scholarly journals Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study

Life ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1145
Author(s):  
Mayte Buchbender ◽  
Amelie Lugenbühl ◽  
Jakob Fehlhofer ◽  
Christian Kirschneck ◽  
Jutta Ries ◽  
...  
Keyword(s):  
Mouth Opening ◽  
Gingival Recession ◽  
Systemic Scleroderma ◽  
Clinical Parameters ◽  
Clinical Attachment Loss ◽  
Expression Levels ◽  
Periodontal Tissues ◽  
Healthy Control ◽  
Group 2 ◽  
Group 1

Background: Systemic scleroderma (SSc) has multiple orofacial effects. The aim of this study was to analyze the expression of inflammatory mediators in biofilm samples. It was hypothesized that different expression levels and clinical associations might be drawn. Methods: A total of 39 biofilm samples from group 1 = SSc and group 2 = healthy control were examined for the expression levels of interleukin (IL)-2,-6, and -10; matrix metalloprotease (MMP)-9; and surface antigens CD90 and CD34 by quantitative real-time PCR and clinical parameters. Relative quantitative (RQ) gene expression was determined using the ∆∆CT method. Results: The mean bleeding on probing values (p = 0.006), clinical attachment loss (CAL) (p = 0.009), gingival recession (p = 0.020), limited mouth opening (p = 0.001) and cervical tooth defects (p = 0.011) were significantly higher in group 1. RQ expressions of IL-2 and CD34 were significantly lower, IL-6, MMP-9, and CD90 were significantly higher. There was a significant positive correlation of IL-6/MMP-9 and negative correlation of mouth opening/CAL and IL-6/CAL. Conclusion: Different expression levels of IL-2, IL-6, MMP-9, CD34 and CD90 were detected in biofilm samples from patients with SSc compared to control. An immunological correlation to the clinical parameters of mouth opening and CAL was shown; thus, we conclude that SSc might have an impact on periodontal tissues.

scholarly journals Influence of Periodontal Disease on cardiovascular markers in Diabetes Mellitus patients

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Juliana de Fatima Pedroso ◽  
Zahra Lotfollahi ◽  
Ghadeer Albattarni ◽  
Maiara Arrruda Schulz ◽  
Andrea Monteiro ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Total Cholesterol ◽  
Periodontal Therapy ◽  
Optical Measurements ◽  
Diabetic Patients ◽  
Clinical Parameters ◽  
Periodontal Debridement ◽  
Group 2 ◽  
Hs Crp ◽  
Group 1

Abstract The objective of the present study was to establish if individuals with Diabetes Mellitus (DM2) and periodontal diseases (gingivitis or periodontitis) presented an increase in the concentration of modified LDL (moLDL) and what is the influence of periodontal treatment on the decrease of moLDL particles with consequent improvement in the parameters of DM2. Twenty-four diabetic patients with periodontitis (Group 1) and twenty-four diabetic patients with gingivitis (Group 2) were followed up for a period of 12 months. Group 1 was treated with periodontal debridement, and Group 2 received supra-gingival scaling and prophylaxis. In both groups, periodontal clinical parameters: probing depth (PD), clinical attachment level (CAL), gingival resection (GR), bleeding on probing index (BOP) and plaque index; inflammatory serum markers (glycemia, A1c, total cholesterol, HDL-cholesterol (HDL-c), LDL-cholesterol (LDL-c), triglycerides and hs-CRP) and oxidized LDL (oxLDL) were measured at baseline, t = 6 and t = 12 months after treatment. Solutions of LDL were analyzed using the nonlinear optical Z-Scan and optical absorption techniques. The periodontal clinical parameters showed significant improvement (p < 0.05) in both Group after 12 months. For both groups, total cholesterol, HDL-c, LDL-c, triglycerides and A1c levels did not show significant reductions after periodontal therapy. hs-CRP levels in Group 1 presented a significant reduction after 12 months. The glycemic rate and the oxLDL concentrations did not show significant differences as a function of time. The optical measurements of LDL solutions revealed an improvement of the LDL-c quality in both groups. Periodontal debridement was able to improve periodontal parameters and the quality of LDL-c in diabetic patients but without changes in the oxLDL concentration in both groups. Considering the clinical relevance, the reduction of infectious and inflammatory sites present in the oral cavity through periodontal therapy may help with the control and prevention of hyperglycemia and precursors of cardiovascular diseases.

scholarly journals EVALUATION OF PATIENT'S PERCEPTION AND HEALING, FOLLOWING CONVENTIONAL AND PARALLELING TECHNIQUE OF FRENECTOMY USING SCALPEL AND OTHER MODALITY

2020 ◽  
Vol 6 (1) ◽  
pp. 21-27
Author(s):  
Dr. Ira Gupta ◽  
Saranik Sarkar ◽  
Rohit Gupta ◽  
Nidhi Gupta ◽  
Janardhana Amaranath B. J. ◽  
...  
Keyword(s):  
Wound Healing ◽  
Gingival Recession ◽  
Conventional Technique ◽  
Treatment Modalities ◽  
Group 3 ◽  
Group 2 ◽  
Patient’S Perception ◽  
Better Than ◽  
Group 1 ◽  
Operative Wound

Background: Frenum that encroaches on the margin of the gingiva may interfere with plaque removal and can lead to gingival recession and midline diastema. Thus, to maintain proper oral hygiene and prevent gingival recession, frenectomy is done. Frenectomy can be accomplished by conventional frenectomy technique, which has certain disadvantages. To overcome those drawbacks, paralleling technique of frenectomy has been introduced. Both the techniques can be performed by using various treatment modalities. Aim: The purpose of this clinical study was to compare the bleeding during surgery, post-operative wound healing and patient's perception towards the conventional frenectomy technique with scalpel, paralleling frenectomy technique with scalpel and paralleling technique with electrocautery. Materials and method: A total of 30 subjects with gingival or papillary frenal attachment were selected and equally divided into three groups. Group 1 was treated by conventional technique with scalpel, Group 2 by paralleling technique with scalpel and Group 3 by paralleling technique with electrocautery. Various parameters such as bleeding during surgery, patient's perception towards the various techniques and post-operative wound healing were evaluated. Results: The results showed that bleeding during surgery was minimum in group 3 as compared to other two groups. Patient's perception towards paralleling technique with scalpel, was found better than the other groups. At 7th day, though there was a difference in the healing but at 21st day wound healing was almost similar in all the three groups. Conclusion: Based on our findings and clinical outcome, paralleling technique with scalpel provided better patient's perception and an efficient and satisfactory option for frenectomy.  

scholarly journals Evaluation of Serum Resistin Levels in Periodontal Health and Disease and Effects of Non Surgical Periodontal Therapy on Its Levels

2012 ◽  
Vol 32 (5) ◽  
pp. 289-294 ◽  
Author(s):  
Archana Devanoorkar ◽  
C.D. Dwarakanath ◽  
Gayatri Gundanavar ◽  
Rahul Kathariya ◽  
Sudhir R Patil
Keyword(s):  
Statistical Significance ◽  
Periodontal Therapy ◽  
Control Group ◽  
Periodontal Health ◽  
Pocket Depth ◽  
Clinical Attachment Loss ◽  
Nonsurgical Periodontal Therapy ◽  
Health And Disease ◽  
Group 2 ◽  
Group 1

Background: Resistin and adiponectin are the adipokines secreted by adipocytes and various inflammatory cells. These adipokines are known to play an important role in insulin resistance. The aim of this study was to determine the serum resistin levels in periodontal health and disease and also, to determine the effect of nonsurgical periodontal therapy on its levels.Methods: A total of 40 patients (20 Males and 20 Females; age range 20–50 years) participated in the study. Subjects were categorized as healthy (group 1; Controls) and chronic periodontitis (group 2; Study) groups based on their periodontal status. Periodontal parameters (Plaque index (PI), Gingival index (GI), Bleeding index (BI), Probing pocket depth (PPD), Clinical attachment loss (CAL)) together with serum resistin levels were assessed at baseline and between 6–8 weeks following nonsurgical periodontal therapy for subjects in group~2 and only at baseline in group 1. Sera were tested in duplicate (single run), and the results were averaged.Results: Study group showed higher (1.89 ± 1.83 ng/ml) serum resistin levels, compared to control group (1.35 ± 0.70 ng/ml). However, this difference was not statistically significant (P=0.227). Also, resistin levels decreased following nonsurgical periodontal therapy but, this decrease failed to show any statistical significance, with pretreatment levels being 1.89 ± 1.83 ng/ml and post treatment levels being 1.59 ± 1.01 ng/ml (P=0.386).Conclusion: Observations of the present study revealed that there was not much difference in the serum resistin levels between the cases and the controls. Also the decrease in the resistin levels following nonsurgical periodontal therapy did not show any statistical significance.

scholarly journals Efficacy of Different Concentrations of Chlorhexidine Mouthwash on Plaque Accumulation and Periodontal Parameters

2016 ◽  
Vol 8 (1) ◽  
pp. 8-11
Author(s):  
Ali Zarandi ◽  
Sina Salahaddin ◽  
Masoumeh Faramarzi
Keyword(s):  
Statistical Significance ◽  
Clinical Parameters ◽  
Chemical Agents ◽  
Probing Depth ◽  
Before And After ◽  
Chi Squared ◽  
Plaque Control ◽  
Group 2 ◽  
Antibacterial Potential ◽  
Group 1

Background and aims. Dental plaque and gingivitis were controlled by administration of chemical agents such as chlorhexidine (CHX) which is recognized as a gold standard of chemical agents. The aim of this study was to compare the effects of two mouthwashes (0.2% CHX and Kin Gingival) on clinical parameters. Materials and methods. A total of 88 subjects were included in this interventional‒experimental study. The subjects were divided into two groups of 44 (group 1: 0.2% CHX and group 2: Kin Gingival). The study involved no mechanical plaque control methods. Patients used the mouthwashes twice a day for two weeks. Clinical parameters included plaque index (PI), gingival index (GI), probing depth (PD) and bleeding on probing (BOP), which were measured before and after the use of mouthwashes. The results were analyzed by Man-Whitney U and chi-squared tests. Statistical significance was set at P < 0.05. Results. The results indicated that PI, GI and PD significantly decreased in group 2 (Kin Gingival) in comparison with group 1 (0.2% CHX) (P < 0.05). However, the two mouthwashes did not differ significantly from each other in relation to BOP (P > 0.05). Conclusion. Based on the results it can be concluded that Kin Gingival and 0.2% CHX mouthwashes decrease the clinical parameters in patients significantly. However, Kin Gingival is more effective than 0.2% CHX, which might be attributed to the synergic antibacterial potential of Kin Gingival ingredients like sodium fluoride.

scholarly journals CONDYLAR FRACTURE;

2019 ◽  
Vol 26 (03) ◽  
Author(s):  
Usman Ashraf ◽  
Shaheen Ahmed ◽  
Abdul Hafeez Shaikh ◽  
Noureen Iqbal ◽  
Syed Jaffar Abbas Zaidi ◽  
...  
Keyword(s):  
Treatment Outcomes ◽  
Single Case ◽  
Maxillofacial Surgery ◽  
Mouth Opening ◽  
Health Sciences ◽  
Oral And Maxillofacial Surgery ◽  
Plate Bending ◽  
Condylar Fracture ◽  
Group 2 ◽  
Group 1

Background: This study was conducted tocompare treatment outcomes of condylar fracture fixation usingeither single or double non-compressive mini plates. Study Design: Interventional study. Setting:  Department of Oral and Maxillofacial Surgery, Dr. IshratulIbad Khan Institute of Oral Health Sciences, Dow University of Health Sciences. Period: July 2017 till December 2017. Materials and Methods: A total of 30 participants were selected and randomly divided into 2 groups, each consisting of 15 participants.Patients treated with a single non-compression miniplate were placed in group 1, while those patients whose fractures were reduced and fixed by double non-compression mini plates were placed in group 2. Outcomes assessed were intraoperative time, deviation on mouth opening, malocclusion, plate bending, function of facial muscles and the relative cost of implants. Results: Out of a total of thirty patients, it was observed that those patients who were randomly assigned group 2 had better treatment outcomes and less complications as compared to the other group. Three patients with malocclusion were seen in group 1 compared with only a single case seen in group 2. Greater number of plate bending with susceptibility to plate fracture was also observed in Group 1. Conclusion: It can thus be concluded that using two plates for condylar fracture represents a more stable and sustainable solution with less complications in providing osteosynthesis.

scholarly journals Effect of Diode Laser on Gingival Crevicular Fluid Stromal Derived Factor – 1 Alpha Level in Chronic Periodontitis Patients- A Randomized Clinical Trial

2019 ◽  
Vol 1 (5) ◽  
Author(s):  
Rakesh M.P. ◽  
Krishna Kripal ◽  
Deepthi R. ◽  
Sandeep S. Prabhu ◽  
Shanmugapriya P.A.
Keyword(s):  
Periodontal Disease ◽  
Diode Laser ◽  
Gingival Crevicular Fluid ◽  
Immune Defense ◽  
Clinical Parameters ◽  
Scaling And Root Planing ◽  
Root Planing ◽  
Group 2 ◽  
Crevicular Fluid ◽  
Group 1

Background: SDF-1α may be involved in the immune defense pathway activated during periodontal disease. Upon development of disease, SDF-1α levels increase and may recruit host defensive cells into sites of inflammation. This suggests that SDF-1α may be a useful biomarker for the identification of periodontal disease progression. Previous studies suggested that diode laser using as an adjunct to SRP in clinical and biochemical benefits. Aims and objectives: The aim of the present study was to assess the effect of diode laser on gingival crevicular fluid stromal derived factor – 1 alpha (SDF-1α) level in chronic generalized periodontitis patients.  Materials and methods: A total of 30 patients were divided in two groups based on inclusion and exclusion criteria as follows: Group 1:  15 chronic generalized periodontitis patients who received treatment with scaling and root planing. Group 2: 15 chronic generalized periodontitis patients who received treatment with scaling and root planing and diode laser. Each GCF samples were collected at baseline, 15 days and 21 days after treatment. Clinical parameters like gingival index, probing pocket depth and clinical attachment level were recorded and GCF samples also collected. Results: All the clinical parameters (GI, PPD, CAL) and the concentration of SDF-1α level in GCF were found to be significantly reduced in the group 2 compared to group 1 after 21 days. The mean values of GI, PPD, CAL parameters at different time intervals was found statistically significant in both the groups (p<0.001). Conclusion: Within the limitations of the present study, we conclude that diode laser used as an adjunct to SRP provides greater improvement in clinical and biochemical benefits over conventional mechanical treatment during medium term of observation.

A COMPARATIVE STUDY ON RISK OF OSTEOPOROSIS ASSOCIATED WITH THE USE OF VALPROATE VERSUS LEVETIRACETAM IN EPILEPTIC PATIENTS AND ITS RELATIONSHIP WITH METHYLENE TETRA-HYDRO FOLATE REDUCTASE GENOTYPES

2021 ◽  
pp. 78-82
Author(s):  
MOHAMMED FAREEDULLAH ◽  
REHAB RAFI ◽  
NEHA NAAZ ◽  
MOHAMMED BARKATH ALI SHAMS ◽  
SYEDA SHAISTA BABAR ◽  
...  
Keyword(s):  
Mthfr Gene ◽  
Mineral Density ◽  
Mthfr Polymorphism ◽  
Recommended Treatment ◽  
Epileptic Patients ◽  
Healthy Control ◽  
Group 3 ◽  
Clonic Seizures ◽  
Group 2 ◽  
Group 1

Objective: The study objects at assessing and comparing the intensity of the effect of valproate (VPA) and levetiracetam (LV) on the bone mass in young adult epileptic patients while distinguishing their methylene tetra-hydro folate reductase (MTHFR) genotypes and correlating MTHFR polymorphism and antiepileptic drugs (AEDs) usage with the risk of development of osteoporosis. Methods: The study design was a comparative, prospective, and observational study. It was conducted at Princess Esra Hospital (PEH), Hyderabad and genotype testing was carried out at Salar-e-Millat Research lab (PEH). The consent was obtained from total 70 subjects, divided into three groups: Group 1: 18 patients receiving sodium VPA monotherapy Group 2: 17 patients receiving LV monotherapy Group 3: 35 healthy control subjects from general population. Patients of either gender within age group of 15–40 years, experiencing generalized tonic-clonic seizures or focal seizures, receiving the AED for duration of time ≥2 years were included in the study. Results: Our study showed significant correlation between the AEDs treatment and MTHFR polymorphism in predisposing osteoporosis. Conclusion: The variants of MTHFR gene (C677T) are prone to develop increased levels of homocysteine as a result of decreased activity of the enzyme in their bodies which are further increased in patients receiving AEDs. Monitoring of homocysteine levels in epileptic patients especially in the mutants of MTHFR gene along with their periodic testing of bone mineral density levels is recommended. Treatment for low folate and calcium levels is recommended in these patients to correct their deficiencies.

Comparative Evaluation to Assess the Effect of SRP With or Without Human Placental Extracts as Local Drug Delivery in Treatment of Localized Periodontal Pocket- A Randomized Controlled Clinical Trial

2020 ◽  
Vol 5 (2) ◽  
pp. 66-70
Author(s):  
Aakriti Sharma ◽  
◽  
Sanjeev Sharma ◽  
Amit Nagar ◽  
◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Local Drug Delivery ◽  
Periodontal Pocket ◽  
Controlled Clinical Trial ◽  
Clinical Parameters ◽  
Novel Therapy ◽  
Gelatin Matrix ◽  
Etiologic Agents ◽  
Group 2 ◽  
Group 1

Background: Periodontal disease is one of the most prevalent global chronic disorder. Pathology affecting the structure surrounding teeth results in inflammation initiated by bacterial aggregation & alteration in their profile. Conventional periodontal therapeutics has focused on the control of etiologic agents, thereby promoting healing & repair of tissues. Delivery of therapeutic agents into the local milieu act as drug reservoirs which could alter pathogenic flora & promote its repair & wound healing. Aim & Objective: In an effort to develop a novel therapy, the present study aims to compare clinical soft tissue parameters around periodontal pockets treated with & without human placental extracts delivered locally. Materials & Method: In 10 patients of chronic periodontitis, bilateral localized periodontal pockets of 4-6 mm depth were included. Each site was randomly allocated to group 1 & group 2,where in group 1, scaling & root planing( SRP) was done while in group 2,Placentrex Gel (Human placental extracts) adsorbed on absorbable gelatin matrix (Abgel) used as a vehicle was delivered as an adjunct to SRP . Clinical parameters were recorded & re- evaluated after one month for both the treatment groups . Statistical analysis was carried out to evaluate the effect of Placentrex gel as an adjunct to SRP in treatment of periodontal pockets, Mann Whitney & Paired t test were done. Results: There was a statistically significant improvement in clinical parameters with notable difference in probing depth reduction & gain of clinical attachment level in the treatment group subjected to SRP & Placental extract delivery. Conclusion: Clinical variables showed a greater efficacy of SRP & adjunctive use of placental extracts as compared to conventional treatment by SRP thereby underlying placental extracts usage in the management of periodontal disease condition

Molecular regulation of polycystic ovary syndrome: altered gene expression levels in mouse models pretreatment and post-treatment

Zygote ◽  
2021 ◽  
pp. 1-6
Author(s):  
P. Tulay ◽  
T. Onal ◽  
S. Vatansever
Keyword(s):  
Gene Expression ◽  
Polycystic Ovary Syndrome ◽  
Mouse Models ◽  
Polycystic Ovary ◽  
Clomiphene Citrate ◽  
Ovary Syndrome ◽  
Expression Levels ◽  
Group 2 ◽  
Gene Expression Levels ◽  
Group 1

Summary Polycystic ovary syndrome (PCOS) is a complex disorder and genetic factors are believed to play a role. The main aim was to investigate expression levels of genes involved in PI3K/AKT signalling pathway pretreatment and post-treatment. Mouse models of PCOS were generated. Group one included control mice with no polycystic ovaries (n = 4), Group 2 included a PCOS mouse model (n = 8), Group 3 included PCOS mice treated with clomiphene citrate (n = 7) and Group 4 included PCOS mice treated with clomiphene citrate, metformin and pioglitazone (n = 8). Histochemical analyses were performed. Total RNA was extracted and cDNA was synthesized. Irs, Akt1 and Akt2, mTor and Pdpk1 gene expression levels were evaluated by RT-PCR amplification. In Group 1, cortex and medulla were evaluated as normal; in Group 2, ovarian cortex was composed of immature oocytes and cystic follicles with atretic follicles. In Groups 3 and 4, follicles were in the process of normal follicle differentiation. The expression levels of Akt1 and Pi3k were significantly different (P < 0.0001) between Groups 1 and 2. The significant differences in expression levels of Pi3k and Akt1 were also observed between the Group 1 and both Groups 3 and 4 (P < 0.0001). Furthermore, significant variations of the expression levels of mTor between Groups 1 and 4 were observed. The extrapolation of results of this study may imply that follicular development may be regulated by molecular pathways involving Pi3k, Akt1 and mTor expression. Therefore, genes in the PI3K/AKT pathway may have a direct regulatory role in the development of PCOS.

Expression Levels of A Disintegrin-like Metalloproteinase with Thrombospondin Motifs-4 and -5 (ADAMTS-4 and ADAMTS-5) in Inflamed and Healthy Gingival Tissues

2020 ◽  
Vol 23 (2) ◽  
pp. 168-176
Author(s):  
Mahmure A. Tayman ◽  
İsmail Koyuncu ◽  
Nimet Ö. Köklü
Keyword(s):  
Stage Iii ◽  
Expression Levels ◽  
Positive Correlation ◽  
Protein Levels ◽  
Periodontal Tissues ◽  
Physiological Processes ◽  
Healthy Control ◽  
Ecm Degradation ◽  
Periodontal Destruction ◽  
Clinical Measurements

Background: ADAMTS (A disintegrin-like metalloproteinase with thrombospondin motifs) is a group of 19 zinc-dependent metalloproteases known to function in many pathological and physiological processes, such as adhesion, cell fusion, signaling, proteolysis and ECM degradation. Objectives: The aim of this study was to demonstrate the levels of ADAMTS-4 and -5 in gingival tissues with Stage III-Grade B generalized periodontitis (SIII-GB), Stage III-Grade C generalized periodontitis (SIII-GC) and healthy-control (C) groups. Methods: The clinical measurements were recorded for each patient. A total of 63 gingival biopsy specimens were obtained from the C (n:20), SIII-GB (n:23) and SIII-GC (n:20) groups. Polymerase chain reaction (Rt-PCR) and immunohistochemical (IHC) examinations were used to determine gene and protein levels. Results: According to the results of all methods, ADAMTS-4 and -5 expressions existed in periodontitis and C groups (P> 0.05). Immunostaining for ADAMTS-4 was found to be higher in patients with periodontitis than for ADAMTS-5 (P>0.05). Gene expression levels for ADAMTS-4 and -5 seemed to be up-regulated in subjects diagnosed with periodontitis, but the results were not statistically significant (P>0.05). A positive correlation was observed between PPD and ADAMTS-4 mRNA in SIII-GC (p=0.035) and SIII-GB (p=0.015). A positive correlation was determined between ADAMTS-4 mRNA and ADAMTS-5 mRNA in SIII-GC (p=0.037) and SIII-GB (p=0.00). Conclusion: ADAMTS expression may take part in both pathological and physiological processes in the periodontal tissues, and periodontal destruction may be the result of a complex interaction of several pathways with many participants, such as ADAMTS-4 and -5, thus facilitating the exaggeration of periodontal disease.

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