Biotransformation of Chromium (VI) via a Reductant Activity from the Fungal Strain Purpureocillium lilacinum

Industrial effluents from chromium-based products lead to chromium pollution in the environment. Several technologies have been employed for the removal of chromium (Cr) from the environment, including adsorption, ion-exchange, bioremediation, etc. In this study, we isolated a Cr (VI)-resistant fungus, Purpureocillium lilacinum, from contaminated soil, which could reduce chromium. We also characterized a reductant activity of dichromate found in the cellular fraction of the fungus: optimal pH and temperature, effect of enzymatic inhibitors and enhancers, metal ions, use of electron donors, and initial Cr (VI) and protein concentration. This study also shows possible mechanisms that could be involved in the elimination of this metal. We observed an increase in the reduction of Cr (VI) activity in the presence of NADH followed by that of formate and acetate, as electron donor. This reduction was highly inhibited by EDTA followed by NaN3 and KCN, and this activity showed the highest activity at an optimal pH of 7.0 at 37 °C with a protein concentration of 3.62 µg/mL.

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Cadmium Uptake and Growth Responses of Potted Vegetables to the Cd-Contaminated Soil Inoculated with Cd-Tolerant Purpureocillium lilacinum N1

Minerals ◽

10.3390/min11060622 ◽

2021 ◽

Vol 11 (6) ◽

pp. 622

Author(s):

Yan Deng ◽

Haonan Huang ◽

Shaodong Fu ◽

Luhua Jiang ◽

Yili Liang ◽

...

Keyword(s):

Contaminated Soil ◽

Contaminated Soils ◽

Cost Effective ◽

Fungal Strain ◽

Growth Responses ◽

Cd Uptake ◽

Purpureocillium Lilacinum ◽

Pot Experiments ◽

Cost Effective Technique ◽

Promote Plant Growth

Bioremediation of Cd- (cadmium) contaminated soil using Cd-tolerant fungus is considered an eco-friendly and cost-effective technique. In this study, we isolated one fungal strain that was hyper-tolerant to Cd from a highly polluted river and conducted pot experiments to evaluate its effects on bioremediation. We found that the fungal strain belonging to the genus, Purpureocillium lilacinum, tolerated 12,000 mg/L Cd. SEM manifested that Cd can be bioaccumulated on the crumpled mycelial surface, generating plenty of metal precipitation particles. In addition, pot experiments showed that the inoculation of P. lilacinum N1 could reduce the total Cd content in soil (2.09% in low contaminated soil and 12.56% in high contaminated soil) and greatly promote plant growth (2.16~3.13 times). Although the Cd concentration of plants was increased by 112.8% in low contaminated soil and decreased by 9.5% in highly contaminated soil with the inoculation of P. lilacinum N1, the total uptake of Cd by plants was greatly improved—1.84~3.6 times higher than that in CK groups. All our results suggest that P. lilacinum N1 is a valuable candidate for the bioremediation of Cd-contaminated soils because of its dual effects on the total Cd content in soil and Cd uptake in plants.

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Patterns of protein adsorption in ion-exchange particles and columns: evolution of protein concentration profiles during load, hold, and wash steps predicted for pore and solid diffusion mechanisms

Journal of Chromatography A ◽

10.1016/j.chroma.2021.462412 ◽

2021 ◽

pp. 462412

Author(s):

Jürgen Beck ◽

Eric van Lieres ◽

Negar Zaghi ◽

Samuel Leweke ◽

Giorgio Carta ◽

...

Keyword(s):

Ion Exchange ◽

Protein Adsorption ◽

Protein Concentration ◽

Concentration Profiles ◽

Solid Diffusion ◽

Diffusion Mechanisms

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Extruction of keratinase from the local isolate Bacillus licheniformis and partially purified and characterized

Journal of Biotechnology Research Center ◽

10.24126/jobrc.2009.3.2.68 ◽

2009 ◽

Vol 3 (2) ◽

pp. 41-52

Author(s):

Rasha T. Abdullah ◽

Abdulkareem J. Hashim ◽

JASIM M. Karhout

Keyword(s):

Enzyme Activity ◽

Bovine Serum Albumin ◽

Ion Exchange ◽

Bacillus Licheniformis ◽

Enzyme Characterization ◽

Optimal Temperature ◽

Chicken Feathers ◽

Local Isolate ◽

Cellulose Column ◽

Optimal Ph

The keratinase produced from local isolate Bacillus licheniformis was purified by two steps included precipitation by ammonium sulphate with 40% saturation; followed by ion exchange using CM-Cellulose column. The enzyme was purified to 12.6 times in the last step with an enzyme yield of 17%. Enzyme characterization results indicated that: The optimal pH for enzyme activity was 7.5 and it was stable at 7-9.5. The optimal temperature for enzyme activity was 50°C and it was stable for 30 min at 25-45 °C. Substrate specifity was tested using casein, Bovine serum albumin, gelatin, hooves, human hair, chicken feathers and wool; higher specifity was recorded using casein gave 0.6 unit /ml. The enzyme was inhibited by PMSF and metal ions like Hg+2, Fe+2, Cu+2 and Mn+2, and activated by Ca+2, Mg+2, Zn+2and Al+3.

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Kinetic and Thermodynamic Studies of Chromium (VI) Sorption by Pure and Carbonized Fluted Pumpkin waste biomass (Telfairia occidentalis Hook F.)

Journal of Nepal Chemical Society ◽

10.3126/jncs.v27i1.6436 ◽

2012 ◽

Vol 27 ◽

pp. 11-18

Author(s):

Timi Tarawou ◽

Michael Horsfall

Keyword(s):

Aqueous Solution ◽

Rate Constants ◽

Industrial Effluents ◽

Second Order ◽

Order Kinetic ◽

Waste Biomass ◽

Fluted Pumpkin ◽

Chromium Vi ◽

Telfairia Occidentalis ◽

Pseudo Second Order

The adsorption of chromium (VI) ions from aqueous solution was studied using pure and carbonized fluted pumpkin waste biomass (FPWB). The kinetic data shows a pseudo-first-order mechanism with rate constants of 1.26 × 10-2 and 1.933 × 10-2 mg g-1 min-1 for the pure and carbonized FPWB, respectively. While the pseudo-second-order mechanism has rate constants of 0.93 × 10-1 and 1.33 × 10-1 mg g-1 min-1 for the pure and carbonized waste biomass respectively. The pseudo-second order kinetic model was found to be more suitable for describing the experimental data based on the correlation coefficient values (R2) of 0.9975 and 0.9994 obtained for pure waste biomass (PWB) and carbonized waste biomass (CWB), respectively. The results obtained from this study show that PWB and CWB have very high removal capacity for chromium (VI) from aqueous solution over a range of reaction conditions. Thus, fluted pumpkin waste biomass (Telfairia occidentalis Hook F) is a potential sorbent for the treatment of industrial effluents containing chromium (VI) contaminant.DOI: http://dx.doi.org/10.3126/jncs.v27i1.6436 J. Nepal Chem. Soc., Vol. 27, 2011 11-18Uploaded date: 16 July, 2012

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Amberlite IRC-718 ion chelating resin extraction of hazardous metal Cr (VI) from aqueous solutions: equilibrium and theoretical modeling

Water Science & Technology ◽

10.2166/wst.2021.309 ◽

2021 ◽

Author(s):

Abdelhamid Addala ◽

Moussa Boudiaf ◽

Maria Elektorowicz ◽

Embarek Bentouhami ◽

Yacine Bengeurba

Keyword(s):

Ion Exchange ◽

Aqueous Solutions ◽

Exchange Process ◽

Ion Exchange Resin ◽

Exchange Capacity ◽

Chelating Resin ◽

Exchange Potential ◽

Chromium Vi ◽

Ion Exchange Process ◽

Hazardous Metal

Abstract Under varied conditions, the IRC 718 ion-exchange resin is used to extract chromium (VI) ions from aqueous solutions. On chromium (VI) removal effectiveness, the effects of adsorption dosage, contact time, beginning metal concentration, and pH were examined. The batch ion exchange process reached equilibrium after around 90 minutes of interaction. With an initial chromium (VI) concentration of 0.5 mg/dm3, the pH-dependent ion-exchange mechanism revealed maximal removal in the pH 2.0–10 range . The adsorption mechanism occurs between Cr(VI) determined as the electron acceptor, and IRC 718 determined as the electron donor. The equilibrium ion-exchange potential and ion transfer quantities for Amberlite IRC 718 were calculated using the Langmuir adsorption isotherm model. The overall ion exchange capacity of the resin was determined to be 187.72 mg of chromium (VI)/g of resin at an ideal pH of 6.0.

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Synthesis of ion-exchange membranes for electrodialytic treatment of industrial effluents

Desalination ◽

10.1016/0011-9164(83)87177-x ◽

1983 ◽

Vol 46 (1-3) ◽

pp. 353-360 ◽

Cited By ~ 5

Author(s):

B. Chakravorty ◽

R.N. Mukherjee ◽

S. Basu

Keyword(s):

Ion Exchange ◽

Industrial Effluents ◽

Ion Exchange Membranes

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Synthesis, Characterization and Ion Exchange Behavior of Polyaniline Stannic Silicomolybdate, an Organic–Inorganic Composite Material: Quantitative Separation of Pb2+ Ions from Industrial Effluents

Journal of Inorganic and Organometallic Polymers and Materials ◽

10.1007/s10904-010-9423-0 ◽

2010 ◽

Vol 21 (1) ◽

pp. 25-35 ◽

Cited By ~ 8

Author(s):

Syed Ashfaq Nabi ◽

Sajad Ahmad Ganai ◽

Amjad Mumtaz Khan

Keyword(s):

Composite Material ◽

Ion Exchange ◽

Industrial Effluents ◽

Quantitative Separation ◽

Inorganic Composite

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Adsorption and ion exchange of some groundwater anion contaminants in an amine modified coconut coir

Water Science & Technology ◽

10.2166/wst.1997.0264 ◽

1997 ◽

Vol 35 (7) ◽

pp. 89-95 ◽

Cited By ~ 21

Author(s):

Aloysius U. Baes ◽

Tetsuji Okuda ◽

Wataru Nishijima ◽

Eiji Shoto ◽

Mitsumasa Okada

Keyword(s):

Ion Exchange ◽

Low Cost ◽

Exchange Capacity ◽

Batch Adsorption ◽

Ion Exchange Capacity ◽

Strong Base ◽

Chromium Vi ◽

Freundlich Equation ◽

Adsorption Data ◽

Coconut Coir

The adsorption of nitrate, chromium (VI), arsenic (V) and selenium (VI) anions in an amine modified coconut coir (MCC-AE : with secondary and tertiary amine functionality) were studied to determine the capability of this easily prepared and low-cost material in removing typical groundwater anion contaminants. Batch adsorption-ion exchange experiments were conducted using 200 mg MCC-AE, initially containing chloride as the resident anion, and 50 ml of different anion-containing water of varying concentrations. It is presumed, at this low pH, that only SeO42− remained as a divalent anion, while monovalent species H2AsO4− and HCrO4− predominated in their respective exchanging ion solutions. The adsorption data were fitted using the Freundlich equation and maximum adsorption for each anion was estimated using their respective Freundlich equation constants. MCC-AE exhibited preference for divalent Cr (VI) and Se (VI) anions compared with the Cl− resident ion. Maximum As (V) adsorption was 0.086 mmol/g, while maximum adsorption of Cr (VI), NO3− and Se (VI) anions was 0.327 mmol/g, 0.459 mmol/g, and 0.222 mmol/g, respectively. The ion exchange capacity of MCC-AE is estimated, based on its exchange capacity for nitrate, to be within 0.46 mmol of positive charges per gram. Similar adsorption experiments were conducted for comparison using commercial chloride-form Amberlite IRA-900 strong base (quaternary amine functionality) anion exchanger, with an exchange capacity of 4.2 meq/g. Maximum adsorption of the different ions in IRA-900 was about 3 times higher for NO3−, 9 times higher for Se (VI), 10 times higher for As (V) and 9 times higher for Cr (VI), than that in MCC-AE. Differences in the ion exchange behavior of MCC-AE and IRA-900 were probably due to the different amine functionalities in the two exchangers. The results suggest that MCC-AE may be used as a low-cost alternative adsorbent/ion exchanger for treatment of anion contaminants in groundwater.

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