scholarly journals Thiolation of Myco-Synthesized Fe3O4-NPs: A Novel Promising Tool for Penicillium expansium Laccase Immobilization to Decolorize Textile Dyes and as an Application for Anticancer Agent

2022 ◽  
Vol 8 (1) ◽  
pp. 71
Author(s):  
Hamed M. El-Shora ◽  
Aiah M. Khateb ◽  
Doaa B. Darwish ◽  
Reyad M. El-Sharkawy
Keyword(s):  
Textile Wastewater ◽  
Chemotherapeutic Agents ◽  
Promising Tool ◽  
Fe3o4 Nps ◽  
Immobilized Biocatalyst ◽  
Improved Stability ◽  
Laccase Immobilization ◽  
And Storage ◽  
Magnetic Nanoparticles Fe3o4 ◽  
Insight Into

Environmental pollution due to the continuous uncontrolled discharge of toxic dyes into the water bodies provides insight into the need to eliminate pollutants prior to discharge is significantly needed. Recently, the combination of conventional chemotherapeutic agents and nanoparticles has attracted considerable attention. Herein, the magnetic nanoparticles (Fe3O4-NPs) were synthesized using metabolites of Aspergillus niger. Further, the surfaces of Fe3O4-NPs were functionalized using 3-mercaptoproionic acid as confirmed by XRD, TEM, and SEM analyses. A purified P. expansum laccase was immobilized onto Fe3O4/3-MPA-SH and then the developed immobilized laccase (Fe3O4/3-MPA-S-S-laccase) was applied to achieve redox-mediated degradation of different dyes. The Fe3O4/3-MPA-S-S-laccase exhibited notably improved stability toward pH, temperature, organic solvents, and storage periods. The Fe3O4/3-MPA-S-S-laccase exhibited appropriate operational stability while retaining 84.34% of its initial activity after 10 cycles. The catalytic affinity (Kcat/Km) of the immobilized biocatalyst was increased above 10-fold. The experimental data showed remarkable improvement in the dyes’ decolorization using the immobilized biocatalyst in the presence of a redox mediator in seven successive cycles. Thus, the prepared novel nanocomposite-laccase can be applied as an alternative promising strategy for bioremediation of textile wastewater. The cytotoxic level of carboplatin and Fe3O4-NPs singly or in combination on various cell lines was concentration-dependent.

Quantitative Analysis Of X-Ray Images From Geological Materials

1990 ◽  
Vol 48 (2) ◽  
pp. 244-245
Author(s):  
J. M. Paque ◽  
R. Browning ◽  
P. L. King ◽  
P. Pianetta
Keyword(s):  
Quantitative Analysis ◽  
Bulk Composition ◽  
Principal Component ◽  
Analytical Description ◽  
Bulk Sample ◽  
Geological Samples ◽  
X Ray ◽  
Software And Hardware ◽  
And Storage ◽  
Insight Into

Geological samples typically contain many minerals (phases) with multiple element compositions. A complete analytical description should give the number of phases present, the volume occupied by each phase in the bulk sample, the average and range of composition of each phase, and the bulk composition of the sample. A practical approach to providing such a complete description is from quantitative analysis of multi-elemental x-ray images.With the advances in recent years in the speed and storage capabilities of laboratory computers, large quantities of data can be efficiently manipulated. Commercial software and hardware presently available allow simultaneous collection of multiple x-ray images from a sample (up to 16 for the Kevex Delta system). Thus, high resolution x-ray images of the majority of the detectable elements in a sample can be collected. The use of statistical techniques, including principal component analysis (PCA), can provide insight into mineral phase composition and the distribution of minerals within a sample.

A Review of Electroporation-based Antitumor Skin Therapies and Investigation of Betulinic Acid-loaded Ointment

2018 ◽  
Vol 18 (5) ◽  
pp. 693-701
Author(s):  
Monika Bakonyi ◽  
Szilvia Berko ◽  
Gabor Eros ◽  
Gabor Varju ◽  
Cristina A. Dehelean ◽  
...  
Keyword(s):  
Betulinic Acid ◽  
High Efficiency ◽  
Antitumor Agents ◽  
Ex Vivo ◽  
Mouse Skin ◽  
Chemotherapeutic Agents ◽  
Hairless Mouse ◽  
Promising Tool ◽  
Acid Formulation ◽  
Invasive Method

Background: Electrochemotherapy is a novel treatment for cutaneous and subcutaneous tumors utilizing the combination of electroporation and chemotherapeutic agents. Since tumors have an increasing incidence nowadays as a result of environmental and genetic factors, electrochemotherapy could be a promising treatment for cancer patients. Objective: The aim of this article is to summarize the novel knowledge about the use of electroporation for antitumor treatments and to present a new application of electrochemotherapy with a well-known plant derived antitumor drug betulinic acid. For the review we have searched the databases of scientific and medical research to collect the available publications about the use of electrochemotherapy in the treatment of various types of cancer. Method: By the utilization of the available knowledge, we investigated the effect of electroporation on the penetration of a topically applied betulinic acid formulation into the skin by ex vivo Raman spectroscopy on hairless mouse skin. Results: Raman measurements have demonstrated that the penetration depth of betulinic acid can be remarkably ameliorated by the use of electroporation, so this protocol can be a possibility for the treatment of deeper localized cancer nodules. Furthermore, it proved the influence of various treatment times, since they caused different spatial distributions of the drug in the skin. Conclusion: The review demonstrates that electrochemotherapy is a promising tool to treat different kinds of tumors with high efficiency and with only a few moderate adverse effects. Moreover, it presents a non-invasive method to enhance the penetration of antitumor agents, which can offer novel prospects for antitumor therapies.

A NEW INSIGHT INTO THE TRANSPORT AND STORAGE OF MAGMA IN SUBVOLCANIC SYSTEMS: A CASE STUDY FROM THE REYDARA LACCOLITH, SE ICELAND

2019 ◽  
Author(s):  
Michael Petronis ◽  
◽  
Vincent Twomey ◽  
William McCarthy ◽  
Craig MaGee
Keyword(s):  
And Storage ◽  
Insight Into

scholarly journals Focused Ultrasound Strategies for Brain Tumor Therapy

2019 ◽  
Vol 19 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Adomas Bunevicius ◽  
Nathan Judson McDannold ◽  
Alexandra J Golby
Keyword(s):  
Brain Tumors ◽  
Viral Vectors ◽  
Focused Ultrasound ◽  
Tumor Therapy ◽  
Drug Distribution ◽  
Low Frequency ◽  
Tissue Level ◽  
Chemotherapeutic Agents ◽  
Promising Tool ◽  
Investigational Agents

Abstract BACKGROUND A key challenge in the medical treatment of brain tumors is the limited penetration of most chemotherapeutic agents across the blood–brain barrier (BBB) into the tumor and the infiltrative margin around the tumor. Magnetic resonance-guided focused ultrasound (MRgFUS) is a promising tool to enhance the delivery of chemotherapeutic agents into brain tumors. OBJECTIVE To review the mechanism of FUS, preclinical evidence, and clinical studies that used low-frequency FUS for a BBB opening in gliomas. METHODS Literature review. RESULTS The potential of externally delivered low-intensity ultrasound for a temporally and spatially precise and predictable disruption of the BBB has been investigated for over a decade, yielding extensive preclinical literature demonstrating that FUS can disrupt the BBB in a spatially targeted and temporally reversible manner. Studies in animal models documented that FUS enhanced the delivery of numerous chemotherapeutic and investigational agents across the BBB and into brain tumors, including temozolomide, bevacizumab, 1,3-bis (2-chloroethyl)-1-nitrosourea, doxorubicin, viral vectors, and cells. Chemotherapeutic interventions combined with FUS slowed tumor progression and improved animal survival. Recent advances of MRgFUS systems allow precise, temporally and spatially controllable, and safe transcranial delivery of ultrasound energy. Initial clinical evidence in glioma patients has shown the efficacy of MRgFUS in disrupting the BBB, as demonstrated by an enhanced gadolinium penetration. CONCLUSION Thus far, a temporary disruption of the BBB followed by the administration of chemotherapy has been both feasible and safe. Further studies are needed to determine the actual drug delivery, including the drug distribution at a tissue-level scale, as well as effects on tumor growth and patient prognosis.

Qualität durch robuste Prozessketten steigern – Potentiale für die Umformtechnik mit Industrie 4.0/Quality improvement through robust process chains – Potentials for metal forming by Industrie 4.0 approaches

2015 ◽  
Vol 105 (10) ◽  
pp. 674-679
Author(s):  
P. Groche ◽  
J. Schreiner ◽  
J. Hohmann ◽  
S. Höhr ◽  
A. Lechler
Keyword(s):  
Value Chain ◽  
Metal Forming ◽  
Deep Understanding ◽  
Industrie 4.0 ◽  
Process Data ◽  
Process Chains ◽  
Recording Analysis ◽  
Robust Process ◽  
And Storage ◽  
Insight Into

Industrie 4.0 gestattet transparente sowie sachgerecht angepasste Wertschöpfungsketten. Dazu ist es nötig, ein tiefgreifendes Prozessverständnis zu besitzen sowie die Aufnahme, Auswertung und Speicherung der relevanten Daten zu bewerkstelligen. Der Beitrag gibt einen Einblick in Industrie 4.0-Ansätze in der Umformtechnik und zeigt ausgewählte Ergebnisse aus dem Verbundprojekt „RobIN 4.0“.   Industrie 4.0 opens the possibility to realize a monitoring and qualified adaption along the entire value chain. Prerequisites for this include a deep understanding of the process as well as achieving the recording, analysis and storage of relevant process data. This paper gives an insight into Industrie 4.0 approaches for the forming industry and presents selected results of the RobIN 4.0-project.

Metal ion – biomolecule interactions. X. Synthesis, spectroscopic and magnetic resonance investigations of methylmercury(II) complexes of the sulfur modified nucleosides 6- mercaptopurine riboside and 2-amino-6-mercaptopurine riboside

1986 ◽  
Vol 64 (3) ◽  
pp. 442-448 ◽  
Author(s):  
E. Buncel ◽  
R. Kumar ◽  
A. R. Norris
Keyword(s):  
Aqueous Solution ◽  
Metal Ion ◽  
Chemotherapeutic Agents ◽  
Modified Nucleosides ◽  
Coupling Constants ◽  
Heterocyclic Ligands ◽  
Methylmercury Poisoning ◽  
Insight Into ◽  
C Nmr ◽  
Ligand Bond

A number of methylmercurated complexes of 6-mercaptopurine riboside and 2-amino-6-mercaptopurine riboside (6-MNucH2) containing S-bound CH3Hg(II) in neutral and cationic complexes (as in [CH3Hg(6-MNucH)] and [CH3Hg(6-MNucH2)]NO3), S- and N-bound CH3Hg(II) (as in [(CH3Hg)2(6-MNucH)]NO3), and S-, N-, C-bound CH3Hg(II) (as in [(CH3Hg)3(6-MNuc)]NO3) have been prepared in aqueous solution at appropriate pH and mole ratios of the constituents. The complexes were characterized by means of 1H and 13C nmr and ir spectroscopy and elemental analysis. Formation of C-bound methylmercurated species extends our previous results obtained with xanthosine, inosine, and imidazole derivatives, and substantiates our proposal that activation through electrophilic coordination at N(7) is a requirement for C(8)—H abstraction. 2J(1H–199Hg) coupling constants, measured in (CD3)2SO for a number of CH3Hg(II) complexes of N-, S-, and C-donor heterocyclic ligands, including the 6-mercaptopurine riboside of the present study, correlate well with the 1J(13C–199Hg) coupling constants, according to 1J = 8.4602J − 155.6. The significance of this correlation in terms of the strength of the Hg–ligand bond is considered. The results could provide insight into the apparent selectivity of binding of CH3Hg(II) by bio-ligands, as well as in the design of chemotherapeutic agents for the treatment of methylmercury poisoning.

scholarly journals Mechanism-Based Model of Parasite Growth and Dihydroartemisinin Pharmacodynamics in Murine Malaria

2012 ◽  
Vol 57 (1) ◽  
pp. 508-516 ◽  
Author(s):  
Kashyap Patel ◽  
Kevin T. Batty ◽  
Brioni R. Moore ◽  
Peter L. Gibbons ◽  
Jürgen B. Bulitta ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Model Development ◽  
Treatment Strategies ◽  
Compartment Model ◽  
Content Type ◽  
Promising Tool ◽  
Efflux Pathway ◽  
Novel Model ◽  
Insight Into ◽  
Late Trophozoite

ABSTRACTMurine models are used to study erythrocytic stages of malaria infection, because parasite morphology and development are comparable to those in human malaria infections. Mechanism-based pharmacokinetic-pharmacodynamic (PK-PD) models for antimalarials are scarce, despite their potential to optimize antimalarial combination therapy. The aim of this study was to develop a mechanism-based growth model (MBGM) forPlasmodium bergheiand then characterize the parasiticidal effect of dihydroartemisinin (DHA) in murine malaria (MBGM-PK-PD). Stage-specific (ring, early trophozoite, late trophozoite, and schizont) parasite density data from Swiss mice inoculated withPlasmodium bergheiwere used for model development in S-ADAPT. A single dose of intraperitoneal DHA (10 to 100 mg/kg) or vehicle was administered 56 h postinoculation. The MBGM explicitly reflected all four erythrocytic stages of the 24-hourP. bergheilife cycle. Merozoite invasion of erythrocytes was described by a first-order process that declined with increasing parasitemia. An efflux pathway with subsequent return was additionally required to describe the schizont data, thus representing parasite sequestration or trapping in the microvasculature, with a return to circulation. A 1-compartment model with zero-order absorption described the PK of DHA, with an estimated clearance and distribution volume of 1.95 liters h−1and 0.851 liter, respectively. Parasite killing was described by a turnover model, with DHA inhibiting the production of physiological intermediates (IC50, 1.46 ng/ml). Overall, the MBGM-PK-PD described the rise in parasitemia, the nadir following DHA dosing, and subsequent parasite resurgence. This novel model is a promising tool for studying malaria infections, identifying the stage specificity of antimalarials, and providing insight into antimalarial treatment strategies.

scholarly journals Current perspective on production and applications of microbial cellulases: a review

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Nisha Bhardwaj ◽  
Bikash Kumar ◽  
Komal Agrawal ◽  
Pradeep Verma
Keyword(s):  
Critical Factor ◽  
Industrial Sector ◽  
Industrial Applications ◽  
Cellulolytic Enzymes ◽  
Promising Tool ◽  
The Status ◽  
Key Enzyme ◽  
Increasing Demand ◽  
Immobilization Techniques ◽  
Insight Into

AbstractThe potential of cellulolytic enzymes has been widely studied and explored for bioconversion processes and plays a key role in various industrial applications. Cellulase, a key enzyme for cellulose-rich waste feedstock-based biorefinery, has increasing demand in various industries, e.g., paper and pulp, juice clarification, etc. Also, there has been constant progress in developing new strategies to enhance its production, such as the application of waste feedstock as the substrate for the production of individual or enzyme cocktails, process parameters control, and genetic manipulations for enzyme production with enhanced yield, efficiency, and specificity. Further, an insight into immobilization techniques has also been presented for improved reusability of cellulase, a critical factor that controls the cost of the enzyme at an industrial scale. In addition, the review also gives an insight into the status of the significant application of cellulase in the industrial sector, with its techno-economic analysis for future applications. The present review gives a complete overview of current perspectives on the production of microbial cellulases as a promising tool to develop a sustainable and greener concept for industrial applications.

scholarly journals Blockchain for healthcare records: A data perspective

2018 ◽  
Author(s):  
Mian Zhang ◽  
Yuhong Ji
Keyword(s):  
Data Privacy ◽  
Healthcare Systems ◽  
Multiple Perspectives ◽  
Health Records ◽  
Healthcare Data ◽  
Blockchain Technology ◽  
Security Control ◽  
Patient Health ◽  
And Storage ◽  
Insight Into

A problem facing healthcare record systems throughout the world is how to share the medical data with more stakeholders for various purposes without sacrificing data privacy and integrity. Blockchain, operating in a state of consensus, is the underpinning technology that maintains the Bitcoin transaction ledger. Blockchain as a promising technology to manage the transactions has been gaining popularity in the domain of healthcare. Blockchain technology has the potential of securely, privately, and comprehensively manage patient health records. In this work, we discuss the latest status of blockchain technology and how it could solve the current issues in healthcare systems. We evaluate the blockchain technology from the multiple perspectives around healthcare data, including privacy, security, control, and storage. We review the current projects and researches of blockchain in the domain of healthcare records and provide the insight into the design and construction of next generations of blockchain-based healthcare systems.

scholarly journals SWOT analysis of wine tourism in Srem

Marketing ◽  
2020 ◽  
Vol 51 (4) ◽  
pp. 247-258
Author(s):  
Melita Josipović ◽  
Jelena Tepavčević ◽  
Svetlana Vukosav
Keyword(s):  
Swot Analysis ◽  
Wine Production ◽  
New Destinations ◽  
Wine Tourism ◽  
Travel Agencies ◽  
The World ◽  
Marketing Activities ◽  
And Storage ◽  
Insight Into ◽  
Growing Region

The importance of wine tourism is growing, both in the world and in our country. According to the number of registered wineries, the Srem wine-growing region is the largest in Serbia. Tourists traveling for wine tourism list wine tasting and introduction to the process of wine production, bottling and storage as their main motives. In addition to the above, wine lovers have the desire to spend quality time together in a natural environment. Marketing activities, which are undertaken for the promotion of wine tourism, are different. The promotion is performed by wineries, tourist organizations and travel agencies. In order to gain insight into the ways of promoting wine tourism in Srem, a short survey was conducted on two occasions, in 2013 and 2020. The survey was conducted in tourist organizations and tourist agencies, which operate in the territory of Srem. Potential tourists most often rely on the Internet, when choosing new destinations. Wine tourism research around the world has evolved from conceptual and case studies, to more comprehensive and extensive research (Carlsen & Charters, 2006). The aim of this paper is to present through SWOT analysis the strengths and opportunities that can contribute to the development of wine tourism in Srem, and on the other hand, identify weaknesses and threats. With the help of the TOWS matrix, strategies have been defined, the application of which could improve wine tourism in Srem.

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