The Screening of Potassium- and Phosphate-Solubilizing Actinobacteria and the Assessment of Their Ability to Promote Wheat Growth Parameters

Soil fertility and plant nutrition require an adequate management of essential macronutrients such as potassium (K) and phosphorus (P), which are mandatory for plant development. Bioleaching of K and P bearing minerals improves their chemical weathering and increases the performance of the biofertilization strategies. In this study, in vitro and greenhouse experiments were carried out to investigate P and K solubilization traits of nine Actinobacteria (P13, P14, P15, P16, P17, P18, BC3, BC10, and BC11) under fertilization with rock phosphate (RP). K and P solubilization were evaluated on Alexandrov and NBRIP media containing mica and six RP samples, respectively. The actinobacterial strains were able to solubilize K in Alexandrov medium supplemented with RP. However, when soluble P was used instead of RP, only four strains of Actinobacteria (Streptomyces alboviridis P18–Streptomyces griseorubens BC3–Streptomyces griseorubens BC10 and Nocardiopsis alba BC11) solubilized K. The solubilization values of K ranged from 2.6 to 41.45 mg/L while those of P varied from 0.1 to 32 mg/L. Moreover, all strains were able to produce IAA, siderophore, HCN, and ammonia and significantly improved the germination rate and the vigor index of wheat. The pot experiments revealed that four strains (Streptomyces alboviridis P18, Streptomyces griseorubens BC3, Streptomyces griseorubens BC10, and Nocardiopsis alba BC11) significantly improved the growth parameters of wheat, namely root length (1.75–23.84%), root volume (41.57–71.46%), root dry weight (46.89–162.41%), shoot length (8.92–23.56%), and shoot dry weight (2.56–65.68%) compared to the uninoculated control. These findings showed that Streptomyces griseorubens BC10 and Nocardiopsis alba BC11 are promising candidates for the implementation of efficient biofertilization strategies to improve soil fertility and plant yield under rock P and rock K fertilization.

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Effects of Phosphate Solubilizing Fungi on Growth and Yield of Haricot Bean (Phaseolus vulgaris L.) Plants

Journal of Agricultural Science ◽

10.5539/jas.v8n10p204 ◽

2016 ◽

Vol 8 (10) ◽

pp. 204 ◽

Cited By ~ 1

Author(s):

Firew Elias ◽

Diriba Muleta ◽

Delelegn Woyessa

Keyword(s):

Phaseolus Vulgaris ◽

Dry Weight ◽

Growth And Yield ◽

Fresh Weight ◽

Shoot Height ◽

Shoot Dry Weight ◽

Standard Procedures ◽

Bean Plants ◽

Phosphate Solubilizing

Haricot bean (Phaseolus vulgaris L.) is one of the most important cash crops and export commodities besides its use in human food and soil fertility improvement. Phosphorus (P) is one of the major bio-elements that limits agricultural production. However, phosphate-solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. The purpose of this study was to evaluate the effects phosphate solubilizing fungi on the growth of haricot bean plants. Cultural and morphological features were used to tentatively identify the fungal isolates to genus level. Based In vitro phosphate solubilization efficieny conducted in both solid and liquid PVK medium following standard procedures, two best isolates were selected and evaluated under greenhouse for their performance on haricot bean. Under greenhouse experiment, shoot height (47.31 cm plant-1), root length (41.01 cm plant-1), nodule number (65.67 plant-1), nodule dry weight (0.59 g plant-1), shoot fresh weight (62.73 g plant-1), shoot dry weight (14.33 g plant-1), number of pod (12.89 plant-1), 50-seed weight (35.89 g plant-1), P content (0.59%) and N content (1.96%) were significantly increased by co-inoculation of two isolates (PSFAP) in the soil amended with rock phosphate (RP) compared to control. Moreover, the highest number of leaves (59.55 leaves plant-1) and root fresh weight (14.19 g plant-1) were recorded as a result of inoculation with isolate PSFP compared to control. The present study indicated the presence of potential plant associated fungi that possess phytobeneficial traits for extending their use as microbial biofertilizers after testing their suitability for the desired purpose.

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EFFECT OF MINERAL DEFICIENCY ON MORPHOLOGICAL AND PHYSIOLOGICAL PARAMETERS AND ON SHOOT AND ROOT MINERAL PARTITIONING IN THREE SUNFLOWER VARIETIES / EFECTO DE LA FALTA DE ELEMENTOS MINERALAS SOBRE LOS PARAMENTROS MORFOLOGICOS Y FISIOLOGICOS Y REPARTICION DE ELEMENTOS MINERALES DE LA PARTE SOBRE EL SUELO Y DE LA RAIZ EN TRES VARIEDADES / INFLUENCE DE LA CARENCE D’ÉLÉMENTS MINÉRAUX SUR LES PARAMÈTRES MORPHOLOGIQUES ET PHYSIOLOGIQUES ET SUR LA DISTRIBUTION DES ÉLÉMENTS MINÉRAUX ENTRE LA PARTIE AÉRIENNE ET LA RACINE POUR TROIS SORTES DE TOURNESOL

Helia ◽

10.1515/helia.2001.24.35.135 ◽

2001 ◽

Vol 24 (35) ◽

pp. 135-148

Author(s):

Mohammed El Midaoui ◽

Ahmed Talouizte ◽

Benbella Mohamed ◽

Serieys Hervé ◽

Ait Houssa Abdelhadi ◽

...

Keyword(s):

Leaf Area ◽

Dry Matter ◽

Growth Parameters ◽

Dry Weight ◽

Mineral Deficiency ◽

P Deficiency ◽

Plant Parts ◽

Shoot Dry Weight ◽

K Deficiency ◽

Matter Effect

SUMMARYAn experiment has been carried out in order to study the behaviour under mineral deficiency of three sunflower genotypes, a population variety (Oro 9) and two hybrids (Mirasol and Albena). Sunflower seedlings were submitted to five treatments: N deficiency (N0), P deficiency (P0), K deficiency (K0), N and K deficiency (N0K0) and a control. Plants were harvested when they reached 3-4 true pairs of leaves. Growth parameters measured (height, total leaf area, root length, root and shoot dry mater) were all significantly reduced by mineral deficiency. Leaf area was most reduced by N0 (-61%) and P0 (-56%). Total dry matter was most affected by N0 (-63%) and by N0K0 (-66%). Genotype comparisons showed that Oro 9 had the highest shoot dry matter while Albena had the lowest root dry matter. Effect of mineral deficiency on content and partitioning of N, P, K, Ca and Na was significant and varied according to treatments and among plant parts. Shoot dry weight was significantly correlated with root N content (r2=0.81) and root K content (r2=-0.61) for N0 and K0.

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Applications of plant growth promoting bacteria and Trichoderma spp. for controlling Orobanche crenata in faba bean

Bulletin of the National Research Centre ◽

10.1186/s42269-019-0263-y ◽

2020 ◽

Vol 44 (1) ◽

Cited By ~ 1

Author(s):

Mahmoud Ahmed Touny El-Dabaa ◽

Hassan Abd-El-Khair

Keyword(s):

Plant Growth ◽

Faba Bean ◽

Growth Parameters ◽

Dry Weight ◽

Research Centre ◽

Plant Growth Promoting Bacteria ◽

Fresh Weight ◽

Orobanche Crenata ◽

Trichoderma Spp ◽

Shoot Dry Weight

Abstract Background Orobanche crenata is an obligate root parasite belonging to Orbanchaceae. Broomrape causes great damage to the faba bean. Several attempts were applied for controlling parasitic weeds. So, the aim of this work is to study the application of Trichoderma spp. as well as three rhizobacteria species in comparison to herbicidal effect of Glyphosate (Glialka 48% WSC) for controlling broomrape infesting faba bean (Vicia faba). Materials and methods Three pot experiments were carried out in the greenhouse of the National Research Centre, Dokki, Giza, Egypt during two successive winter seasons. Trichoderma inocula were adjusted to 3.6 × 108 propagules/ml and the bacterium inocula were adjusted at 107–109 colony-forming unit (CFU)/ml. All treatments were applied, before 1 week of sowing, at rate of 50 ml per pot in experiments I and II, while 100 ml per pot in experiment III. Results Trichoderma spp. (T. harzianum, T. viride and T. vierns) as well as three rhizobacteria species (Pseudomonas fluorescens, Bacillus subtilis and Bacillus pumilus) enhanced the growth parameters in faba bean plants, i.e. shoot length, shoot fresh weight, shoot dry weight and leaf number in the first experiment when applied without O. crenata infection. In the second experiment, all bio-control could protect plants against O. crenata infection, where it had better juvenile number reduction, than glyphosate after 2 months of application. Both B. subtilis and B. pumilus had the highest reduction to juvenile fresh weight, while their effect was equal to herbicide for juvenile dry weight, respectively. The bio-control agents had high effects until the 4th month, but it was less than that of the herbicide. In experiment III, the bio-control agents could highly reduce the juvenile parameters after 2 months, as well as juvenile fresh weight and juvenile dry weight after 4 months, than the herbicide, respectively. The bio-control agents were effective until 6 months, but less than the herbicide effect. All bio-control treatments highly increased the plant growth parameters, than the herbicide. Conclusion The application of Trichoderma spp. as well as rhizobacteria species could play an important role in controlling broomrape in faba bean as a natural bioherbicide.

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MODIFICAÇÕES NO MEIO DE CULTURA, FOTOPERÍODO E TEMPO DE CULTIVO AFETAM O ALONGAMENTO E ENRAIZAMENTO IN VITRO DE BANANEIRA CV. PACOVAN

Nativa ◽

10.31413/nativa.v6i1.4731 ◽

2018 ◽

Vol 6 (1) ◽

pp. 27

Author(s):

Marcos Vinícius Marques Pinheiro ◽

Ana Cristina Portugal Pinto De Carvalho ◽

Fabrina Bolzan Martins

Keyword(s):

Plant Height ◽

Culture Medium ◽

Culture Media ◽

Dry Weight ◽

Salt Mixture ◽

Musa Spp ◽

Fresh Biomass ◽

Shoot Dry Weight ◽

Number Of Leaves

No intuito de elevar as taxas de sobrevivência durante a etapa de aclimatização e posterior plantio a campo, avaliou-se o enraizamento in vitro de bananeira cv. Pacovan, em diferentes concentrações de sais MS e de sacarose. Utilizou-se DIC, esquema fatorial (6x2x3), com seis meios de cultura [sendo três concentrações de nutrientes do meio MS (100%; 50% de macronutrientes; e 50% dos sais macro e micronutrientes), e duas concentrações de sacarose (1,5/3,0%)], dois fotoperíodos (12/16 h) e três tempos de cultivo (21, 28 ou 35 dias) e seis repetições/tratamento. Analisaram-se: altura da planta, número de folhas/planta, massas frescas e secas das partes aérea e radicular. Para altura da planta, massa fresca da parte aérea e radicular, o meio MS 50% dos sais + sacarose (1,5%) com fotoperíodo de 16 h e tempo de cultivo de 35 dias foi satisfatório. Para massa seca da parte aérea foi MS 50% de sais + sacarose (3%), e para massa seca da parte radicular, MS 100% + sacarose (3%) (em 12hs/28 dias e 16hs/21 dias). Para o alongamento/enraizamento in vitro da bananeira cv. Pacovan sugere-se MS 50% de sais (macro e micronutrientes), redução ou manutenção de sacarose (1,5 ou 3%) em 16h/35 dias de cultivo.Palavra-chave: Musa spp., propagação in vitro, sistema radicular. CHANGES IN CULTURE MEDIUM, PHOTOPERIOD AND TIME OF CULTIVATION AFFECT THE IN VITRO ELONGATION AND ROOTING OF BANANA CV. PACOVAN ABSTRACT:In order to achieve high rates of survival during the acclimatization and later planting in the field, was evaluated the in vitro of banana cv. Pacovan plants under different concentrations of sucrose and MS basal salt mixture. The experiment was assembled in a DIC, in 6x2x3, six different culture media [three different MS salt mixture concentrations (100%; 50% of macronutrients; and 50% of macro/micronutrients) and two sucrose concentrations (1.5/3%)], two photoperiods (12/16 hours) and three cultivation times (21, 28 or 35 days). Each treatment was composed by 6 replicates. Plant height, number of leaves/plant, fresh and dry weight of roots and shoots, were analyzed. Satisfactory results for plant height and shoot and root fresh biomass were observed in MS with macro/micronutrients (50%) + sucrose (3%), 16 hours/35 days. The highest values of shoot dry weight were observed in MS with macro/micronutrients (50%) + sucrose (3%); the highest root dry weight was achieved with MS 100% + sucrose (3%) (12hs/28 and 16hs/21 days). The suggested medium for the in vitro elongation and rooting stage of banana cv. Pacovan is the MS with 50% of salts (macro and micronutrients), reduction or maintenance of sucrose (1.5 or 3%) in 16h/35 days of cultivation.Keywords: Musa spp., in vitro propagation, root system. DOI:

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Isolation of Phosphate Solubilizing Bacteria and their Use for Plant Growth Promotion in Tomato Seedling and Plant

Kathmandu University Journal of Science Engineering and Technology ◽

10.3126/kuset.v13i2.21284 ◽

2018 ◽

Vol 13 (2) ◽

pp. 61-70

Author(s):

Rajiv Pathak ◽

Vipassana Paudel ◽

Anupama Shrestha ◽

Janardan Lamichhane ◽

Dhurva. P. Gauchan

Keyword(s):

Plant Growth ◽

Growth Promotion ◽

In Vitro Study ◽

Dry Weight ◽

Greenhouse Experiment ◽

Phosphate Solubilizing Bacteria ◽

Tomato Seedling ◽

Phosphate Solubilizing ◽

Phosphorous Compounds

Phosphorous (P) is an essential macronutrient and most soils contain high levels of P. However, its availability to plant is limited by rapid immobilization of phosphorous compounds to insoluble forms and hence plant available forms of P in soils are found in low amounts. Phosphate solubilizing bacteria provide an eco-friendly alternative to convert insoluble phosphates into plant available forms. In the present study, three phosphate solubilizing bacterial isolates (PB-1, PB-4 and VC-01) with visually significant phosphate solubilizing abilities were isolated from tomato rhizosphere soil. In-vitro study in pikovskaya’s agar revealed that isolate PB-1 had the highest phosphate solubilizing ability with a phosphate solubilizing index of 2.08±0.07 followed by isolate VC-01 (1.31±0.09) and PB-4 (1.24±0.08). Isolates were used as bacterial inoculum to assess their ability to promote tomato (Lycopersicon esculentum var. Srijana) seedling and plant growth in in-vitro and greenhouse experiment respectively. Isolate PB-4 showed best growth promotion in seedling assay whereas isolate PB-1 and VC-01 also promoted seedling growth compared to control. In greenhouse experiment however, isolates VC-01 and PB-1 significantly enhanced all parameters (shoot length, root length, shoot and root dry weight) compared to uninoculated control whereas isolate PB-4 had a positive effect on all parameters except root length.Kathmandu University Journal of Science, Engineering and TechnologyVol. 13, No. 2, 2017, page: 61-70

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Biohardening of Arabidopsis thaliana Seeds and Seedlings with Fraser Photinia Associated Bacterium (PGB_invit) in In vitro Conditions

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47311488 ◽

2019 ◽

Vol 47 (3) ◽

pp. 954-961

Author(s):

Maria BATOOL ◽

Özlem AKKAYA ◽

Mine GÜL ŞEKER ◽

Yelda ÖZDEN ÇİFTÇİ

Keyword(s):

Arabidopsis Thaliana ◽

Photosynthetic Pigments ◽

Growth Parameters ◽

Endophytic Bacterium ◽

Dry Weight ◽

Bacterial Populations ◽

Positive Effects ◽

Inactive Form ◽

Potential Possibility

The aim of this study was to analyze possible positive effects of putatively endophytic PGPB (PGB_invit), which was isolated from long-term in vitro cultured fraser photinia microshoots, on seed and 7-day old seedling stages of Arabidopsis thaliana. Seeds and in vitro-germinated seedlings were inoculated with 107 CFU/mL and 108 CFU/mL active (A) and inactive (I) endophytic bacterial populations along with their mix compositions (A+I) and suspended in MPYE broth together with their controls (untreated ones). 14 days old seedlings were evaluated for various plant growth parameters [i.e., shoot and root fresh weight (FW), shoot length (SL), shoot and root dry weight (DW), root length (RL) and photosynthetic pigments including chlorophyll a, b and carotenoids of plantlets] as well as endophytic and rhizospheric trait of bacteria. Positive effects of inactive and active bacterium on FW, DW and photosynthetic pigments for 7-day inoculated seedlings were recorded whereas an increase in photosynthetic contents for seed stage inoculations was observed. Rhizospheric and endophytic colonization of the bacteria was confirmed by PCR with the presence of virD1 gene, which is previously recorded to be existed in the plasmid of bacterium after inoculation. Overall, these results demonstrated that this peculiar putative endophytic bacterium being beneficial in active and even more useful in inactive form for A. thaliana when optimum conditions and concentrations are used. Moreover, presence of virD1 gene suggested its potential possibility to be used in bioengineering along with various other beneficial PGPR features as biofertilizer.

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IN VITRO DEVELOPMENT OF YELLOW LAPACHO (BIGNONIACEAE) USING HIGH-POWER LIGHT EMITTING DIODE

Revista Árvore ◽

10.1590/1806-90882018000500008 ◽

2018 ◽

Vol 42 (5) ◽

Author(s):

Ezequiel Enrique Larraburu ◽

Gonzalo Sanchez Correa ◽

Berta Elizabet Llorente

Keyword(s):

In Vitro Culture ◽

High Power ◽

Stomatal Density ◽

Light Emitting Diode ◽

Dry Weight ◽

Light Sources ◽

Multiplication Rate ◽

Light Emitting ◽

Shoot Dry Weight

ABSTRACT Handroanthus ochraceus (yellow lapacho) is a medicinal, ornamental and timber tree which can be propagated by in vitro culture. Conventional methods use fluorescent lighting (FL), whereas light emitting diode (LED) has been used for this purpose only recently. The aim of this work was to evaluate the effects of FL and high-power LED (HP-LED) on the in vitro multiplication and rooting of yellow lapacho at different irradiances (15 to 60 µmol m-2s-1). Epicotyls obtained from half-siblings was multiplicated in WPM (Woody Plant Medium) supplemented with 20 µM benzilaminopurine and 1 mM IBA (indolebutiric acid). For rooting, shoots were cultured for 3 days in ½WPM supplemented with 50 µM IBA and for 42 days in auxin-free ½WPM under HP-LED or FL lighting. Under HP-LED, the multiplication rate of shoots increased significantly (61%) from 20 to 40 µmol m-2s-1 respect to FL. Differences in abaxial stomatal density and size were observed between light sources at 20 µmol m-2s-1. High HP-LED irradiance produced the highest rooting percentage. In the rooting stage, the marginal means of treatments without factors interaction showed that HP-LED irradiances significantly increased shoot length by 20%, shoot fresh weight by 77% and shoot dry weight by 30% in comparison to the values under FL. The maximum values calculated from the regression curves were around 50 µmol m-2 s-1 for HP-LED for all parameters except root lenght whereas were around 20 µmol m-2 s-1 for FL for all parameters except fresh and dry weigth of shoot. Here we show that HP-LED lighting improve in vitro culture of H. ochraceus, reduced 81% energy consumption respect to FL and uses only a multispectral LED instead of different single color LEDs. Therefore, HP-LED could be useful for the micropropagation of tree species contributing to sustainable agriculture and ecological restoration of degraded areas.

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Growth response of pigeon pea (Cajanus cajan L. Millsp) on saline and alfisol soils

E3S Web of Conferences ◽

10.1051/e3sconf/202130601010 ◽

2021 ◽

Vol 306 ◽

pp. 01010

Author(s):

M. Soedarjo

Keyword(s):

Growth Retardation ◽

Saline Soil ◽

Growth Parameters ◽

Research Work ◽

Dry Weight ◽

Pigeon Pea ◽

Saline Soils ◽

Growth Responses ◽

High Concentration ◽

Shoot Dry Weight

As an archipelago country, Indonesia is surrounded by abundant saline soils which could be potential for growing food crops. The research work was conducted to study the growth responses of some pigeon pea genotypes on saline soils. The genotypes of Pigeon pea (LG Kidul, Mega, 90024, 91043 and 94028), as treatment, were grown on Alfisol soil (non-saline) and on saline soil. The experiment was carried out using completely randomized design and replicated three times. All data observed were analyzed by employing standard deviation from 3 replicates. The results of present research work revealed a significant growth retardation of pigeon pea when grown on saline soil. All growth parameters, plant height, plant diameter, root dry weight, and shoot dry weight were significantly lower on saline soil than on Alfisol soil. High concentration of Na+ was observed in roots, shoots and leaves of pigeon pea indicating growth retardation and toxicity symptoms of pigeon pea on saline soil was due to Na+.

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GIBBERELLIC ACID DURING PRIMING OF PURPLE CONEFLOWER [ECHINACEA PURPUREA (L) MOENCH.] SEEDS IMPROVES GERMINATION AND SEEDLING EMERGENCE

HortScience ◽

10.21273/hortsci.30.2.186c ◽

1995 ◽

Vol 30 (2) ◽

pp. 186c-186

Author(s):

Janine O. Haynes ◽

Wallace G. Pill

Keyword(s):

Gibberellic Acid ◽

Germination Rate ◽

Seedling Emergence ◽

Dry Weight ◽

Echinacea Purpurea ◽

Germination Percentage ◽

Shoot Dry Weight ◽

Greenhouse Study ◽

Purple Coneflower ◽

Percentage Points

Purple coneflower seeds following priming (-0.04 MPa, 10 days, 15C, darkness) osmotically in polyethylene glycol 8000 (PEG) or matrically in expanded no. 5 vermiculite had greater germination rate and synchrony at continuous 20C or 30C than untreated seeds, but germination percentage was unaffected. Inclusion of 5.5 × 10-2 M gibberellic acid (GA3 as ProGibb Plus 2X, Abbott Laboratories, N. Chicago, Ill.) further improved germination rate and synchrony at 20C, but not at 30C. In a greenhouse study (30C day/27C night, July-August natural light), seeds primed in PEG or vermiculite containing G A3 compared to untreated seeds had 6 percentage points higher maximum emergence (ME), 3.3 fewer days to 50% ME, 1.9 fewer days between 10% and 90% ME, 116% greater shoot dry weight, and 125% longer leaves at 16 days after planting in peat-lite. Inclusion of ethephon (0.01 m, as Florel) either alone or with GA3 during priming provided no benefit to seed germination or seedling emergence. Moistened vermiculite substituted for PEG solution as a priming medium for purple coneflower seeds, the priming benefit on seedling emergence and growth being enhanced by 5.5 × 10-2m G A3 inclusion in the priming media.

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Response of 10 Aster Species to Saline Water Irrigation

HortScience ◽

10.21273/hortsci.51.2.197 ◽

2016 ◽

Vol 51 (2) ◽

pp. 197-201 ◽

Cited By ~ 1

Author(s):

Shasha Wu ◽

Youping Sun ◽

Genhua Niu ◽

James Altland ◽

Raul Cabrera

Keyword(s):

Growth Parameters ◽

Saline Water ◽

Dry Weight ◽

Poor Quality ◽

Mineral Concentration ◽

Salt Damage ◽

Shoot Dry Weight ◽

Quality Water ◽

Poor Quality Water ◽

Copper Canyon

Asteraceae is one of the largest plant families with many important garden ornamental species. Salt tolerance of 10 aster perennials was evaluated in a greenhouse experiment, including the following: damianita (Chrysactinia mexicana), gregg’s mistflower (Eupatorium greggii), shasta daisy (Leucanthemum ×superbum ‘Becky’), blackfoot daisy (Melampodium leucanthum), lavender cotton (Santolina chamaecyparissus), aromatic aster (Symphyotrichum oblongifolium), copper canyon daisy (Tagetes lemmonii), four-nerve daisy (Tetraneuris scaposa), skeleton-leaf goldeneye (Viguiera stenoloba), and zexmenia (Wedelia texana). Plants were irrigated with nutrient solution at electrical conductivity (EC) of 1.2 dS·m−1 (control) or saline solutions at EC of 5.0 or 10.0 dS·m−1 (EC 5 or EC 10) for 5 weeks. Upon termination, growth parameters, foliar salt damage, relative chlorophyll content [Soil-Plant Analysis Development (SPAD) readings], and mineral concentration were measured. Gregg’s mistflower, skeleton-leaf goldeneye, and lavender cotton were the most salt-tolerant species with less reductions in shoot dry weight (DW) in both EC 5 and EC 10. Considering the relatively severe foliar salt damage (visual quality score of 3.1 and 2.7 at EC 5; 2.4 and 1.6 at EC 10) and mortality rate (10% and 40%) in EC 10, aromatic aster and zexmenia should be avoided where poor quality water may be used for irrigation. Gregg’s mistflower and skeleton-leaf goldeneye had relatively lower leaf sodium (Na) concentrations suggesting that both species can selectively exclude Na. Damianita and the four daisies, i.e., blackfoot daisy, copper canyon daisy, four-nerve daisy, and shasta daisy, were salt sensitive as evidenced by their greater growth reduction, foliar salt damage, and high Na and chlorine (Cl) accumulation in leaves, and should be avoided in landscapes where poor quality water may be used for irrigation.

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