◽

Amir Khalaj ◽

Faranak Fallahian

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Human Breast Cancer ◽

Breast Cancer Cells ◽

Cytotoxic Effect ◽

Human Breast Cancer Cells ◽

Human Breast ◽

Apoptotic Signaling ◽

Mcf 7

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Hydroxycitric acid potentiates the cytotoxic effect of tamoxifen in MCF-7 breast cancer cells through inhibition of ATP citrate lyase

Steroids ◽

10.1016/j.steroids.2020.108656 ◽

2020 ◽

Vol 160 ◽

pp. 108656 ◽

Cited By ~ 1

Author(s):

Ahmed Ismail ◽

Ahmed S. Doghish ◽

Bakheet E. M. Elsadek ◽

Salama A. Salama ◽

Amr D. Mariee

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Cytotoxic Effect ◽

Atp Citrate Lyase ◽

Hydroxycitric Acid ◽

Citrate Lyase ◽

Mcf 7

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Induction of cell cycle arrest in human MCF-7 breast cancer cells by cis-stilbene derivatives related to VIOXX®

Toxicology Letters ◽

10.1016/j.toxlet.2009.01.017 ◽

2009 ◽

Vol 186 (2) ◽

pp. 115-122 ◽

Cited By ~ 9

Author(s):

Sunisa Sangjun ◽

Esther de Jong ◽

Sandra Nijmeijer ◽

Thumnoon Mutarapat ◽

Somsak Ruchirawat ◽

...

Keyword(s):

Breast Cancer ◽

Cell Cycle ◽

Cell Cycle Arrest ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Cycle Arrest ◽

Stilbene Derivatives ◽

Mcf 7

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Anti-carcinogenic effect of Cathepsin K Inhibitor, Odanacatib with low dose of Cisplatin Against Human Breast Carcinoma MCF-7 and MDAMB231 Cells

Current Cancer Therapy Reviews ◽

10.2174/1573394716666201222101925 ◽

2020 ◽

Vol 16 ◽

Author(s):

Yaongamphi Vashum ◽

Amuthavalli Kottaiswamy ◽

Tholcopiyan Loganathan ◽

Fathima Bushra Sheriff ◽

Shila Samuel

Keyword(s):

Breast Cancer ◽

Protein Expression ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Cytotoxic Effect ◽

Low Dose ◽

Cathepsin K ◽

Platinum Compound ◽

Alternative Treatment Option ◽

Mcf 7

Background: A cross-linking agent commonly used for cancer chemotherapy is a platinum compound such as cisplatin. However, with the acquisition of cellular drug resistance and adverse side effects, the potency of cisplatin is therefore, often tempered. To overcome these, the present study has established the use of cathepsin k (CTSK) inhibitor as a potent chemo sensitizer. Methods: The cytotoxic effect of cisplatin and odanacatib (ODN) on two different breast cancer patient-derived cell lines, MDA-MB-231 and MCF-7, was assessed by MTT-based colorimetric assay. The drug interaction coefficient CDI was used to evaluate the synergistically inhibitory impact of the drug combination and immunoblot was used to examine protein expression of certain proteins responsible for cell survival and the mechanism of apoptosis. Results: In this study, we found that IC50 of ODN in combination with cisplatin (half of IC25) induces a synergistic cytotoxic effect in different breast cancer cells. Diminished expression of Bcl-2 and increased expression of Bax aroused the cytochrome release, that triggers caspase-9 and -3 activation in the combinatorial group. ODN with lower dose of cisplatin significantly inhibits the protein expression of novel chemoresistant factors such as STAT3, NFҡB and IL-6. Conclusion: This study highlights the potential effects of the combination of ODN with reduced dose of cisplatin on improving the growth inhibition and apoptosis-inducing effect on breast cancer cells via combined inhibition of NF-κBinduced IL-6 and STAT3 activation.The study result suggests that the further development of this novel inhibitor combination with low dose of standard cisplatin-based chemotherapy may contribute to alternative treatment option for certain cancers.

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Synergistic cytotoxic effects of tamoxifen and black cohosh on MCF-7 and MDA-MB-231 human breast cancer cells: an in vitro studyThis article is one of a selection of papers published in this special issue (part 2 of 2) on the Safety and Efficacy of Natural Health Products.

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y07-111 ◽

2007 ◽

Vol 85 (11) ◽

pp. 1153-1159 ◽

Cited By ~ 16

Author(s):

Mahéra Al-Akoum ◽

Sylvie Dodin ◽

Ali Akoum

Keyword(s):

Breast Cancer ◽

Cell Proliferation ◽

Cell Growth ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Cytotoxic Effect ◽

Black Cohosh ◽

Proliferative Effect ◽

Dose Dependent ◽

Mcf 7

Breast cancer cell cultures were exposed to different concentrations of black cohosh, estradiol (E2), and tamoxifen to examine the effect on cell proliferation; cytotoxicity was assessed by using sulforhodamine B (SRB) dye solution. E2 (10−10–10−8 mol/L) markedly stimulated the proliferation of MCF-7 cells (p < 0.01). Tamoxifen stimulated MCF-7 cell proliferation at 10−6 mol/L and 10−5 mol/L (p < 0.005) but inhibited in a dose-dependent fashion the proliferative effect of E2 (p < 0.001). Black cohosh alone did not show any stimulatory effect, but exhibited a cytotoxic effect, which was significant at 103 μg/mL (p < 0.001). Adding black cohosh at 100–103 μg/mL to E2 at 10−9 mol/L also resulted in a dose-dependent inhibition of E2 proliferative effect. Interestingly, the combination of black cohosh (100–103 μg/mL) with increasing tamoxifen concentrations further inhibited MCF-7 cell growth. On MDA-MB-231 cells, neither E2 nor tamoxifen displayed any detectable effect. However, black cohosh inhibited MDA-MB-231 cell proliferation at 103 μg/mL (p < 0.05), and this inhibitory effect was enhanced by increasing tamoxifen concentrations. This study reveals a cytotoxic effect of black cohosh on both estrogen-sensitive and estrogen-insensitive breast cancer cells and a synergism with tamoxifen for inhibition of cancerous cell growth.

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The SMRT Coregulator Enhances Growth of Estrogen Receptor-α-Positive Breast Cancer Cells by Promotion of Cell Cycle Progression and Inhibition of Apoptosis

Endocrinology ◽

10.1210/en.2014-1002 ◽

2014 ◽

Vol 155 (9) ◽

pp. 3251-3261 ◽

Cited By ~ 10

Author(s):

Julia K. Blackmore ◽

Sudipan Karmakar ◽

Guowei Gu ◽

Vaishali Chaubal ◽

Liguo Wang ◽

...

Keyword(s):

Breast Cancer ◽

Cell Cycle ◽

Estrogen Receptor ◽

Cancer Cells ◽

Cancer Progression ◽

Breast Cancer Cells ◽

Estrogen Receptor Α ◽

Breast Cancer Progression ◽

Apoptotic Genes ◽

Mcf 7

Abstract The SMRT coregulator functions as a dual coactivator and corepressor for estrogen receptor-α (ERα) in a gene-specific manner, and in several studies its elevated expression correlates with poor outcome for breast cancer patients. A specific role of SMRT in breast cancer progression has not been elucidated, but SMRT knock-down limits estradiol-dependent growth of MCF-7 breast cancer cells. In this study, small-interfering RNA (siRNA) and short-hairpin RNA (shRNA) approaches were used to determine the effects of SMRT depletion on growth of ERα-positive MCF-7 and ZR-75–1 breast cancer cells, as well as the ERα-negative MDA-MB-231 breast cancer line. Depletion of SMRT inhibited growth of ERα-positive cells grown in monolayer but had no effect on growth of the ERα-negative cells. Reduced SMRT levels also negatively impacted the anchorage-independent growth of MCF-7 cells as assessed by soft agar colony formation assays. The observed growth inhibitions were due to a loss of estradiol-induced progression through the G1/S transition of the cell cycle and increased apoptosis in SMRT-depleted compared with control cells. Gene expression analyses indicated that SMRT inhibits apoptosis by a coordinated regulation of genes involved in apoptosis. Functioning as a dual coactivator for anti-apoptotic genes and corepressor for pro-apoptotic genes, SMRT can limit apoptosis. Together these data indicate that SMRT promotes breast cancer progression through multiple pathways leading to increased proliferation and decreased apoptosis.

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Influence of anchoring moieties on new benzimidazole-based Schiff base copper(ii) complexes towards estrogen dependent breast cancer cells

Dalton Transactions ◽

10.1039/d0dt03873c ◽

2021 ◽

Vol 50 (10) ◽

pp. 3701-3716

Author(s):

Anup Paul ◽

Priya Singh ◽

Maxim L. Kuznetsov ◽

Anirban Karmakar ◽

M. Fátima C. Guedes da Silva ◽

...

Keyword(s):

Breast Cancer ◽

Schiff Base ◽

Cancer Cells ◽

Cell Line ◽

Breast Cancer Cells ◽

Cytotoxic Effect ◽

Line P ◽

Mcf 7

Effects of triphenylphosphonium and triethylammonium linkers on new benzimidazole based Schiff base copper(ii) complexes are described. A triphenylphosphonium anchored compound exhibits a better cytotoxic effect than cisplatin on the MCF-7 cell line.

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Silver Nanoparticles Modulate the Epithelial-to-Mesenchymal Transition in Estrogen-Dependent Breast Cancer Cells In Vitro

International Journal of Molecular Sciences ◽

10.3390/ijms22179203 ◽

2021 ◽

Vol 22 (17) ◽

pp. 9203

Author(s):

Michał Rakowski ◽

Szymon Porębski ◽

Agnieszka Grzelak

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Mitochondrial Membrane ◽

Breast Cancer Cells ◽

Epithelial To Mesenchymal Transition ◽

Calcium Flux ◽

Oxygen Species ◽

Mesenchymal Transition ◽

Marker Proteins ◽

Mcf 7

Silver nanoparticles (AgNPs) are frequently detected in many convenience goods, such as cosmetics, that are applied directly to the skin. AgNPs accumulated in cells can modulate a wide range of molecular pathways, causing direct changes in cells. The aim of this study is to assess the capability of AgNPs to modulate the metastasis of breast cancer cells through the induction of epithelial-to-mesenchymal transition (EMT). The effect of the AgNPs on MCF-7 cells was investigated via the sulforhodamine B method, the wound healing test, generation of reactive oxygen species (ROS), the standard cytofluorimetric method of measuring the cell cycle, and the expression of EMT marker proteins and the MTA3 protein via Western blot. To fulfill the results, calcium flux and HDAC activity were measured. Additionally, mitochondrial membrane potential was measured to assess the direct impact of AgNPs on mitochondria. The results indicated that the MCF-7 cells are resistant to the cytotoxic effect of AgNPs and have higher mobility than the control cells. Treatment with AgNPs induced a generation of ROS; however, it did not affect the cell cycle but modulated the expression of EMT marker proteins and the MTA3 protein. Mitochondrial membrane potential and calcium flux were not altered; however, the AgNPs did modulate the total HDAC activity. The presented data support our hypothesis that AgNPs modulate the metastasis of MCF-7 cells through the EMT pathway. These results suggest that AgNPs, by inducing reactive oxygen species generation, alter the metabolism of breast cancer cells and trigger several pathways related to metastasis.

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Human drug efflux transporter ABCC5 confers acquired resistance to pemetrexed in breast cancer

Cancer Cell International ◽

10.1186/s12935-021-01842-x ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Jihui Chen ◽

Zhipeng Wang ◽

Shouhong Gao ◽

Kejin Wu ◽

Fang Bai ◽

...

Keyword(s):

Breast Cancer ◽

Cancer Cells ◽

Cell Line ◽

Abc Transporters ◽

Breast Cancer Cells ◽

Breast Cancer Treatment ◽

Control Group ◽

Mcf 7

Abstract Aim Pemetrexed, a new generation antifolate drug, has been approved for the treatment of locally advanced or metastatic breast cancer. However, factors affecting its efficacy and resistance have not been fully elucidated yet. ATP-binding cassette (ABC) transporters are predictors of prognosis as well as of adverse effects of several xenobiotics. This study was designed to explore whether ABC transporters affect pemetrexed resistance and can contribute to the optimization of breast cancer treatment regimen. Methods First, we measured the expression levels of ABC transporter family members in cell lines. Subsequently, we assessed the potential role of ABC transporters in conferring resistance to pemetrexed in primary breast cancer cells isolated from 34 breast cancer patients and the role of ABCC5 in mediating pemetrexed transport and apoptotic pathways in MCF-7 cells. Finally, the influence of ABCC5 expression on the therapeutic effect of pemetrexed was evaluated in an in vivo xenograft mouse model of breast cancer. Results The expression levels of ABCC2, ABCC4, ABCC5, and ABCG2 significantly increased in the pan-resistant cell line, and the ABCC5 level in the MCF-7-ADR cell line was 5.21 times higher than that in the control group. ABCC5 expression was inversely correlated with pemetrexed sensitivity (IC50, r = 0.741; p < 0.001) in breast cancer cells derived from 34 patients. Furthermore, we found that the expression level of ABCC5 influenced the efflux and cytotoxicity of pemetrexed in MCF-7 cells, with IC50 values of 0.06 and 0.20 μg/mL in ABCC5 knockout and over-expression cells, respectively. In the in vivo study, we observed that ABCC5 affected the sensitivity of pemetrexed in breast tumor-bearing mice, and the tumor volume was much larger in the ABCC5-overexpressing group than in the control group when compared with their own initial volumes (2.7-fold vs. 1.3-fold). Conclusions Our results indicated that ABCC5 expression was associated with pemetrexed resistance in vitro and in vivo, and it may serve as a target or biomarker for the optimization of pemetrexed regimen in breast cancer treatment.

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