scholarly journals Study of the Fabrication Technology of Hybrid Microfluidic Biochips for Label-Free Detection of Proteins

Micromachines ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 20
Author(s):  
Nikita Sitkov ◽  
Tatiana Zimina ◽  
Alexey Kolobov ◽  
Evgeny Sevostyanov ◽  
Valentina Trushlyakova ◽  
...  
Keyword(s):  
Technological Process ◽  
Biological Fluid ◽  
Protein Structures ◽  
Microfluidic System ◽  
Diagnostic Tools ◽  
Fluorometric Detection ◽  
Label Free ◽  
Protein Markers ◽  
Label Free Detection ◽  
Microfluidic Biochips

A study of the peculiarities and a comparative analysis of the technologies used for the fabrication of elements of novel hybrid microfluidic biochips for express biomedical analysis have been carried out. The biochips were designed with an incorporated microfluidic system, which enabled an accumulation of the target compounds in a biological fluid to be achieved, thus increasing the biochip system’s sensitivity and even implementing a label-free design of the detection unit. The multilevel process of manufacturing a microfluidic system of a given topology for label-free fluorometric detection of protein structures is presented. The technological process included the chemical modification of the working surface of glass substrates by silanization using (3-aminopropyl) trimethoxysilane (APTMS), formation of the microchannels, for which SU-8 technologies and a last generation dry film photoresist were studied and compared. The solid-state phosphor layers were deposited using three methods: drop application; airbrushing; and mechanical spraying onto the adhesive surface. The processes of sealing the system, installing input ports, and packaging using micro-assembly technologies are described. The technological process has been optimized and the biochip was implemented and tested. The presented system can be used to design novel high-performance diagnostic tools that implement the function of express detection of protein markers of diseases and create low-power multimodal, highly intelligent portable analytical decision-making systems in medicine.

scholarly journals Detection of Myoglobin with an Open-Cavity-Based Label-Free Photonic Crystal Biosensor

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Bailin Zhang ◽  
Juan Manuel Tamez-Vela ◽  
Steven Solis ◽  
Gilbert Bustamante ◽  
Ralph Peterson ◽  
...  
Keyword(s):  
Photonic Crystal ◽  
Point Of Care ◽  
Microfluidic System ◽  
Cardiac Biomarkers ◽  
Detection Sensitivity ◽  
Label Free ◽  
Binding Assays ◽  
Biochemical Modification ◽  
Label Free Detection ◽  
Biomolecular Assays

The label-free detection of one of the cardiac biomarkers, myoglobin, using a photonic-crystal-based biosensor in a total-internal-reflection configuration (PC-TIR) is presented in this paper. The PC-TIR sensor possesses a unique open optical microcavity that allows for several key advantages in biomolecular assays. In contrast to a conventional closed microcavity, the open configuration allows easy functionalization of the sensing surface for rapid biomolecular binding assays. Moreover, the properties of PC structures make it easy to be designed and engineered for operating at any optical wavelength. Through fine design of the photonic crystal structure, biochemical modification of the sensor surface, and integration with a microfluidic system, we have demonstrated that the detection sensitivity of the sensor for myoglobin has reached the clinically significant concentration range, enabling potential usage of this biosensor for diagnosis of acute myocardial infarction. The real-time response of the sensor to the myoglobin binding may potentially provide point-of-care monitoring of patients and treatment effects.

scholarly journals Toward Development of a Label-Free Detection Technique for Microfluidic Fluorometric Peptide-Based Biosensor Systems

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 691
Author(s):  
Nikita Sitkov ◽  
Tatiana Zimina ◽  
Alexander Kolobov ◽  
Vladimir Karasev ◽  
Alexander Romanov ◽  
...  
Keyword(s):  
Troponin I ◽  
Troponin T ◽  
Detection System ◽  
Label Free ◽  
Protein Markers ◽  
Peptide Aptamers ◽  
Registration System ◽  
Biosensor System ◽  
Label Free Detection ◽  
Selection Of

The problems of chronic or noncommunicable diseases (NCD) that now kill around 40 million people each year require multiparametric combinatorial diagnostics for the selection of effective treatment tactics. This could be implemented using the biosensor principle based on peptide aptamers for spatial recognition of corresponding protein markers of diseases in biological fluids. In this paper, a low-cost label-free principle of biomarker detection using a biosensor system based on fluorometric registration of the target proteins bound to peptide aptamers was investigated. The main detection principle considered includes the re-emission of the natural fluorescence of selectively bound protein markers into a longer-wavelength radiation easily detectable by common charge-coupled devices (CCD) using a specific luminophore. Implementation of this type of detection system demands the reduction of all types of stray light and background fluorescence of construction materials and aptamers. The latter was achieved by careful selection of materials and design of peptide aptamers with substituted aromatic amino acid residues and considering troponin T, troponin I, and bovine serum albumin as an example. The peptide aptamers for troponin T were designed in silico using the «Protein 3D» (SPB ETU, St. Petersburg, Russia) software. The luminophore was selected from the line of ZnS-based solid-state compounds. The test microfluidic system was arranged as a flow through a massive of four working chambers for immobilization of peptide aptamers, coupled with the optical detection system, based on thick film technology. The planar optical setup of the biosensor registration system was arranged as an excitation-emission cascade including 280 nm ultraviolet (UV) light-emitting diode (LED), polypropylene (PP) UV transparent film, proteins layer, glass filter, luminophore layer, and CCD sensor. A laboratory sample has been created.

scholarly journals Integrált optikai szenzor biológiai minták gyors analíziséhez

2015 ◽  
Vol 156 (52) ◽  
pp. 2116-2119
Author(s):  
Anna Mathesz ◽  
Sándor Valkai ◽  
Orsolya Sipos ◽  
Balázs Stercz ◽  
Béla Kocsis ◽  
...  
Keyword(s):  
Medical Diagnostics ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Label Free ◽  
Microbial Forensics ◽  
Wide Range ◽  
Label Free Detection ◽  
Integrated Optical ◽  
Measuring Techniques ◽  
Escherichia Coli Bacteria

Introduction: In the medical diagnostics of bacteria, the rapid detection of pathogenic microorganisms from body fluids is one of the most important tasks. The majority of the modern measuring techniques are based on specific labels bound to the bacteria. However, this strategy usually assumes a rather time-consuming procedure involving several steps (e.g., the widely used enzyme-linked immunosorbent assay normally consists of 5 consecutive steps). Hence, there is an urgent need for the elaboration of rapid, “label-free” techniques, that are often based on Lab-on-a-chip devices. Aim: In this paper, the authors report on the development of a biosensor based on a miniature, integrated optical Mach–Zehnder interferometer. Method: Functionalization of the measuring arm of the sensor by antibodies, made the rapid and specific label-free detection of pathogens feasible. Results: Using the combination of the interferometer with a microfluidic system, the device was able to detect Escherichia coli bacteria at concentrations as low as 106 colony forming unit/ml within minutes. Conclusions: This makes the newly developed biosensor a promising device for a wide range of applications, not only in medical microbiology, but microbial forensics, criminal investigations, bio-terrorism threats and in environmental studies as well. Orv. Hetil., 2015, 156(52), 2116–2119.

A facile water-stable MOF-based “off–on” fluorescent switch for label-free detection of dopamine in biological fluid

2017 ◽  
Vol 5 (13) ◽  
pp. 2524-2535 ◽  
Author(s):  
Yue Cheng ◽  
Jie Wu ◽  
Chao Guo ◽  
Xin-Ge Li ◽  
Bin Ding ◽  
...  
Keyword(s):  
Surface Modification ◽  
Biological Fluid ◽  
Label Free ◽  
Fluorescent Switch ◽  
Label Free Detection

An Abtz–CdI2–MOF was developed as an “off–on” fluorescent switch for label-free detection of dopamine without any surface modification or functionalization.

A NANOSCALE POROUS SILICON MICROCAVITY BIOSENSOR FOR NOVEL LABEL-FREE TUBERCULOSIS ANTIGEN–ANTIBODY DETECTION

NANO ◽  
2012 ◽  
Vol 07 (06) ◽  
pp. 1250049 ◽  
Author(s):  
BAO WU ◽  
GUOGUANG RONG ◽  
JUNWEI ZHAO ◽  
SHULIN ZHANG ◽  
YONGXIN ZHU ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Diagnostic Technique ◽  
Detection Methods ◽  
Antibody Detection ◽  
Diagnostic Tools ◽  
World Population ◽  
Label Free ◽  
Mycobacterium Tuberculosis Infection ◽  
Sensing Platform ◽  
Label Free Detection

One third of the world population is estimated to have Mycobacterium tuberculosis infection. It is urgent to develop a rapid, inexpensive and convenient diagnostic method for detection of tuberculosis. Porous silicon material has taken more and more attention in recent years for biosensing applications and some useful results have been obtained. In this paper, we report the feasibility of applying porous silicon microcavity biosensor in a novel and relatively rapid serodiagnostic approach. Nowadays, most of serodiagnostic tests are based on labeled detection. Applying label-free detection methods can help develop fast and efficient tuberculosis diagnostic tools, which can meet the current demand. In this study, we use this label-free sensing platform (i.e., porous silicon microcavity) to detect the interaction between 16 kDa antigen and anti-16 kDa antibody. Through a series of experiments, we verify the specificity and examine the sensitivity of this new diagnostic technique. The results show that it is feasible to apply porous silicon microcavity in the tests of tuberculosis.

scholarly journals Integrated optical biosensor for rapid detection of bacteria

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Anna Mathesz ◽  
Sándor Valkai ◽  
Attila Újvárosy ◽  
Badri Aekbote ◽  
Orsolya Sipos ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Optical Biosensor ◽  
Medical Diagnostics ◽  
Microfluidic System ◽  
Body Fluids ◽  
Label Free ◽  
Label Free Detection ◽  
Integrated Optical ◽  
Escherichia Coli Bacteria

AbstractIn medical diagnostics, rapid detection of pathogenic bacteria from body fluids is one of the basic issues. Most state-of-the-art methods require optical labeling, increasing the complexity, duration and cost of the analysis. Therefore, there is a strong need for developing selective sensory devices based on label-free techniques, in order to increase the speed, and reduce the cost of detection. In a recent paper, we have shown that an integrated optical Mach-Zehnder interferometer, a highly sensitive all-optical device made of a cheap photopolymer, can be used as a powerful lab-on-a-chip tool for specific, labelfree detection of proteins. By proper modifications of this technique, our interferometric biosensor was combined with a microfluidic system allowing the rapid and specific detection of bacteria from solutions, having the surface of the sensor functionalized by bacterium-specific antibodies. The experiments proved that the biosensor was able to detect Escherichia coli bacteria at concentrations of 106 cfu/ml within a few minutes, that makes our device an appropriate tool for fast, label-free detection of bacteria from body fluids such as urine or sputum. On the other hand, possible applications of the device may not be restricted to medical microbiology, since bacterial identification is an important task in microbial forensics, criminal investigations, bio-terrorism threats and in environmental studies, as well.

Simulation of Whispering-Gallery Mode Microsensing in a Microfluidic System

2009 ◽  
Author(s):  
Lei Huang ◽  
Zhixiong Guo
Keyword(s):  
Electromagnetic Waves ◽  
Whispering Gallery Mode ◽  
Resonance Wavelength ◽  
Microfluidic System ◽  
Wavelength Shift ◽  
Label Free ◽  
Helmholtz Equations ◽  
Fluidic Channel ◽  
Whispering Gallery ◽  
Label Free Detection

Label-free detection using a whispering-gallery mode biosensor in a micro fluidic channel is simulated. The analyte transport in the solution is controlled by applied electric potentials and diffusion. The finite element method is employed for solving the charged species transportation equations, the Poisson equation, the equations of conservation of momentum and energy, and the Helmholtz equations for electromagnetic waves. The adsorption process of analyte on the micro resonator surface is monitored by the resonance wavelength shift in the sensor. Shift caused by temperature variation due to Joule heating is found to be negligible compared to that induced by analyte deposition. The deposition induced shifts behave in a manner similar to Langmuir-like adsorption kinetics. A linear correlation between the frequency shift and the analyte concentration in the solution is obtained. The applied voltage is found to affect the adsorption capability; and thus, the sensor sensitivity. Detection of very low concentration to the sub-ppm level using the sensor is demonstrated.

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

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