Dietary Saccharomyces cerevisiae boulardii CNCM I-1079 Positively Affects Performance and Intestinal Ecosystem in Broilers during a Campylobacter jejuni Infection

In poultry production, probiotics have shown promise to limit campylobacteriosis at the farm level, the most commonly reported zoonosis in Europe. The aim of this trial was to evaluate the effects of Saccharomyces supplementation in Campylobacter jejuni challenged chickens on performance and intestinal ecosystem. A total of 156 day old male Ross 308 chicks were assigned to a basal control diet (C) or to a Saccharomyces cerevisiae boulardii CNCM I-1079 supplemented diet (S). All the birds were orally challenged with C. jejuni on day (d) 21. Live weight and growth performance were evaluated on days 1, 21, 28 and 40. The histology of intestinal mucosa was analyzed and the gut microbiota composition was assessed by 16S rRNA. Performance throughout the trial as well as villi length and crypt depth were positively influenced by yeast supplementation. A higher abundance of operational taxonomic units (OTUs) annotated as Lactobacillus reuteri and Faecalibacterium prausnitzii and a lower abundance of Campylobacter in fecal samples from S compared to the C group were reported. Supplementation with Saccharomyces cerevisiae boulardii can effectively modulate the intestinal ecosystem, leading to a higher abundance of beneficial microorganisms and modifying the intestinal mucosa architecture, with a subsequent improvement of the broilers’ growth performance.

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Effect of Yeast Type and Dose on Growth Performance and Nutrient Digestibility of Growing Rabbits Fed Maize or Barley

Indian Journal of Animal Research ◽

10.18805/ijar.b-1331 ◽

2021 ◽

Author(s):

Amr M.A. Elmasry ◽

Luis A. Miranda ◽

Germán Mendoza ◽

Fernando X. Plata

Keyword(s):

Saccharomyces Cerevisiae ◽

Body Weight ◽

Growth Performance ◽

Control Diet ◽

Average Daily Gain ◽

Nutrient Digestibility ◽

Feed Conversion ◽

Final Body Weight ◽

Positive Effects ◽

Yeast Concentration

Background: The effects of the probiotics vary with the doses and strains used for this reason, a study was conducted over 35 days to determine the effect of live yeast supplementation in the diet of growing rabbits on growth performance and apparent nutrient digestibility.Methods: A total of 60 New Zealand and California rabbits (5 weeks old, average body weight 0.881±0.015 kg) were housed in 30 cages. They were fed with a control diet based on alfalfa and maize or barley grains and supplemented with Procreatin®7 (Saccharomyces cerevisiae CNCM I 1077) or Biosaf® SC47 (Saccharomyces cerevisiae NCYC Sc47) at three doses, namely 0, 6.4, or 12.8 ×109 CFU per kg basal diet.Result: The higher yeast concentration had a positive effect (P less than 0.01) on final body weight (BW), average daily gain (ADG) and carcass weight (CW). However, there were no significant differences in dry matter intake. As a result of this, the feed conversion ratio (FCR) decreased (P less than 0.01). Significant increases (P less than 0.05) in nutrient digestibility (DM, OM, NDF and ADF) were observed with both grains. The use of yeast in maize and barley-based diets of rabbits has positive effects on growth performance and nutrient digestibility.

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Intestinal morphology and growth performance of the Indonesian indigenous crossbred chickens supplemented with formic acid and Saccharomyces cerevisiae

Journal of the Indonesian Tropical Animal Agriculture ◽

10.14710/jitaa.45.4.348-355 ◽

2020 ◽

Vol 45 (4) ◽

pp. 348-355

Author(s):

S. N. Erya ◽

H. I. Wahyuni ◽

T. Yudiarti ◽

E. Widiastuti ◽

S. Sugiharto

Keyword(s):

Saccharomyces Cerevisiae ◽

Weight Gain ◽

Formic Acid ◽

Growth Performance ◽

Crude Protein ◽

Control Diet ◽

Protein Digestibility ◽

Nutrient Digestibility ◽

Intestinal Morphology ◽

Intestinal Ecology

The study investigated gut ecology and morphology of the Indonesian indigenous crossbred chickens (IICC) supplemented with the combination of formic acid and Saccharomyces cerevisiae. Two hundreds day-old IICC were distributed to T0 (control diet), T1 (T0 + 0.2% formic acid), T2 (T0 + 0.3% S. cerevisiae), T3 (T0 + 0.2% formic acid and 0.3% S. cerevisiae). Excreta was collected at week 8, while intestinal ecology and morphology were determined at week 9. In duodenum, T3 chicks showed higher and wider (P<0.05) villi. The T2 and T3 chicks showed deeper (P<0.05) crypt than that of T0. The jejunal villi was higher (P<0.05) in T3 than in T0. The T3 chicks had deeper (P<0.05) crypt compared to other groups. In ileum, the villi height was lowest (P<0.05) in T0. The crypt was deeper (P<0.05) in T3 than in other. Crude protein digestibility coefficient was highest (P<0.05), while fecal protein was loswest (P<0.05) in T1 than in other groups. Compared to T0, the treated IICC showed higher (P<0.05) weight gain and feed intake with T3 had the highest gain and intake but gain:feed rasio was the lowest (P>0.05). IIn conclusion, the inclusion of formic acid and S. cerevisiae in diets improved intestinal ecology and morphology. The IICC chickens fed with formic acid and S. cerevisiae exhibited improved growth performance and nutrient digestibility.

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Choline and Bile Acids Mixture Improved Growth Performance and Intestinal Immunity via Altering Gut Microbiota and Bacterial Metabolites in the Colon of Weaned Piglets.

10.21203/rs.3.rs-32927/v1 ◽

2020 ◽

Author(s):

Yueqin Qiu ◽

Kebiao Li ◽

Shilong Liu ◽

Li Wang ◽

Kaiguo Gao ◽

...

Keyword(s):

Gut Microbiota ◽

Growth Performance ◽

Bile Acids ◽

Control Diet ◽

Choline Chloride ◽

Microbiota Composition ◽

Weaned Piglets ◽

Bacterial Metabolites ◽

G Ratio ◽

Gut Microbiota Composition

Abstract Background: Choline or bile acids has many beneficial roles in physiological function. However, little was known about growth performance, intestinal mucosal function and microbiota-host interactions of weaned piglets in response to choline or bile acids supplementation. This study aimed to investigate the effect of choline and bile acids mixtures (ChB) supplementation on growth performance, intestinal mucosal barrier function, gut microbiota and bacterial metabolites of weaned piglets. One hundred and twenty-eight crossbred (Duroc × Landrace × Large White) weaned piglets (initial body weight: approximately 8 kg; 21 d of age) were randomly allocated to four different dietary treatments(a control diet (Control) and the other three groups were control diet supplemented with 800 mg/kg choline chloride (choline), 500 mg/kg bile acids (bile acids) or 800 mg/kg choline chloride plus 500 mg/kg bile acids (ChB), respectively) and for 28-d feeding trail. Results: ChB significantly increased average daily gain (ADG) and reduced feed/gain (F/G) ratio, associated with elevation of lipase activity and total bile acids level in ileal digesta compared with control diet. Additionally, ChB altered colonic microbiota by increasing the relative abundance of Lactobacillus and Faecalibacterium , and decreasing the relative abundances of unidentified-Clostridiales , Parabacteroides and Unidentified-Ruminococcaceae , when compared with control diet. Meanwhile, ChB increased the butyrate level and decreased the production of bile acid profiles in the colonic digesta. Besides, feeding ChB improved gut immunity, as reflected by increasing the abundance of IL-10 , FXR and mucin2 transcript, while downregulated expression of TLR4 , MyD88 , NF-κBp65 and TNF-α genes in the intestinal mucosa. Quantitative proteomics of jejunal mucosa further showed that ChB regulated the proteins that were related to inflammatory response. Furthermore, the changes in the ADG and genes expression were associated with alteration of gut microbiota composition and their metabolites. Conclusions: Collectively, our findings demonstrated that choline and bile acids mixture may improve the growth performance and intestinal immune response of weaned piglets through alteration of gut microbiota composition and bacterial metabolites, which promoted gut health.

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Activities of enzymes of the pancreas, and the lumen and mucosa of the small intestine in growing broiler cockerels fed on tannin-containing diets

British Journal Of Nutrition ◽

10.1079/bjn19910080 ◽

1991 ◽

Vol 65 (2) ◽

pp. 189-197 ◽

Cited By ~ 70

Author(s):

A. E. Ahmed ◽

R. Smithard ◽

M. Ellis

Keyword(s):

Small Intestine ◽

Intestinal Mucosa ◽

Amylase Activity ◽

Control Diet ◽

Live Weight ◽

Intestinal Lumen ◽

Trypsin Activity ◽

Pancreas Weight ◽

Vegetable Tannins

Diets containing vegetable tannins, predominantly hydrolysable gallotannins, at levels of 13.5, 25 and 50 g/kg were fed to growing broiler cockerels to examine their effect on enzymes in the pancreas, the intestinal lumen and the intestinal mucosa. Pancreas weight per unit live weight showed a significant (P < 0.05) increase with increasing level of dietary tannin while that of the liver remained unaffected. Trypsin (EC 3.4.21.4) and α-amylase (EC 3.2.1.1) activities in the pancreas of birds fed at the highest level of tannins were more than double those from birds fed on a tannin-free control diet. In the intestinal lumen inhibition of trypsin activity increased with increasing level of dietary tannin; α-amylase activity was inhibited at intermediate tannin levels but was restored at the highest level. Dipeptidase (EC 3.4.13.11) and sucrose α-glucosidase (disaccharidase) (EC 3.2.1.48) in the intestinal mucosa were both inhibited by tannins. Growth of the birds and digestibility of nitrogen were adversely affected by the tannin-containing diets.

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Amino acid requirements of growing pigs 2. Identification of the limiting amino acid(s) in a low-protein diet supplemented with lysine

Animal Science ◽

10.1017/s0003356100025241 ◽

1981 ◽

Vol 33 (1) ◽

pp. 87-97 ◽

Cited By ~ 9

Author(s):

A. J. Taylor ◽

D. J. A. Cole ◽

D. Lewis

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Growth Performance ◽

Crude Protein ◽

Control Diet ◽

Live Weight ◽

Basal Diet ◽

Growing Pigs ◽

Carcass Composition ◽

Limiting Amino Acid

ABSTRACTA basal diet containing 120 g crude protein per kg and 9g lysine per kg, and previously shown to be limiting in one or more essential amino acids and/or non-essential nitrogen, was examined. It was fed either alone to growing female pigs from 25 kg to 55 kg live weight or in combination with four supplements of synthetic amino acids each containing three out of isoleucine, methionine, threonine and tryptophan. A control diet containing 140 g crude protein per kg and 9g lysine per kg was also included. Blood samples were collected at 40 kg live weight in order to examine the influence of dietary treatments on blood metabolites. Results for growth performance, carcass composition and blood urea indicated that threonine was the first limiting amino acid in the basal diet. Plasma free amino acids gave no clear trend. Growth performance and carcass composition were unaffected by supplementation of the diet with glycine indicating that the dietary supply of non-essential nitrogen was adequate.

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Effects of Herbal Adaptogen Feed-Additive on Growth Performance, Carcass Parameters, and Muscle Amino Acid Profile in Heat-Stressed Modern Broilers

Frontiers in Physiology ◽

10.3389/fphys.2021.784952 ◽

2021 ◽

Vol 12 ◽

Author(s):

Elizabeth S. Greene ◽

Clay Maynard ◽

Casey M. Owens ◽

Jean-François Meullenet ◽

Sami Dridi

Keyword(s):

Body Weight ◽

Heat Stress ◽

Amino Acid ◽

Growth Performance ◽

Feed Intake ◽

Control Diet ◽

Amino Acid Profile ◽

Poultry Production ◽

Carcass Parameters ◽

Cyclic Heat

Heat stress has strong adverse effects on poultry production and, thereby, threats its sustainability, which energized scientists to search for innovative and effective solutions. Here, we undertook this study to evaluate the effects of in-feed herbal adaptogen (stress response modifier) supplementation on growth performances, meat quality, and breast amino acid profile in chronic cyclic heat-stressed broilers. Day-old male Cobb 500 chicks (n = 720) were randomly assigned, in environmental chambers (n = 12, 24 pens), to three diet-treatments: a three-phase corn-soybean based diet fed as such (Control, C), or supplemented with the herbal adaptogen at 500 g/1000 kg control diet (NR-PHY-500) or at 1 kg/1000 kg control diet (NR-PHY-1000). From d29 to d42, birds from 9 chambers were exposed to cyclic heat stress (HS, 35°C from 9:30 am-5:30 pm), however, the rest of the chamber were maintained at thermoneutral conditions (24°C, TN), which creates 4 experimental groups: C-TN, C-HS, NR-PHY-500HS, and NR-PHY-1000HS (6 pens/group, 168 birds/group). HS altered growth performance via depression of feed intake and body weight. Adaptogen supplementation stimulated feed intake and averaged 65.95 and 83.25 g better body weight and 5 and 10 points better FCR at low and high dose, respectively, compared to heat-stressed birds. This increase in body weight was mirrored in enhanced weights of body parts (breast, tender, wings, and legs). Adaptogen supplementation modulated also breast amino acid profile, pH, color, and quality. Together, these data suggested that adaptogen supplementation could be a promising solution to alleviate heat stress, however further in-depth investigation for its mode of action and its underlying mechanisms are warranted.

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Effect of dietary inclusion levels of mechanically extracted neem seed cake on performance of young rabbits

Nigerian Journal of Animal Production ◽

10.51791/njap.v32i2.1067 ◽

2021 ◽

Vol 32 (2) ◽

pp. 233-239

Author(s):

G.S. Bawa ◽

M. Orunmuyi ◽

O.A. Onabanjo

Keyword(s):

Growth Performance ◽

Soybean Meal ◽

Control Diet ◽

Carcass Characteristics ◽

Live Weight ◽

Young Rabbit ◽

Neem Seed ◽

Weight Changes ◽

Feeding Trial ◽

Seed Cake

A feeding trial lasting 56 days was conducted using forty (40) weaned rabbits of mixed breeds with uncrage initial live weight of 669+20g to determine the effect of dietary inclusion levels of mechanically extracted neem seed cake on their performance and carcass characteristics. The animals were assigned lo five treatments based on their initial live weight and ser. There were eight individually caged rabbits per treatment. The diets were formulated to be isonitrogenous (20% CP). The control diet was based on maize and soybean meal. The neem seed cake was included at 0, 10, 20, 30 and 40% levels. Feed and wuler were offered ad libitum. Weekly feed intake and body weight changes were determined and feed efficiency calculated. At the end of the 56-day experiment, 5 rabbits per treatment were removed and slaughtered for carcass evaluation. The results showed that rabbits fed the 10 or 20% dietary levels of mechanically extracted neem seed cake hud growth performance and carcass characteristics that were statistically comparable (P>0.05) to those fed soybean meal (control) diet. Further increase in the dietary inclusion levels of the neem seed cake up to 30 or 40% resulted in significantly (P<0.05) depressed growth performance. The results favour the use of mechanically extracted neem seed cake in young rabbit diet up to the level of 20%.

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403 Using ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers: growth performance and antimicrobial resistance

Journal of Animal Science ◽

10.1093/jas/skz258.334 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 162-163

Author(s):

YiZhao Shen ◽

Tao Ran ◽

Atef Saleem ◽

Claudia Narveaz ◽

Walaa Gomaa ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Antimicrobial Resistance ◽

Growth Performance ◽

Control Diet ◽

Average Daily Gain ◽

Random Effect ◽

Linear Increase ◽

Beef Steers ◽

Fermentation Products ◽

Daily Gain

Abstract The objectives of this study were to assess the effects of Saccharomyces cerevisiae fermentation products (SCFP; NaturSafe® and Original XPC™, Diamond V) on growth performance and antimicrobial resistance in beef steers. Ninety Angus steers (initial BW=533±9.8 kg) were assigned to a randomized complete design with 6 treatments: 1) control (10% barley silage and 90% barley concentrate, DM basis), 2–4) control diet with 12, 15 and 18 g/d NaturSafe, 5) control diet with 8 g/d encapsulated XPC (eXPC), and 6) control diet with antibiotics (ANT; 330 mg/d monensin and 110 mg/d tylosin). Data were analyzed using the MIXED procedure of SAS with the fixed effect of treatment and random effect of steers. Though the final BW (715 kg) and DMI (12.0 kg/d) were not affected by treatments, average daily gain tended (P < 0.10) to be greater in steers supplemented with eXPC compared with control (1.80 vs. 1.62 kg/d) with a trend (P < 0.09) for a linear increase in gain: feed with increasing NaturSafe. The proportion of total abscessed liver did not differ among treatments (58.9%), but the proportion of severely abscessed liver tended (P < 0.10) to be lower in cattle supplemented with 15 and 18 g NaturSafe/d, eXPC and antibiotics (20.0, 20.0, 20.0 and 6.7 vs. 53.3%). The percentage of erythromycin resistant and erythromycin + tetracycline resistant Enterococci increased (P < 0.05) in steers receiving antibiotics compared with control and SCFP group (34.57 vs. 4.28 and 6.88%), while no difference was observed between control and SCFP groups. These results indicate that NaturSafe and eXPC were beneficial in improving the growth performance by increasing average daily gain and feed efficiency. Both NaturSafe and eXPC could potentially act as alternatives to growth promoting antibiotics.

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Effect of diet supplementation with live yeast Saccharomyces cerevisiae on growth performance, caecal ecosystem and health of growing rabbits

World Rabbit Science ◽

10.4995/wrs.2016.3991 ◽

2016 ◽

Vol 24 (3) ◽

pp. 191 ◽

Cited By ~ 3

Author(s):

T. Belhassen ◽

A. Bonai ◽

ZS. Gerencsér ◽

ZS. Matics ◽

T. Tuboly ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Weight Gain ◽

Growth Performance ◽

Feed Intake ◽

Clinical Chemistry ◽

Control Diet ◽

Temporary Increase ◽

Yeast Saccharomyces Cerevisiae ◽

Caecal Microbiota ◽

Live Yeast

<p>The aim of this study was to determine the effect of the live yeast Saccharomyces cerevisiae on the growth performance, caecal ecosystem and overall health of growing rabbits. A control diet was formulated (crude protein: 15.9%; neutral detergent fibre: 31.6%) and another diet obtained by supplementing the control diet with 1 g of Saccharomyces cerevisiae (6.5×109 colony-forming units) per kg of diet. Ninety 35-d old rabbits were allotted into 3 groups: TT (rabbits offered the supplemented diet from 17 d of age onwards), CT (rabbits offered supplemented diet from 35 d) and CC (rabbits fed non-supplemented diet). Body weight (BW) and feed intake were measured weekly and mortality was controlled daily. At 35, 42 and 77 d of age, 6 rabbits from each group were slaughtered and digestive physiological traits, serum clinical chemistry parameters, fermentation traits, and the composition of caecal microbiota examined. At 42 and 56 d of age, 10 rabbits from each group were injected intraperitoneally with 100 μg/animal of ovalbumin and blood samples were collected for examination of plasma immunological parameters. Throughout the experiment (5-11 wk), weight gain and feed intake (37.8 and 112.6 g/d, on av.) were not affected by yeast, except for weight gain in the first week after weaning, which was the highest in TT animals among the 3 groups (48.1 vs. 43.9 and 44.2 g/d for TT, CC and CT, respectively; P=0.012). This may be due to the increased trend in feed intake (P=0.072) in the TT group (96.4 g/d) compared to the others. Mortality (5/90) was low and did not differ among the 3 groups. Treatments had no effect on slaughter traits at the 3 sampling dates (35, 42 and 77 d). Only the weight of the empty caecum (% BW) was higher (P=0.02) in CC (2.2%) and CT (2.3%) than in TT group (1.8%) at 77 d of age. Treatments did not overtly affect the caecal microbiota, although the number of total anaerobic bacteria and Bacteroides were lower (108 and 107/g caecal digesta, respectively) in rabbits from CC group compared to those of CT and TT groups at 42 d of age (P=0.03). No difference between groups was observed for caecal short chain fatty acids profile, blood traits, or IgG and cytokine profile. In conclusion, supplementation of feed with yeast did not modify growth traits and resulted in only a temporary increase in weight gain and a slightly altered caecal microbiota after weaning.</p>

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A note on the growth and carcass characteristics of castrated male pigs fed on a ration containing diethylstilboestrol and methyltestosterone

Animal Science ◽

10.1017/s0003356100010953 ◽

1972 ◽

Vol 14 (2) ◽

pp. 255-257 ◽

Cited By ~ 2

Author(s):

N. Walker

Keyword(s):

Growth Performance ◽

Control Diet ◽

Subcutaneous Fat ◽

Carcass Characteristics ◽

Live Weight ◽

Hormone Treatment ◽

Backfat Thickness ◽

Effect On Growth

SUMMARYA control diet was compared with a diet containing 2 mg diethylstil-boestrol (DES) plus 2 mg methyltestosterone (MT) per kg air-dry feed, the hormone treatment extending either from 20 to 84 kg live weight or from 50 to 84 kg live weight. The experiment contained 20 blocks, each of 3 littermate castrated male pigs individually penned and fed to a restricted scale. Hormone supplementation had no effect on growth performance and significantly affected car-cass characteristics only when given for the longer period. Killing-out percentage, backfat thickness and percentage subcutaneous fat plus skin in the gammon joint were reduced and carcass length, percentage bone and percentage lean in the gammon joint were increased when the supplement was given over the longer period.

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