scholarly journals Polyphenols Content and In Vitro α-Glycosidase Activity of Different Italian Monofloral Honeys, and Their Effect on Selected Pathogenic and Probiotic Bacteria

2021 ◽  
Vol 9 (8) ◽  
pp. 1694
Author(s):  
Florinda Fratianni ◽  
Maria Neve Ombra ◽  
Antonio d’Acierno ◽  
Lucia Caputo ◽  
Giuseppe Amato ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Probiotic Bacteria ◽  
Polyphenol Content ◽  
Total Polyphenols ◽  
E Coli ◽  
Glycosidase Activity ◽  
Probiotic Strains ◽  
Italian Origin ◽  
And Staphylococcus Aureus

We evaluated the polyphenol content and the α-glucosidase activity exhibited by different monofloral honeys of Italian origin. Their capacity to act on different pathogenic (Acinetobacter baumannii, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus) as well as probiotic bacteria (Lacticaseibacillus casei, Lactobacillus acidophilus, Lactiplantibacillus plantarum, Lactobacillus gasseri, and Lacticaseibacillus rhamnosus) was also assessed. Total polyphenols varied between 110.46 μg/g of fresh product (rhododendron honey) and 552.29 μg/g of fresh product (strawberry tree honey). Such result did not correspond to a parallel inhibitory α-glycosidase activity that, in each case was never higher than 33 μg/mL. Honeys were differently capable to fight the biofilm formation of the pathogens (inhibition up to 93.27%); they inhibited the in vitro adhesive process (inhibition up to 84.27%), and acted on mature biofilm (with values up to 76.64%). Their effect on bacterial metabolism was different too. Honeys were ineffective to inhibit E. coli mature biofilm nor to act on its metabolism. The action of the honey on probiotic strains seemed almost always stimulate their growth. Thus, these monofloral honeys might exhibit effects on human health and act positively as prebiotics.

Biofilm Formation on Voice Prostheses: In vitro Influence of Probiotics

2001 ◽  
Vol 110 (10) ◽  
pp. 946-951 ◽  
Author(s):  
Rolien H. Free ◽  
G. Jolanda Elving ◽  
Ranny van Weissenbruch ◽  
Henk J. Busscher ◽  
Henny C. vander Mei ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Saline Solution ◽  
Streptococcus Thermophilus ◽  
Probiotic Bacteria ◽  
Bacterial Suspension ◽  
Voice Prostheses ◽  
Colony Forming Units ◽  
Probiotic Strains ◽  
Phosphate Buffered Saline

In order to determine the influence of probiotic bacteria on biofilm formation on Groningen and Provox 2 voice prostheses in an artificial throat, we grew biofilms on both types of voice prostheses and exposed them 3 times daily to a probiotic bacterial suspension. As a control, we perfused an artificial throat with phosphate-buffered saline solution. Perfusion with Lactococcus lactis 53 suspension reduced the percentage numbers of bacteria and yeasts, respectively, on the Groningen prostheses to 17% and 22% and on the Provox 2 prostheses to 19% and 45%, compared to the number of colony-forming units on the control prosthesis, which was set at 100%. A suspension of Streptococcus thermophilus b reduced the percentage numbers of bacteria and yeasts, respectively, on the Groningen prostheses to 53% and 33% and on the Provox 2 prostheses to 14% and 0%, as compared to the control prosthesis. All other probiotic strains tested caused some reduction in the percentages of bacteria or yeasts, but strong differences between the types of prostheses were observed. In conclusion, L lactis 53 and S thermophilus b strongly reduce the occurrence of yeasts and bacteria in voice prosthetic biofilms.

Heat- and Formalin-Inactivated Probiotic Bacteria Induce Comparable Cytokine Patterns in Intestinal Epithelial Cell–Leucocyte Cocultures

2007 ◽  
Vol 70 (10) ◽  
pp. 2417-2421 ◽  
Author(s):  
ELEONORA DEHLINK ◽  
KONRAD J. DOMIG ◽  
CHRISTINE LOIBICHLER ◽  
ELKE KAMPL ◽  
THOMAS EIWEGGER ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Cytokine Production ◽  
Probiotic Bacteria ◽  
Intestinal Epithelial ◽  
Specific Production ◽  
Peripheral Blood Mononuclear ◽  
Probiotic Strains ◽  
Cytokine Synthesis

The mode of inactivation of probiotic bacteria may profoundly affect their immune-modulatory properties to the point of reversal of effects in in vitro human intestinal epithelial-like cell cultures (Caco-2). To further investigate the influence of inactivation treatment on cytokine production, three probiotic strains were evaluated—live, heat-inactivated, and formalininactivated strains—for their impact on interleukin (IL) 6, IL-8, and IL-10 production in Caco-2–leucocyte cocultures. The tested bacteria induced strain-specific production of IL-6, IL-8, and IL-10. No suppressive effects on cytokine synthesis were observed. Live microorganisms seemed to be slightly more potent inducers of cytokine production than nonviable strains, but differences to inactivated bacteria were not statistically significant. Our results indicate that heat and formalin treatments of probiotic microorganisms are equivalent inactivation methods in terms of induction of IL-6, IL-8, and IL-10 production in Caco-2–peripheral blood mononuclear cell cocultures and do not invert immune-modulatory effects.

Adherence of bacteria to mucus collected from different parts of the reproductive tract of heifers and cows

2013 ◽  
Vol 59 (11) ◽  
pp. 720-725 ◽  
Author(s):  
E. Styková ◽  
R. Nemcová ◽  
I. Valocký ◽  
F. Novotný ◽  
P. Guba
Keyword(s):  
Reproductive Tract ◽  
Fusobacterium Necrophorum ◽  
Gardnerella Vaginalis ◽  
Gastric Mucus ◽  
Strain Specificity ◽  
E Coli ◽  
Probiotic Strains ◽  
Lactobacillus Mucosae ◽  
Different Parts

In the present study, we examined the adherence of indigenous vaginal bacteria, probiotic strains, and metritis pathogens to mucus collected from different parts of the reproductive tracts of heifers and cows and compared their adherence with the bacterial adherence to mucus collected from the stomach and large intestine of pigs. Most of the vaginal strains adhered to mucus collected from different parts of the reproductive tract and strongly adhered to gastric mucus, with the exception of Lactobacillus buchneri 24S8. Only Lactobacillus mucosae 29S8, Enterococcus faecium E21, and E. faecium EAC adhered to colonic mucus. Probiotic strains adhered strongly to mucus collected from the reproductive tract and gastric mucus but did not adhere to colonic mucus. Pathogenic strains were adherent to vaginal, uterine horn, and gastric mucus, except Escherichia coli O8:K88ab:H9 (65), Fusobacterium necrophorum, and Gardnerella vaginalis, which adhered to uterine cervix mucus. Only Kocuria kristinae and G. vaginalis adhered to uterine body mucus; E. coli O149:K88ac (EC) adhered to colonic mucus. The strains did not exhibit host specificity but rather strain specificity. The ability to adhere to mucus was a characteristic unique to each strain. To our knowledge, this is the first report regarding in vitro adherence of GRAS (Generally Regarded As Safe) lactobacilli isolated from different sources to mucus collected from different parts of the reproductive tract.

Antibacterial activity and in vitro cytotoxicity evaluation of alginate-AgNP fibers

2016 ◽  
Vol 87 (11) ◽  
pp. 1377-1386 ◽  
Author(s):  
Xihui Zhao ◽  
Qun Li ◽  
Xiaowen Li ◽  
Yanzhi Xia ◽  
Bing Wang ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Treatment Time ◽  
Biological Activities ◽  
In Vitro Cytotoxicity ◽  
Cell Counting ◽  
Logarithmic Phase ◽  
E Coli ◽  
Alginate Fibers ◽  
And Staphylococcus Aureus

Biopolymer nanocomposites containing metal nanoparticles have attracted much attention due to their excellent properties and broad applications. In this work, alginate fibers embedded with silver nanoparticles (AgNPs) were prepared. The as-obtained alginate-AgNP fibers exhibited antibacterial activity against both Gram microorganisms of model microbes Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). A growth kinetic study with S. aureus and E. coli displayed the inhibition of bacterial growth at the logarithmic phase. The cytotoxic effect of the fibers in human cervical cancer (HeLa) cells was assessed by cell counting kit-8 (CCK-8) assay and flow cytometry. The as-prepared alginate-AgNP fibers, particularly with high amount and long treatment time, showed high cell-killing efficiency. These findings emphasize that such alginate-AgNP fibers with multifaceted biological activities are a promising material for applications in the textile or biomedical fields.

Rosemary and Tea Tree Essential Oils Exert Antibiofilm Activities In Vitro against Staphylococcus aureus and Escherichia coli

2020 ◽  
Vol 83 (7) ◽  
pp. 1261-1267
Author(s):  
TING LIU ◽  
JINGFAN WANG ◽  
XIAOMAN GONG ◽  
XIAOXIA WU ◽  
LIU LIU ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Biofilm Formation ◽  
Gas Chromatography Mass Spectrometry ◽  
Tea Tree ◽  
E Coli ◽  
Microdilution Method ◽  
Broth Microdilution Method

ABSTRACT The purpose of the present study was to determine the bioactive compounds in rosemary essential oil (REO) and tea tree essential oil (TEO) and to investigate their antibacterial and antibiofilm activities against Staphylococcus aureus and Escherichia coli in vitro. The MIC and MBC assays were performed to assess the antibacterial activity of these two EOs against S. aureus and E. coli with the broth microdilution method. A crystal violet assay was used to ascertain the effects of EOs on the biofilm formation of the test strains, and a tetrazolium bromide (MTT) assay was used to measure the level of inactivation of mature biofilms by EOs. Gas chromatography–mass spectrometry revealed 15 compounds in REO and 27 compounds in TEO, representing 97.78 and 98.13% of the total EO, respectively. Eucalyptol and α-pinene were found in high concentrations in REO, and the two major compounds in TEO were 4-terpineol and terpinolene. The MICs of REO for the two S. aureus and E. coli test strains were both 0.5 mg/mL, and the MICs of TEO for the two strains were both 0.25 mg/mL. Therefore, these EOs can significantly inhibit the formation of biofilms and induced morphological biofilm changes, as verified by scanning electron microscopy. Both EOs had destructive effects on the mature biofilm of the two test strains. TEO was more inhibitory than REO for biofilm formation by the two test strains. HIGHLIGHTS

Selection and Evaluation of Potential Probiotic Lactic Acid Bacteria and Bifidobacteria Isolated from Human Intestinal Tract

2014 ◽  
Vol 910 ◽  
pp. 137-140
Author(s):  
Chao Hui Xue ◽  
Lan Wei Zhang ◽  
Hong Bo Li ◽  
Shu Mei Wang
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Antimicrobial Activities ◽  
E Coli ◽  
Probiotic Potential ◽  
Probiotic Lactic Acid Bacteria ◽  
Probiotic Strains ◽  
Health Promoting ◽  
Culture Supernatants ◽  
Ht 29

Three Lactobacillus strains were screened on the basis of probiotic characteristics (i.e., resistance to low pH and bile salts, adhesion to the human gastrointestinal tract, inhibition of pathogenic strains). They further exhibited producing antimicrobial activities of non-acid molecule (s). In addition, antibacterial peptides were isolated and purified from the cell-free culture supernatants of these three probiotic strains. Based on TricineSDSPAGE, the antimicrobial peptide was approximately 10 kDa in size. After analyzing the sequence of the 16SrDNA regions of these three strains, they were identified asLactobacillus crispatus Lactobacillus rhamnosus and Lactobacillus rhamnosua GG.Using an in vitro system simulating gastric transit, our findings indicated that the three probiotic strains had the ability to tolerate gastroenteric environment and the adhesive capacity to HT-29 cells. It was demonstrated that the probiotic strains inhibited subsequent adhesion of E. coli to the HT-29 cell. Among the selected strains,L. rhamnosusF1333 showed a high probiotic potential and could be used in health-promoting food products.

scholarly journals Sensitivity of antibiotic resistant and antibiotic susceptible Escherichia coli, Enterococcus and Staphylococcus strains against ozone

2015 ◽  
Vol 13 (4) ◽  
pp. 1020-1028 ◽  
Author(s):  
Stefanie Heß ◽  
Claudia Gallert
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Enterococcus Casseliflavus ◽  
Subsurface Layers ◽  
Wastewater Samples ◽  
And Staphylococcus Aureus

Tolerance of antibiotic susceptible and antibiotic resistant Escherichia coli, Enterococcus and Staphylococcus strains from clinical and wastewater samples against ozone was tested to investigate if ozone, a strong oxidant applied for advanced wastewater treatment, will affect the release of antibiotic resistant bacteria into the aquatic environment. For this purpose, the resistance pattern against antibiotics of the mentioned isolates and their survival after exposure to 4 mg/L ozone was determined. Antibiotic resistance (AR) of the isolates was not correlating with higher tolerance against ozone. Except for ampicillin resistant E. coli strains, which showed a trend towards increased resistance, E. coli strains that were also resistant against cotrimoxazol, ciprofloxacin or a combination of the three antibiotics were similarly or less resistant against ozone than antibiotic sensitive strains. Pigment-producing Enterococcus casseliflavus and Staphylococcus aureus seemed to be more resistant against ozone than non-pigmented species of these genera. Furthermore, aggregation or biofilm formation apparently protected bacteria in subsurface layers from inactivation by ozone. The relatively large variance of tolerance against ozone may indicate that resistance to ozone inactivation most probably depends on several factors, where AR, if at all, does not play a major role.

scholarly journals Oral Administration of the Broad-Spectrum Antibiofilm Compound Toremifene Inhibits Candida albicans and Staphylococcus aureus Biofilm FormationIn Vivo

2014 ◽  
Vol 58 (12) ◽  
pp. 7606-7610 ◽  
Author(s):  
Kaat De Cremer ◽  
Nicolas Delattin ◽  
Katrijn De Brucker ◽  
Annelies Peeters ◽  
Soña Kucharíková ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Broad Spectrum ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Candida Krusei ◽  
Content Type ◽  
And Staphylococcus Aureus

ABSTRACTWe here report on thein vitroactivity of toremifene to inhibit biofilm formation of different fungal and bacterial pathogens, includingCandida albicans,Candida glabrata,Candida dubliniensis,Candida krusei,Pseudomonas aeruginosa,Staphylococcus aureus, andStaphylococcus epidermidis. We validated thein vivoefficacy of orally administered toremifene againstC. albicans and S. aureusbiofilm formation in a rat subcutaneous catheter model. Combined, our results demonstrate the potential of toremifene as a broad-spectrum oral antibiofilm compound.

scholarly journals EFFICACY OF CHAKRAMARDA PATRA SWARASA (CASSIA TORA LINN) AND DADHI IN KRIMIJA ATISARAM: A PRECLINICAL APPROACH

2020 ◽  
Vol 11 (5) ◽  
pp. 212-217
Author(s):  
Sreelekshmi S ◽  
Priya S ◽  
Priyalatha B
Keyword(s):  
Staphylococcus Aureus ◽  
Public Health Problem ◽  
Signs And Symptoms ◽  
Major Public Health Problem ◽  
Trial Group ◽  
Zone Of Inhibition ◽  
Scientific Validity ◽  
E Coli ◽  
And Staphylococcus Aureus

Diarrhoea is a major public health problem especially in developing countries. The signs and symptoms of Diarrhoea can nearly be co-related to Atisara. Diarrhoea caused by bacterial pathogens can be equated with the lakshanas seen in Krimija Atisara. Hetu vyadhi pratyanika chikitsa is explained by Acharya Sushruta for treating Krimija Atisara. In Yogamritam Atisara chikitsa Adhikara; it is explained that Chakramarda patra Swarasa, taken along with Dadhi is effective in Atisara. Hence, the study has been undertaken as a pre-clinical step- In vitro Agar well diffusion model, Microorganisms used for the study are E coli and Staphylococcus aureus. Two trial groups were included in this study; trial group 1- Chakramarda patra Swarasa alone, Trial group 2- Chakramarda patra Swarasa with Dadhi. Gentamicin was used as Standard. It was observed that the 2 trial groups showed Zone of inhibition against E coli and Staphylococcus aureus. Both the trial groups showed More Zone of inhibition against Staphylococcus aureus than E coli. Mean zone of inhibition of the 2 trial groups was found to be lower as compared to that of Mean zone of inhibition of the standard against E coli and Staphylococcus aureus. Student ’t’ test is used to analyze the data. The ‘t’ value is not statistically significant at the level of P < 0.05. Hence null hypothesis is accepted and alternate hypothesis is rejected.This article aims to give a scientific validity to usage of Chakramarda patra Swarasa with Dadhi in Krimija Atisara.

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