scholarly journals High Efficiency In Vitro Wound Healing of Dictyophora indusiata Extracts via Anti-Inflammatory and Collagen Stimulating (MMP-2 Inhibition) Mechanisms

2021 ◽  
Vol 7 (12) ◽  
pp. 1100
Author(s):  
Yasir Nazir ◽  
Pichchapa Linsaenkart ◽  
Chiranan Khantham ◽  
Tanakarn Chaitep ◽  
Pensak Jantrawut ◽  
...  
Keyword(s):  
Wound Healing ◽  
Interleukin 1 ◽  
Healing Process ◽  
Matrix Metalloproteinase 2 ◽  
Nucleotide Sequencing ◽  
Wound Healing Process ◽  
Healing Effect ◽  
Wound Healing Effect ◽  
Anti Inflammatory ◽  
Collagen Stimulation

Dictyophora indusiata or Phallus indusiatus is widely used as not only traditional medicine, functional foods, but also, skin care agents. Biological activities of the fruiting body from D. indusiata were widely reported, while the studies on the application of immature bamboo mushroom extracts were limited especially in the wound healing effect. Wound healing process composed of 4 stages including hemostasis, inflammation, proliferation, and remodelling. This study divided the egg stage of bamboo mushroom into 3 parts: peel and green mixture (PGW), core (CW), and whole mushroom (WW). Then, aqueous extracts were investigated for their nucleotide sequencing, biological compound contents, and wound healing effect. The anti-inflammatory determination via the levels of cytokine releasing from macrophages, and the collagen stimulation activity on fibroblasts by matrix metalloproteinase-2 (MMP-2) inhibitory activity were determined to serve for the wound healing process promotion in the stage 2–4 (wound inflammation, proliferation, and remodelling of the skin). All D. indusiata extracts showed good antioxidant potential, significantly anti-inflammatory activity in the decreasing of the nitric oxide (NO), interleukin-1 (IL-1), interleukin-1 (IL-6), and tumour necrosis factor-α (TNF-α) secretion from macrophage cells (p < 0.05), and the effective collagen stimulation via MMP-2 inhibition. In particular, CW extract containing high content of catechin (68.761 ± 0.010 mg/g extract) which could significantly suppress NO secretion (0.06 ± 0.02 µmol/L) better than the standard anti-inflammatory drug diclofenac (0.12 ± 0.02 µmol/L) and their MMP-2 inhibition (41.33 ± 9.44%) was comparable to L-ascorbic acid (50.65 ± 2.53%). These findings support that CW of D. indusiata could be an essential natural active ingredient for skin wound healing pharmaceutical products.

scholarly journals Knockdown of Tcf3 enhances the wound healing effect of bone marrow mesenchymal stem cells in rats

2019 ◽  
Vol 39 (8) ◽  
Author(s):  
Bin He ◽  
Jia Chen ◽  
Liang Liu ◽  
Hao Wang ◽  
Shaohua Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Healing Process ◽  
Wound Healing Process ◽  
Down Regulation ◽  
Healing Effect ◽  
Marrow Mesenchymal Stem Cells ◽  
Wound Healing Effect

AbstractThe aim of the present study was to explore the wound healing effect of Tcf3 in rat bone marrow mesenchymal stem cells (BMSCs) and their effects on wound healing. Epidermal growth factor (EGF) and fibroblast growth factors (FGFs) were used to induce BMSCs differentiation into epithelial-like cells. Western Blotting analysis and RT-qPCR were performed to assess the expression levels of Tcf3 and the markers of epithelial-like cells, such as Cytokeratin-18 (CK-18), CK-19 and P63. Cell counting kit-8 (CCK-8) and clone formation assay were carried out to detect cell viability. Immunohistochemistry and HE staining were used to assess the level of Tcf3 protein and skin repair degree, respectively. Rat wound healing model was built to evaluate the effects of BMSCs with altered expression of Tcf3 on wound healing. Results showed that EGF and FGFs stimulation increased the expression of CK-18, CK-19 and P63, improved BMSCs viability, but decreased the expression of Tcf3. Knockdown of Tcf3 in BMSCs increased CK-18, CK-19 and P63 expression and improved cell proliferation, as well as accelerated wound healing process. Moreover, inhibition of Wnt/β-catenin signaling weakened the effect of Tcf3 down-regulation on BMSCs proliferation enhancement. And inhibition of Notch1 signaling impeded the epithelial-like cell differentiation of BMSCs induced by Tcf3 down-regulation. Our study reveals that knockdown of Tcf3 enhances the wound healing process of BMSCs in rat, which provides new approach for accelerating skin regeneration.

scholarly journals Investigation of Wound Healing Effect of Acalypha Indica Extract in Sprague Dawley Rats

2019 ◽  
Vol 12 (04) ◽  
pp. 1857-1865 ◽  
Author(s):  
Ng Kit Yeng ◽  
Rumaizi Shaari ◽  
Muhammad Luqman Nordin ◽  
Jasni Sabri
Keyword(s):  
Wound Healing ◽  
Body Weight ◽  
Healing Process ◽  
The Body ◽  
Wound Healing Process ◽  
Collagen Deposition ◽  
Sprague Dawley ◽  
Healing Effect ◽  
Wound Healing Effect ◽  
Acalypha Indica

This study was conducted to investigate the wound healing effect of the ethanol extract of Acalypha indica in different parts of the body. Two set of cutaneous wounds were created on an individual rat. A set of wound consisted of a 5mm wound on dorsum and a 5mm wound on hind limb. The wound on left side was treated while the right side acted as control. Three rats were euthanized every 3 days, which was on day 3, 6, 9, 12 and 15 after wounding. The body weight of the rats were recorded and skin samples were obtained for histopathology. Parameters such as epithelialization, angiogenesis, number of PMNLs and macrophages, fibroblast and collagen deposition were used in evaluating the wound healing effect. A semi quantitative scoring system was used to grade the parameters. Results showed increasing of the body weight of the rats indicated that rats were not affected by the wound induction. Gross findings revealed that the percentage of wound contraction was higher in treated wound, indicating that the wound healing process was improved by the application of the extract. Histological findings showed that the period of epithelialization was shorter in the treated wound. The fibroblastic activity, collagen deposition and angiogenic activity were also much higher in the treated wound. In conclusion, A. indica extract has good wound healing effect and plausibly can be further commercialised for wound healing treatment.

scholarly journals Jatropha Neopauciflora Pax Latex Exhibits Wound-Healing Effect in Normal and Diabetic Mice

2021 ◽  
Vol 26 ◽  
pp. 2515690X2098676
Author(s):  
Ana Bertha Hernandez-Hernandez ◽  
Francisco Javier Alarcon-Aguilar ◽  
Mario Garcia-Lorenzana ◽  
Marco Aurelio Rodriguez-Monroy ◽  
Maria Margarita Canales-Martinez
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Wound Healing Process ◽  
Diabetic Mice ◽  
Wound Area ◽  
Oral Infections ◽  
Patients With Diabetes ◽  
Exclusion Chromatography ◽  
Wound Healing Effect ◽  
Molecular Exclusion Chromatography

Jatropha neopauciflora is an endemic species of Mexico. Its latex is used to treat wounds, scarring, oral infections, and loose teeth. To date, there are no studies that validate at a morphological level a wound-healing use in diabetes. The present research aimed to evaluate the wound-healing capacity of the latex of J. neopauciflora in the skin of healthy and streptozotocin-induced diabetic mice. Also, a chemical analysis of the latex through molecular exclusion chromatography and HPLC were performed. Male mice ( Mus musculus) of 7-week-old CD1 strain were used. Groups of healthy and diabetic mice were formed. A longitudinal cut of 1 cm was performed on the depilated skin. All treatments were topically applied to the wound area twice a day for ten days. At the end of the experiments, the skin sections were obtained from the wound area and stained with Hematoxylin-Eosin. Then we counted the number of active fibroblasts in all the experimental groups. In normal mice, the latex accelerated the wound-healing process and decreased the number of active fibroblasts, similarly to Recoveron. In diabetic mice, the latex and Recoveron increased the number of active fibroblasts. In normal and diabetic mice, a thin and orderly epidermis was observed. Molecular exclusion chromatography exhibited 58 fractions, 14 of which were subjected to HPLC, to detect catechin, a flavonoid with antioxidant, antimicrobial, and anti-inflammatory properties. J. neopauciflora latex can be useful for wound treatment in patients with diabetes mellitus because it accelerates and promotes the wound-healing process.

scholarly journals Anti-inflammatory activity of Schrebera Swietenioides Roxb. in cream formulation on CD68 expression in mice skin wound

2019 ◽  
Vol 9 (5) ◽  
pp. 39-42
Author(s):  
Piush Sharma ◽  
Charanjeet Singh
Keyword(s):  
Wound Healing ◽  
Surgical Procedures ◽  
Healing Process ◽  
Skin Incision ◽  
Skin Wound ◽  
Inflammatory Activity ◽  
Wound Healing Process ◽  
Male Mice ◽  
Entire Surface ◽  
Anti Inflammatory

Aim: The purpose of this study was to determine the potential of Schrebera Swietenioides alcohol leaf extract in cream composition in the expression of immunoglobulin CD68 (macrophage) during the wound healing process in the inflammatory stage of mice skin. Materials and Methods: Amount of 12 two-months-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. The mice were allotted into four groups of each, entire surface of each group wound covered by base cream control, sulfadiazine 0.1% cream, alcoholic leaves extract of Schrebera Swietenioides cream 10% and, 15%, respectively. All experiments were performed twice a day for 3 days. The wound healing was assayed in stained histological sections in immunohistochemical of the wounds. CD68 expression was investigated under a microscope. Amount of 12-month-old male mice between 30 and 40 g was used. For surgical procedures, a 2.0 cm skin lesion was also performed under the skin on the vertebrae surrounding each animal. Treatment was performed under local anesthetic with procaine cream. Rats were assigned to four groups each, the entire surface of each group of lesions covered by control cream, 0.1% sulfadiazine cream, alcohol leaf from Schrebera Swietenioides cream respectively at 10% and at 15%. All tests were performed twice a day for 3 days. The lesions were analyzed in sections of tissue with staining of wound immunochemistry. The expression of CD68 was studied under a microscope. Results: The results showed that the cream from the 10% and 15% alcoholic leaves extract of Schrebera Swietenioides revealed moderate immune reaction to CD68 on wound healing. Conclusion: We concluded that the alcoholic leaves extract cream of Schrebera Swietenioides possesses anti-inflammatory activity in wound healing process of mice skin.

scholarly journals Kinetics of LYVE-1-Positive M2-Like Macrophages in Developing and Repairing Dental Pulp in Vivo and Their Pro-Angiogenic Activity in Vitro

2022 ◽  
Author(s):  
Kieu Quoc Thoai ◽  
Kento Tazawa ◽  
Nobuyuki Kawashima ◽  
Sonoko Noda ◽  
Mayuko Fujii ◽  
...  
Keyword(s):  
Wound Healing ◽  
Dental Pulp ◽  
Umbilical Vein ◽  
Healing Process ◽  
Tissue Remodeling ◽  
Interleukin 4 ◽  
Matrix Metalloproteinase 2 ◽  
Wound Healing Process ◽  
Post Injury

Abstract Tissue-resident macrophages expressing lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) are found in multiple tissues and organs. We aimed to evaluate the dynamics and biological function of LYVE-1+ macrophages in dental pulp during post-injury tissue remodeling. Immunofluorescence staining of mouse embryos revealed that LYVE-1+ macrophages colonized dental pulp before birth. In mature rat molar dental pulp, LYVE-1+ macrophages were the main subset of macrophages expressing CD163, an M2 marker, and were distributed throughout the tissue. In response to dental pulp injury induced by cavity preparation, LYVE-1+ macrophages quickly disappeared from the affected area of the pulp and gradually repopulated during the wound healing process. RAW264.7 mouse macrophages cultured with a mixture of macrophage colony-stimulating factor, interleukin-4, and dexamethasone increased LYVE-1 expression, whereas lipopolysaccharide-stimulation decreased LYVE-1 expression. Enforced expression of Lyve1 in RAW264.7 cells resulted in increased mRNA expression of matrix metalloproteinase 2 (Mmp2), Mmp9, and vascular endothelial growth factor A (Vegfa). Lyve1-expressing macrophages promoted the migration and tube formation of human umbilical vein endothelial cells. In conclusion, LYVE-1+ tissue-resident M2-like macrophages in dental pulp showed dynamism in response to pulp injury, and possibly play an important role in angiogenesis during wound healing and tissue remodeling.

Composite Nano-Titanium Oxide-Chitosan Artificial Skin Exhibits Strong Wound-Healing Effect-An Approach with Anti-Inflammatory and Bactericidal Kinetics

2007 ◽  
Vol 8 (4) ◽  
pp. 316-327 ◽  
Author(s):  
Chiung-Chi Peng ◽  
Ming-Hua Yang ◽  
Wen-Ta Chiu ◽  
Chun-Hung Chiu ◽  
Chi-Shen Yang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Titanium Oxide ◽  
Artificial Skin ◽  
Healing Effect ◽  
Wound Healing Effect ◽  
Anti Inflammatory

Wound healing effect benzosulfonate 1-ethyl-3-methyl-4,5-bis(n-methylcarbamoyl) imidazolium

2020 ◽  
Vol 18 (3) ◽  
pp. 229-235
Author(s):  
Lydmila K. Khnychenko ◽  
Elena N. Selina ◽  
Olga M. Rodionova ◽  
Levon B. Piotrovskiy ◽  
Petr D. Shabanov
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Experimental Models ◽  
Skin Wound ◽  
Complete Healing ◽  
Male Rats ◽  
Skin Wounds ◽  
Skin Wound Healing ◽  
Healing Effect ◽  
Wound Healing Effect

Materials and methods. In experiments on 180 male rats weighing 180200 g, the wound-healing effect of derivatives of imidazole (IEM-1181) was evaluated as a 10% ointment on models of aseptic full-layer linear and planar skin wounds. Results. It was found that the compound IEM-1181 in the form of 10% ointment has a pronounced on skin wound healing effect, manifested in the qualitative features of the regenerative healing process. With local application of the ointment containing the tested compound, the strength of the tissue formed on the site of a full-layer linear skin wound was increased when its edges were stretched, and the healing time of full-layer planar wounds was reduced. The morphological picture of the tissue formed at the site of the wound defect corresponded to complete healing by primary tension with complete epithelization of the surface and squamous cell differentiation of the epithelial regenerate without signs of inflammation and scar formation. Conclusion. The results of the study on experimental models of full-layer linear and planar skin wounds indicate that the wound healing effect of the tested ointment is due to the anti-inflammatory activity of the IEM-1181 compound

scholarly journals The Wound Healing Property of N-Methyl-(2S,4R)-trans-4-Hydroxy-L-Proline from Sideroxylon obtusifolium is Related to its Anti-Inflammatory and Antioxidant Actions

2019 ◽  
Vol 24 ◽  
pp. 2515690X1986516 ◽  
Author(s):  
Pedro Everson Alexandre de Aquino ◽  
Tamiris de Fátima Goebel de Souza ◽  
Flávia Almeida Santos ◽  
Ana Flávia Seraine Custódio Viana ◽  
Bianca Oliveira Louchard ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Wound Healing Process ◽  
Collagen Deposition ◽  
Control Groups ◽  
Wound Induction ◽  
Extracellular Matrix Components ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
And Control

Wound healing involves the interaction of blood cells, proteins, proteases, growth factors, and extracellular matrix components. Inflammation is one of the first events occurring during this process. Previously, we showed that the N-Methyl-(2S,4R)-trans-4-Hydroxy-L-Proline (NMP) from Sideroxylon obtusifolium leaves (a Brazilian medicinal species) presents an anti-inflammatory action. Considering inflammation as an important event in the wound healing process, the objectives were to investigate the topical effects of the NMP gel on a mice wound-induced model. Male Swiss mice were divided into 4 groups: Sham (surgical procedure only), Control (gel-base treated), and 3% or 10% NMP gel-treated groups. Measurements of wound areas and microscopic analyses (HE [hematoxylin-eosin] and PSR [picrosirius red] stainings) were carried out, at the 7th and 12th, days after the wound induction. Furthermore, immunohistochemical assays for iNOS (inducible nitric oxide synthase) and COX-2 (cyclooxygenase-2) and biochemical measurements for TBARS (thiobarbituric acid reactive substances), GSH (glutathione), and myeloperoxidase (MPO) were also performed, at the second day after the wound induction. The work showed that NMP decreases the wound areas, after topical application, relatively to the Sham and Control groups. In addition, microscopic alterations were reduced and collagen deposition was increased, at the 7th and 12th days, in the 10% NMP group. While iNOS and COX-2 immunostainings and GSH contents increased, in relation to the Sham and Control groups, TBARS and MPO decreased. Altogether, the results showed NMP to improve the wound healing process, by upregulating iNOS and COX-2 activities, reducing lipid peroxidation and MPO activity, and increasing GSH contents. In addition, NMP certainly contributes to the increased collagen deposition. These data may stimulate translational studies dealing with the possible use of NMP from Sideroxylon obtusifolium or from other sources for the management of wound healing.

scholarly journals Antibacterial and anti-inflammatory activity of extracts and major constituents derived from Stachytarpheta indica Linn. leaves and their potential implications for wound healing

2021 ◽  
Author(s):  
Vajira Asela Agampodi ◽  
Peter Katavic ◽  
Christopher Collet ◽  
Trudi Collet
Keyword(s):  
Wound Healing ◽  
Plant Extracts ◽  
Colorimetric Assay ◽  
Morphological Changes ◽  
Healing Process ◽  
Human Keratinocytes ◽  
Wound Healing Process ◽  
Keratinocyte Proliferation ◽  
Anti Inflammatory ◽  
Treatment Of Wounds

Abstract Wounds of various types continue to have a severe socioeconomic impact on the cost of health care. Globally, there has been increased interest surrounding the identification of bioactive compounds that promote or modulate the wound healing process. Stachytarpheta indica Linn. is traditionally used to heal wounds and relieve inflammation, however, the theorised pharmacological properties have not yet been scientifically validated. In this study, dried and ground plant leaves were extracted with water and methanol, which were then subjected to various analyses. Antimicrobial activity of the plant extracts and isolated compounds was determined using well diffusion assays, while the minimum inhibitory concentrations were determined with a colorimetric assay. Morphological changes of human keratinocytes in response to plant extracts were observed with differential interference contrast microscope imaging. Cell viability, proliferation and migratory effects post-treatment with the plant extracts were also evaluated via colorimetric cytotoxicity assays and a real-time cell analyser protocol. Anti-inflammatory effects of plant extracts and isolated compounds were evaluated by flow cytometry and cyclooxygenase and lipoxygenase enzyme inhibition assays. Three active compounds i.e. ipolamiide, verbascoside and iso-verbascoside, were isolated from S. indica leaves. Verbascoside demonstrated broad-range antibacterial activity and imposed strong inhibition at 9.77 μg/mL against Staphylococci spp. S. indica extracts (0.1-0.2 mg/mL) were shown to improve human keratinocyte proliferation up to 60 % and induce morphological changes by producing cytoplasmic projections at concentrations higher than 0.4 mg/mL. Plant extracts (6.25-100 μg/mL) and individual compounds (3.125-50 μg/mL) elicited strong anti-inflammatory effects by suppressing the expression of interleukin-8 and inhibiting cyclooxygenase-1 and 5-lipoxygenase enzymes. Collectively, these results indicate that plant extracts and isolated compounds derived from S. indica have the potential to inhibit bacterial growth, promote tissue regeneration and reduce inflammation, hence, potentially providing the basis for a novel therapeutic for the treatment of wounds.

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