The Technological Process of Obtaining New Linen Dressings Did Not Cause the Loss of Their Wound-Healing Properties

Background: Linen dressings were invented a few years ago but are still being worked on. Methods: The obtained fabrics from the traditional variety of flax (Nike), two transgenic types of flax (M50 and B14) and the combination of these two flax fibers (M50 + B14) were tested in direct contact in cell cultures. Cell viability tests were performed, and the proliferation potential of cells on Balb3T3 and NHEK cell lines was checked using the Sulforhodamine-B (SRB) test. Moreover, the effect of new linen fabrics on apoptosis of THP-1 cells, as well as on the cell cycle of NHEK, HMCEV and THP-1, cells after 24 h of incubation was assessed. Results: All tested linen fabrics did not raise the number of necrotic cells. The tested fabrics caused a statistically significant decrease in the total protein content in skin cancer (except for 0.5 cm of Nike-type fabrics). The smallest cells in the apoptotic phase were in cultures treated with M50 fiber on an area of 0.5 cm. After 48 h of incubation of HEMVEC, NHEK and THP-1 cells with the tested fabrics, the growth of S-phase cells was noticed in all cases. At the same time, the greatest increase was observed with the use of B14 fabric. Necrosis is not statistically significant. Conclusions: All the obtained flax fibers in the form of flax dressings did not lose their wound-healing properties under the influence of the technological process. New dressings made of genetically modified flax are a chance to increase the effectiveness of treatment of difficult healing wounds.

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Cutaneous wound healing is impaired in hemophilia B

Blood ◽

10.1182/blood-2006-05-020495 ◽

2006 ◽

Vol 108 (9) ◽

pp. 3053-3060 ◽

Cited By ~ 74

Author(s):

Maureane Hoffman ◽

Anna Harger ◽

Angela Lenkowski ◽

Ulla Hedner ◽

Harold R. Roberts ◽

...

Keyword(s):

Wound Healing ◽

Time Course ◽

Ferric Iron ◽

Hemophilia B ◽

Cutaneous Wound Healing ◽

Delayed Wound Healing ◽

Cutaneous Wound ◽

Time Points ◽

Hemophilic Arthropathy ◽

Healing Wounds

Abstract We used a mouse model to test the hypothesis that the time course and histology of wound healing is altered in hemophilia B. Punch biopsies (3 mm) were placed in the skin of normal mice and mice with hemophilia. The size of the wounds was measured daily until the epidermal defect closed. All wounds closed in mice with hemophilia by 12 days, compared with 10 days in normal animals. Skin from the area of the wound was harvested at different time points and examined histologically. Hemophilic animals developed subcutaneous hematomas; normal animals did not. Macrophage infiltration was significantly delayed in hemophilia B. Unexpectedly, hemophilic mice developed twice as many blood vessels in the healing wounds as controls, and the increased vascularity persisted for at least 2 weeks. The deposition and persistence of ferric iron was also greater in hemophilic mice. We hypothesize that iron plays a role in promoting excess angiogenesis after wounding as it had been proposed to do in hemophilic arthropathy. We have demonstrated that impaired coagulation leads to delayed wound healing with abnormal histology. Our findings have significant implications for treatment of patients with hemophilia, and also highlight the importance of rapidly establishing hemostasis following trauma or surgery.

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The Regenerative Potential of Substance P

International Journal of Molecular Sciences ◽

10.3390/ijms23020750 ◽

2022 ◽

Vol 23 (2) ◽

pp. 750

Author(s):

Patrycja Redkiewicz

Keyword(s):

Wound Healing ◽

Substance P ◽

Autoimmune Diseases ◽

Chronic Wounds ◽

Different Types ◽

Healing Wounds ◽

Active Substances

Wound healing is a highly coordinated process which leads to the repair and regeneration of damaged tissue. Still, numerous diseases such as diabetes, venous insufficiencies or autoimmune diseases could disturb proper wound healing and lead to chronic and non-healing wounds, which are still a great challenge for medicine. For many years, research has been carried out on finding new therapeutics which improve the healing of chronic wounds. One of the most extensively studied active substances that has been widely tested in the treatment of different types of wounds was Substance P (SP). SP is one of the main neuropeptides released by nervous fibers in responses to injury. This review provides a thorough overview of the application of SP in different types of wound models and assesses its efficacy in wound healing.

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Novel formulation approaches for wound healing

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.4227 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 937-948

Author(s):

Tanaji D Nandgude ◽

Syed Nateque Naser

Keyword(s):

Wound Healing ◽

Healing Process ◽

Cell Types ◽

Wound Management ◽

Wound Healing Process ◽

Anatomic Structure ◽

Tissue Integrity ◽

Molecular Events ◽

Acute Injuries ◽

Healing Wounds

A wound is damage to the typical anatomic structure. Wound healing is an immediate therapeutic response to injury. It is a creation of the combined response of some cell types towards injury. Wound healing takes place by a sequence of molecular events which cooperate to fix tissue integrity and cell work. In typical healthy individual under ordinary conditions, these physiological events take place smoothly. Though sometimes, these molecular events are arrested, this brings about in struggle to heal. There is an assortment of approaches for the way toward managing and controlling both acute injuries (acute wounds) and ceaseless non-mending wounds (chronic non-healing wounds). The principal objective of these two cases is to achieve better-wound healing. Ideal formulations of wound healing should not only enhance the healing process but also reduce pain, infection and loss of electrolytes, proteins and liquids from the injury. A broad scope of items typically introduced with target various parts of the wound healing process depends on numerous types of wounds and novel polymers utilised for the conveyance of medications to both acute and ceaseless injuries. These include alginate, hydrocolloids, hydrofibers, polyurethane, and hydrogels. This article gives particular importance to different novel approaches in the management of wound healing. This review draws out the data and hopes to provide understanding into traditional, current and imminent techniques and methods for wound management.

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Assessment of Frailty In Elderly Patients Attending A Multidisciplinary Wound Care Center: A Cohort Study.

10.21203/rs.3.rs-764391/v1 ◽

2021 ◽

Author(s):

Mariona Espaulella-Ferrer ◽

Joan Espaulella-Panicot ◽

Rosa Noell-Boix ◽

Marta Casals-Zorita ◽

Marta Ferrer-Sola ◽

...

Keyword(s):

Wound Healing ◽

Elderly Patients ◽

Healing Rate ◽

Wound Care ◽

Frailty Index ◽

Healing Time ◽

Venous Ulcers ◽

Healing Wounds ◽

Wound Healing Time ◽

The Relationship

Abstract Background: The incidence of frailty and chronic wounds increases with patients’ age. Knowledge of the relationship between frailty and wound healing progress is greatly lacking. Methods: The aim of this study is to characterize the degree of frailty in elderly patients attending a multidisciplinary wound care centers (MWCC). Additionally, we seek to assess the impact of frailty on the wound healing rate and wound healing time. An open cohort study was conducted on 51 consecutive patients aged > 70 years treated for wounds at an MWCC of an intermediate care hospital. The frailty score was determined according to the Frail-VIG index. Data were collected through patient questionnaires at the beginning of the study, and at six months or upon wound healing. Wounds were followed up every two weeks. To analyze the relationship between two variables was used the Chi-square test and Student’s or the ANOVA model. The t-test for paired data was used to analyze the evolution of the frailty index during follow-up.Results: A total of 51 consecutive patients were included (aged 81.1 ± 6.1 years). Frailty prevalence was 74.5% according to the Frail-VIG index (47.1% mildly frail, 19.6% moderately frail, and 7.8% severely frail). Wounds healed in 69.6% of cases at six months. The frailty index (FI) was higher in patients with non-healing wounds in comparison with patients with healing wounds (IF 0.31 ± 0.15 vs IF 0.24 ± 0.11, p=0.043). A strong correlation between FI and wound healing results was observed in patients with non-venous ulcers (FI 0.37 ± 0.13 vs FI 0.27 ± 0.10, p=0.015). However, no correlation was observed in patients with venous ulcers (FI 0.17 ± 0.09 vs FI 0.19 ± 0.09, p=0.637). Wound healing rate is statically significantly higher in non-frail patients (3,26% wound reduction/day, P25-P75 0.8-8.8%/day) in comparison with frail patients (8.9% wound reduction/day, P25-P75 3.34-18.3%/day; p=0.044). Conclusion: Frailty is prevalent in elderly patients treated at an MWCC. Frailty degree is correlated with wound healing results and wound healing time.

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Rapid epithelialisation of fetal wounds is associated with the early deposition of tenascin

Journal of Cell Science ◽

10.1242/jcs.99.3.583 ◽

1991 ◽

Vol 99 (3) ◽

pp. 583-586 ◽

Cited By ~ 5

Author(s):

D.J. Whitby ◽

M.T. Longaker ◽

M.R. Harrison ◽

N.S. Adzick ◽

M.W. Ferguson

Keyword(s):

Wound Healing ◽

Cell Migration ◽

Temporal Distribution ◽

Cell Types ◽

Skin Wound ◽

Skin Wound Healing ◽

Early Appearance ◽

Complex Process ◽

Healing Wounds ◽

Fetal Wound

Wound healing is a complex process involving the interaction of many cell types with the extracellular matrix (ECM). Fetal skin wound healing differs from that in the adult in that it occurs rapidly and without scar formation. The mechanisms underlying these differing processes may be related to the fetal environment, the stage of differentiation of the fetal cells or the ECM deposited in the wound. The spatial and temporal distribution of two components of the ECM, fibronectin and tenascin, were studied by immunostaining of cryosections from trunk wounds of fetal and adult sheep. Epithelialisation was complete earlier in the fetal wound than in the adult. The distribution of fibronectin was similar in fetal and adult wounds but tenascin was present earlier in the fetal wound. Fibronectin has several roles in wound healing including acting as a substratum for cell migration and as a mediator of cell adhesion through cell surface integrins. The attachment of fibroblasts to fibronectin is inhibited by tenascin and during development the appearance of tenascin in the ECM of migratory pathways correlates with the initiation of cell migration. Similarly, the appearance of tenascin in healing wounds may initiate cell migration. Tenascin was present in these wounds prior to cell migration and the rapid epithelialisation of fetal wounds may be due to the early appearance of tenascin in the wound.

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Simple Modelling of Extracellular Matrix Alignment in Dermal Wound Healing I. Cell Flux Induced Alignment

Journal of Theoretical Medicine ◽

10.1080/10273669808833018 ◽

1998 ◽

Vol 1 (3) ◽

pp. 175-192 ◽

Cited By ~ 14

Author(s):

Luke Olsen ◽

Philip K. Maini ◽

Jonathan A. Sherratt ◽

Ben Marchant

Keyword(s):

Extracellular Matrix ◽

Wound Healing ◽

Cell Movement ◽

Collagen Fibre ◽

Cell Types ◽

Travelling Wave ◽

Generic Model ◽

Dimensional Version ◽

Healing Wounds ◽

Dermal Wound

We present a generic model to investigate alignment due to cell movement with spefic application to collagen fibre alignment in wound healing. In particular, alignment in two orthogonal directions is considered. Numerical simulation are presented to show how alignment is affected by key parameter min the model. from a travelling wave analysis of a simplified one-dimensional version of the model we derive a first order ordinary differential equation to describe the time evolution of aligment. We conclude that in the wound healing context,faster healing wounds result in more aligment and hence more serve scarring. It is shown how the model can be extended to included orientation dependent Kinetics,multipkle cell types and several extracellular matrix materials.

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Recombinant Myxoma Virus-Derived Immune Modulator M-T7 Accelerates Cutaneous Wound Healing and Improves Tissue Remodeling

Pharmaceutics ◽

10.3390/pharmaceutics12111003 ◽

2020 ◽

Vol 12 (11) ◽

pp. 1003

Author(s):

Jordan R. Yaron ◽

Liqiang Zhang ◽

Qiuyun Guo ◽

Enkidia A. Awo ◽

Michelle Burgin ◽

...

Keyword(s):

Wound Healing ◽

Topical Treatment ◽

Immune Cell ◽

Healing Process ◽

Tissue Remodeling ◽

Myxoma Virus ◽

Cutaneous Wound Healing ◽

Cutaneous Wound ◽

Arginase 1 ◽

Healing Wounds

Complex dermal wounds represent major medical and financial burdens, especially in the context of comorbidities such as diabetes, infection and advanced age. New approaches to accelerate and improve, or “fine tune” the healing process, so as to improve the quality of cutaneous wound healing and management, are the focus of intense investigation. Here, we investigate the topical application of a recombinant immune modulating protein which inhibits the interactions of chemokines with glycosaminoglycans, reducing damaging or excess inflammation responses in a splinted full-thickness excisional wound model in mice. M-T7 is a 37 kDa-secreted, virus-derived glycoprotein that has demonstrated therapeutic efficacy in numerous animal models of inflammatory immunopathology. Topical treatment with recombinant M-T7 significantly accelerated wound healing when compared to saline treatment alone. Healed wounds exhibited properties of improved tissue remodeling, as determined by collagen maturation. M-T7 treatment accelerated the rate of peri-wound angiogenesis in the healing wounds with increased levels of TNF, VEGF and CD31. The immune cell response after M-T7 treatment was associated with a retention of CCL2 levels, and increased abundances of arginase-1-expressing M2 macrophages and CD4 T cells. Thus, topical treatment with recombinant M-T7 promotes a pro-resolution environment in healing wounds, and has potential as a novel treatment approach for cutaneous tissue repair.

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Ca-Zn-Ag Alginate Aerogels for Wound Healing Applications: Swelling Behavior in Simulated Human Body Fluids and Effect on Macrophages

Polymers ◽

10.3390/polym12112741 ◽

2020 ◽

Vol 12 (11) ◽

pp. 2741

Author(s):

Claudia Keil ◽

Christopher Hübner ◽

Constanze Richter ◽

Sandy Lier ◽

Lars Barthel ◽

...

Keyword(s):

Wound Healing ◽

Wound Care ◽

Developed Countries ◽

Swelling Behavior ◽

Wound Dressings ◽

Regulatory Function ◽

Wound Fluid ◽

Healing Wounds ◽

Injury Models

Chronic non-healing wounds represent a substantial economic burden to healthcare systems and cause a considerable reduction in quality of life for those affected. Approximately 0.5–2% of the population in developed countries are projected to experience a chronic wound in their lifetime, necessitating further developments in the area of wound care materials. The use of aerogels for wound healing applications has increased due to their high exudate absorbency and ability to incorporate therapeutic substances, amongst them trace metals, to promote wound-healing. This study evaluates the swelling behavior of Ca-Zn-Ag-loaded alginate aerogels and their metal release upon incubation in human sweat or wound fluid substitutes. All aerogels show excellent liquid uptake from any of the formulas and high liquid holding capacities. Calcium is only marginally released into the swelling solvents, thus remaining as alginate bridging component aiding the absorption and fast transfer of liquids into the aerogel network. The zinc transfer quota is similar to those observed for common wound dressings in human and animal injury models. With respect to the immune regulatory function of zinc, cell culture studies show a high availability and anti-inflammatory activity of aerogel released Zn-species in RAW 264.7 macrophages. For silver, the balance between antibacterial effectiveness versus cytotoxicity remains a significant challenge for which the alginate aerogels need to be improved in the future. An increased knowledge of the transformations that alginate aerogels undergo in the course of the fabrication as well as during wound fluid exposure is necessary when aiming to create advanced, tissue-compatible aerogel products.

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Preparation and In Vivo Study of an Anti-Infective Highly Volatile Wound Dressing Material

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.123-125.355 ◽

2010 ◽

Vol 123-125 ◽

pp. 355-358

Author(s):

Mubarak A. Khan ◽

Jahid M.M. Islam ◽

Md. Arifur Rahman ◽

H.U. Shekhar

Keyword(s):

Wound Healing ◽

Rattus Norvegicus ◽

Wound Dressing ◽

Ventral Side ◽

Gelatin Solution ◽

Spray System ◽

High Volatility ◽

Healing Wounds ◽

Antibiotic Dosing

In this research, a highly volatile membrane forming solution of gelatin with quick membrane forming property (30 sec) was prepared to investigate the suitability of solution spray system in the treatment of wound at dermal surface. A nontoxic Aromatic Ketone (AK) and a highly volatile solvent (VS) were introduced in gelatin solution to ensure high volatility. The solution was subjected to antibiotic dosing using Ciprofloxacin to ensure its anti-infectiveness. The pH of the raw gelatin solution and the highly volatile gelatin solution were 4.95 and 6.17 respectively. Viscosity of gelatin/AK/VS solution was 62.12mPa.s. Wound healing characteristic of the highly volatile solution (gelatin/AK/VS) was evaluated using a rat (Rattus norvegicus) model. Full-thickness wounds were created on the ventral side of the Rattus norvegicus and were dressed with the volatile solution and “eco-plaster” (control). Wound healing and bioadhesion were monitored in 3-days interval. The results revealed that prepared volatile solution was more effective in preventing blood loss as well as healing wounds than the conventional wound dresser.

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