MMP-2 and MMP-9 Polymorphisms and Preeclampsia Risk in Tunisian Arabs: A Case-Control Study

The abnormal production of matrix metalloproteinases (MMPs), especially MMP-9 and MMP-2, plays a pivotal role in hypertensive disorders of pregnancy, and as such, can influence the development of preeclampsia. These alterations may result from functional genetic polymorphisms in the promoter region of MMP-9 and MMP-2 genes, which modify MMP-9 and MMP-2 expression. We investigated the association of MMP-9 polymorphism rs3918242 (-1562 C>T) and MMP-2 polymorphism rs2285053 (-735 C>T) with the risk of preeclampsia. This case–control study was conducted on 345 women with preeclampsia and 281 age-matched women with normal pregnancies from Tunisian hospitals. Genomic DNA was extracted from whole blood collected at delivery. Genotypes for -1562 C>T and -735 C>T polymorphisms were performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). An increased frequency of heterozygous MMP-9 -1562 C/T genotype carriers was observed in women with preeclampsia compared to healthy controls (p = 0.03). In contrast, the MMP-2 -735 C>T polymorphism was not significantly different regarding frequency distribution of the allele and genotype between healthy pregnant women and women with preeclampsia. Our study suggests that the MMP-9 -1562 C/T variant, associated with high MMP-9 production, could be a genetic risk factor for preeclampsia in Tunisian women.

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The association between IL-17 gene variants and risk of colorectal cancer in a Chinese population: A case–control study

Bioscience Reports ◽

10.1042/bsr20190013 ◽

2019 ◽

Vol 39 (11) ◽

Author(s):

Haiyang Feng ◽

Rongbiao Ying ◽

Tengjiao Chai ◽

Hailang Chen ◽

Haixing Ju

Keyword(s):

Colorectal Cancer ◽

Chinese Population ◽

Case Control Study ◽

Fragment Length Polymorphism ◽

Case Control ◽

Chain Reaction ◽

Node Metastasis ◽

Increased Risk ◽

Polymerase Chain ◽

Control Study

Abstract Interleukin (IL)-17 have been reported to be associated with the pathogenesis of colorectal cancer (CRC). Few studies investigated the association between IL-17 gene polymorphisms and risk of CRC with inconsistent findings. Thus, we recruited 352 CRC cases and 433 controls in a Chinese population and their genotyping was done using polymerase chain reaction-restriction fragment length polymorphism method. Our data showed that IL-17A rs2275913 polymorphism was associated with the increased risk of CRC, while no association was observed for IL-17F rs763780 polymorphism. Stratified analyses revealed that the significant association was also obtained in the females, smokers, drinkers and age ≥ 60 years groups for rs2275913 polymorphism. Moreover, the CC and/or GC genotype of rs2275913 polymorphism were correlated with TNM stage and lymph node metastasis. No association was shown between IL-17F rs763780 polymorphism and clinical characteristics of CRC. In conclusion, our data indicate that IL-17A rs2275913 polymorphism but not IL-17F rs763780 polymorphism contributes to increased risk for CRC patients in this Chinese population.

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ATG7 Polymorphisms rs7625184 and rs2606750 are Not Associated with Parkinson’s Disease: A Case Control Study

10.21203/rs.3.rs-257405/v1 ◽

2021 ◽

Author(s):

Ming Zou ◽

Jian-Yong Wang ◽

Ren-Jun Lv ◽

Shan-Jing Nie ◽

Lan-Bing Zhu ◽

...

Keyword(s):

Parkinson’S Disease ◽

Parkinson's Disease ◽

Case Control Study ◽

Fragment Length Polymorphism ◽

Case Control ◽

Nucleotide Polymorphisms ◽

Chain Reaction ◽

Single Nucleotide ◽

Polymerase Chain ◽

Control Study

Abstract Background: Deregulation of autophagy is involved in the development and progression of Parkinson’s disease. ATG7, an E1 like enzyme, palys a key role in autophagy. This study aimed to investigating the association between ATG7 polymorphisms and PD susceptibility. Methods: Single nucleotide polymorphisms of ATG7, including rs7625184 and rs2606750, were identified by polymerase chain reaction-restriction fragment length polymorphism in a Han Chinese population consisting of 312 PD patients and 309 healthy controls. Results: Genotyping analyses showed that none of the 2 SNPs was significantly associated with PD risk.Conclusions: Our results suggest that rs7625184 and rs2606750 are not associated with PD susceptibility. Further studies are warranted in revealing the links between ATG7 and PD.

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Analysis of glutathione S-transferase genes polymorphisms and the risk of schizophrenia in a sample of Iranian population

Neuron Glia Biology ◽

10.1017/s1740925x12000130 ◽

2011 ◽

Vol 7 (2-4) ◽

pp. 199-203 ◽

Cited By ~ 7

Author(s):

Farah Lotfi Kashani ◽

Dor Mohammad Kordi-Tamandani ◽

Roya Sahranavard ◽

Mohammad Hashemi ◽

Farzaneh Kordi-Tamandani ◽

...

Keyword(s):

Genomic Dna ◽

Case Control Study ◽

Gene Interaction ◽

Case Control ◽

Glutathione S Transferase ◽

Chain Reaction ◽

Healthy Control ◽

Glutathione S Transferases ◽

Polymerase Chain ◽

Control Study

Glutathione S-transferases (GSTs) are major intracellular antioxidants, which, impaired in their function, are involved in the progress of schizophrenia (SCZ). The aim of this case-control study was to investigate the association between the polymorphism of glutathione S-transferases M1 (GSTM1), T1 (GSTT1), the glutathione S-transferase P1 gene (GSTP1) and SCZ. We isolated genomic DNA from peripheral blood of 93 individuals with SCZ and 99 healthy control subjects' genotypes analyzing them for GSTM1, GSTT1 and GSTP1 using polymerase chain reaction. The analysis of the gene–gene interaction between GSTs indicated that the magnitude of the association was greater for the combined AG/GSTT1 & GSTM1 genotypes (OR = 2.51; 95% CI: 1.13–5.63, P = 0.02). The AG and combined AG + GG genotypes of GSTP1 increased the risk of SCZ (OR = 1.83; 95% CI: 0.94–3.75 and OR = 1.71; 95% CI: 0.92–3.19, respectively). The genotypes of GSTT/NULL, NULL/GSTM and NULL/NULL increased the risk of SCZ (OR = 2.05; 95% CI: 0.9–4.74; OR = 2.0; 95% CI: 1.68–2.31; and OR = 1.8; 95% CI: 0.57–2.46, respectively). The present study supports previous data that suggest that impairment in the function of GSTs genes may increase the risk of SCZ.

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The association between MMP-1 gene rs1799750 polymorphism and knee osteoarthritis risk

Bioscience Reports ◽

10.1042/bsr20181257 ◽

2018 ◽

Vol 38 (5) ◽

Cited By ~ 4

Author(s):

Rui Geng ◽

Yuansheng Xu ◽

Wenhao Hu ◽

Hui Zhao

Keyword(s):

Case Control Study ◽

Fragment Length Polymorphism ◽

Case Control ◽

Chinese Han ◽

Knee Oa ◽

Matrix Metalloproteinase 1 ◽

Standard Polymerase Chain Reaction ◽

Different Populations ◽

Polymerase Chain ◽

Control Study

Matrix metalloproteinase 1 (MMP-1) degrades cartilage, which may result in osteoarthritis (OA) development. Several studies have explored the association between MMP-1 gene rs1799750 polymorphism and OA in different populations. However, the results are inconsistent. The aim of this case–control study was to investigate the association between MMP-1 gene rs1799750 polymorphism and knee OA in a Chinese population. The present study included 308 cases and 404 controls. Genotyping was performed using standard polymerase chain reaction and restriction fragment length polymorphism. The present study found that 2G2G genotype (2G2G vs 1G1G: OR & 95% CI, 2.28 (1.47–3.53), P<0.001; 2G2G + 1G2G vs 1G1G: OR & 95% CI, 1.61 (1.15–2.24), P=0.005; 2G2G vs 1G2G + 1G1G: OR & 95% CI, 1.84 (1.26–2.68), P=0.002) or 2G allele carriers (2G vs 1G: OR & 95% CI, 1.48 (1.20–1.83), P<0.001) of MMP-1 gene rs1799750 polymorphism increased the risk of OA. In conclusion, this case–control study confirms that MMP-1 gene rs1799750 polymorphism increases the risk of knee OA in Chinese Han population.

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OPN gene locus is associated with the risk of knee osteoarthritis: a case–control study

Bioscience Reports ◽

10.1042/bsr20182023 ◽

2019 ◽

Vol 39 (2) ◽

Cited By ~ 2

Author(s):

Houlai Shang ◽

Yuedong Hao ◽

Wenhao Hu ◽

Xiaohui Hu ◽

Qing Jin

Keyword(s):

Knee Osteoarthritis ◽

Chinese Population ◽

Gene Locus ◽

Case Control Study ◽

Fragment Length Polymorphism ◽

Case Control ◽

Chinese Han ◽

Knee Oa ◽

Pcr Rflp ◽

Control Study

AbstractBackground/aims: Studies have demonstrated that osteopontin (OPN) was associated with the severity and development of knee osteoarthritis (OA). Methods: The purpose of this case–control study was to investigate the association between OPN gene rs11730582 polymorphism and knee OA risk in a Chinese population. Genotyping was analyzed using standard PCR and restriction fragment length polymorphism (PCR-RFLP). Results: The present study found that C allele or CC genotype of OPN gene rs11730582 polymorphism was related to decreased risk for knee OA. Furthermore, positive associations were obtained amongst the females, and body mass index (BMI) < 25 kg/m2 groups. Conclusions: To sum up, the present study reveals that OPN gene rs11730582 polymorphism decreases the risk of knee OA in Chinese Han population.

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A Case-control Study on the Association of Mitochondrial Transcription Factor a Gene +35G/C Polymorphism and Mitochondrial DNA Copy Number with the Risk of Endometriosis in Indian Women

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2021/v30i730279 ◽

2021 ◽

pp. 60-67

Author(s):

Himabindu Beeram ◽

Tumu Venkat Reddy ◽

Suresh Govatati ◽

Swapna Siddamalla ◽

Mamata Deenadayal ◽

...

Keyword(s):

Copy Number ◽

Case Control Study ◽

Case Control ◽

Mtdna Copy Number ◽

Chain Reaction ◽

Indian Women ◽

Mitochondrial Transcription Factor ◽

Mitochondrial Transcription Factor A ◽

Polymerase Chain ◽

Control Study

Aim: The Mitochondrial transcription factor A (TFAM) and mitochondrial (mt) DNA copy number variations are known to contribute in disease development. Genetic factors play an important role in the development of endometriosis. Therefore, this case–control study aimed to analyze the association of TFAM+35G/C polymorphism and mitochondrial copy number with the risk of endometriosis in Indian women. Study Design: This study was carried out on 418 subjects including 200 endometriosis cases and 218 controls. Methodology: Genotyping of TFAM +35G/C polymorphism (rs1937) was carried out by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Quantification of mtDNA copy number was carried out using a real time quantitative polymerase chain reaction (qRT-PCR). Place and Duration of Study: Department of Biochemistry, Osmania University, 2014 to 2020. Results: TFAM genotype as well as allele distributions were all in Hardy-Weinberg equilibrium. The results indicated a significant reduction of GG genotype frequency (P=0.009), high ‘C’ allele frequency (P=0.017) and significantly decreased mtDNA copy number in endometriosis cases compared to controls (P= 0.0001). Conclusion: Present study revealed a statistically significant association of decreased GG genotype of TFAM +35G/C polymorphism and mtDNA copy number with the risk of developing endometriosis in Indian women.

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Acute‐onset smell and taste disorders in the context of COVID‐19: a pilot multicentre polymerase chain reaction based case–control study

European Journal of Neurology ◽

10.1111/ene.14273 ◽

2020 ◽

Vol 27 (9) ◽

pp. 1738-1741 ◽

Cited By ~ 65

Author(s):

Á. Beltrán‐Corbellini ◽

J. L. Chico‐García ◽

J. Martínez‐Poles ◽

F. Rodríguez‐Jorge ◽

E. Natera‐Villalba ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Case Control Study ◽

Case Control ◽

Acute Onset ◽

Chain Reaction ◽

Taste Disorders ◽

Polymerase Chain ◽

Control Study

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Frequency of HLA-DRB1 Gene Alleles in Patients With Multiple Sclerosis in a Lithuanian Population

Medicina ◽

10.3390/medicina48010002 ◽

2012 ◽

Vol 48 (1) ◽

pp. 2

Author(s):

Renata Balnytė ◽

Daiva Rastenytė ◽

Dalia Mickevičienė ◽

Antanas Vaitkus ◽

Erika Skrodenienė ◽

...

Keyword(s):

Multiple Sclerosis ◽

Case Control Study ◽

Case Control ◽

Chain Reaction ◽

Female Patients ◽

History Of ◽

Male Patients ◽

Polymerase Chain ◽

The Relationship ◽

Control Study

The aim of the present study was to investigate the influence of HLA-DRB1 alleles on the genetic susceptibility to multiple sclerosis in the Lithuanian population. Material and Methods. A total of 120 patients with multiple sclerosis and 120 unrelated healthy controls were enrolled in this case-control study. Allelic frequencies were compared between the groups. HLA-DRB1 alleles were genotyped using the polymerase chain reaction. Results. HLA-DRB1*15 was present in 55.8% of the patients with multiple sclerosis and 10.0% of the controls (OR, 5.58; 95% CI, 3.19–9.77; P<0.0001). The protective alleles that were found to be more prevalent among the controls compared with the patients with multiple sclerosis were HLADRB1* 01 (26.7% vs. 7.5%, P<0.0001), *03 (17.5% vs. 8.3%, P=0.034), and *16 (11.7% vs. 3.3%, P=0.014). HLA-DRB1*15 was more common among the female patients with multiple sclerosis than among the male patients (68.4% vs. 34.1%; OR, 4.18; 95%, CI 1.90–9.22; P=0.001). The heterozygous inheritance of HLA-DRB1*15 allele was more common in the patients with a history of maternal multiple sclerosis than in those with a history of paternal multiple sclerosis (29.4% vs. 9.8%; P=0.045). Conclusions. HLA-DRB1*15 was found to be associated with multiple sclerosis in the Lithuanian population. This allele was more prevalent among the female patients with multiple sclerosis. Maternal multiple sclerosis was more common than paternal multiple sclerosis, but the relationship with HLA-DRB1*15 allele was not established. HLA-DRB1*01, *03, and *16 appeared to be the protective alleles in this series.

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