scholarly journals Analysis of Stored Wheat Grain-Associated Microbiota Reveals Biocontrol Activity among Microorganisms against Mycotoxigenic Fungi

2021 ◽  
Vol 7 (9) ◽  
pp. 781
Author(s):  
Manoj Kumar Solanki ◽  
Ahmed Abdelfattah ◽  
Sudharsan Sadhasivam ◽  
Varda Zakin ◽  
Michael Wisniewski ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Seed Quality ◽  
Bacterial Species ◽  
Rhodotorula Glutinis ◽  
Amplicon Sequencing ◽  
Wheat Grain ◽  
Wheat Grains ◽  
Stored Wheat ◽  
Antagonistic Microorganisms ◽  
Mycotoxigenic Fungi

Wheat grains are colonized by complex microbial communities that have the potential to affect seed quality and susceptibility to disease. Some of the beneficial microbes in these communities have been shown to protect plants against pathogens through antagonism. We evaluated the role of the microbiome in seed health: in particular, against mycotoxin-producing fungi. Amplicon sequencing was used to characterize the seed microbiome and determine if epiphytes and endophytes differ in their fungal and bacterial diversity and community composition. We then isolated culturable fungal and bacterial species and evaluated their antagonistic activity against mycotoxigenic fungi. The most prevalent taxa were found to be shared between the epiphytic and endophytic microbiota of stored wheat seeds. Among the isolated bacteria, Bacillus strains exhibited strong antagonistic properties against fungal pathogens with noteworthy fungal load reduction in wheat grain samples of up to a 3.59 log10 CFU/g compared to untreated controls. We also found that a strain of the yeast, Rhodotorula glutinis, isolated from wheat grains, degrades and/or metabolizes aflatoxin B1, one of the most dangerous mycotoxins that negatively affects physiological processes in animals and humans. The mycotoxin level in grain samples was significantly reduced up to 65% in the presence of the yeast strain, compared to the untreated control. Our study demonstrates that stored wheat grains are a rich source of bacterial and yeast antagonists with strong inhibitory and biodegradation potential against mycotoxigenic fungi and the mycotoxins they produce, respectively. Utilization of these antagonistic microorganisms may help reduce fungal and mycotoxin contamination, and potentially replace traditionally used synthetic chemicals.

scholarly journals EQUIPMENT AND SCIENTIFIC STUDIES OF EXPERIMENTAL DATA ON STORAGE OF WHEAT GRAIN

Food systems ◽  
2019 ◽  
Vol 2 (4) ◽  
pp. 25-30
Author(s):  
Yu. F. Markov ◽  
A. N. Buriak ◽  
L. G. Eresko
Keyword(s):  
Storage Period ◽  
Storage Conditions ◽  
Climatic Conditions ◽  
External Factors ◽  
Wheat Grain ◽  
Stored Grain ◽  
Dust Suppression ◽  
Wheat Grains ◽  
Stored Wheat ◽  
Moisture State

A complex of technical solutions is presented which makes it possible to study the influence of external factors on changes in the indicator of fat acidity value (FAV) and a range of other parameters characterizing the quality of preservation of long-term stored wheat grains in South Russia. Storage conditions (natural climatic, model) and also the effect of dust suppression treatment of grain by oil glazing that is carried out in some grain terminals in the South of Russia were taken as controlled external factors. New data has been obtained on changes in parameters of food suitability of the wheat grains during storage under model conditions with varying degrees of severity of climatic conditions in South Russia. After 6 months of storage the most considerable changes in FAV value were found for 4th class of wheat stored in natural climatic conditions with intensive insolation, the increase was 1.7 mg KOH/1 g of fat (from 7.3 mg KOH/1 g of fat up to 9.0 mg KOH/ 1g of fat). Minimum changes in the same parameter for the same storage period were observed for the wheat of the 3rd class stored in a thermostat at a stable high temperature of 35 ºС (from 11.6 mg KOH/1 g of fat to 11.5 mg KOH/1 g of fat). For wheat of the 4th class, the changes were 0.7 mg KOH/1 g of fat (from 7.8 mg KOH/1 g fat to 8.5 mg KOH/1 g of fat). Analyzes of stored wheat grains subjected to dust suppression by the oil glazing showed similar results, which allows us to state the absence of a significant effect of oil glazing on changes in wheat properties during its storage.Based on the results of the experimental analysis and a generalization of the data obtained an assumption was made on the possible reasons for the lack of pronounced trends in the data for the expected increase of FAV value in food suitability of the wheat grain when it is stored under typical model conditions of South Russia. The likely reason for this is the corresponding moisture state of grain, the water activity of grain was about 0.45. Such a low value was due, in particular, to the fact that samples of model-stored grain had a limited volume and do not reproduce the mass transfer processes that involve deep layers of the grain mass (because of the mass absence), which takes place during storage of grain in an industrial environment. The tools were proposed for the operational monitoring of the moisture state of wheat grain during storage. Moreover, a plan has been developed to expand the field of modelling storage processes in terms of varying the moisture state of the stored wheat grain samples with an assessment of the influence of moisture state on the dynamics of changes in the parameters to be controlled.

scholarly journals The Status of Insect Pests Prevailing in Stored Wheat Grain under Traditional Storages of Cheha District of Gurage Zone of South Central Ethiopia

2020 ◽  
pp. 1-7
Author(s):  
Tsehaynew Wubetu ◽  
Berhanu Hiruy
Keyword(s):  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Rhyzopertha Dominica ◽  
Southern Ethiopia ◽  
Wheat Grain ◽  
Wheat Grains ◽  
Central Ethiopia ◽  
Stored Wheat ◽  
South Central ◽  
The Status

Aim: To assess the status of the insect pests of stored wheat grains in Cheha destrict of Gurage Zone of Southern Ethiopia. Study Design: Peasant associations were selected purposefully based on intensity of wheat production, such that those peasant association growing wheat predominantly were selected for the survey, while villages, representative farmers and their storage systems were selected randomly using a nested design. Place and Duration of Study: Survey was conducted between 1, July to 30, December 2019 with the interval of one month in major wheat growing peasant associations of Cheha district of south central Ethiopia. Methodology: The assessment on abundance was made from of half kilogram of wheat grain sample taken from 135 randomly selected farmer’s storages of three peasant associations using taxonomic keys of books related to stored product insects. Results: Eight major species of insect pests consisting of four primary pests and four secondary pests belonging to five families with in two insect orders were documented. Of these pests recorded, Sitophilus oryzae, Sitophilus zeamais, Sitotroga cereallella, Tribolium castaneum and Tribolium confusum, respectively were the most prevalent and frequently occurring as they appeared between 12.74 and 33.78 individuals per 100 g of grain and as they occurred in the range between 77.78 and 92.26% per 100 g of sample wheat grain collected from the survey sites, respectively. These were followed by Cryptolestes ferrugineus, Cryptolestes pusillus and Rhyzopertha dominica which occurred between 7.26 and 10.74 individuals per 100 g of grain and in ranges between 51.85 and 66.67% per 100 g of sample wheat grain collected. Conclusion: The traditional methods and practices used by farmers were inefficient for sufficient wheat grains protection against insect pests in storages; this implies for the urgent need for designing management strategies for more effective and sustainable methods of control.

scholarly journals Rapid Detection and Identification of Mycotoxigenic Fungi and Mycotoxins in Stored Wheat Grain

Toxins ◽  
2017 ◽  
Vol 9 (10) ◽  
pp. 302 ◽  
Author(s):  
Sudharsan Sadhasivam ◽  
Malka Britzi ◽  
Varda Zakin ◽  
Moshe Kostyukovsky ◽  
Anatoly Trostanetsky ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Wheat Grain ◽  
Stored Wheat ◽  
Detection And Identification ◽  
Mycotoxigenic Fungi

Volatile organic compounds associated with postharvest fungi detected in stored wheat grain

2017 ◽  
Vol 46 (5) ◽  
pp. 483-492
Author(s):  
E. H. Barkat ◽  
B. Du ◽  
Y. Ren ◽  
G. E. St J. Hardy ◽  
Kirsty L. Bayliss
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Wheat Grain ◽  
Stored Wheat ◽  
Volatile Organic

scholarly journals Escherichia coli Clonobiome: Assessing the Strain Diversity in Feces and Urine by Deep Amplicon Sequencing

2019 ◽  
Vol 85 (23) ◽  
Author(s):  
Sofiya G. Shevchenko ◽  
Matthew Radey ◽  
Veronika Tchesnokova ◽  
Dagmara Kisiela ◽  
Evgeni V. Sokurenko
Keyword(s):  
Bacterial Species ◽  
Clonal Diversity ◽  
Amplicon Sequencing ◽  
Multidrug Resistant ◽  
Full Understanding ◽  
Highly Pathogenic ◽  
Content Type ◽  
E Coli ◽  
Strain Diversity ◽  
Healthy Carriers

ABSTRACT While microbiome studies have focused on diversity at the species level or higher, bacterial species in microbiomes are represented by different, often multiple, strains. These strains could be clonally and phenotypically very different, making assessment of strain content vital to a full understanding of microbiome function. This is especially important with respect to antibiotic-resistant strains, the clonal spread of which may be dependent on competition between them and susceptible strains from the same species. The pandemic, multidrug-resistant, and highly pathogenic Escherichia coli subclone ST131-H30 (H30) is of special interest, as it has already been found persisting in the gut and bladder in healthy people. In order to rapidly assess E. coli clonal diversity, we developed a novel method based on deep sequencing of two loci used for sequence typing, along with an algorithm for analysis of the resulting data. Using this method, we assessed fecal and urinary samples from healthy women carrying H30 and were able to uncover considerable diversity, including strains with frequencies at <1% of the E. coli population. We also found that, even in the absence of antibiotic use, H30 could completely dominate the gut and, especially, urine of healthy carriers. Our study offers a novel tool for assessing a species’ clonal diversity (clonobiome) within the microbiome, which could be useful in studying the population structure and dynamics of multidrug-resistant and/or highly pathogenic strains in their natural environments. IMPORTANCE Bacterial species in the microbiome are often represented by multiple genetically and phenotypically different strains, making insight into subspecies diversity critical to a full understanding of the microbiome, especially with respect to opportunistic pathogens. However, methods allowing efficient high-throughput clonal typing are not currently available. This study combines a conventional E. coli typing method with deep amplicon sequencing to allow analysis of many samples concurrently. While our method was developed for E. coli, it may be adapted for other species, allowing microbiome researchers to assess clonal strain diversity in natural samples. Since assessment of subspecies diversity is particularly important for understanding the spread of antibiotic resistance, we applied our method to the study of a pandemic multidrug-resistant E. coli clone. The results we present suggest that this clone could be highly competitive in healthy carriers and that the mechanisms of colonization by such clones need to be studied.

Response of Chickpea to Nitrogen, and a Comparison of the Factors Affecting Chickpea Seed Yield with those Affecting Wheat Grain Yield

1995 ◽  
Vol 31 (1) ◽  
pp. 39-47 ◽  
Author(s):  
D. J. Bonfil ◽  
M. J. Pinthus
Keyword(s):  
Seed Development ◽  
Seed Yield ◽  
Dry Matter ◽  
Growth Analysis ◽  
Field Experiments ◽  
Limiting Factors ◽  
Wheat Grain ◽  
Factors Affecting ◽  
Wheat Grains ◽  
Adjacent Field

SummaryChickpea yields in Israel are usually considerably lower than wheat yields under comparable conditions. This study aimed to examine the possible yield limiting factors in chickpeas. Increasing the availability of nitrogen during seed development by a pre-sowing application of nitrate or by nitrogen top dressing at the onset of flowering led to an increase in the percentage of nitrogen in the straw but had no significant effect on seed yield. Growth analysis of chickpeas and wheat grown in two adjacent field experiments revealed that during the fruiting period these crops accumulated similar amounts of dry matter. However, the proportion of total wheat dry matter accumulated in the wheat grains was twice the proportion of total chickpea dry matter accumulated in the chickpea seeds. It was concluded that the main intrinisic factor limiting the seed yield of chickpeas is the continuation of vegetative growth during the period of seed development, which reduces the amount of assimilate allocated to the seeds.Los factores restrictivos del rendimiento en el garbanzo

Lectins as Cytochemical Probes of the Developing Wheat Grain. IV. Demonstration of Mucilage Containing L-Fucose Associated With Roots in Ungerminated Grain

1983 ◽  
Vol 10 (5) ◽  
pp. 459 ◽  
Author(s):  
BA Baldo ◽  
AL Reid ◽  
PA Boniface
Keyword(s):  
Binding Affinity ◽  
Specific Binding ◽  
Root Cap ◽  
Cereal Grains ◽  
Wheat Grain ◽  
Physiological Maturity ◽  
Wheat Grains ◽  
Ulex Europaeus

Fluorescein-labelled lectins from Ulex europaeus and Lotus tetragonolobus, each with a specific binding affinity for L-fucose, reacted with carbohydrate material in the root cap and surrounding the roots in the embryos of developing wheat grains. The reactions were completely inhibited by preincubation of the lectins with L-fucose and were observed throughout development of the grain from 6 days post-anthesis to physiological maturity 29 days later. These findings provide the first demonstration of the location of L-fucose in the wheat grain. Although a lectin-reactive slime or mucilage containing L-fucose has been studied by others in the roots of germinated cereal grains, particularly maize, our results demonstrate that such a mucilage already occurs around the roots prior to germination.

scholarly journals Effect of black point disease on quality of wheat grain

1970 ◽  
Vol 34 (2) ◽  
pp. 181-187 ◽  
Author(s):  
PK Malaker ◽  
IH Mian ◽  
KA Bhuiyan ◽  
MMA Reza ◽  
MA Mannan
Keyword(s):  
Fungal Pathogens ◽  
Dry Matter ◽  
Bipolaris Sorokiniana ◽  
Wheat Grain ◽  
Mineral Contents ◽  
Black Point ◽  
Nutrient Contents ◽  
Calcium Magnesium ◽  
Nitrogen Phosphorus

An experiment was conducted to determine the effect of different levels of black point infection caused by Bipolaris sorokiniana and other fungal pathogens on some quality characters of wheat grain. Wheat grains were graded on a 0-5 scale based on levels of black point infection. Quality characters of grain viz., protein, fat, dry matter, ash and mineral contents under different grades were determined following standard methods. The dry matter and ash contents of grain decreased while the contents of protein and fat increased with the increasing severity of black point infection. The observations on macro and micro nutrient contents of grain indicated that the amount of nitrogen, phosphorus, calcium, magnesium, sulphur, and boron increased, whereas the contents of potassium, iron, zinc, copper and sodium decreased with the increase of black point infection. However, the reductions in copper content of black point affected grains under different grades of infection were not significant as compared to apparently healthy grains. Key Words: Black point; Bipolaris sorokiniana; grain quality; wheat. DOI: 10.3329/bjar.v34i2.5789Bangladesh J. Agril. Res. 34(2) : 181-187, June 2009

scholarly journals Comparative Analysis of Fecal Microbiota Composition Between Rheumatoid Arthritis and Osteoarthritis Patients

Genes ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 748 ◽  
Author(s):  
Jin-Young Lee ◽  
Mohamed Mannaa ◽  
Yunkyung Kim ◽  
Jehun Kim ◽  
Geun-Tae Kim ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Gut Microbiota ◽  
Gut Microbiome ◽  
Bacterial Species ◽  
Amplicon Sequencing ◽  
Fecal Microbiota ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Stool Samples ◽  
Gut Microbiota Composition

The aim of this study was to investigate differences between the gut microbiota composition in patients with rheumatoid arthritis (RA) and those with osteoarthritis (OA). Stool samples from nine RA patients and nine OA patients were collected, and DNA was extracted. The gut microbiome was assessed using 16S rRNA gene amplicon sequencing. The structures and differences in the gut microbiome between RA and OA were analyzed. The analysis of diversity revealed no differences in the complexity of samples. The RA group had a lower Bacteroidetes: Firmicutes ratio than did the OA group. Lactobacilli and Prevotella, particularly Prevotella copri, were more abundant in the RA than in the OA group, although these differences were not statistically significant. The relative abundance of Bacteroides and Bifidobacterium was lower in the RA group. At the species level, the abundance of certain bacterial species was significantly lower in the RA group, such as Fusicatenibacter saccharivorans, Dialister invisus, Clostridium leptum, Ruthenibacterium lactatiformans, Anaerotruncus colihominis, Bacteroides faecichinchillae, Harryflintia acetispora, Bacteroides acidifaciens, and Christensenella minuta. The microbial properties of the gut differed between RA and OA patients, and the RA dysbiosis revealed results similar to those of other autoimmune diseases, suggesting that a specific gut microbiota pattern is related to autoimmunity.

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