scholarly journals Isolation and Characterization of Novel Bacteria Capable of Degrading 1,4-Dioxane in the Presence of Diverse Co-Occurring Compounds

2021 ◽  
Vol 9 (5) ◽  
pp. 887
Author(s):  
Tanmoy Roy Tusher ◽  
Takuya Shimizu ◽  
Chihiro Inoue ◽  
Mei-Fang Chien
Keyword(s):  
Industrial Wastewater ◽  
Cost Effective ◽  
Organic Load ◽  
Bacterial Strains ◽  
Gene Encoding ◽  
Initial Oxidation ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Single Carbon Source ◽  
Group 5

Biodegradation is found to be a promising, cost-effective and eco-friendly option for the treatment of industrial wastewater contaminated by 1,4-dioxane (1,4-D), a highly stable synthetic chemical and probable human carcinogen. This study aimed to isolate, identify, and characterize metabolic 1,4-D-degrading bacteria from a stable 1,4-D-degrading microbial consortium. Three bacterial strains (designated as strains TS28, TS32, and TS43) capable of degrading 1,4-D as a sole carbon and energy source were isolated and identified as Gram-positive Pseudonocardia sp. (TS28) and Gram-negative Dokdonella sp. (TS32) and Afipia sp. (TS43). This study, for the first time, confirmed that the genus Dokdonella is involved in the biodegradation of 1,4-D. The results reveal that all of the isolated strains possess inducible 1,4-D-degrading enzymes and also confirm the presence of a gene encoding tetrahydrofuran/dioxane monooxygenase (thmA/dxmA) belonging to group 5 soluble di-iron monooxygenases (SDIMOs) in both genomic and plasmid DNA of each of the strains, which is possibly responsible for the initial oxidation of 1,4-D. Moreover, the isolated strains showed a broad substrate range and are capable of degrading 1,4-D in the presence of additional substrates, including easy-to-degrade compounds, 1,4-D biodegradation intermediates, structural analogs, and co-contaminants of 1,4-D. This indicates the potential of the isolated strains, especially strain TS32, in removing 1,4-D from contaminated industrial wastewater containing additional organic load. Additionally, the results will help to improve our understanding of how multiple 1,4-D-degraders stably co-exist and interact in the consortium, relying on a single carbon source (1,4-D) in order to develop an efficient biological 1,4-D treatment system.

scholarly journals ISOLATION AND CHARACTERIZATION OF PHENOL DEGRADING BACTERIA FROM INDUSTRIAL WASTEWATER AND SEWAGE WATER

2021 ◽  
Vol 48 (2) ◽  
pp. 443-457
Author(s):  
Salma Salem ◽  
H. Abd El‌‌‌-Fattah ◽  
Howaida Abdelbasit ◽  
S. Mahgoub
Keyword(s):  
Industrial Wastewater ◽  
Sewage Water ◽  
Isolation And Characterization ◽  
Degrading Bacteria

scholarly journals Role of syntrophic microbial communities in high-rate methanogenic bioreactors

2012 ◽  
Vol 66 (2) ◽  
pp. 352-362 ◽  
Author(s):  
Alfons J. M. Stams ◽  
Diana Z. Sousa ◽  
Robbert Kleerebezem ◽  
Caroline M. Plugge
Keyword(s):  
Fatty Acid ◽  
Industrial Wastewater ◽  
Microbial Community Composition ◽  
Methanogenic Archaea ◽  
High Strength ◽  
Cost Effective ◽  
Anaerobic Treatment ◽  
High Rate ◽  
Degrading Bacteria

Anaerobic purification is a cost-effective way to treat high strength industrial wastewater. Through anaerobic treatment of wastewaters energy is conserved as methane, and less sludge is produced. For high-rate methanogenesis compact syntrophic communities of fatty acid-degrading bacteria and methanogenic archaea are essential. Here, we describe the microbiology of syntrophic communities in methanogenic reactor sludges and provide information on which microbiological factors are essential to obtain high volumetric methane production rates. Fatty-acid degrading bacteria have been isolated from bioreactor sludges, but also from other sources such as freshwater sediments. Despite the important role that fatty acid-degrading bacteria play in high-rate methanogenic bioreactors, their relative numbers are generally low. This finding indicates that the microbial community composition can be further optimized to achieve even higher rates.

scholarly journals Screening and optimization of arsenic degrading bacteria and their potential role in heavy metal bioremediation

2019 ◽  
Vol 35 (4) ◽  
Author(s):  
Mehwish Iqtedar ◽  
Farah Aftab ◽  
Rabab Asim ◽  
Roheena Abdullah ◽  
Afshan Kaleem ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Arsenic Concentration ◽  
Cost Effective ◽  
Maximum Growth ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
High Concentration ◽  
Arsenic Tolerance ◽  
Degrading Bacteria ◽  
Soil And Water

Industrialization has added extremely toxic metalloid arsenic into the environment which at high concentration severely threatens the biota. Naturally, some microbes possess the ability to bio-accumulate metals and also to transform arsenite (As III) a toxic form to a non-toxic arsenate As V. The present study aimed to isolate arsenic resistant bacterias from the arsenic contaminated soil and water. Among eleven bacterial isolates, three FAs 1, 4 and 9 exhibited tolerance against sodium arsenite at 100mM concentration by achieving growth of 7.48×109,1.57×109 and 2.23×109 C.F.U./ml, respectively. Optimization at different conditions such as temperature, pH and arsenic concentration revealed high arsenic tolerance from isolate FAs 4 (5.33×108) at 37°C and FAs 1 (4.43×108 C.F.U./ml) at pH 7. Arsenic resistance at optimum conditions for the bacterial strains FAs 1, FAs 4 and FAs 9 showed maximum growth at 80mM concentration of arsenite. These bacterial isolates did not show redox ability to oxidize arsenite As III to arsenate As V. However bacterial isolates FAs 1, FAs 4 and FAs 9 were able to accumulate arsenic 39.16, 148 and 125 µg/L on the 4th, 3rd and 5th day of incubation, respectively. The isolates FAs 1, FAs 4 and FAs 9 were identified as Gram negative non endospore forming rods. In future, these novel isolates possess a great potential in biotechnology field, as bioremediation of arsenic contaminated soil and water can be done by employing arsenic accumulating bacteria which is an eco-friendly and cost effective method.

scholarly journals Bioremediation of Petroleum Polluted Soils using Consortium Bacteria

2020 ◽  
pp. 961-969
Author(s):  
Dina Hasan Nafal ◽  
Hind Suhail Abdulhay
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Oxygen Demand ◽  
Cost Effective ◽  
Bacterial Consortium ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterial Strains ◽  
Degrading Bacteria ◽  
Oil Concentration ◽  
Kocuria Rosea ◽  
Physical And Chemical

      This study was carried out to isolate opportunistic hydrocarbons oil-degrading bacteria and develop a consortium or a mixture of bacteria with high biodegradation capabilities which can be used in biological treatment units of the contaminated water before release. The biological processes in general are environmentally friendly and cost effective, as they are easy to design and apply; as such they are more appropriate to the public.     The location of the study was in Al-Dora refinery sludge holes area. The samples were collected for three seasons (winter, spring and summer) each consisted of three months.  The sludge samples were analyzed for various physical and chemical parameters. Temperature values of the sludge were at maximum in summer season, reaching 32˚C, whereas they were at minimum in winter (24 ˚C). The values of sludge pH were at maximum in summer (9.70) and minimum in winter (9.20). Turbidity levels were 382 NTU in spring and 353 NUT in winter. Biological oxygen demand (BOD5) was at maximum in summer (760) and (690 mg/l) in winter. The maximum dissolved oxygen (DO) value of 5.20 mg/l was recorded in winter, while the minimum was 3.80 mg/l recorded in summer. The maximum electrical conductivity (EC) was 17130 μs/cm recorded in summer, while the minimum was 16150 μs/cm recorded in winter. The maximum total dissolved solids (TDS) values were 10335 mg/l recorded in summer, while the minimum (10015 mg/l) was recorded in winter. The maximum total petroleum hydrocarbon (TPH) value (431 mg/l) was recorded in summer, while the minimum (367 mg/l) was recorded in spring. Finally, the maximum salinity value (9.90%) was recorded in spring, while the minimum (9.30%) was recorded in winter. Also, hydrocarbon compounds in sludge samples were measured using Gas Chromatography - Mass Spectrometry (GC-MS), and the result showed that they were composed of 31 hydrocarbon compounds.In the present work, nineteen sludge degrading bacterial strains were isolated from the soil near Al-Dora refinery hole by primary and secondary screenings using a modified mineral salt medium supplemented with 1% (v/v) sludge as a carbon source. The most efficient two sludge degraded isolates identified by VITIK 2 compact were Kocuria rosea and Bacillus amyloliquefaciens. The tow isolates and there mixture showed best growth at 30°C for 12 days, as shown by the measurement of the optical density of the liquid culture and the final oil concentration by spectrophotometer.      The bacterial isolates in liquid media with 2% (v/v) sludge showed best growth and the maximum biodegradation percentage after 12-day incubation period, as determined by gas chromatographic (GC). The degradation values were 68.9, 93.8 and 95.5% for Bacillus amyloliquefaciens, Kocuria rosea and the mixture of the tow isolates, respectively. In optimum conditions of pH 7, 40°C, 12 days incubation, the mixed bacterial consortium showed maximum sludge degradation.

scholarly journals Isolation and Characterization of Biosurfactant-producing Alcaligenes sp. YLA11 and its Diesel Degradation Potentials

2021 ◽  
Vol 9 (2) ◽  
pp. 7-12
Author(s):  
Abdulrahman Abdulhamid Arabo ◽  
Raji Arabi Bamanga ◽  
Mujiburrahman Fadilu ◽  
Musa Abubakar ◽  
Fatima Abdullahi Shehu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Incubation Time ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Diesel Oil ◽  
Contaminated Site ◽  
Screening Methods ◽  
Bacterial Strains ◽  
Isolation And Characterization ◽  
Degrading Bacteria

This study aimed to isolate and identify biosurfactant producing and diesel alkanes degrading bacteria. For this reason, bacteria isolated from the diesel contaminated site were screened for their potential to produce biosurfactants and degrade diesel alkanes. Primary selection of diesel degraders was carried out by using conventional enrichment culture technique where 12 bacterial strains were isolated based on their ability to grow on minimal media supplemented with diesel as sole carbon source, which was followed by qualitative screening methods for potential biosurfactant production. Isolate B11 was the only candidate that shows positive signs for drop collapse, foaming, haemolytic test, oil displacement of more than 22 ± 0.05 mm, and emulsification (E24) of 14 ± 0.30%. The effect of various culture parameters (incubation time, diesel concentration, nitrogen source, pH and temperature) on biodegradation of diesel was evaluated. The optimum incubation time was confirmed to be 120 days for isolates B11, the optimum PH was confirmed as 8.0 for the isolate, Similarly, the optimum temperature was confirmed as 35oC. In addition, diesel oil was used as the sole carbon source for the isolates. The favourable diesel concentration was 12.5 % (v/v) for the isolate. The isolate has shown degradative ability towards Tridecane (C13), dodecane, 2, 6, 10-trimethyl- (C15), Tetradecane (C14), 2,6,10-Trimethyltridecane (C16), Pentadecane (C15). It degraded between 0.27% - 9.65% individual diesel oil alkanes. The strain has exhibited the potential of degrading diesel oil n-alkanes and was identified as Alcaligenes species strain B11 (MZ027604) using the 16S rRNA sequencing.

scholarly journals Isolation and characterization of two bacterial strains from textile effluents having Malachite Green dye degradation ability

2020 ◽  
Author(s):  
Dipankar Chandra Roy ◽  
Md. Moinuddin Sheam ◽  
Md. Rockybul Hasan ◽  
Ananda Kumar Saha ◽  
Apurba Kumar Roy ◽  
...  
Keyword(s):  
Malachite Green ◽  
Large Scale ◽  
Dye Degradation ◽  
Inoculum Size ◽  
Bacterial Strains ◽  
Textile Effluents ◽  
Malachite Green Dye ◽  
Isolation And Characterization ◽  
Degrading Bacteria

AbstractWater pollution from textile effluent is now one of the major issues all over the world. Malachite Green dye of the triphenylmethane group is a key component of textile effluents. This study aimed to isolate and identify potential Malachite Green dye degrading bacteria from textile effluents. Different growth and culture parameters such as temperature, pH, inoculum-size and dye concentration were optimized to perform the dye-degradation assay using different concentrations of Malachite Green dye in mineral salt medium. A photo-electric-colorimeter was used to measure the decolorizing activity of bacteria at different time intervals after aerobic incubation. Two competent bacterial strains of Enterobacter spp. (CV-S1 and CM-S1) were isolated from textile effluents showing potential degradation efficiency against Malachite Green dye. The RAPD analysis and 16S rRNA sequencing confirmed the genetical difference of the isolated strains Enterobacter sp. CV–S1 and Enterobacter sp. CM–S1. The two bacterial strains CV-S1 and CM-S1 showed complete Malachite Green dye degradation up to 15 mg/l under shaking condition with 5% (v/v) inoculums at pH 6.50 and temperature 35°C within 72 and 144 hours respectively. These findings indicate that the two potential bacterial strains can be used in large scale treatment of textile effluents in the future.

scholarly journals Isolation and optimization of a glyphosate-degrading Rhodococcus soli G41 for bioremediation

2021 ◽  
Author(s):  
Tuan Ngoc Nguyen ◽  
Van Tam Vo ◽  
Phong The Hong Nguyen ◽  
Rudolf Kiefer
Keyword(s):  
Removal Rate ◽  
Cost Effective ◽  
Optimal Conditions ◽  
Bacterial Strains ◽  
Degrading Bacteria ◽  
Degradation Analysis ◽  
Soxb Gene ◽  
Glyphosate Degradation ◽  
High Removal Rate

Abstract A widely used herbicide for controlling weeds, glyphosate, is causing environmental pollution. It is necessary to remove it from environment using a cost-effective and eco-friendly method. The aims of this study were to isolate glyphosate-degrading bacteria and to optimize their degradative conditions required for bioremediation. Sixteen bacterial strains were isolated through enrichment and one strain, Rhodococcus soli G41, demonstrated a high removal rate of glyphosate than other strains. Response surface methodology was employed G41 strain to optimize distinct environmental factors on glyphosate degradation of G41 strain. The optimal conditions for the maximum glyphosate degradation were found to have the NH4Cl concentration of 0.663% and glyphosate concentration of 0.115%. Degradation analysis showed 47.1% of glyphosate in soil was degraded by G41 strain after 14 days. The presence of soxB gene in G41 strain indicates that the glyphosate is degraded via the eco-friendly sarcosine pathway. The results indicated that G41 strain has the potential to serve as an in-situ candidate for bioremediation of glyphosate polluted environments.

Biodegradation of organophosphorus Pollutants by Soil Bacteria: Biochemical Aspects and Unsolved Problems

2020 ◽  
Vol 36 (4) ◽  
pp. 126-135
Author(s):  
T.V. Shushkova ◽  
D.O. Epiktetov ◽  
S.V. Tarlachkov ◽  
I.T. Ermakova ◽  
A.A. Leontievskii
Keyword(s):  
Genome Sequencing ◽  
Soil Bacteria ◽  
Activity Regulation ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Russian Science ◽  
Degrading Bacteria ◽  
Phosphorus Source ◽  
Glyphosate Herbicide ◽  
Enzymatic Pathways

The degradation of persistent organophosphorus pollutants have been studied in 6 soil bacterial isolates and in 3 bacterial strains adapted for utilization of glyphosate herbicide (GP) under laboratory conditions. Significant differences in the uptake of organophosphonates were found in taxonomically close strains possessing similar enzymatic pathways of catabolism of these compounds, which indicates the existence of unknown mechanisms of activity regulation of these enzymes. The effect of adaptation for GP utilization as a sole phosphorus source on assimilation rates of several other phosphonates was observed in studied bacteria. The newly found efficient stains provided up to 56% of GP decomposition after application to the soil in the laboratory. The unresolved problems of microbial GP metabolism and the trends for further research on the creation of reliable biologicals capable of decomposing organophosphonates in the environment are discussed. organophosphonates, glyphosate, biodegradation, bioremediation, C-P lyase, phosphonatase, degrading bacteria Investigation of phosphonatase and genome sequencing were supported by Russian Science Foundation Grant no. 18-074-00021.

Sign in / Sign up

Export Citation Format

Share Document