scholarly journals Effect of Different Additives in Diets on Secondary Structure, Thermal and Mechanical Properties of Silkworm Silk

Materials ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 14 ◽  
Author(s):  
Lan Cheng ◽  
Huiming Huang ◽  
Jingyou Zeng ◽  
Zulan Liu ◽  
Xiaoling Tong ◽  
...  

In this study, eight types of materials including nanoparticles (Cu and CaCO3), metallic ions (Ca2+ and Cu2+), and amino acid substances (serine, tyrosine, sericin amino acid, and fibroin amino acid) were used as additives in silkworm diets to obtain in-situ modified silk fiber composites. The results indicate that tyrosine and fibroin amino acids significantly increase potassium content in silk fibers and induce the transformation of α-helices and random coils to β-sheet structures, resulting in higher crystallinities and better mechanical properties. However, the other additives-modified silk fibers show a decrease in β-sheet contents and a slight increase or even decrease in tensile strengths. This finding provides a green and effective approach to produce mechanically enhanced silk fibers with high crystallinity on a large scale. Moreover, the modification mechanisms of these additives were discussed in this study, which could offer new insights into the design and regulation of modified fibers or composites with desirable properties and functions.

2021 ◽  
Vol 22 (4) ◽  
pp. 1513
Author(s):  
Shuo Zhao ◽  
Xiaogang Ye ◽  
Meiyu Wu ◽  
Jinghua Ruan ◽  
Xiaoxiao Wang ◽  
...  

This paper explores the structures of exogenous protein molecules that can effectively improve the mechanical properties of silkworm silk. Several transgenic vectors fused with the silkworm fibroin light chain and type 3 repeats in different multiples of the ampullate dragline silk protein 1 (MaSp1) from black widow spider with different lengths of the polyalanine motifs were constructed for this study. Transgenic silkworms were successfully obtained by piggyBac-mediated microinjection. Molecular detection showed that foreign proteins were successfully secreted and contained within the cocoon shells. According to the prediction of PONDR® VSL2 and PONDR® VL-XT, the type 3 repeats and the polyalanine motif of the MaSp1 protein were amorphous. The results of FTIR analysis showed that the content of β-sheets in the silk of transgenic silkworms engineered with transgenic vectors with additional polyalanine was significantly higher than that of wild-type silkworm silk. Additionally, silk with a higher β-sheet content had better fracture strength and Young’s modulus. The mechanical properties of silk with longer chains of exogenous proteins were improved. In general, our results provide theoretical guidance and technical support for the large-scale production of excellent bionic silk.


Soft Matter ◽  
2007 ◽  
Vol 3 (7) ◽  
pp. 877-882 ◽  
Author(s):  
Lawrence F. Drummy ◽  
B. L. Farmer ◽  
Rajesh R. Naik

2020 ◽  
Author(s):  
Alessandro Frigeri ◽  
Maria Cristina De Sanctis ◽  
Francesca Altieri ◽  
Simone De Angelis ◽  
Marco Ferrari ◽  
...  

<p>The ExoMars Rover and Surface Platform planned for launch in 2022 is a large international cooperation between the European Space Agency and Roscosmos with a scientific contribution from NASA.  Thales Alenia Space is the ExoMars mission industrial prime contractor. </p> <p>Besides sensors and instruments characterizing the surface at large scale, the ExoMars’ rover Rosalind Franklin payload features some experiments devoted specifically to the characterization of the first few meters of the Martian subsurface. These experiments are particularly critical for the main ExoMars objective of detecting traces of present or past life forms on Mars, which may have been preserved within the shallow Martian underground [1].</p> <p>Rosalind Franklin will be able to perform both non-invasive geophysical imaging of the underground [2] and subsurface <em>in situ</em> measurements thanks to the Drill unit installed on the rover. The Drill has been developed by Leonardo and its purposes are 1) to collect core samples to be analyzed in the Analytical Laboratory Drawer (ALD) onboard the Rover and 2) to drive the miniaturized spectrometer Ma_MISS within the borehole.   </p> <p>Ma_MISS (Mars Multispectral Imager for Subsurface Studies, [3]) will collect mineralogic measurements from the rocks exposed into the borehole created by the Drill with a spatial resolution of 120 μm down to 2 meters into the Martian subsurface.</p> <p>Rocks are composed of grains of minerals, and their reaction to an applied stress is related to the mechanical behavior of the minerals that compose the rock itself. The mechanical properties of a mineral depend mainly on the strength of the chemical bonds, the orientation of crystals, and the number of impurities in the crystal lattice.</p> <p>In this context, the integration of Ma_MISS measurements and drill telemetry are of great importance.  The mechanical properties of rocks coupled with their mineralogic composition provide a rich source of information to characterize the nature of rocks being explored by ExoMars rover’s drilling activity.</p> <p>Within our study, we are starting to collect telemetry recorded during the Drill unit tests on several samples ranging from sedimentary to volcanic rocks with varying degrees of weathering and water content.  In this first phase of the study, we focused our attention on the variation of torque and penetration speed between different samples, which have been found to be indicative of a particular type of rock or group of rocks and their water content.  </p> <p>We are planning to analyze the same rocks with the Ma_MISS breadboard creating the link between the mineralogy and the mechanical response of the Drill.      </p> <p>This will put the base for a more comprehensive and rich characterization of the <em>in situ</em> subsurface observation by Rosalind Franklin planned at Oxia Planum, Mars in 2023. </p> <p> </p> <p><strong>Acknowledgments: </strong>We thank the European Space Agency (ESA) for developing the ExoMars Project, ROSCOSMOS and Thales Alenia Space for rover development, and Italian Space Agency (ASI) for funding the Ma_MISS experiment (ASI-INAF contract n.2017-48-H.0 for ExoMars MA_MISS phase E/science).</p> <p> </p> <p><strong>References</strong></p> <p>[1] Vago et al., 2017. Astrobiology, 17 6-7. [2] Ciarletti et al., 2017. Astrobiology, 17 6-7. [3] De Sanctis et al., 2017. Astrobiology, 17 6-7.</p>


2011 ◽  
Vol 175-176 ◽  
pp. 95-99 ◽  
Author(s):  
Hui Jing Zhao ◽  
Ming Zhong Li

Silkworm silk has been recognized as a satisfactory biomaterial for long time due to its exceptional biocompatibility, biodegradability, mechanical properties etc. For example, silk fibers in the form of sutures have been used for centuries. The aim of this study is to discuss the potential usage of silk as the novel biomedical devices, such as blood vessels. In this study, cuit silks prepared from degummed raw silks were twisted as threads with four different yarn linear densities. A specific braiding machine was used to weave those threads into a tube. Subsequently two different groups of silk tubes were prepared. One was treated by ethanol and the other without. Thickness, porosity, mass per unit area of two groups of braided tubes were measured. Its mechanical properties were also studied. The influence of ethanol treatment and various yarn linear densities on its structural and mechanical properties was also studied. Results indicated that structural and mechanical properties of the tubes were significatly changed by the yarn linear densities and ethanol treatment. Conclusively, braided silk tube could be a potential blood vessel tissue engineering scaffold.


2012 ◽  
Vol 9 (75) ◽  
pp. 2479-2487 ◽  
Author(s):  
Sean J. Blamires ◽  
Chung-Lin Wu ◽  
Todd A. Blackledge ◽  
I-Min Tso

Phenotypic variation facilitates adaptations to novel environments. Silk is an example of a highly variable biomaterial. The two-spidroin (MaSp) model suggests that spider major ampullate (MA) silk is composed of two proteins—MaSp1 predominately contains alanine and glycine and forms strength enhancing β-sheet crystals, while MaSp2 contains proline and forms elastic spirals. Nonetheless, mechanical properties can vary in spider silks without congruent amino acid compositional changes. We predicted that post-secretion processing causes variation in the mechanical performance of wild MA silk independent of protein composition or spinning speed across 10 species of spider. We used supercontraction to remove post-secretion effects and compared the mechanics of silk in this ‘ground state’ with wild native silks. Native silk mechanics varied less among species compared with ‘ground state’ silks. Variability in the mechanics of ‘ground state’ silks was associated with proline composition. However, variability in native silks did not. We attribute interspecific similarities in the mechanical properties of native silks, regardless of amino acid compositions, to glandular processes altering molecular alignment of the proteins prior to extrusion. Such post-secretion processing may enable MA silk to maintain functionality across environments, facilitating its function as a component of an insect-catching web.


Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1013 ◽  
Author(s):  
Hsuan-Chen Wu ◽  
Aditi Pandey ◽  
Liang-Yu Chang ◽  
Chieh-Yun Hsu ◽  
Thomas Chung-Kuang Yang ◽  
...  

The superlative mechanical properties of spider silk and its conspicuous variations have instigated significant interest over the past few years. However, current attempts to synthetically spin spider silk fibers often yield an inferior physical performance, owing to the improper molecular interactions of silk proteins. Considering this, herein, a post-treatment process to reorganize molecular structures and improve the physical strength of spider silk is reported. The major ampullate dragline silk from Nephila pilipes with a high β-sheet content and an adequate tensile strength was utilized as the study material, while that from Cyrtophora moluccensis was regarded as a reference. Our results indicated that the hydrothermal post-treatment (50–70 °C) of natural spider silk could effectively induce the alternation of secondary structures (random coil to β-sheet) and increase the overall tensile strength of the silk. Such advantageous post-treatment strategy when applied to regenerated spider silk also leads to an increment in the strength by ~2.5–3.0 folds, recapitulating ~90% of the strength of native spider silk. Overall, this study provides a facile and effective post-spinning means for enhancing the molecular structures and mechanical properties of as-spun silk threads, both natural and regenerated.


RSC Advances ◽  
2014 ◽  
Vol 4 (27) ◽  
pp. 14304-14313 ◽  
Author(s):  
Lin Liu ◽  
Xiaogang Yang ◽  
Houyong Yu ◽  
Chao Ma ◽  
Juming Yao

Biomimic silk fibers with refined crystalline structure were produced via incorporating cellulose nanocrystals into silk fibroin matrix to mimic the β-sheet crystallites in natural silk. The fibers exhibit excellent thermal and mechanical properties, attributed to the strong hydrogen bonding interactions between cellulose nanocrystals and silk fibroin as well as cellulose nanocrystal-induced ordered structure.


2016 ◽  
Vol 29 (7) ◽  
pp. 808-815 ◽  
Author(s):  
Bo Gao ◽  
Wentao Du ◽  
Qinghai Ma ◽  
Ruliang Zhang ◽  
Chengguo Wang ◽  
...  

Low-generation poly(amido amine) (PAMAM)-grafted carbon fibers (CFs) emerged as a new reinforcement for improving the mechanical properties of fiber composites. In this work, hybrid reinforcement, which could greatly enhance the surface roughness and wettability of CF, was prepared via growing PAMAM onto fiber surface by in situ polymerization.The modified surface morphology and chemical composition were investigated by scanning electron microscopy, atomic force microscopy, dynamic contact angle analysis test, and X-ray photoelectron spectroscopy. Experimental results indicated PAMAM dendrimers grown on the CF significantly enhanced interfacial properties of the resulting composites. In addition, compared with the desized CF composites, the CF grafted with PAMAM composites exhibited 34.65% enhancement in the interfacial shear strength.


1989 ◽  
Vol 171 ◽  
Author(s):  
N. Rungsimuntakul ◽  
S.V. Lonikar ◽  
R. E. Fornes ◽  
R.D. Gilbert

ABSTRACTThe mechanical properties of epoxy resins and epoxy resin/graphite fiber composites are adversely affected by moisture absorption. Incorporation of masked isocyanates that unmask to release isocyanates in situ at the cure temperatures (150-177ºC) reduce the equilibrium absorption up to ∼70%. Dynamic mechanical analyses and stress-strain properties of epoxy resins containing masked isocyanates were examined to determine their effect on mechanical properties. The ultimate Tg of the epoxy is reduced by incorporation of masked isocyanate, but the actual Tg is comparable to the “as cured” Tg of the epoxy. The dynamic moduli up to the Tg are relatively unaffected. Ina number of cases, the initial modulus, elongation at break and peak stress are equal or better than the unmodified resins.


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