scholarly journals Deciphering Differential Life Stage Radioinduced Reproductive Decline in Caenorhabditis elegans through Lipid Analysis

2021 ◽  
Vol 22 (19) ◽  
pp. 10277
Author(s):  
Elizabeth Dufourcq-Sekatcheff ◽  
Stephan Cuiné ◽  
Yonghua Li-Beisson ◽  
Loïc Quevarec ◽  
Myriam Richaud ◽  
...  
Keyword(s):  
Nuclear Energy ◽  
Energy Use ◽  
Lipid Analysis ◽  
Life Stage ◽  
Radiation Stress ◽  
C Elegans ◽  
Full Development ◽  
Molecular Determinants ◽  
Radiation Induced ◽  
Ionizing Radiations

Wildlife is chronically exposed to various sources of ionizing radiations, both environmental or anthropic, due to nuclear energy use, which can induce several defects in organisms. In invertebrates, reproduction, which directly impacts population dynamics, has been found to be the most radiosensitive endpoint. Understanding the underlying molecular pathways inducing this reproduction decrease can help in predicting the effects at larger scales (i.e., population). In this study, we used a life stage dependent approach in order to better understand the molecular determinants of reproduction decrease in the roundworm C. elegans. Worms were chronically exposed to 50 mGy·h−1 external gamma ionizing radiations throughout different developmental periods (namely embryogenesis, gametogenesis, and full development). Then, in addition to reproduction parameters, we performed a wide analysis of lipids (different class and fatty acid via FAMES), which are both important signaling molecules for reproduction and molecular targets of oxidative stress. Our results showed that reproductive defects are life stage dependent, that lipids are differently misregulated according to the considered exposure (e.g., upon embryogenesis and full development) and do not fully explain radiation induced reproductive defects. Finally, our results enable us to propose a conceptual model of lipid signaling after radiation stress in which both the soma and the germline participate.

Use of Nuclear Energy and Sustainable Development

2014 ◽  
Vol 1070-1072 ◽  
pp. 353-356
Author(s):  
Fang Chen ◽  
Qiang Yao
Keyword(s):  
Sustainable Development ◽  
Nuclear Power ◽  
Nuclear Energy ◽  
Energy Use ◽  
Clean Energy ◽  
Human Society ◽  
Plant Safety ◽  
The Status ◽  
Nuclear Power Plant Safety ◽  
Safety Operation

Nuclear energy as a clean energy, with the gradual depletion of traditional energy, is particularly important. In this paper, the status of the application of nuclear technology was reviewed. And problems during the use of nuclear energy were addressed, including environmental issue generated by radioactive mineral extraction, radwaste disposal, nuclear power plant safety operation. Although, there are still some problems in the process of nuclear energy use, undeniably nuclear energy is still the hope for the future of human society. Therefore, the technical and management improvement can achieve sustainable development of nuclear energy, in order to ensure the safety of human energy use and sustainability.

Transmutation and the Global Nuclear Energy Partnership

2006 ◽  
Vol 985 ◽  
Author(s):  
James Bresee
Keyword(s):  
Nuclear Power ◽  
Power Plants ◽  
Nuclear Energy ◽  
Energy Use ◽  
Fuel Cycle ◽  
Spent Fuel ◽  
Closed Fuel Cycle ◽  
State Of The Union ◽  
Foreign Countries ◽  
The U.S

AbstractIn the January 2006 State of the Union address, President Bush announced a new Advanced Energy Initiative, a significant part of which is the Global Nuclear Energy Initiative. Its details were described on February 6, 2006 by the U.S. Secretary of Energy. In summary, it has three parts: (1) a program to expand nuclear energy use domestically and in foreign countries to support economic growth while reducing the release of greenhouse gases such as carbon dioxide. (2) an expansion of the U.S. nuclear infrastructure that will lead to the recycling of spent fuel and a closed fuel cycle and, through transmutation, a reduction in the quantity and radiotoxicity of nuclear waste and its proliferation concerns, and (3) a partnership with other fuel cycle nations to support nuclear power in additional nations by providing small nuclear power plants and leased fuel with the provision that the resulting spent fuel would be returned by the lessee to the lessor. The final part would have the effect of stabilizing the number of fuel cycle countries with attendant non-proliferation value. Details will be given later in the paper.

Molecular cloning and life stage expression pattern of a new acetylcholinesterase gene from the plant-parasitic nematode Meloidogyne incognita

Nematology ◽  
2003 ◽  
Vol 5 (2) ◽  
pp. 213-217 ◽  
Author(s):  
Jean-Baptiste Laffaire ◽  
Marie-Noëlle Rosso ◽  
Stéphanie Jaubert ◽  
Pierre Abad
Keyword(s):  
Meloidogyne Incognita ◽  
Life Stage ◽  
Distinct Group ◽  
Catalytic Triad ◽  
Pcr Analysis ◽  
Parasitic Nematodes ◽  
Reading Frame ◽  
C Elegans ◽  
Second Stage ◽  
Before And After

AbstractA new cDNA, named Mi-ace-2, encoding an acetylcholinesterase, was isolated from Meloidogyne incognita. The full-length cDNA, carrying the trans-spliced SL1 leader sequence, was 2122 bp long with an open reading frame of 2058 bp. The predicted protein shared 32.3% identity with the previously identified MI ACHE and 49.1% identity with the C. elegans ACE-2. The conserved motifs involved in the catalytic triad, the choline binding site and 11 aromatic residues lining the catalytic gorge were present in the MI-ACE-2 deduced protein. RT-PCR analysis showed that Mi-ace-2 is transcribed in second stage juveniles before and after hatching, in females and in males. Phylogenetic analysis showed that MI-ACE-2 and ACE-2 were clustered in a distinct group closely related to the acetylcholinesterases secreted by animal-parasitic nematodes.

scholarly journals Molecular basis of synaptic vesicle cargo recognition by the endocytic sorting adaptor stonin 2

2007 ◽  
Vol 179 (7) ◽  
pp. 1497-1510 ◽  
Author(s):  
Nadja Jung ◽  
Martin Wienisch ◽  
Mingyu Gu ◽  
James B. Rand ◽  
Sebastian L. Müller ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Synaptic Vesicles ◽  
Molecular Basis ◽  
C Elegans ◽  
Evolutionarily Conserved ◽  
Molecular Determinants ◽  
Direct Association ◽  
Endocytic Sorting ◽  
Synaptotagmin 1 ◽  
Stonin 2

Synaptic transmission depends on clathrin-mediated recycling of synaptic vesicles (SVs). How select SV proteins are targeted for internalization has remained elusive. Stonins are evolutionarily conserved adaptors dedicated to endocytic sorting of the SV protein synaptotagmin. Our data identify the molecular determinants for recognition of synaptotagmin by stonin 2 or its Caenorhabditis elegans orthologue UNC-41B. The interaction involves the direct association of clusters of basic residues on the surface of the cytoplasmic domain of synaptotagmin 1 and a β strand within the μ–homology domain of stonin 2. Mutation of K783, Y784, and E785 to alanine within this stonin 2 β strand results in failure of the mutant stonin protein to associate with synaptotagmin, to accumulate at synapses, and to facilitate synaptotagmin internalization. Synaptotagmin-binding–defective UNC-41B is unable to rescue paralysis in C. elegans stonin mutant animals, suggesting that the mechanism of stonin-mediated SV cargo recognition is conserved from worms to mammals.

Well-to-Wheels Analysis of Energy Use and Greenhouse Gas Emissions of Hydrogen Produced with Nuclear Energy

2006 ◽  
Vol 155 (2) ◽  
pp. 192-207 ◽  
Author(s):  
Ye Wu ◽  
Michael Q. Wang ◽  
Anant D. Vyas ◽  
David C. Wade ◽  
Temitope A. Taiwo
Keyword(s):  
Greenhouse Gas Emissions ◽  
Greenhouse Gas ◽  
Nuclear Energy ◽  
Energy Use ◽  
Gas Emissions ◽  
Well To Wheels

scholarly journals About Modern Requirements for Seismic Resistance Design and Evaluation of Seismic Safety of Ukrainian NPPs

2017 ◽  
pp. 9-13
Author(s):  
D. Ryzhov ◽  
O-i. Shugaio ◽  
O-r. Shugaylo ◽  
O. Kendzera ◽  
M. Marienkov ◽  
...  
Keyword(s):  
Nuclear Energy ◽  
Energy Use ◽  
Radiation Safety ◽  
Seismic Resistance ◽  
Seismic Safety ◽  
Legal Documents ◽  
Nuclear And Radiation Safety ◽  
The Law ◽  
Operating Units

According to Articles 22 and 24 of the Law of Ukraine “On Nuclear Energy Use and Radiation Safety” and with the aim of improving the regulatory and legal documents regulating nuclear and radiation safety of nuclear installations, the regulation “Requirements for Seismic Resistance Design and Evaluation of Seismic Safety of Ukrainian NPPs: NP 306.2.208-2016” was developed. The paper presents modern requirements included into NP 306.2.208-2016. The emphasis was done on new requirements that were not previously available in PNAE G-006-87, but should be taken into account both in the design of new NPP units and in the assessment/reassessment of seismic resistance of operating units.

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