scholarly journals Temporal Patterns and Inter-Correlations among Physical and Antioxidant Attributes and Enzyme Activities of Apricot Fruit Inoculated with Monilinia laxa under Salicylic Acid and Methyl Jasmonate Treatments under Shelf-Life Conditions

2021 ◽  
Vol 7 (5) ◽  
pp. 341
Author(s):  
Ahmed Ezzat ◽  
Szilárd Szabó ◽  
Zoltán Szabó ◽  
Attila Hegedűs ◽  
Dorina Berényi ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Plant Defense ◽  
Shelf Life ◽  
Methyl Jasmonate ◽  
Enzyme Activities ◽  
Brown Rot ◽  
Temporal Patterns ◽  
Storage Conditions ◽  
Total Variance ◽  
Monilinia Laxa

Monilinia laxa causes serious postharvest damage on apricot fruits under shelf-life storage conditions. Plant elicitors of methyl jasmonate (MeJA) and salicylic acid (SA) can reduce this damage, and their research can explain the background of the plant defense physiological processes in M. laxa-infected fruits. The aims of this study were: (i) to evaluate the effect of various concentrations of MeJA and SA on brown rot incidence (BRI) and lesion diameter (LD) of apricot fruits; (ii) to measure the temporal patterns for the effect of 0.4 mmol L−1 MeJA and 2 mmol L−1 SA treatments on BRI, LD and seven fruit measures (fruit firmness (FF), lignin content (LC), total soluble phenol content (TSPC), total antioxidant capacity (TAC) and enzyme activities of PAL, POD and SOD) in treatments of M. laxa-inoculated versus (vs.) non-inoculated fruits over an eight-day shelf-life storage period; and (iii) to determine inter-correlations among the seven fruit measures for MeJA and SA treatments. Both MeJA and SA significantly reduced BRI and LD. LC, FF, TAC, TSPC, as well as SOD and PAL activities in the MeJA and SA treatments were higher than the water-treated control in most assessment days and both inoculation treatments. In both inoculation treatments, the activity of POD in the SA-treated fruits was higher than MeJA-treated and control fruits at all dates. In MeJA vs. SA and inoculated vs. non-inoculated treatments, six variable pairs (FF vs. TSPC, FF vs. TAC, TAC vs. PAL, PAL vs. POD, PAL vs. SOD, and POD vs. SOD) showed significant inter-correlation values. Principal component analyses explained 96% and 93% of the total variance for inoculated and non-inoculated treatments, respectively. In inoculated treatments, both PC1 and PC2 explained 41% of the total variance and correlated with FF, TSPC and TAC and with PAL, SOD and POD, respectively. In non-inoculated treatments, PC1 and PC2 explained 49% and 44% of the total variance and correlated with LC, PAL, POD and SOD and with FF, TSPC and TAC, respectively. It can be concluded that MeJA and SA are useful in the practice to enhance the plant defense system against brown rot by reducing fungal growth and by improving physical and antioxidant attributes (FF, LC, TAC and TSPC) and the activity of defense-related enzymes (PAL, POD and SOD) in apricot fruits during shelf-life storage conditions.

scholarly journals Temporal Changes and Correlations between Quality Loss Parameters, Antioxidant Properties and Enzyme Activities in Apricot Fruit Treated with Methyl Jasmonate and Salicylic Acid during Cold Storage and Shelf-Life

2020 ◽  
Vol 10 (22) ◽  
pp. 8071
Author(s):  
Ahmed Ezzat ◽  
Attila Hegedűs ◽  
Szilárd Szabó ◽  
Amin Ammar ◽  
Zoltán Szabó ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Shelf Life ◽  
Methyl Jasmonate ◽  
Cold Storage ◽  
Enzyme Activities ◽  
Pearson Correlation ◽  
Antioxidant Properties ◽  
Storage Conditions ◽  
Temporal Changes ◽  
Quality Loss

The apricot storability is one of the largest challenges, which the apricot industry has to face all over the world; therefore, finding options for prolonging fruit quality during cold storage (CS) and shelf-life (SL) will help to decrease postharvest losses of apricot. The aim of this apricot fruit work was to study the temporal changes and correlations of 10 quality parameters (quality losses, antioxidant properties and enzyme activities) in the postharvest treatments of methyl jasmonate (MeJA) and salicylic acid (SA) under 1 °C CS (7, 14 and 21 days) and 25 °C SL (4 and 8 days after the 21-day CS) treatments. MeJA and SA significantly decreased the quality loss of chilling injury (CI) and fruit decay (FD) at all dates for both storage conditions. MeJA- and SA-treated fruits increased total antioxidant capacity (TAC), total soluble phenolic compounds (TSPC) and carotenoids contents (TCC) at all dates of both storage treatments. In contrast, the ascorbic acid content (AAC) increased only until days 14 and 4 in the CS and SL treatments, respectively. Among enzyme activity parameters, the activities of phenylalanine ammonia-lyase (PAL), peroxidase and superoxide dismutase (SOD) were significantly increased in the MeJA and SA treatments in all dates of both storage treatments. Catalase (CAT) activity increased in the SA and control treatments, while it decreased in the MeJA treatment in both storage conditions. In both the MeJA and the SA treatments, six pair-variables (FD vs. CI, PAL vs. CAT, PAL vs. SOD, TAC vs. SOD, TAC vs. FD, and AAC vs. CI) were significant in Pearson correlation and regression analyses among the 45 parameters pairs. Principal component analyses explained 89.3% of the total variance and PC1 accounted for 55.6% of the variance and correlated with the CI, FD, TAC, TSPC, TCC, PAL and SOD, indicating strong connections among most parameters. In conclusion, MeJA and SA are practically useful and inexpensive techniques to maintain quality attributes of CI, FD, TAC, TSPC, TCC, PAL, POD and SOD in apricot fruit during both CS and SL conditions.

scholarly journals Preliminary results on salicylic acid treatment on brown rot caused by Monilinia laxa on Jumbo Cot fruit, Prunus armeniaca L.

2013 ◽  
Vol 19 (3-4) ◽  
Author(s):  
A. Ezzat ◽  
Z. Szabó ◽  
J. Nyéki ◽  
I. J. Holb
Keyword(s):  
Salicylic Acid ◽  
Induced Resistance ◽  
Acid Treatment ◽  
Mycelial Growth ◽  
Brown Rot ◽  
Prunus Armeniaca ◽  
Control Treatment ◽  
Monilinia Laxa ◽  
Salicylic Acid Treatment ◽  
Prunus Armeniaca L

The effect of salicylic acid on reducing brown rot caused by Monilinia laxa infection in postharvest apricot fruit Prunus armeniaca L. Freshly harvest Jumpo Cot fruit were treated with water as control treatment and other group treated with salicylic acid 2 Mm as induced resistance treatment, Fruits were infected by M. laxa (1 × 10-3spores ml-1) and incubated at 25 oC for 2, 4 and 6 days. Treatment fruits with salicylic acid resulted in direct effect on mycelial growth as in the salicylic acid treatment the growth reached to 45% after 6 days of incubation while in the control treatment it reached to 100%.

scholarly journals Interaction of Monoterpenoids, Methyl Jasmonate, and Ca2+ in Controlling Postharvest Brown Rot of Sweet Cherry

HortScience ◽  
2000 ◽  
Vol 35 (7) ◽  
pp. 1304-1307 ◽  
Author(s):  
Rong Tsao ◽  
Ting Zhou
Keyword(s):  
Plant Defense ◽  
Methyl Jasmonate ◽  
Defense Mechanisms ◽  
Sweet Cherry ◽  
Prunus Avium ◽  
Control Strategies ◽  
Brown Rot ◽  
Monilinia Fructicola ◽  
Alternative Control ◽  
Acetate Methyl

The banning of synthetic fungicides for postharvest use on fruits in Canada has prompted a search for alternative control strategies for postharvest brown rot caused by Monilinia fructicola (Wint.) Honey on sweet cherry (Prunus avium L.). Thymol and carvacrol were the two most potent fungicides among the monoterpenoids tested. The brown rot incidences of M. fructicola-inoculated cherry dipped in 1000 μg·mL-1 thymol and carvacrol were 24% and 23%, respectively, compared with 81% for the control. The effects of thymol and carvacrol were not significantly enhanced by the addition of CaCl2 or CaB'y®, a foliar calcium fertilizer. Decco® 282 significantly reduced the activity of thymol. Methyl jasmonate, an elicitor of plant defense mechanisms, did not reduce brown rot by itself, and did not increase the efficacy of thymol and carvacrol when used as an additive in dipping or fumigation experiments. Thymol and carvacrol caused stem browning of cherry fruits in the fumigation experiment, however, 69% and 73%, respectively, of the browning was prevented when methyl jasmonate was used as a co-fumigant. Chemical names used: 5-methyl-2-(1-methylethyl)phenol (thymol); 2-methyl-5-(1-methylethyl)phenol (carvacrol); methyl 3-oxo-2-(2-pentenyl)cyclopentane acetate (methyl jasmonate).

scholarly journals On the Biochemical and Physiological Responses of ‘Crimson Seedless’ Grapes Coated with an Edible Composite of Pectin, Polyphenylene Alcohol, and Salicylic Acid

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 498
Author(s):  
A. A. Lo’ay ◽  
M. M. Rabie ◽  
Haifa A. S. Alhaithloul ◽  
Suliman M. S. Alghanem ◽  
Aly M. Ibrahim ◽  
...  
Keyword(s):  
Weight Loss ◽  
Cell Wall ◽  
Salicylic Acid ◽  
Shelf Life ◽  
Enzyme Activities ◽  
Soluble Solid Content ◽  
H2o2 Production ◽  
Storage Duration ◽  
Cell Wall Damage ◽  
Seedless Grape

The ‘Crimson seedless’ grape encountered several difficulties during shelf life, including weight loss, rachis browning, and berry shattering. The effect of exogenous pectin (PE) and polyphenol alcohol (PVA) with supporting salicylic acid (SA) at different concentrations (0, 1, and 2 mM) was applied. The coating was applied to bunches for 5 min and stored at room temperature (26 ± 1 °C and RH 65 ± 3%) for 4 days. In this study, postharvest application of PE + PVA-SA can significantly reduce the cell wall degradation enzyme activities of ‘Crimson seedless’ grape during shelf life. ‘Crimson seedless’ bunches, treated with PE + PVA-SA 2 mmol L−1, had a lowered rachis browning index (RB index), weight loss (WL%), and berry shattering percentage (BS%) and preserved berry color hue angle (ho) compared to untreated bunches during shelf-life duration. Moreover, the PE + PVA-SA 2 mM improved berry firmness (BF) and removal force (BRF). It also improved the soluble solid content (SSC%), titratable acidity (TA%), and SSC: TA-ratio, for assessing berry maturity. The cellular metabolism enzyme activities (CMEAs) of the cell wall such as polygalacturonase (PG), cellulase (CEL), xylanase (XYL), and pectinase (PT) were minimized by applying PE + PVA-SA 2 mM coatings throughout storage duration. The accumulation of malondialdehyde (MDA) and cell wall damage, as well as the electrolyte leakage percentage (EL%), was reduced. PE + PVA-SA 2 mM maintained DPPH radical quenching activities and minimized O2− and H2O2 production rates. Collectively, these findings suggest that PE + PVA with the presence of SA as a coating treatment preserved ‘Crimson seedless’ bunches during shelf life. PE + PVA-SA 2 mM might be at least partially ascribed to the enhancement of bunches’ quality traits as well as inhibiting cell wall damage during the shelf-life period.

scholarly journals Investigation of the Physical, Chemical and Microbiological Stability of Losartan Potassium 5 mg/mL Extemporaneous Oral Liquid Suspension

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 301
Author(s):  
Lisa Foley ◽  
Jennifer Toney ◽  
James W. Barlow ◽  
Maura O’Connor ◽  
Deirdre Fitzgerald-Hughes ◽  
...  
Keyword(s):  
Shelf Life ◽  
Storage Conditions ◽  
Losartan Potassium ◽  
Microbial Count ◽  
E Coli ◽  
Physical Chemical ◽  
Liquid Suspension ◽  
Oral Liquid ◽  
Microbiological Stability ◽  
Stability Data

Extemporaneous oral liquid preparations are commonly used when there is no commercially available dosage form for adjustable dosing. In most cases, there is a lack of stability data to allow for an accurately assigned shelf life and storage conditions to give greater confidence of product safety and efficacy over its shelf life. The aim of this study was to evaluate the physical, chemical and microbiological stability of an extemporaneous oral liquid suspension of losartan potassium, 5 mg/mL, used to treat paediatric hypertension in Our Lady’s Children’s Hospital Crumlin, Ireland. The losartan content of extemporaneous oral suspensions, prepared with and without addition of water, was measured by UV and confirmed by HPLC analysis. Suspensions were stored at 4 °C and room temperature (RT) and were monitored for changes in; pH, colour, odour, re-dispersibility, Total Aerobic Microbial Count, Total Yeast and Mould Count and absence of E. coli. Results showed that suspensions prepared by both methods, stored at 4 °C and RT, were physically and microbiologically stable over 28 days. Initial losartan content of all suspensions was lower than expected at 80–81% and did not change significantly over the 28 days. HPLC and NMR did not detect degradation of losartan in the samples. Suspensions prepared in water showed 100% losartan content. The reduced initial losartan content was confirmed by HPLC and was related to the acidic pH of the suspension vehicle. Physiochemical properties of the drug are important factors for consideration in the selection of suspension vehicle for extemporaneous compounding of oral suspensions as they can influence the quality, homogeneity and efficacy of these preparations.

Salicylic acid and oxalic acid in enhancing the quality and extending the shelf life of grape cv. Thompson seedless

2021 ◽  
pp. 108201322110206
Author(s):  
TK Hazarika ◽  
Tangkasil Marak
Keyword(s):  
Salicylic Acid ◽  
Oxalic Acid ◽  
Shelf Life ◽  
Titratable Acidity ◽  
Ethylene Biosynthesis ◽  
Fungal Decay ◽  
Grape Berries ◽  
Overall Acceptability ◽  
Table Grapes ◽  
Acid Ratio

Postharvest life of table grapes is usually shortened by berry softening, berry drop, stem browning, fungal decay. Salicylic acid reduces fruit respiration and ethylene biosynthesis, during storage of fruits. Similarly, application of oxalic acid is a secure and hopeful postharvest handling technology for keeping quality and prolonging storage life of fruit. To study the effect of Salicylic acid and oxalic acid in enhancing the quality and extending the shelf life of grape, the present investigation was conducted. The grape berries were treated with Oxalic acid (OA) (1, 2, 3, 4, and 5 mM) and Salicylic acid (SA) (0.5, 1, 1.5 and 2 mM). The treatments were compared within 16th days at an interval of 4 days. Among the treatments, SA (2 mM) showed superiority in different quality attributing characters like physiological loss in weight (PLW), berry firmness, rachis browning, berry appearance, fungal decay, berry shattering, TSS, ascorbic acid, titratable acidity, total sugars, reducing sugars, TSS: acid ratio, taste, overall acceptability and shelf life. Hence, SA (2 mM) can be used as an effective strategy for maintaining quality of table grapes.

scholarly journals Improving the Biocontrol Potential of Bacterial Antagonists with Salicylic Acid against Brown Rot Disease and Impact on Nectarine Fruits Quality

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 209
Author(s):  
Nadia Lyousfi ◽  
Rachid Lahlali ◽  
Chaimaa Letrib ◽  
Zineb Belabess ◽  
Rachida Ouaabou ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Disease Severity ◽  
Mycelial Growth ◽  
Brown Rot ◽  
Antagonistic Bacteria ◽  
Biological Treatments ◽  
Rot Disease ◽  
The Impact

The main objective of this study was to evaluate the ability of both antagonistic bacteria Bacillus amyloliquefaciens (SF14) and Alcaligenes faecalis (ACBC1) used in combination with salicylic acid (SA) to effectively control brown rot disease caused by Monilinia fructigena. Four concentrations of salicylic acid (0.5%, 2%, 3.5%, and 5%) were tested under in vitro and in vivo conditions. Furthermore, the impact of biological treatments on nectarine fruit parameters’ quality, in particular, weight loss, titratable acidity, and soluble solids content, was evaluated. Regardless of the bacterium, the results indicated that all combined treatments displayed a strong inhibitory effect on the mycelial growth of M. fructigena and disease severity. Interestingly, all SA concentrations significantly improved the biocontrol activity of each antagonist. The mycelial growth inhibition rate ranged from 9.79% to 88.02% with the highest reduction rate recorded for bacterial antagonists in combination with SA at both concentrations of 0.5% and 3.5%. The in vivo results confirmed the in vitro results with a disease severity varying from 0.00% to 51.91%. A significant biocontrol improvement was obtained with both antagonistic bacteria when used in combination with SA at concentrations of 0.5% and 2%. The lowest disease severity observed with ACBC1 compared with SF14 is likely due to a rapid adaptation and increase of antagonistic bacteria population in wounded sites. The impact of all biological treatments revealed moderate significant changes in the fruit quality parameters with weight loss for several treatments. These results suggest that the improved disease control of both antagonistic bacteria was more likely directly linked to both the inhibitory effects of SA on pathogen growth and induced fruit resistance.

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