scholarly journals Prevalence of Current and Past SARS-CoV-2 Infections among Police Employees in Poland, June–July 2020

2020 ◽  
Vol 9 (10) ◽  
pp. 3245 ◽  
Author(s):  
Mariusz Gujski ◽  
Mateusz Jankowski ◽  
Jarosław Pinkas ◽  
Waldemar Wierzba ◽  
Piotr Samel-Kowalik ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Police Officers ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Cross Sectional Survey ◽  
Serological Tests ◽  
Cross Sectional ◽  
Igg Index ◽  
Civilian Employees ◽  
June July

Background: Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We aimed to determine the prevalence of current and past SARS-CoV-2 infections among police employees. Methods: This cross-sectional survey was undertaken among 5082 police employees from Mazowieckie Province, Poland. RT-PCR testing for current SARS-CoV-2 infection and serological tests (ELISA) for the presence of anti-SARS-CoV-2 IgM+IgA and IgG antibodies were performed. Results: All RT-PCR tests were negative. The anti-SARS-CoV-2 IgM+IgA index was positive (>8) in 8.9% of participants, including 11.2% women and 7.7% men (p < 0.001). Equivocal IgM+IgA index (6–8) was found in 9.8% of participants, including 11.9% women and 8.7% men (p < 0.001). The IgG index was positive (>6) in 4.3% and equivocal (4–6) in 13.2% of participants. A higher odds of positive IgM+IgA index was found in women vs. men (OR: 1.742) and police officers vs. civilian employees (OR: 1.411). Participants aged ≥60 years had a higher odds of positive IgG index vs. those aged 20–29 years (OR: 3.309). Daily vaping also increased the odds of positive IgG index (OR: 2.058). Conclusions: The majority of Polish police employees are seronegative for SARS-CoV-2 infection. Vaping and older age (≥60 years) were associated with a higher risk of SARS-CoV-2 infection.

scholarly journals Evaluation of nine serological rapid tests for the detection of SARS-CoV-2

2020 ◽  
Vol 44 ◽  
pp. 1
Author(s):  
Marcela Mercado ◽  
Jeadran Malagón-Rojas ◽  
Gabriela Delgado ◽  
Vivian Vanesa Rubio ◽  
Lida Muñoz Galindo ◽  
...  
Keyword(s):  
Study Groups ◽  
Cross Sectional Study ◽  
Igg Antibodies ◽  
Sectional Study ◽  
Rt Pcr ◽  
Serum Samples ◽  
Serological Tests ◽  
Cross Sectional ◽  
Diagnostic Aid ◽  
Rapid Tests

Objective. To evaluate the operative capacity of nine serological rapid tests to detect the IgM/IgG antibodies response in serum from patients with SARS-CoV-2 in different clinical stages. Methods. A cross-sectional study of serological rapid tests was designed to compare the performance of the evaluated immunochromatographic tests for the diagnosis of SARS-CoV-2. A total of 293 samples was used, including negatives, asymptomatic, and symptomatic serum samples. Results. The sensitivity of the evaluated tests was low and moderate in the groups of asymptomatic serum samples and the group of serums coming from patients with less than 11 days since the onset of the symptoms. The specificity for the anti-SARS-CoV-2 antibodies tests ranged between 86.5%-99% for IgM and 86.5%-99.5% for IgG. The sensitivity and the likelihood ratio were different according to the study groups. The usefulness of these tests is restricted to symptomatic patients and their sensitivity is greater than 85% after 11 days from the appearance of symptoms. Conclusions. Serological tests are not an adequate strategy for the identification of asymptomatic and pre-symptomatic patients. Serological rapid tests for the detection of specific anti-SARS-CoV-2 antibodies can be used as a diagnostic aid, but diagnosis must be confirmed by RT-PCR. Rapid tests should be reserved for patients with symptoms lasting more than 11 days.

Examining police officers’ perceptions of stress: the role of Person−environment fit

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Rachael Rief ◽  
Samantha Clinkinbeard
Keyword(s):  
Job Satisfaction ◽  
Police Officers ◽  
Well Being ◽  
Work Outcomes ◽  
Cross Sectional Survey ◽  
Organizational Fit ◽  
Cross Sectional ◽  
Content Type ◽  
The Relationship ◽  
Overall Job Satisfaction

PurposeThe purpose of the study was to examine the relationship between officer perceptions of fit in their organization and stress (organizational and operational), overall job satisfaction and turnover contemplation (within the last 6 months).Design/methodology/approachThe authors used cross-sectional survey data from a sample of 832 officers from two Midwest police departments to examine the relationships between fit, stress and work-related attitudes.FindingsPerceived stress and organizational fit were strong predictors of overall job satisfaction and turnover contemplation; organizational fit accounted for the most variation in stress, satisfaction and turnover contemplation. Organizational stress partially mediated the relationship between organizational fit and job satisfaction and organizational fit and turnover contemplation.Research ImplicationsMore research is needed to identify predictors of organizational fit perceptions among police officers.Practical implicationsFindings indicate that agencies should pay close attention to the organizational culture and structure when trying to address issues of officer well-being and retention. Further, the person−environment framework can be a useful tool in examining police occupational outcomes.Originality/valueThe authors findings contribute to research on officer stress by exploring perceptions of organizational fit as a predictor of stress and unpacking how officer stress matters to important work outcomes, including job satisfaction and thoughts of turnover, by considering stress as a mediator between organizational fit and these work outcomes.

scholarly journals Use of online cultural content for mental health and well-being during COVID-19 restrictions: cross-sectional survey

2021 ◽  
pp. 1-10
Author(s):  
Rebecca J. Syed Sheriff ◽  
Helen Adams ◽  
Evgenia Riga ◽  
Andrew K. Przybylski ◽  
Laura Bonsaver ◽  
...  
Keyword(s):  
Mental Health ◽  
Well Being ◽  
Vulnerable Groups ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Online Culture ◽  
Reported Data ◽  
June July ◽  
The Uk ◽  
Health And Well Being

Aims and method To gain a deeper understanding of the use of online culture and its potential benefits to mental health and well-being, sociodemographic characteristics and self-reported data on usage, perceived mental health benefits and health status were collected in an online cross-sectional survey during COVID-19 restrictions in the UK in June–July 2020. Results In total, 1056 people completed the survey. A high proportion of participants reported finding online culture helpful for mental health; all but one of the benefits were associated with regular use and some with age. Reported benefits were wide-ranging and interconnected. Those aged under 25 years were less likely to be regular users of online culture or to have increased their use during lockdown. Clinical implications There may be benefits in targeting cultural resources for mental health to vulnerable groups such as young adults.

scholarly journals The Association Between Stress Mindset and Physical and Psychological Wellbeing: Testing a Stress Beliefs Model in Police Officers

2020 ◽  
Author(s):  
Jacob J Keech ◽  
Kaitlyn L. Cole ◽  
Martin S Hagger ◽  
Kyra Hamilton
Keyword(s):  
Perceived Stress ◽  
Police Officers ◽  
Somatic Symptoms ◽  
Proactive Coping ◽  
Psychological Wellbeing ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Effects Of Stress ◽  
Emergency Service Workers ◽  
Coping Behaviours

Objective: Emergency service workers like police officers experience high levels of stress in the course of their regular duties. Holding particular stress mindsets may help to mitigate the deleterious effects of stress and promote wellbeing in workers experiencing regular stress. The study aimed to examine the processes by which stress mindsets relate to health and wellbeing in police officers. A stress beliefs model in which perceived somatic symptoms and coping behaviours mediate effects of stress mindsets on outcomes was tested. Design: Police officers (N=134) completed an online cross-sectional survey. Main outcome measures: Perceived somatic symptoms, proactive coping behaviours, physical and psychological wellbeing, and perceived stress. Results: Bayesian path analysis with informative priors revealed indirect effects of stress mindsets on psychological wellbeing and perceived stress through proactive coping behaviours and perceived somatic symptoms. Physical and psychological wellbeing, and perceived stress were predicted by stress mindsets directly, and through perceived somatic symptoms. Conclusion: The findings support model predictions that behaviours aimed at proactively meeting demands and perceived somatic symptoms mediated the relationship between stress mindset and health-related outcomes. The findings provide further foundational knowledge on mechanisms through which stress mindset is associated with outcomes and can inform future longitudinal and experimental research.

scholarly journals Social Distancing to Mitigate COVID-19 Risks Is Associated With COVID-19 Discriminatory Attitudes Among People Living with HIV

2020 ◽  
Vol 54 (10) ◽  
pp. 728-737
Author(s):  
Marcie Berman ◽  
Lisa A Eaton ◽  
Ryan J Watson ◽  
J L Andrepont ◽  
Seth Kalichman
Keyword(s):  
Social Sciences ◽  
Public Health ◽  
Severe Acute Respiratory Syndrome ◽  
Ongoing Study ◽  
People Living With Hiv ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Immune Systems ◽  
Social Distancing ◽  
Living With Hiv

Abstract Background Severe acute respiratory syndrome coronavirus-2, the virus that causes COVID-19, is an emerging pandemic with heightened concerns for people with compromised immune systems, including people living with HIV. Purpose In the absence of a vaccine, public health messaging to mitigate risks for COVID-19 primarily focuses on social distancing. Because people living with HIV commonly experience mistreatment associated with HIV, their response to social distancing may be complicated by psychosocial attitudes associated with COVID-19. Methods To evaluate these relationships, we conducted a rapid-response, cross-sectional survey with people living with HIV (N = 149) to assess social distancing practices, COVID-19 discriminatory attitudes, COVID-19 xenophobic attitudes, HIV microaggressions, and concern over contracting COVID-19. Data were collected from participants enrolled in a larger ongoing study between March 30, 2020 and April 17, 2020. Results Results indicated that choosing to socially distance to reduce COVID-19 exposure was associated with COVID-19 discriminatory attitudes, concerns of contracting COVID-19, and identifying as transgender. Likewise, social distancing imposed by others (e.g., cancelations and restrictions) was associated with concerns of contracting COVID-19. Conclusions Findings demonstrate that social distancing measures are related to concerns of contracting the virus and discriminatory attitudes toward those who are presumed to be living with COVID-19. These potentially negative psychosocial attitudes toward people perceived to have COVID-19 echo the discriminatory actions and attitudes that we continue to observe in HIV social sciences research.

scholarly journals Performance of Commercially Available Rapid Serological Assays for the Detection of SARS-CoV-2 Antibodies

Pathogens ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1067
Author(s):  
Anwar M. Hashem ◽  
Rowa Y. Alhabbab ◽  
Abdullah Algaissi ◽  
Mohamed A. Alfaleh ◽  
Sharif Hala ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Significant Variation ◽  
High Specificity ◽  
Lateral Flow ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Standard Methods ◽  
Specific Antibodies ◽  
Time Points

The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), continues to spread globally. Although several rapid commercial serological assays have been developed, little is known about their performance and accuracy in detecting SARS-CoV-2-specific antibodies in COVID-19 patient samples. Here, we have evaluated the performance of seven commercially available rapid lateral flow immunoassays (LFIA) obtained from different manufacturers, and compared them to in-house developed and validated ELISA assays for the detection of SARS-CoV-2-specific IgM and IgG antibodies in RT-PCR-confirmed COVID-19 patients. While all evaluated LFIA assays showed high specificity, our data showed a significant variation in sensitivity of these assays, which ranged from 0% to 54% for samples collected early during infection (3–7 days post symptoms onset) and from 54% to 88% for samples collected at later time points during infection (8–27 days post symptoms onset). Therefore, we recommend prior evaluation and validation of these assays before being routinely used to detect IgM and IgG in COVID-19 patients. Moreover, our findings suggest the use of LFIA assays in combination with other standard methods, and not as an alternative.

scholarly journals Antibody response and viraemia during the course of severe acute respiratory syndrome (SARS)-associated coronavirus infection

2004 ◽  
Vol 53 (5) ◽  
pp. 435-438 ◽  
Author(s):  
Weijun Chen ◽  
Zuyuan Xu ◽  
Jingsong Mu ◽  
Ling Yang ◽  
Haixue Gan ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Antibody Response ◽  
Time Course ◽  
Recovery Phase ◽  
Antibody Responses ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Blood Samples ◽  
Convalescent Phase ◽  
Coronavirus Infection

To understand the time-course of viraemia and antibody responses to severe acute respiratory syndrome-associated coronavirus (SARS-CoV), RT-PCR and ELISA were used to assay 376 blood samples from 135 SARS patients at various stages of the illness, including samples from patients who were in their early convalescent phase. The results showed that IgM antibodies decreased and became undetectable 11 weeks into the recovery phase. IgG antibodies, however, remained detectable for a period beyond 11 weeks and were found in 100 % of patients in the early convalescent phase. SARS-CoV viraemia mainly appeared 1 week after the onset of illness and then decreased over a period of 1 month, becoming undetectable in the blood samples of the convalescent patients. At the peak of viraemia, viral RNA was detectable in 75 % of blood samples from patients who were clinically diagnosed with SARS 1 or 2 weeks before the test.

scholarly journals Distribution and genetic diversity of Enterovirus G (EV-G) on pig farms in Thailand

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Taveesak Janetanakit ◽  
Supassama Chaiyawong ◽  
Kamonpan Charoenkul ◽  
Ratanaporn Tangwangvivat ◽  
Ekkapat Chamsai ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Characterization ◽  
Rt Pcr ◽  
Cross Sectional Survey ◽  
High Genetic Diversity ◽  
Cross Sectional ◽  
Predominant Genotype ◽  
Diarrhea Virus ◽  
Pig Farms

Abstract Background Enterovirus G (EV-G) causes subclinical infections and is occasionally associated with diarrhea in pigs. In this study, we conducted a cross-sectional survey of EV-G in pigs from 73 pig farms in 20 provinces of Thailand from December 2014 to January 2018. Results Our results showed a high occurrence of EV-Gs which 71.6 % of fecal and intestinal samples (556/777) and 71.2 % of pig farms (52/73) were positive for EV-G by RT-PCR specific to the 5’UTR. EV-Gs could be detected in all age pig groups, and the percentage positivity was highest in the fattening group (89.7 %), followed by the nursery group (89.4 %). To characterize the viruses, 34 EV-G representatives were characterized by VP1 gene sequencing. Pairwise sequence comparison and phylogenetic analysis showed that Thai-EV-Gs belonged to the EV-G1, EV-G3, EV-G4, EV-G8, EV-G9 and EV-G10 genotypes, among which the EV-G3 was the predominant genotype in Thailand. Co-infection with different EV-G genotypes or with EV-Gs and porcine epidemic diarrhea virus (PEDV) or porcine deltacoronavirus (PDCoV) on the same pig farms was observed. Conclusions Our results confirmed that EV-G infection is endemic in Thailand, with a high genetic diversity of different genotypes. This study constitutes the first report of the genetic characterization of EV-GS in pigs in Thailand.

scholarly journals Quantification of IgG specific antibodies in recovered COVID -19 patients and its association with disease severity

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Saurabh Srivastava ◽  
Shalini Bahadur ◽  
Shalini Shukla ◽  
Rashmi Upadhyay ◽  
Jaganathan Sickan ◽  
...  
Keyword(s):  
Vaccination Strategy ◽  
Public Health Research ◽  
Quantitative Assay ◽  
Serological Response ◽  
Igg Antibodies ◽  
Rt Pcr ◽  
Serological Tests ◽  
Entire Cohort ◽  
Mild Disease ◽  
The Mean

Background: Serological tests for anti-SARS-CoV-2 antibodies can provide valuable information in epidemiologic and public health research. However, the data is limited on serological response post COVID-19 infection. Objective: To investigate the use of ARCHITECT SARS CoV-2 IgG II Quantitative assay to quantify the IgG antibodies in recovered COVID-19 infections. Methods: Abbott SARS-CoV-2 IgG assays were performed using the ARCHITECT Quantitative chemiluminescent microparticle immunoassay to detect IgG against SARS-CoV-2 S1 receptor binding domain (RBD) in serum and plasma. Patients having IgG concentrations above ≥50 AU/mL were defined as positive. We analyzed 333 COVID-19 patients who were tested negative by RT-PCR not more than three months after testing positive. Results: The mean age of the population was 34.3 years and 95% were male. About 93% of the patients had mild disease and rest had moderate disease. The mean IgG levels in the entire cohort was 1860.27 AU/mL, the lowest and highest value detected by ARCHITECT SARS CoV-2 IgG II Quantitative Assay (Abbott Laboratories, USA) were 39.8 AU/mL and 43657.4 AU/mL, respectively. While patients with moderate COVID-19 disease had numerically higher IgG levels than patients with mild disease, the difference was not statistically significant (1787.7 AU/mL vs. 2865.39 AU/mL, p=0.21). In 97% of the patients, IgG antibodies could be detected up to 95 days after testing negative on RT-PCR. Conclusion: Patients with moderate COVID-19 disease may develop higher IgG antibodies than in patients with mild disease. Antibodies can be detected in majority of recovered individuals for more than 90 days which may be valuable in developing future vaccination strategy.

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