scholarly journals Physicochemical and Antioxidant Properties of Gelatin and Gelatin Hydrolysates Obtained from Extrusion-Pretreated Fish (Oreochromis sp.) Scales

Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 275
Author(s):  
Wei-Cheng Shiao ◽  
Tien-Chiu Wu ◽  
Chia-Hung Kuo ◽  
Yung-Hsiang Tsai ◽  
Mei-Ling Tsai ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Extraction Yield ◽  
The Other ◽  
Antioxidant Peptides ◽  
Reverse Phase ◽  
Naturally Occurring ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Fish gelatin and its hydrolysates exhibit a variety of biological characteristics, which include antihypertensive and antioxidant properties. In this study, fish gelatins were extracted from extrusion-pretreated tilapia scales, and then subjected to analyses to determine the physicochemical properties and antioxidant activity of the extracted gelatins. Our findings indicate that TSG2 (preconditioned with 1.26% citric acid) possessed the greatest extraction yield, as well as higher antioxidant activities compared with the other extracted gelatins. Hence, TSG2 was subjected to further hydrolyzation using different proteases and ultrafiltration conditions, which yielded four gelatin hydrolysates: TSGH1, TSGH2, TSGH3, and TSGH4. The results showed that TSGH4 (Pepsin + Pancreatin and ultrafiltration < 3000 Da) had a higher yield and greater antioxidant activity in comparison with the other gelatin hydrolysates. As such, TSGH4 was subjected to further fractionation using a Superdex peptide column and two-stage reverse-phase column HPLC chromatography, yielding a subfraction TSGH4-6-2-b, which possessed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity compared with the other fractions. Further LC-ESI/MS/MS analysis of TSGH4-6-2-b suggested two novel peptides (GYDEY and EPGKSGEQGAPGEAGAP), which could have potential as naturally-occurring peptides with antioxidant properties. These promising results suggest that these antioxidant peptides could have applications in food products, nutraceuticals, and cosmetics.

scholarly journals Presumptive Relationship between Oxidoreduction Potential and Both Antibacterial and Antioxidant Activities of Herbs and Spices: Oxidoreduction Potential as a Companion Tool for Measuring the Antioxidant Activity

2018 ◽  
Vol 47 (2) ◽  
pp. 506-514 ◽  
Author(s):  
Duried ALWAZEER ◽  
Sally DHAM
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Oxidoreduction Potential ◽  
Herbs And Spices

Antibacterial and antioxidant activities form an essential component of the bioactive properties of plant products. The antibacterial and the antioxidant properties of three types of aqueous extracts of four herbs and spices were evaluated. While the total phenolic content, DPPH radical scavenging activity and oxidoreduction potential methods were used for the antioxidant properties determination, the inhibition diameters of extracts toward the growth of Staphylococcus warneri, Bacillus cereus, Enterobacter cloacae and Proteus vulgaris were evaluated. Although the phenolic content did not correlate with the antibacterial activity of plant extracts, it linearly correlated (R2 = 0.827) with DPPH scavenging activity of the studied plants following the order: sumac > ginger > rosemary > cinnamon. The oxidoreduction potential values linearly correlated with both the phenolic content (R2 ≈ 0.88) and DPPH scavenging activity values (R2 ≈ 0.96). The oxidoreduction potential could be proposed as a useful companion tool combined with other techniques when determining the antioxidant activity of plant extracts and food products is considered.

scholarly journals ANTIOXIDANT PROFILE AND PHYTOCHEMICAL CONTENT OF DIFFERENT PARTS OF SUPER RED DRAGON FRUIT (HYLOCEREUS COSTARICENSIS) COLLECTED FROM WEST JAVA-INDONESIA

2017 ◽  
Vol 10 (12) ◽  
pp. 290 ◽  
Author(s):  
Irda Fidrianny ◽  
Nadia Ilham ◽  
Rika Hartati
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Phytochemical Content ◽  
Dragon Fruit ◽  
Dpph Method ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Different Parts

Objectives: The goals of this research were to observe antioxidant properties from different parts of super red dragon fruit (Hylocereus costaricensis) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS).Methods: Antioxidant activities were determined using DPPH and ABTS assays, total phenolic content (TPC) using Folin–Ciocalteu reagent, flavonoid content by Chang’s method.Results: Inhibitory concentration 50% (IC50) of DPPH scavenging activity of all of the extracts in the range of 2.69 μg/ml was −94.17 μg/ml. The ethyl acetate peel extract of super red dragon fruit expressed the highest TPC (4.56 g GAE/100 g) and the highest total flavonoid content (12.63 g QE/100 g). TPC in flesh extract of super red dragon fruit had a negative and significant correlation with their IC50 of ABTS. The IC50 of DPPH and IC50 of ABTS of flesh extract of super red dragon fruit showed positive and significant correlation.Conclusion: All different parts extracts of super red dragon fruit (except n-hexane flesh extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in flesh extract of super red dragon fruit were the major contributor in antioxidant activities by ABTS method. DPPH and ABTS showed linear results in antioxidant activities of super red dragon fruit flesh extract.

scholarly journals Antioxidant Activity and Anti-Nutritional Factors in Acacia Nilotica Gum

2021 ◽  
Vol 11 (6) ◽  
pp. 42-51
Author(s):  
Kazi Layla Khaled ◽  
Rukshana Irani
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Nutritional Composition ◽  
Acacia Nilotica ◽  
Nutritional Factors ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Relationship Of ◽  
Gallic Acid Equivalent ◽  
Food Commodity

The rapidly growing civilization and economy need the judicious usage of floral and faunal heritage. Acacia nilotica (Family: Fabaceae) is a beneficial plant species and each part of it exhibits numerous biological and pharmacological effects. The gum exudates of Acacia nilotica trees are underutilized plant products that can be explored for their potential as a therapeutic and curative agent. Its nutritional composition, interaction with anti-nutritional factors and antioxidants can determine its utility as a food. The present investigation was aimed to estimate the anti-nutritional factors and antioxidant activity of Acacia nilotica gum. It also focused on establishing a relationship of the obtained data with the ethnomedicinal and pharmacological properties claimed in the literature. The analysis was carried out using standard methods and protocols involving spectrophotometric and gravimetric principles. The anti-nutritional components like tannin, trypsin inhibitor, phytate, oxalate, total saponin, steroidal saponins, alkaloid content of Acacia nilotica gum was found to be 0.2369±0.0354 g tannic acid equivalent/100 g, 0.0108±0.0037 TIU/mg, 156.3333±9.4516 mg/100 g, 132.5±28.3152mg/100 g, 12.7183±6.7788 mg diosgenin equivalent /g, 0.0705±0.01 mg diosgenin equivalent /g, 0.1333±0.0577 g/100 g respectively. The amounts of total phenols and flavonoids was determined to be 8.0033±2.7211 mg of gallic acid equivalent/g sample, 0.0458±0.0186 mg quercetin equivalent /g sample respectively with a significant DPPH Scavenging activity. The results indicate that the quantity of anti-nutritional factors in gum is within the range of the contents of anti-nutritional factors found in commonly consumed foods. The substantial presence of phenol, flavonoids and DPPH activity in the gum indicates the presence of high antioxidant properties. The presence of anti-nutritional factors and antioxidants in favourable levels can impart diverse physiological responses. The present study concluded that Acacia nilotica gum can be used as a nutritious food commodity with a plethora of recuperative activities.

scholarly journals Antioxidant Activity and Anti-Nutritional Factors in Acacia Nilotica Gum

2021 ◽  
Vol 11 (6) ◽  
pp. 42-51
Author(s):  
Layla Khaled Kazi ◽  
Rukshana Irani
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Nutritional Composition ◽  
Acacia Nilotica ◽  
Nutritional Factors ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Relationship Of ◽  
Gallic Acid Equivalent ◽  
Food Commodity

The rapidly growing civilization and economy need the judicious usage of floral and faunal heritage. Acacia nilotica (Family: Fabaceae) is a beneficial plant species and each part of it exhibits numerous biological and pharmacological effects. The gum exudates of Acacia nilotica trees are underutilized plant products that can be explored for their potential as a therapeutic and curative agent. Its nutritional composition, interaction with anti-nutritional factors and antioxidants can determine its utility as a food. The present investigation was aimed to estimate the anti-nutritional factors and antioxidant activity of Acacia nilotica gum. It also focused on establishing a relationship of the obtained data with the ethnomedicinal and pharmacological properties claimed in the literature. The analysis was carried out using standard methods and protocols involving spectrophotometric and gravimetric principles. The anti-nutritional components like tannin, trypsin inhibitor, phytate, oxalate, total saponin, steroidal saponins, alkaloid content of Acacia nilotica gum was found to be 0.2369±0.0354 g tannic acid equivalent/100 g, 0.0108±0.0037 TIU/mg, 156.3333±9.4516 mg/100 g, 132.5±28.3152mg/100 g, 12.7183±6.7788 mg diosgenin equivalent /g, 0.0705±0.01 mg diosgenin equivalent /g, 0.1333±0.0577 g/100 g respectively. The amounts of total phenols and flavonoids was determined to be 8.0033±2.7211 mg of gallic acid equivalent/g sample, 0.0458±0.0186 mg quercetin equivalent /g sample respectively with a significant DPPH Scavenging activity. The results indicate that the quantity of anti-nutritional factors in gum is within the range of the contents of anti-nutritional factors found in commonly consumed foods. The substantial presence of phenol, flavonoids and DPPH activity in the gum indicates the presence of high antioxidant properties. The presence of anti-nutritional factors and antioxidants in favourable levels can impart diverse physiological responses. The present study concluded that Acacia nilotica gum can be used as a nutritious food commodity with a plethora of recuperative activities.

scholarly journals Antioxidant Properties of Crude Extract, Partition Extract, and Fermented Medium of Dendrobium sabin Flower

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Farahziela Abu ◽  
Che Norma Mat Taib ◽  
Mohamad Aris Mohd Moklas ◽  
Sobri Mohd Akhir
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Correlation Relationship ◽  
Good Potential ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Antioxidant properties of crude extract, partition extract, and fermented medium from Dendrobium sabin (DS) flower were investigated. The oven-dried DS flower was extracted using 100% methanol (w/v), 100% ethanol (w/v), and 100% water (w/v). The 100% methanolic crude extract showed the highest total phenolic content (40.33 ± mg GAE/g extract) and the best antioxidant properties as shown by DPPH, ABTS, and FRAP assays. A correlation relationship between antioxidant activity and total phenolic content showed that phenolic compounds were the dominant antioxidant components in this flower extract. The microbial fermentation on DS flower medium showed a potential in increasing the phenolic content and DPPH scavenging activity. The TPC of final fermented medium showed approximately 18% increment, while the DPPH of fermented medium increased significantly to approximately 80% at the end of the fermentation. Dendrobium sabin (DS) flower showed very good potential properties of antioxidant in crude extract and partition extract as well as better antioxidant activity in the flower fermented medium.

scholarly journals Antioxidant activity of Dianthus chinensis flowers processed by ionizing radiation

2019 ◽  
Vol 7 (2A) ◽  
Author(s):  
Amanda Cristina Ramos Koike
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolics ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Bioactive Components ◽  
Irradiation Treatment ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Β Carotene

The edible flowers are increasingly used in culinary preparations, which require new approaches to improve their conservation and safety. Irradiation treatment is safe and an effective alternative for food conservation. Indeed, it can also guarantee food quality, increasing shelf-life and disinfestation of it. This technology gives us a versatile way to get good quality food, reducing post-harvest losses. Dianthus chinensis flowers, popularly known as Chinese pink, are widely used in culinary preparations, being also acknowledged for their bioactive components and antioxidant properties. The purpose of this study was to evaluate the antioxidant activity of D. chinensis flowers submitted to electron beam and gamma irradiation at 0, 0.5, 0.8 and 1 kGy. The antioxidant properties were evaluated through 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, reducing power and β-carotene bleaching inhibition assays. Total phenolics were also determined by the Folin-Ciocalteu assay. The antioxidant activity was higher for irradiated samples, especially those treated with 0.8 and 1 kGy, independently of the radiation source, which showed the highest capacity to inhibit β-carotene bleaching. Accordingly, the applied irradiation treatments seemed to represent feasible technology to preserve the quality of edible flower petals, being able to improve the antioxidant activity

scholarly journals Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
THEERAPHAN CHUMROENPHAT ◽  
Surapon Saensouk ◽  
PIYAPORN SAENSOUK
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Plant Parts ◽  
Ferric Reducing Antioxidant Power ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Abstract. Chumroenphat T, Saensouk S, Saensouk P. 2021. Chemical composition and antioxidant activity of three species of Cornukaempferia in Thailand. Biodiversitas 22: 4036-4044. Genus Cornukaempferia (Zingiberaceae) were used as medicinal plants in Thailand. Cornukaempferia has never been reported the chemical compounds and antioxidant activity before. This study was designed to identify the chemical composition and antioxidant activities in root, rhizome, stem, and leaves of Cornukaempferia aurantiflora, C. larsenii, and C. longipetiolata. DPPH scavenging activity and Ferric reducing antioxidant power (FRAP) assays assessed the chemical composition and antioxidant activity. The results showed that total phenolic content (TPC) and total flavonoid content (TFC) found different plant parts obtained in this study were in the order of root>rhizomes>leaves>?stems. The C. aurantiflora had a high TPC of 416.39 mg GAE/g dry weight, while individual phenolics, such as vanillic acid at 256.90 mg/g dry weight, showed high contents in the leaf part. For C. larsenii,  TFC and individual flavonoids, such as catechin, have high 62.52 and 56.47 mg/100g dry weight values in the roots, respectively. The antioxidant activities in different species of different plant parts (DPPH: root>leaves?> rhizomes?>?stems; FRAP: leaves?>root>?rhizomes?>?stems). The genus Cornukaempferia is a rich source of bioactive compounds and antioxidant activity. Therefore, the genus Cornukaempferia will be used as medicine and food to produce products with health benefits in the future.

Chemical Composition and Antiproliferative and Antioxidant Activities of Methanolic Extract of Alcea setosa A. Malvaceae

2021 ◽  
pp. 6447-6454
Author(s):  
Hana R. Bajes ◽  
Sawsan A. Oran ◽  
Yasser K. Bustanji
Keyword(s):  
Antioxidant Activity ◽  
Cancer Cells ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Human Fibroblasts ◽  
Wild Plant ◽  
Scavenging Activity ◽  
Medicinal Uses ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Alcea setosa A. (Malvaceae) is a wild plant that grows in Jordan and have several traditional medicinal uses. This study aims to collect and chemically analyze the methanolic extract from Alcea setosa A. from Jordan and to evaluate its cytotoxic and antioxidant activity against human breast cancer cells (T47D), colorectal adenocarcinoma cells (CACO2), and normal human fibroblasts (MRC5). The extract was extracted by methanol solvent and analyzed by liquid chromatography coupled with a mass spectrometer. Cell viability was assessed using trypan blue, neutral red, and MTT assays, and antioxidant activity was evaluated using DPPH scavenging activity assay. A total of 290 compounds, 12 among which were identified when compared to available standards, the extract contained six flavons derivatives, Two fatty acids, one ketone derivative, one flavonol derivative, one organic acid, and one coumarin derivative. The results also revealed that the IC50 values of the viability assays were higher among normal cells compared to the human cancer cell lines, and the viability inhibition was significant at higher concentrations compared to untreated cells. Nevertheless, moderate antioxidant activity was observed for the extract in the DPPH scavenging activity test. To sum up, this study indicates that samples of A. setosa collected from Jordan is likely to be an effective antioxidant, is optimistically potential to be utilized in breast and colon cancers treatment due to its preferential cytotoxicity against cancer cells.

scholarly journals Peptides of marine animals as a potential source of natural antioxidants

2017 ◽  
Vol 189 (2) ◽  
pp. 192-203
Author(s):  
Ekaterina P. Karaulova ◽  
Anna I. Chepkasova
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Protein Hydrolysate ◽  
Antioxidant Properties ◽  
Low Molecular Weight ◽  
Scavenging Activity ◽  
Marine Animals ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Peptides extracted from tissues of 11 shellfish species ( Corbicula japonica, Mercenaria stimpsoni, Anadara broughtoni, Patinopecten yessoesis, Crenomytilus grayanus, Spisula sachalinensis, Maktra chinensis, Serripes groenlandicus, Glycymeris yessoensis, Callista brevisiphonata, Dosinia japonica ) are tested for their antioxidant properties in different in vitro conditions. Frozen muscles and viscera of the shellfish were minced and subjected to water extraction of proteins and to hydrolysis with Protamex 1.5 MG. The peptides antioxidant activity was evaluated by assessing of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity on free radicals generated in oxidative systems. In the experiment, the high perfomance liquid chromatography (HPLC) system (Agilent Technologies 1260) included the frame TSKgel G 3000PWXL, the flow rate was 0.1 mL/min (0.1 N NaCI-20 mM Tris-HCI, pH 8.0), the detection was made under 280 nm. Molecular weight of the proteins was determined by comparison of their retention time with pure protein standards. The enzyme activity was high in conditions of pH in the range of 5.5-8.0, with the maximum under pH 7.0. Among 11 shellfish species, the highest antioxidant activity in water extract was observed for C. japonica , M. stimpsoni , C. brevisiphonata , G. yessoensis , and C. grayanus : 86.3, 68.7, 72.3, 90.2, and 67.5 mg of ascorbic acid/g, respectively. The DPPH radical scavenging activity increased with increasing of the hydrolysis degree. The 1-step hydrolysis with Protamex enhanced the DPPH scavenging activity for all samples, with the highest value for the protein hydrolysate of M. stimpsoni tissues (215 mg of ascorbic acid/g). Size of generated peptides is important for the antioxidant activity therefore molecular weight distribution of the peptides during the proteolysis was investigated using HPLC. Portion of the low molecular weight peptides (≤ 1 kDa) in the protein hydrolysate was increased in 4-14 % as compared with the starting protein. The antioxidant activity correlated positively with the number of low molecular weight peptides in protein hydrolysates.

scholarly journals Phytochemical screening and antioxidant activities of Methanol extracts from eight Syzygium species

2020 ◽  
Vol 16 (1) ◽  
pp. 13-17
Author(s):  
Siti Awanis Abdullah ◽  
Shajarahtunnur Jamil
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Antioxidant Activities ◽  
Ferric Ion ◽  
Phytochemical Screening ◽  
Abts Assay ◽  
Methanol Extracts ◽  
Crude Extracts ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

This study was carried out to investigate the phytochemical screening of the methanol crude extracts of the leaves, twigs, and heartwoods of eight Syzygium species (S. filiforme var. filiforme, S. papillosum, S. griffithii, S. pseudoformosum, S. claviflorum var. claviflorum, S. glaucum, S. syzygioides, and Eugenia Sp. 57). The analysis revealed the presence of alkaloids, flavonoids, phenols, steroids, terpenes, and tannins except saponins in all plant extracts. Evaluation of the antioxidant activities were tested using DPPH, FRAP, and ABTS assays. All plant extracts showed good DPPH scavenging activity with the percentage inhibition more than 70 % at 100 ppm. The leaves and twigs of the methanol extracts of S. griffithii exhibited the most potent ferric ion reducer ranging from 1.03 ± 0.03 mM to 7.52 ± 0.49 mM and 1.43 ± 0.12 mM to 9.68 ± 0.38 mM FRAP equivalent, respectively. The heartwoods crude extract of S. syzygioides showed the most potent ferric ion reducer ranging from 1.20 ± 0.01 mM to 10.47 ± 0.12 mM FRAP equivalent. All of the leaves crude extracts showed potential antioxidant activities towards ABTS assay. The methanol extracts of the leaves of S. glaucum showed good SC50 value (116.90 mM) followed by S. papillosum (S2LM, SC50 value = 132.70 mM) and Eugenia Sp. 57 (S4LM, SC50 value = 143.10 mM). The twigs crude extracts of S. syzygioides (S8TM) and S. griffithii (S3TM) showed excellent antioxidant activity towards ABTS assay with the SC50 values of 130.60 mM and 145.40 mM, respectively. The crude extracts of S. syzygioides exhibited good antioxidant activity among the heartwoods extracts with the SC50 value of 96.20 mM.

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