Weight and Lenght of Digestive Organs of Super Native Chickens Fed of Amorphophallus companulatus Tuber Flour

The purpose of this study was to examine the use of Amorphophallus companulatus (AC) tuber flour in feed on the weight and length of the digestive organs of super-native chickens. This research was conducted for 8 weeks. The experimental design used was a completely randomized design (CRD) with 4 treatments and 5 replications (P0 = feed without AC tuber flour, P1 = feed containing 5% AC tuber flour, P2 = feed containing AC tuber flour 7.5%, and P3 = feed containing 10% AC tuber flour). The results showed that the use of AC tuber flour in the super free-range chicken feed had a very significant effect (P<0.01) on the weight and length of the small intestine and significantly (P<0.05) on pancreatic weight, but had no effect (P>0 .05) against gizzard. It was concluded that AC bulbs could be used up to 10% in super-free-range chicken feed. the use of AC tuber flour as much as 7.5% in super free-range chicken feed resulted in the highest intestinal weight, intestinal length, and pancreas weight.

A 104-bp Structural Variation of the ADPRHL1 Gene Is Associated With Growth Traits in Chickens

Analyzing marker-assisted breeding is an important method utilized in modern molecular breeding. Recent studies have determined that a large number of molecular markers appear to explain the impact of “lost heritability” on human height. Therefore, it is necessary to locate molecular marker sites in poultry and investigate the possible molecular mechanisms governing their effects. In this study, we found a 104-bp insertion/deletion polymorphism in the 5′UTR of the ADPRHL1 gene through resequencing. In cross-designed F2 resource groups, the indel was significantly associated with weight at 0, 2, 4, 6, and 10 weeks and a number of other traits [carcass weight (CW), semi-evisceration weight (SEW), evisceration weight (EW), claw weight (CLW), wings weight (DWW), gizzard weight (GW), pancreas weight (PW), chest muscle weight (CMW), leg weight (LW), leg muscle weight (LMW), shedding Weight (SW), liver rate (LR), and leg muscle rate (LMR)] (P &lt; 0.05). In brief, the insertion-insertion (II) genotype was significantly associated with the greatest growth traits and meat quality traits, whereas the values associated with the insertion-deletion (ID) genotype were the lowest in the F2 reciprocal cross chickens. The mutation sites were genotyped in 4,526 individuals from 12 different chicken breeds and cross-designed F2 resource groups. The II genotype is the most important genotype in commercial broilers, and the I allele frequency observed in these breeds is relatively high. Deletion mutations tend to be fixed in commercial broilers. However, there is still considerable great potential for breeding in dual-purpose chickens and commercial laying hens. A luciferase reporter assay showed that the II genotype of the ADPRHL1 gene possessed 2.49-fold higher promoter activity than the DD genotype (P &lt; 0.05). We hypothesized that this indel might affect the transcriptional activity of ADPRHL1, thereby affecting the growth traits of chickens. These findings may help to elucidate the function of the ADPRHL1 gene and facilitate enhanced reproduction in the chicken industry.

Blood chemistry and relative organ weights of rabbits fed neem leaf meal based diets

Two experiments were conducted to determine the effect of neem leaf meal (NLM) based diets on blood and relative organ weight characteristics of rabbits. In the first experiment, 36 rabbit bucks aged 7 to 8 months were distributed into four dietary groups, T1, T2, T3 and T4 of 9 rabbits using completely randomized design. Each group was replicated 3 times with 3 rabbits per replicate. Rabbits in T1 group were fed diet that contained 0% NLM while those in T2 T3 and T4 groups were fed diets that contained NLM at 5%, 10% and 15%, respectively for 112 days. In the second experiment, 36 rabbit does were used and experimental procedures were laid out as described in experiment 1. Blood samples for analysis, live weight and organ weights were collected from each replicate and data obtained analysed statistically. Higher (p<0.05) kidney weight was obtained for rabbit does on diets T 2 T 3 and T4. Bucks on diet T2, recorded significantly (p<0.05) higher pancreas weight than those on diet T1 diet. No significant effect (p>0.05) on haematological parameters was observed among all treatments except for MCV and neutrophils which were significantly (p<0.05) decreased in the groups fed diets T2 and T4 and diets T1 and T3 groups respectively than others. Rabbit bucks and does on diet I, recorded significantly (p<0.05) higher serum cholesterol values than those on the other diets. Serum glucose value of rabbits on diet T, was significantly (p<0.05) different from those on the other diets. Serum ALP value of rabbits on diet T4 differed significantly (p<0.05) from those on the other 3 diets with the exception of bucks on diet T4 that had similar value with those on diet T1. Addition of neem leaf meal in rabbit diets increased serum AST and ALT values but the differences were not significant (p>0.05). It was therefore concluded that up to 5% of neem leaf meal can be included in the diet of pubertal rabbits without any deleterious effects on their organs and blood characteristics.

The Internal Organs Weight of 6-Weeks Old Native Chickens after Supplement Addition With L-threonine and L-tryptophan in the Feed

The aim of this experiment was to identify the internal organs weight of 6-weeks old native chickens after treatment feed with l-threonine and l-tryptophan supplement. This experimental research used 128 native chickens aged one-week-old in a Completely Randomized Design with four treatments and four replications. The dietary treatments were T0 (control feed), T1 (T0 + 0.35% l-threonine + 0.10% l-tryptophan), T2 (T0 + 0.68% l-threonine + 0.17% l-tryptophan), and T3 (T0 + 1.00% l-threonine + 0.25% l-tryptophan). The variables measured included body weight, liver weight, pancreas weight, gizzard weight, and intestinal length. Statistical analysis showed that l-threonine and l-tryptophan significantly affected body weight, liver weight, pancreas weight, gizzard weight and intestinal length. Supplementing 1.00% l-threonine and 0.25% l- tryptophan to feed contributed to the highest body weight and internal organs weight of native chickens.

Outcomes of Islet Autotransplantation in Chronic Pancreatitis Patients with Complete Acinar Atrophy

Total pancreatectomy with islet autotransplantation (TPIAT) is a promising treatment for refractory chronic pancreatitis (CP). Pathological features of CP include progressive fibrosis in pancreas parenchyma, atrophy, and/or ductal occlusion. Complete acinar atrophy (CAA) caused by chronic fibrosis and necroinflammation results in exocrine sufficiency and may influence islet isolation characteristics during TPIAT. In this analysis of patients who underwent TPIAT at our center, we compared transplant outcomes among those with CAA ( n = 5) vs non-acinar atrophy (NAA; matching controls, n = 36). Data were analyzed using one-way analysis of variance with Bonferroni post hoc test or Student’s t test. Pancreas digestion was longer in CAA than in NAA cases (18.6 vs 14.6 min) despite a lower pancreas weight (55.2 vs 91.2 g). Obtained tissue volume was 1.0 ml in the CAA group and 12.1 ml in the NAA group. Both groups had similar islet viability (96%) and islet dose (CAA, 3,391 IEQ/kg; NAA, 4141.1 IEQ/kg). During islet infusion, serum cytokine (IL-6, IL-8, and MCP-1) levels and plasma hsa-miR-375 levels were lower in the CAA group than in the NAA group, but not significantly. Serum tumor necrosis factor α levels at 3 h after infusion were significantly higher in CAA group than in NAA group. After TPIAT, the metabolic outcomes of the CAA group were comparable with that of the NAA group. Narcotics usage decreased significantly over 24 months in both groups, with the CAA group reporting being pain free at 12 months. Complete atrophy of acinar cells of pancreas did not significantly impact islet yield or endocrine function after TPIAT.

Excellent Islet Yields after 18-h Porcine Pancreas Preservation by Ductal Injection, Pancreas Preservation with MK Solution, Bottle Purification, and Islet Purification Using Iodixanol with UW Solution and Iodixanol with MK Solution

Successful islet isolation is the key to successful islet transplantation. Our group recently modified the islet isolation protocol to include pancreatic ductal injection of the preservation solution, pancreas storage in modified extracellular-type trehalose-containing Kyoto (MK) solution, and use of an iodixanol-based purification solution and bottle purification. In this study, we applied these methods to porcine islet isolation after 18-h pancreas preservation and compared two solutions with different compositions in bottle purification. Islet yield before purification was 651,661 ± 157,719 islet equivalents (IE) and 5576 ± 1538 IE/g pancreas weight. An IU solution was made by adding iodixanol to University of Wisconsin solution and an IK solution was made by adding iodixanol to MK solution. The efficacy of the two solutions for islet isolation was compared. There were no significant differences between the two purification methods with regard to islet yield, survival rate, purity, score, or stimulation index. These results indicate that our isolation protocol produces efficient islet yields from prolonged cold-stored pancreas and that IU and IK solutions are equally useful for islet purification.

KARATERISTIK ORGAN BAGIAN DALAM AYAM BURAS YANG DIBERI PAKAN MINYAK KELAPA (Cocos nucifera) DALAM RANSUM

INTERNAL ORGANS CHAR ACTERISTICS OF NATIVE CHICKEN FED BY COCONUT OIL (Cocos nucifera) ON DIET. The research was carried to determine the internal organs characteristics of buras chickens fed coconut (Cocos mucifera) oil in diet. A total 100 unsexed buras chickens was used in this experiment. The design used in this study was a completely randomized design (CRD) consisting of 5 treatments and 5 replications (4 hens each). The data were subjected to analysis of variance, when the treatments indicated significant effect it was continued Duncan’s Multiple Range Test. Five dietary treatments containing 0, 0.5%, 1 %, 1,5%, and 2% levels of coconut oil (CO) with five replicates were applied to chickens. Parameters measured were body weight, heart, liver, pancreas and gizzard weight. Result showed that CO in the ration significantly increased the body weight (P<0.01) but did not affect to heart weight, liver weight, pancreas weight and gizzard weight.(P>0.05) It can be concluded that coconut oil in the diet can’t increase the internal organ characteristics. We can gave the 2% CO in the diet for the best results. Key words: Internal Organs, Coconut Oil, Buras Chickens

PENGARUH SUPLEMENTASI DL-METIONIN DAN L-LISIN HCL PADA PAKAN STANDAR KAFETARIA TERHADAP BERAT BADAN, ORGAN DALAM DAN ORGAN REPRODUKSI AYAM KAMPUNG FASE PULLET

The aim of this study was to determine the effect of the supplementation of dl-methionine and l-lysine HCl to diet based on cafeteria standards of native chickens on body weight, internal organs and reproductive organs of native chicken at pullet phase. A total of 128 fourteen weeks-old native chickens were used in this study. The native chickens were divided into 4 treatments with 4 replications. The dietary treatments were: T0 (ration based on cafeteria standard), T1 (ration based on protein standard of NRC), T2 (cafeteria ration+0.09% DL-Methionine+0.22% L-Lysine HCl), T3 (cafeteria ration+0.19% dl-methionine+0.42% l-lysine HCl). The data collected were body weight, liver and bile, pancreas, gizzard, testicular and ovary weight of native chickens aged 20 weeks. The result of this study for T0, T1, T2 and T3 showed that body weight of chickens were 1418.60; 1431.59; 1503.88 and 1556.41 g/bird, liver and bile weights were 22.44; 21.79; 24.49 dan 25.93 g/bird, pancreas weights were 2.33; 2.28; 2.50 and 2.70 g/bird, gizzard weight were 25.31; 23.91; 25.38 and 26.86 g/bird, testicular weight were 3.66; 3.86; 9.55 and 9.46 g/bird, testicular volume were 3.81; 3.38; 9.13 and 9.16 and ovary weight were 1.12; 1.32; 3.14 and 4.61 g/bird, respectively. The results showed that supplementation of DL-Methionine and L-Lysine HCl gave significantly effect (P<0.05) upon body weight, liver and bile weight, pancreas weight, testicular weight and volume and ovary weight, but it did not effected to gizzard weight. It can be concluded that supplementation of 0.27% dl-methionine and 0.79% l-lysine HCl increase body weight, internal organs weight and reproductive organs weight of native chickens. Keywords: Cafeteria, DL-Methionine, L-Lysine HCl, Native Chicken, Supplementation.

EFEKTIFITAS PENAMBAHAN TEPUNG RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb ) DAN TEMU PUTIH (Curcuma zedoria Rosc) DALAM RANSUM TERHADAP High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL) DAN BERAT ORGAN DALAM PADA AYAM BROILER

An experiment was conducted to examine the effectiveness of curcuma xanthorrhiza Roxb and curcuma zedoria Rosc flours added in Ration on High density lipoprotein (HDL), low density lipoprotein (LDL) and the viscera of broiler. Fifty-four of broiler CP 707® strain were used in this experiment and they were placed into cages (60cm x 45cm x 45cm), 3 birds per cage. There were 18 bulb lamp of 25 watt above each cages. Randomized completely design were used in this experiment; 3 treatments and 6 repetitions. The three treatments were R0 (ration without Curcuma xanthorrhiza Roxb and Curcuma zedoria Rosc flours ration with), R1 (ration with 2% of Curcuma xanthorrhiza Roxb flours added in ration, and R2 (2% of Curcuma zedoria Rosc flours). The blood HDL level of broilers in this experiment were 133,58mg/dl (R0), 113,33mg/dl (R2) and 109mg/dl (R1), respectively. The blood LDL level of broiler were 45,66mg/dl (R0), 56,33mg/dl (R2) and 64,66mg/dl (R1), respectively. By using Chi square test, it showed that, there was a relations of addition of 2% of Curcuma xanthorrhiza Roxb and Curcuma zedoria Rosc flours in ration with blood HDL level. However, there was not a relation of addition of 2% of Curcuma xanthorrhiza Roxb and Curcuma zedoria Rosc flour in ration with blood LDL level. In short, the addition of 2% of Curcuma xanthorrhiza Roxb and Curcuma zedoria Rosc flours in ration were not efectively increased the level of HDL and not efectively decreased the level of LDL in blood. Furthermore, the percentage of gizard weight, percentage of pancreas weight, percentage of small and large intestine weight of broiler in this experiment were not significantly different between treatments (P>0,05).